AIM: To establish the technique of precision-cut fibrotic liver slice (PCLS) and grope the optimal cultural conditions for researching the liver xenobiotic metabolism in vitro and the drug interaction. METHODS: Complex factors (higher fat diet, alcohol and CCl 4) were used to make the animal model of liver fibrosis. Fibrotic liver slices were prepared and cultivation system was established. Lactate dehydrogenase (LDH) leakage, glutathione S-transferase (GST) activity and 3[4,5-Dimethythiazole-2-yl]-2,5- diphenyltetrazolium bromide (MTT) reduction were chosen as indexes to assess the viability of the slice in different thickness, medium pH and cultural time. RESULTS: Rats were in earlier hepatic fibrosis after administration for 3 weeks. When the thickness of slices was 300 ?m and medium pH was 7.0, the LDH leakage, GST activity and MTT reduction could maintain on a steady level in 6 h. CONCLUSION: A 300 ?m of thickness, 7.0 of medium pH and 6 h of cultural time are the optimal slicing and culturing conditions for fibrotic liver slice.

Analysis of metabolic activities of cytochrome P450 isoenzyme is a crucial index to study drug/toxicant metabolism, drug-drug interaction, polymorphism and et al. Due to this practice, it is important to use the proper probe substrate and to conduct the experiment under optimal conditions. The validation information in literatures on the most common and newest in vitro probe substrates have been reviewed.

Thymomas are most frequent primary tumors of anterior mediastinum.Surgery is the principal treatment method.Thymomas are sensitive to chemotherapy,and chemotherapy has showed a sig- nificant anti-tumor effect in locally advanced,recurrent or metastatic thymomas.Chemotherapy,radiother- apy or combined chemotherapy and radiotherapy before or after operation can offer patients excellent re- sponses,improve resectable rate.Some patients who are once inoperable obtain curative and palliative operation.Radiotherapy after operation can decrease residual tumor tissue,increase local control,de- crease recurrence,and prolong the survival.The multimodality therapy plays an important role in thy- moma treatment.

This article introduced the experiences of nongovernmental capital running hospitals in US,Germany,India and Israel and put forward some suggestions that need to be considered in China, such as innovating supply model of health care service,treating social capital running hospitals equally and strengthening supervision.

Objective To investigate the relation of Toll-like receptor 4 (TLR4)and monocyte chemoattractant protein-1 (MCP-1)with acute coronary syndrome.Methods 60 patients with coronary heart disease patients including 39 cases of a-cute coronary syndrome (ACS group),21 cases of stable angina (SA group)from January 2015-March 2016 in Xi’an First Hospital were in this observation.ACS group was divided into acute myocardial infarction (AMI,n=21)and unstable angina pectoris (UAP,n=18).Another 30 cases with angiographically normal was included in control group.Enzyme-linked immu-nosorbent assay (ELISA)detection was used to detect the serum content of MCP-1,TLR4 expression was tested by flow cy-tometry.Triglyceride (TG),total cholesterol (TC),low-density lipoprotein cholesterol (LDL-C)and high-density lipopro-tein cholesterol (HDL-C)were detected.Results TLR4 levels of ACS group was significantly higher than SA group (t=4.455,P=0.021).Serum MCP-1 levels of ACS group were significantly higher than SA group (t=3.220,P=0.002)and control group (t=6.197,P=0.000).MCP-1 levels of ACS group were higher than control group (t=2.306,P=0.025). The Spearman correlation test TLR4 was positively with MCP-1 (r=2.389,P=0.025).Conclusion MCP-1 and TLR4 may determine the severity of coronary heart disease,combined detection of MCP-1 and TRL4 can provide the clinical value for early diagnosis and treatment of ACS.

Aneurysm ; diagnosis ; Carotid Artery, Internal ; pathology ; Diagnostic Errors ; Female ; Humans ; Hypertrophy ; diagnosis ; Middle Aged ; Palatine Tonsil ; pathology

Objective:To evaluate the effect of preoxidation on the bonding strength of titanium to porcelain.Method:12 titanium plates(25 mm?3 mm?0.5 mm) were pre-oxidated according to usual preoxidation procedure and 12 without preoxidation were used as the controls.The samples(6 in each group) were then bonded with bonding porcelain,opaque porcelain,dentin porcelain and glaze of Duceratin(Degussa) or Vita titankeramik(Vita) respectively.The bonding strength of Ti/porcelain was evaluated using three-point bending test according to ISO 9693 1990 standard. The interface of Ti/porcelain bonding was observed by SEM.Results:Bonding strength(MPa) of Ti/Duceratin preoxided group and the corresponding control was 41.910?2.778 and 33.097?5.297(P0.05),respectively.SEM observation showed inter-lock and tight bonding of Ti/porcelain in pre-oxidated interface,more cracks and gaps between Ti and porcelain in non-pre-oxidated interface.Conclusion:Pre-oxidation may improve bonding strength of Ti/Duceratin system, while has no significant effect on bonding strength of Ti/Vita titankeramik system.

0.05).Statistical analysis showed that there was a correlation between the expression of CTGF and TGF-?RⅡ,FN,and collagen Ⅰ and Ⅲ protein,respectively. Conclusions The expression of CTGF and TGF-?RⅡ protein is up-regulated in PRM of PVR,which suggests that the activation of TGF-?RⅡ is involved in the production of CTGF,and CTGF is closely related to the production of ECM and play an important role in the pathogenesis of PVR.

Objective To investigate the clinical characteristics and therapy of acute perforation of gastric carcinoma(APGC) to improve the diagnosis,treatment and prognosis of APGC. Methods Forty cases of APGC admitted to Gong liao hospital of Zhenping county.Of the forty patients,ten underwent repair of perforation,twenty-one cases underwent palliative gastrectomy (six of them underwent lymphadenectomy three weeks after previous gastrectomy );five subjected to radical gastrectomy. two underwent gastrostomy or jejunostomy for nutrition.One underwent drainage and peritoneal lavage only;1 received non-operative therapy.Results Four patients died in this series.The 1-,2-,3-years survival rate was 83.3%(30/36),52.8%(19/36),27.8%(10/36)respectively. Conclusions Early diagnosis and adopting resection of gastric cancer plus comperhensive treatment can improve the prognosis of patients with APGC.

Animals ; Female ; Hydrocarbons ; toxicity ; Kidney ; drug effects ; ultrastructure ; Liver ; drug effects ; ultrastructure ; Male ; Mice