Differences in theories, application forms and evaluation standards about curative effect between traditional Chinese medicine and modern medicine lead to not only question safty and effectiveness but also hinder development and internationalization of traditional Chinese medicine. Combination of common problems in traditional Chinese new drug registration with experiences in research on traditional Chinese new drugs of prevention and treatment of coronary heart disease elucidate application value about theory of disease-syndrome combination and multi-objective optimization in several ways such as the indications positioning, preparation process optimization, preclinical efficacy evaluating and clinical assessmenting of efficacy and analysis development prospect.
Animals ; Biomedical Research ; Cardiovascular Diseases ; diagnosis ; drug therapy ; prevention & control ; Disease Models, Animal ; Drug Discovery ; Drugs, Chinese Herbal ; administration & dosage ; Humans

Massage prescriptions for treating infantile anorexia in Chinese Academic Journal Full-Text Database (CNKI, 1979-2012), Chinese Scientific and Technological Journal Full-Text Database (VIP, 1989-2012) and Wanfang Database (1990-2012) were collected. By using Chinese Medicine Inheritance Auxiliary Platform (Version 2.0) Software, 286 massage prescriptions for treatment of infantile anorexia were screened involved 76 acupoints, 20 commonly used acupoints, and 57 core acupoint combinations. Infantile Tuina specific points were used as main acupoints in massage therapy for infantile anorexia, and core acupoints covered Jizhu, Pi meridian, abdomen, Nei-Bagua, Zusanli (ST36), and Ban-men.
Acupuncture Points ; Acupuncture Therapy ; Anorexia ; therapy ; Data Mining ; Databases, Factual ; Humans ; Infant ; Massage ; Meridians ; Physical Therapy Modalities ; Software

Objective To observe the effect of acupuncture and herbs on risk factors and prognosis of transient ischemic attack (TIA).Methods 120 patients received treatment from March to May 2011 in Gaomi People's Hospital and the Hospital of Gaomi were randomly divided into three groups,with 40 cases in each.Acupuncture and Chinese medicine group was treated with needling Neiguan and administered with modified Tianmagouteng decoction,3 times/d; Chinese medicine group was treated with modified Tianmagouteng diction exclusively,3 times/d; Western medicine group was treated with Aspirin,0.1 g,1 times/d.The three groups were treated for 30 d of two therapeutic courses.TCM syndrome score,blood pressure,blood lipids,blood rheology,blood sugar changes in the three groups of patients were observed before and after the treatment.And follow-up of elapse rate and the incidence of stroke in a year were observed.Results After treatment,① in the acupuncture and Chinese medicine group: the total cholesterol was (5.21 ± 0.97)mmol/L,whole blood viscosity was [(4.26±0.66) 200 mPa · s,(5.69±0.15) 30 mPa · s,(9.72±2.33) mPa · s],fibrinogen was (2.12± 0.46)g/L,systolic blood pressure was (124.13±9.96) mm Hg,diastolic blood pressure was (79.23±7.50) mm Hg,blood glucose was (5.59 ±0.78) mmol/L; in the Chinese medicine group,the total cholesterol was (5.43± 1.04) mmol/L,whole blood viscosity was [(4.25±0.25) 200 mPa · s,(5.46±0.55) 30 mPa · s,(9.81± 2.01) 5 mPa · s],fibrinogenwas (2.32±0.33) g/L,systolic blood pressure was (129.78±14.25) mm Hg,diastolic blood pressure was (79.54± 7.65) mm Hg,blood glucose was (5.60± 0.75) mmol/L; in the western medicine group,the total cholesterol was (5.52± 1.16) mmol/ L,full blood viscosity was [(4.12±0.54) 200 mPa · s,(5.45± 0.65) 30 mPa · s,(9.09 ± 1.11) mPa · s],fibrinogen was (2.41 ± 0.44) g/L,systolic blood pressure was (129.83 ± 14.91) mm Hg,diastolic blood pressure was (80.72±7.47) mm Hg,blood glucose was (5.75 ± 0.61) mmol/L.all these results were improved significantly than those before the treatment in the same group (P＜0.05),and the acupuncture and Chinese medicine group improved better than the other two groups (P＜0.05).② In 1-year follow-up,TIA recurrence rate of stroke in the acupuncture group were lower than the other two groups.Conclusion Acupuncture and Chinese medicine treatment were not only be able to improve the clinical symptoms of patients with TIA and blood pressure,blood lipids,blood theology,blood sugar and other risk factors for TIA patients significantly,but also can effectively reduced the relapse rate and the incidence of stroke.

