Differences in theories, application forms and evaluation standards about curative effect between traditional Chinese medicine and modern medicine lead to not only question safty and effectiveness but also hinder development and internationalization of traditional Chinese medicine. Combination of common problems in traditional Chinese new drug registration with experiences in research on traditional Chinese new drugs of prevention and treatment of coronary heart disease elucidate application value about theory of disease-syndrome combination and multi-objective optimization in several ways such as the indications positioning, preparation process optimization, preclinical efficacy evaluating and clinical assessmenting of efficacy and analysis development prospect.
Animals ; Biomedical Research ; Cardiovascular Diseases ; diagnosis ; drug therapy ; prevention & control ; Disease Models, Animal ; Drug Discovery ; Drugs, Chinese Herbal ; administration & dosage ; Humans

Massage prescriptions for treating infantile anorexia in Chinese Academic Journal Full-Text Database (CNKI, 1979-2012), Chinese Scientific and Technological Journal Full-Text Database (VIP, 1989-2012) and Wanfang Database (1990-2012) were collected. By using Chinese Medicine Inheritance Auxiliary Platform (Version 2.0) Software, 286 massage prescriptions for treatment of infantile anorexia were screened involved 76 acupoints, 20 commonly used acupoints, and 57 core acupoint combinations. Infantile Tuina specific points were used as main acupoints in massage therapy for infantile anorexia, and core acupoints covered Jizhu, Pi meridian, abdomen, Nei-Bagua, Zusanli (ST36), and Ban-men.
Acupuncture Points ; Acupuncture Therapy ; Anorexia ; therapy ; Data Mining ; Databases, Factual ; Humans ; Infant ; Massage ; Meridians ; Physical Therapy Modalities ; Software

Objective To observe the effect of acupuncture and herbs on risk factors and prognosis of transient ischemic attack (TIA).Methods 120 patients received treatment from March to May 2011 in Gaomi People's Hospital and the Hospital of Gaomi were randomly divided into three groups,with 40 cases in each.Acupuncture and Chinese medicine group was treated with needling Neiguan and administered with modified Tianmagouteng decoction,3 times/d; Chinese medicine group was treated with modified Tianmagouteng diction exclusively,3 times/d; Western medicine group was treated with Aspirin,0.1 g,1 times/d.The three groups were treated for 30 d of two therapeutic courses.TCM syndrome score,blood pressure,blood lipids,blood rheology,blood sugar changes in the three groups of patients were observed before and after the treatment.And follow-up of elapse rate and the incidence of stroke in a year were observed.Results After treatment,① in the acupuncture and Chinese medicine group: the total cholesterol was (5.21 ± 0.97)mmol/L,whole blood viscosity was [(4.26±0.66) 200 mPa · s,(5.69±0.15) 30 mPa · s,(9.72±2.33) mPa · s],fibrinogen was (2.12± 0.46)g/L,systolic blood pressure was (124.13±9.96) mm Hg,diastolic blood pressure was (79.23±7.50) mm Hg,blood glucose was (5.59 ±0.78) mmol/L; in the Chinese medicine group,the total cholesterol was (5.43± 1.04) mmol/L,whole blood viscosity was [(4.25±0.25) 200 mPa · s,(5.46±0.55) 30 mPa · s,(9.81± 2.01) 5 mPa · s],fibrinogenwas (2.32±0.33) g/L,systolic blood pressure was (129.78±14.25) mm Hg,diastolic blood pressure was (79.54± 7.65) mm Hg,blood glucose was (5.60± 0.75) mmol/L; in the western medicine group,the total cholesterol was (5.52± 1.16) mmol/ L,full blood viscosity was [(4.12±0.54) 200 mPa · s,(5.45± 0.65) 30 mPa · s,(9.09 ± 1.11) mPa · s],fibrinogen was (2.41 ± 0.44) g/L,systolic blood pressure was (129.83 ± 14.91) mm Hg,diastolic blood pressure was (80.72±7.47) mm Hg,blood glucose was (5.75 ± 0.61) mmol/L.all these results were improved significantly than those before the treatment in the same group (P＜0.05),and the acupuncture and Chinese medicine group improved better than the other two groups (P＜0.05).② In 1-year follow-up,TIA recurrence rate of stroke in the acupuncture group were lower than the other two groups.Conclusion Acupuncture and Chinese medicine treatment were not only be able to improve the clinical symptoms of patients with TIA and blood pressure,blood lipids,blood theology,blood sugar and other risk factors for TIA patients significantly,but also can effectively reduced the relapse rate and the incidence of stroke.

Objective To study the prognosis significance of intratumoral microvascular density(IMD)and the expression of vascular endothelial growth factor(VEGF)in malignant mesothelioma.Methods The data of 79 cases were reviewed of malignant mesothelioma confirmed by histology,immunohistochemistry and telectron microscope.CD34 and the expression of VEGF were detected by Immunohistochemical method.Results The age of the patients with malignant mesothelioma ranged from 11 to 79 years.The peak incidence of malignant mesothelioma was at the range of 40-50 years.The sex ratio of male to female was 1.78∶1.The survival period of the 79 cases was between 5 and 160 months with the medial survival of 23 months.According to histological structure,these cases were divided into epithelioid type(51 cases),sarcomatous type(20 cases)and mixed type(8 cases).For the VEGF positive expression,epithelioid type was 26 cases(50.98%),sarcomatous type was 13 cases(65.00)and mixed type was 5 cases(62.50%).Based on the mean IMD,the patients with malignant mesothelioma were divided into two groups.The survival time of the patients in the higher IMD group was significantly shorter than those in the lower IMD group.The analysis,on the relation of VEGF expression to IMD,as well as survival time and histological subtype,showed that VEGF expression was positively correlated to IMD(P=0.02,r=0.330 2).A significant difference existed between the survival of VEGF positive expression and negative expression(P0.05).Conclusion The results suggest that the prognosis of malignant mesothelioma probably related with IMD and VEGF expression.The higher index of IMD and the higher expression of VEGF might imply a worse prognosis.

Objective To constitute a higher expression system and to obtain the breast cancer cell strains in which BSP is stably expressed. Methods hBSP gene was subcloned from pB-hBSP vector by PCR. The PCR fragment was inserted into the eukaryon expression vector, pIRES2-EGFP, which allow exogenous protein and EGFP to express respectively. The recombinant vector, pIRES2-hBSP-EGFP, was transfected into human breast cancer cells with Lipofectamine TM 2000. The expression of symbol protein EGFP, could be conveniently observed with fluorescent microscope. Results The recombinant pIRES2-hBSP-EGFP plasmid was constituted and successfully transfected into breast cancer cells. In the breast cancer cell strain hBSP and EGFP were expressed. Conclusion The successful constitution and transfection of hBSP and EGFP nonfusion expression vector laid a foundation for the further study on the effect of BSP on breast cancer metastasizing to bone in vivo or in vitro.

Objective To observe the effect of treating chronic renal failure with self-made Huazhuo Baoyuan decoction.Methods 90 patients of chronic renal failure belonging to spleen and kidney yang deficiency and turbid toxic obstruction type diagnosed by TCM syndrome differentiation were randomly divided into a treatment group and a control group according to the date of reception.40 cases in the control group were treated with conventional western medicine,which were low-salt high-quality low-protein diet; to correct acid-base balance and electrolyte imbalance; to correct anemia; adding amino acids; to control blood sugar in patients with history of diabetes; to control blood pressure in patients with hypertension.50 patients in the treatment group were additionally treated with self-made Huazhuo Baoyuan decoction on the basis of the control group.Such indexes as symptom score,serum creatinine (Scr),blood urea nitrogen (BUN),endogenous creatinine clearance rate (Ccr),hemoglobin (Hb),liver function:aspartate aminotransferase (AST),alanine aminotransferase (ALT) ; lipids:total cholesterol(TC),triglyceride(TG),high density lipoprotein cholesterol(HDL-C),low-density lipoprotein cholesterol (LDL-C) were observed before and after the treatment.Results After treatment,total effective rate was 88.0％ and 67.5％ in the treatment group and the control group respectively,the difference was statistically significant (P=0.012).The value of Scr (292.95± 85.95) μmol/L,BUN (22.69 ± 9.84) mmol/L,Ccr (22.69 ±9.84)rn1/min,CH(6.23 ± 0.57)mmol/L,TG(1.70± 0.22)mmol/L,HDL-C(1.45 ± 0.04)mmol/L,LDL-C(3.30 ±0.60) mmol/L showed significant difference after the treatment than before in the treatment group (P＜0.05 or P＜ 0.01).Compared with the control group of after treatment [Scr (336.63 ± 90.77)μmol/L,BUN(10.47 ±2.71) mmol/L,Ccr (19.47 ± 6.71) rnl/min,CH (7.21 ± 0.62) mmol/L,TG (2.30 ± 0.27) mmol/L,HDL-C (0.87 ±0.05)mmol/L,LDL-C (5.60±0.70)mmol/L],the difference was statistically significant(P＜0.05).Conclusion Self-made Huazhuo Baoyuan decoction was effective and safe to treat chronic renal failure.