Objective To observe the changes of metallothionein (M T) in the rat flap with ischemia-reperfusion injury. Methods A ri ght lower abdominal island flap was created in Wistar rats . 16 rats were random ly divided into two groups: the control ( n =8) and the ischemia-reperfusion injury group ( n =8). The contents of MT and malondialdehyde (MDA) were assaye d by 109 Cd-hemoglobin saturation and thiobarbituric acid (TBA) methods. Th e activity of myeloperoxidase (MPO) was measured by colorimetry. Results At ischemia 8 h, reperfusion 12 h and 24 h, the level of MDA in rat i schemia-reperfusion injury group increased 41.7 %, 111.4 %, and 135.7 %, the ac tivity of MPO increased 72.1 %, 218.9 % and 296.0 %, the content of MT increased 42.6 %, 52.8 % and 102.3 %, respectively, compared with the control group ( P

Objective To observe the effect of treating chronic renal failure with self-made Huazhuo Baoyuan decoction.Methods 90 patients of chronic renal failure belonging to spleen and kidney yang deficiency and turbid toxic obstruction type diagnosed by TCM syndrome differentiation were randomly divided into a treatment group and a control group according to the date of reception.40 cases in the control group were treated with conventional western medicine,which were low-salt high-quality low-protein diet; to correct acid-base balance and electrolyte imbalance; to correct anemia; adding amino acids; to control blood sugar in patients with history of diabetes; to control blood pressure in patients with hypertension.50 patients in the treatment group were additionally treated with self-made Huazhuo Baoyuan decoction on the basis of the control group.Such indexes as symptom score,serum creatinine (Scr),blood urea nitrogen (BUN),endogenous creatinine clearance rate (Ccr),hemoglobin (Hb),liver function:aspartate aminotransferase (AST),alanine aminotransferase (ALT) ; lipids:total cholesterol(TC),triglyceride(TG),high density lipoprotein cholesterol(HDL-C),low-density lipoprotein cholesterol (LDL-C) were observed before and after the treatment.Results After treatment,total effective rate was 88.0％ and 67.5％ in the treatment group and the control group respectively,the difference was statistically significant (P=0.012).The value of Scr (292.95± 85.95) μmol/L,BUN (22.69 ± 9.84) mmol/L,Ccr (22.69 ±9.84)rn1/min,CH(6.23 ± 0.57)mmol/L,TG(1.70± 0.22)mmol/L,HDL-C(1.45 ± 0.04)mmol/L,LDL-C(3.30 ±0.60) mmol/L showed significant difference after the treatment than before in the treatment group (P＜0.05 or P＜ 0.01).Compared with the control group of after treatment [Scr (336.63 ± 90.77)μmol/L,BUN(10.47 ±2.71) mmol/L,Ccr (19.47 ± 6.71) rnl/min,CH (7.21 ± 0.62) mmol/L,TG (2.30 ± 0.27) mmol/L,HDL-C (0.87 ±0.05)mmol/L,LDL-C (5.60±0.70)mmol/L],the difference was statistically significant(P＜0.05).Conclusion Self-made Huazhuo Baoyuan decoction was effective and safe to treat chronic renal failure.

BACKGROUND: Bone marrow mesenchymal stem cells (BMSCs) are in advantages of easy collection and amplification in vitro, but the culture and induction in vitro still need to be modified. OBJECTIVE: To investigate the differentiated potency of BMSCs into ostcoblasts by modified in vitro culture methods and inductor matching. DESIGN: Observational study. SETTING: Department of Medical Laboratory, General Hospital of Guangzhou Military Area Command of Chinese PLA. MATERIALS: This study was performed in the Stem Cell Tissue Engineering Laboratory, Department of Medical Laboratory, Genera Hospital of Guangzhou Military Area Command of Chinese PLA from July 2004 to June 2006. Twenty adult SD rats of clean grade, irrespective of gender, weighing 140-180 g were provided by Animal Experimental Center, General Hospital of Guangzhou Military Area Command of Chinese PLA. The experimental animals were disposed according to ethical criteria. Β-sodium glycerophosphate, dexamethasone, and vitamin C were provided by Sigma Company, USA; goat-anti-rat osteocalcin antibody by DSL Company, USA; S-P immunohistochemical kit by Maixin Biotechnology Developing Co., Ltd., Fujian. METHODS: Cells were cultured and induced into osteoblasts by modified methods. Separation and culture of BMSCs: By anesthesia, bone marrow of bilateral femur and tibia was separated to prepare single cell suspension; subsequently, the suspension was inoculated in culture bottle, and the culture media was semi-quantitatively changed after 48 and 96 hours in order to get rid of non-adherent hematopoietic cells. The liquid was changed every three days to further get rid of non-adherent cells. At 80% cell confluence, the medium was digested with 0.25% trypsin and cells were subcultured in the ratio of 1:2. MSCs in the second generation were inoculated in 6-well culture plate and glass fiat plate; after 48 hours, basic culture fluid was removed. Differentiation of BMSCs into osteoblasts: Subcultured BMSCs differentiated into osteoblasts induced by dexamethasone (10 mmol/L), β-sodium glycerophosphate (10-7 mol/L), and vitamin C (50 mg/L). MAIN OUTCOME MEASURES: Ten days after differentiation by modified culture methods and inductor matching, alkaline phosphatase activity was measured with Ca-Co technique. Twelve days after culture, osteocalcin secretion was detected with immunohistochemical method. Two weeks after culture, cell mineralization was detected with Von kossa staining. RESULTS: Alkaline phosphatase activity: Alkaline phosphatase staining of cells was apparent; gray-black particles or massive precipitations were observed in cytoplasm after positive reaction; regions expressing alkaline phosphatase activity were brown-black. Osteocalcin secretion: Osteocalcin was apparently positive; nucleus was blue; cytoplasm was brown. Cell mineralization: Induced cells grew layer by layer, and adiaphanous nodus was observed at the same time. Black mineralized nodus granules in various sizes were observed after Von kossa staining, and this suggested that mineralized apposition occurred. CONCLUSION: BMSCs may be successfully cultured and induced into osteoblasts by modified culture method.

BACKGROUND:With the potential of multi-directional differentiation and high proliferation, bone marrow mesenchymal stem cells (BMSCs) have wide application prospect in tissue engineering. OBJECTIVE:To investigate changes in cells and bioactivity in the differentiation from BMSCs into osteoblasts. DESIGN, TIME AND SETTING:A randomized control experiment was performed at the Laboratory of Stem Cells and Tissue Engineering, Department of Medical Experiment, General Hospital of Guangzhou Military Area Command of Chinese PLA from July 2004 to June 2006. MATERIALS:Twenty adult SD rats of both genders were used for bone marrow collection. METHODS:Rats were equally and randomly divided into an experimental group and a control group. BMSCs were isolated from adult rats by modified bone marrow culture method. BMSCs were inoculated in basic medium containing 10% fetal bovine serum, high glucose DMEM, 100 U/L penicillin, 100 U/L streptomycin and 2 mmol/L glutamine in the control group. BMSCs were inoculated in conditioned medium (basic medium, 10 mmol/L β-glycerophosphoric sodium, 10-7 mol/L dexamethasone, 50 mg/L vitamin C). Cell slide was prepared. MAIN OUTCOME MEASURES:Inverted microscope and transmission electron microscope were used to observe cell appearance. Gomori modified calcium-cobalt method was applied to do alkaline phosphatase staining. Immunocytochemistry was employed to measure osteocalcin expression. RESULTS:Forty-eight hours after inoculation, a mass of BMSCs adhered to a wall. Seventy-two hours later, BMSCs proliferated and well grew. Seven to nine days later, cells were grown to 80% confluency. Transmission electron microscope showed BMSCs with big cell nucleus and immature appearance. After in vitro osteoblast induction, BMSCs proliferated, aggregated, had node and mineralized. One week later, alkaline phosphatase activities were expressed in BMSCs. Two weeks later, osteocalcin expression was detected. CONCLUSION:After one week of in vitro osteogenic induction, BMSCs enter the process of osteoblast transformation, remain proliferative activities, and can be used as seed cells in bone tissue engineering