ObjectiveTo observe the effects of Shengqing-Jiangzhuo decoction combined with western medicine on metabolic syndrome.Methods120 patients with metabolic syndrome in Gaomi City People's Hospital of Traditional Chinese Medicine from March 2008 to July 2011 were randomly divided into a treatment group and a control group according to case number,with 60 cases in each group.The control group was treated with Western medicine individualized and symptomatic treatment,while on the basis of the control group the treatment group was additionally treated with Shengqing-Jiangzhuo decoction.Both groups were continuously treated for 2 months.The main symptoms of the two groups of patients before and after clinical efficacy as well as blood pressure,body mass index (BMI),waist-hip ratio (WHR) lipid levels,glycosylated hemoglobin (HbA1C) changes were observed.Results① The clinical efficacy:After treatment,dizziness,dry mouth,constipation,bloating,irritability and other symptoms improved significantly,with 85.00％ and 73.33％ of the total effective rate in treatment group and control group respectively; ② laboratory parameters detection:there was statistical difference between the control group and the treatment group in terms of systolic blood pressure (127.30±8.00) mm Hg,diastolic blood pressure (81.35±3.81) mm Hg,body mass index (23.3±1.48),waist-hip ratio (0.85 ± 0.07),as well as cholesterol (3.32 ± 0.81)mmol/L triglyceride (1.76 ±1.01) mmol/L,low-density lipoprotein cholesterol (2.20±0.72)mmol/L,high density lipoprotein cholesterol (1.39±0.14) mmol/L,glycosylated hemoglobin (6.58±0.82) mg/dl,(P＜0.05 or P＜0.01).Conclusion Shengqing-Jiangzhuo decoction significantly improved the clinical efficacy of metabolic syndrome.

Objective: To build total cost allocation model based on Time Driven Activity-based Costing (TDABC) and explore how to use it to improve the hospital cost accounting system. Methods: Take an ultrasonic cardiogram department of public hospital as a cost object to estimate the cost of medical service, as well as the efficiency of service and operating benefit analysis by using TDABC method. Results: Parts of routine services of the ultrasonic cardiogram departments having policy loss in a public hospital, the department’ s capacity are not fully utilized in a certain period. Conclusion: TDABC not only plays positive role in raising the level of hospital cost management, but also provides useful references and scientific basis for the formulation and revision of medical service fee and carring out the DRGs payment.

Objective To observe the changes of metallothionein (M T) in the rat flap with ischemia-reperfusion injury. Methods A ri ght lower abdominal island flap was created in Wistar rats . 16 rats were random ly divided into two groups: the control ( n =8) and the ischemia-reperfusion injury group ( n =8). The contents of MT and malondialdehyde (MDA) were assaye d by 109 Cd-hemoglobin saturation and thiobarbituric acid (TBA) methods. Th e activity of myeloperoxidase (MPO) was measured by colorimetry. Results At ischemia 8 h, reperfusion 12 h and 24 h, the level of MDA in rat i schemia-reperfusion injury group increased 41.7 %, 111.4 %, and 135.7 %, the ac tivity of MPO increased 72.1 %, 218.9 % and 296.0 %, the content of MT increased 42.6 %, 52.8 % and 102.3 %, respectively, compared with the control group ( P

The purpose of this study was to evaluate the value of Chinese herbal fumigation in the postoperative anal disease. The authors randomly divided 348 patients into treatment group and control group with 174 cases in each group. The treatment group was given to the Chinese herbal medicine hemorrhoids lotion for fumigation based on conventional anti infective therapy, routine dressing change and relaxing bowel. The control group was given to 1 000 mL 1: 5 000 potassium permanganate solution for sitz bath, fumigation based on conventional anti infective therapy, routine dressing change and relaxing bowel. The pain score, edema score, bleeding score, granulation tissue growth score and wound healing time of two groups were compared after operation. The results showed that the postoperative 6 h pain scores were higher in the two groups, the postoperative 3,5,7 d pain scores gradually decreased, the difference was statistically significant (P < 0.05). The difference of postoperative 6 h pain scores was no significant difference between the two groups, while postoperative 3,5,7 d pain scores in the treatment group were significantly lower than those in the control group (P < 0.05). 7 days after operation, anal margin of edema score and blood in the stool score in the treatment group were lower than those in control group, meat medicine growth score was higher than that of the control group, the difference had statistical meaning (P < 0.05). The healing time of two groups was respectively (13.89 + 2.78), (18.45 + 1.65) d (P < 0.05). This study suggested that Chinese herbal fumigation and washing could reduce the pain degree of patients, the anal margin of edema, and the blood in the stool, also could promote granulation tissue growth and shorten the time of wound healing, deserve the clinical expansion.
Adult ; Anal Canal ; surgery ; Digestive System Surgical Procedures ; adverse effects ; Drugs, Chinese Herbal ; administration & dosage ; Edema ; drug therapy ; etiology ; Female ; Hemorrhage ; drug therapy ; etiology ; Hemorrhoids ; Humans ; Male ; Middle Aged ; Pain, Postoperative ; drug therapy