ObjectiveTo observe the effects of Shengqing-Jiangzhuo decoction combined with western medicine on metabolic syndrome.Methods120 patients with metabolic syndrome in Gaomi City People's Hospital of Traditional Chinese Medicine from March 2008 to July 2011 were randomly divided into a treatment group and a control group according to case number,with 60 cases in each group.The control group was treated with Western medicine individualized and symptomatic treatment,while on the basis of the control group the treatment group was additionally treated with Shengqing-Jiangzhuo decoction.Both groups were continuously treated for 2 months.The main symptoms of the two groups of patients before and after clinical efficacy as well as blood pressure,body mass index (BMI),waist-hip ratio (WHR) lipid levels,glycosylated hemoglobin (HbA1C) changes were observed.Results① The clinical efficacy:After treatment,dizziness,dry mouth,constipation,bloating,irritability and other symptoms improved significantly,with 85.00％ and 73.33％ of the total effective rate in treatment group and control group respectively; ② laboratory parameters detection:there was statistical difference between the control group and the treatment group in terms of systolic blood pressure (127.30±8.00) mm Hg,diastolic blood pressure (81.35±3.81) mm Hg,body mass index (23.3±1.48),waist-hip ratio (0.85 ± 0.07),as well as cholesterol (3.32 ± 0.81)mmol/L triglyceride (1.76 ±1.01) mmol/L,low-density lipoprotein cholesterol (2.20±0.72)mmol/L,high density lipoprotein cholesterol (1.39±0.14) mmol/L,glycosylated hemoglobin (6.58±0.82) mg/dl,(P＜0.05 or P＜0.01).Conclusion Shengqing-Jiangzhuo decoction significantly improved the clinical efficacy of metabolic syndrome.

Objective To observe the effects of self-made Yicong-Shugan drink in the treatment of anxiety disorders before the college entrance examination.Methods Random number table was adopted for dividing 121 outpatients with anxiety disorders before the college entrance examination in the People's Hospital of Gaomi and Gaomi TCM Hospital since January 2007 to March 2011 into a treatment group with 69 cases,and a control group with 52 cases.The treatment group was treated with Yicong-Shugan Decoction,while the control group was given alprazolam.Hamilton Anxiety Scale (HAMA),the Clinical Global Impression Scale (CGI) and Anxiety Rating Scale (SAS) were used to determine the clinical efficacy in both groups before treatment and 1,2,4,6 weeks after the treatment.Results ① In the control group after treatment,one week HAMA score (23.1 ±7.9) was significantly changed than before the treatment (31.1 ±7.6) (P＜0.05); whereas no significant change in the treatment group (P＜0.05).This demonstrated that alprazolam has a faster affection than Yicong-Shugan drink.② HAMA integral scores,CGI integral scores and SAS scores of both groups at 2,4,6 weeks after treatment were:HAMA integral score:(22.6 ± 7.5),(11.7 ± 5.3),(7.9 ± 5.4) in the treatment group,while (18.7 ±6.9),(13.8 ±6.7),(11.8 ±6.5) in the control group; CGI integral score:(3.4 ±0.7),(2.1 ±0.6),(1.8 ±0.7) in the treatment group,while (3.2 ±0.8),(1.8±0.5),and (1.6 ±0.6) in the control group; the SAS scores:(53.8±8.5),(38.7 ±9.6),(30.6 ±7.6) in the treatment group,while (50.2 ±8.9),(41.5 ±9.8),and (39.2 ±8.3) in the control group respectively.All these scores showed significant difference than those before the treatment in the same group (P＜0.01).HAMA and SAS scores in the treatment group were significantly lower than the control group after the treatment (P＜0.01).This demonstrated that both alprazolam tablets and Yicong-Shugan drink have a good anxiolytic effect,but Yicong-Shuxin drink has a longer lasting effects.Conclusion Self made Yicong-Shugan drink can effectively improve the patient with college entrance examination anxiety disorder.Alprazolam tablets have a quicker effects than self-made Yicong-Shugan decoction,while Yicong-Shuxin decoction has a longer lasting effects than alprazolam.

Objective: To build total cost allocation model based on Time Driven Activity-based Costing (TDABC) and explore how to use it to improve the hospital cost accounting system. Methods: Take an ultrasonic cardiogram department of public hospital as a cost object to estimate the cost of medical service, as well as the efficiency of service and operating benefit analysis by using TDABC method. Results: Parts of routine services of the ultrasonic cardiogram departments having policy loss in a public hospital, the department’ s capacity are not fully utilized in a certain period. Conclusion: TDABC not only plays positive role in raising the level of hospital cost management, but also provides useful references and scientific basis for the formulation and revision of medical service fee and carring out the DRGs payment.