Objective: To evaluate the associations of manganese superoxide dismutase (MnSOD) genetic polymorphism with the susceptibilities of prostate, esophageal and lung cancers. Methods: Studies were identified by searching computerized databases (Cochrane Library, PubMed, etc.), accompanied by manual search. The case-control studies were selected according to defined inclusion and exclusion criteria. After quality evaluation and data abstraction, a meta-analysis was performed by using STATA 11.0 software. Results: A total of 22 case-control studies were eligible for this analysis, including 8 181 cases and 11 844 healthy controls. For prostate cancer, 12 case-control studies included 4 182 cases and 6 885 healthy controls. Meta-analysis showed that MnSOD polymorphism could significantly increase the risk of prostate cancer [heterozygote genotype: odds ratio (OR)=1.11, 95% confidence interval (CI)=1.01-1.22; homozygote genotype: OR=1.25, 95%CI=1.03-1.51); dominant genotype: OR=1.15, 95%CI=1.01-1.31)]. For esophageal cancer, 4 case-control studies contained 620 cases and 909 healthy controls. Meta-analysis showed that MnSOD polymorphism could significantly increase the risk of esophageal cancer [heterozygote genotype: OR=1.58, 95%CI=1.22-2.04; homozygote genotype: OR=2.25, 95%CI=1.61-3.15); recessive genotype: OR=1.69, 95%CI=1.07-2.67); dominant genotype: OR=1.74, 95%CI=1.36-2.22)]. For lung cancer, 6 case-control studies contained 3 375 cases and 4 050 healthy controls. Meta-analysis showed that MnSOD polymorphism could significantly decrease the risk of lung cancer [homozygote genotype: OR=0.68, 95%CI=0.59-0.78); recessive genotype: OR=0.71, 95%CI=0.54-0.93); dominant genotype: OR=0.83, 95%CI=0.75-0.92)]. Conclusion: MnSOD polymorphism is associated with elevated risks of prostate and esophageal cancers, but decreased risk of lung cancer. Copyright© 2011 by Tumor.

Objective: To explore the effect of manganese superoxide dismutase (MnSOD) overexpression on the proliferation of esophageal cancer cell line TE-1 in vivo and in vitro . Methods: TE-1 cells were transfected with a plasmid binding MnSOD cDNA, and then TE-1Mm cells (with moderate expression of MnSOD) and TE-1Mh cells (with high expression of MnSOD) were established. RT-PCR and Western blotting were used to detect the mRNA and protein expressions of target MnSOD gene in both of TE-1Mm and TE-1Mh cells, respectively. The ability of cell proliferation and apoptosis and the cell cycle distribution were examined by plate colony-forming test and flow cytometry (FCM). The xenograft tumor models in nude mice were established. The effect of MnSOD overexpression on cell proliferation was evaluated in vivo , and the expression of MnSOD protein in xenograft tumors was detected by immunohistochemistry and Western blotting. Results: TE-1 cells with different expression levels of MnSOD proteins were established by transfection with different amounts of plasmids binding MnSOD cDNA. The colony-formation rates of TE-1Mm and TE-1Mh cells were (23.0±2.7)% and (45.3±4.5)%, respectively, which were both significantly higher than those in TE-1 cells (34.7±4.2)% and TE-1n cells (33.7±4.7)%, P<0.05. The apoptosis rate of TE-1Mm (10.6±1.0)% was significantly higher than those in TE-1 cells (34.7±4.2)% and TE-1n cells (33.7±4.7)%, and the apoptosis rate of TE-1Mh (10.6±1.0)% was significantly lower than those in TE-1 cells and TE-1n cells (P<0.05). FCM revealed that the percentage of TE-1Mh cells was decreased in G 0/G1 phase and increased in G2/M and S phases, while the percentage of TE-1Mm cells was increased in G0/G 1 phase and decreased in G2/M and S phases induced by MnSOD overexpression. The growth of xenograft tumors was inhibited in TE-1Mm cell-implanted group, while which was improved in TE-1Mh cell-implanted group. The expressions of MnSOD protein in TE-1Mm and TE-1Mh cells were significantly higher than those in TE-1 and TE-1n cells (P<0.05). Conclusion: MnSOD overexpression exerts a two-way effect involving inhibition or promotion on the proliferation of TE-1 cells in vivo and in vitro.

Electrocardiography ; Humans ; Takotsubo Cardiomyopathy ; Ventricular Dysfunction, Left

Objective To investigate the effect of sodium fluoride(NaF) on proliferation, differentiation and the mRNA expression of osteoprotegerin (OPG) and receptor activator of nuclear factor κβ ligand (RAN KL) of mouse osteoblasts. Methods Osteoblasts were isolated from calvarias of Kunming mice born in 1 - 2 d and cultured. Various concentrations of NaF(0, 10-8, 10-7, 10-6, 10-5, 10-4, 10-3mol/L) were added to the culture medium, the proliferation and activity of alkaline phosphatase(ALP) was determined after 72 h or 120 h. The expression of OPG mRNA and RANKL mRNA was analyzed by semi-quantification RT-PCR. Difference among groups was analyzed by One-Way AN0VA. Difference between two groups was analyzed by LSD-t test. Results There was significant difference in cell proliferation among groups after 72 h(F = 13.806, P < 0.05). Compared with control group(0.434 ± 0.010) , the proliferation was significantly induced in 10-7 - 10-4 mol/L groups treated osteoblasts (0.448 ± 0.010, 0.453 ± 0.013, 0.454 ± 0.016, 0.449 ± 0.018, all P< 0.05), and was significantly suppressed in 10-3 mol/L group(0.401 ± 0.009, P < 0.05). There was statistic difference in the activity of ALP among groups(F = 9.021, P < 0.05). Compared with control group (1.677 ± 0.682), the activity of ALP significantly increased in 10-7 - 10-5 mol/L groups[ (2.447 ± 0.756) × 106, (2603 ± 0.183) × 106, (2.687 ± 0.886) × 106 U/L, P < 0.05 or P < 0.01 ] and significantly decreased in 10-4 mol/L group[ (1.479 ± 0.366) × 106 U/L, P < 0.05 ]. There was significant difference in the expression of OPG mRNA among groups(F = 11.299, P< 0.05). Compared with control group (1.000 ± 0.000), the expression of OPG mRNA was significantly increased in 10-7 - 10-4 mol/L groups( 1.058 ± 0.027, 1.053 ± 0.026, 1.088 ± 0.055, 1.069 ± 0.008, P < 0.05 or P < 0.01) , while significantly decreased in 10-3 mol/L group (0.941 ± 0.029, P< 0.05). There was no difference in RANKL mRNA expression among groups (F= 1.311, P> 0.05). The ratio of RANKL/OPG decreased with increasing doses of fluoride and increased in 10-4, 10-3 mol/L groups, but there was no difference between groups(F = 1.376, P> 0.05). Conclusions A biphasic pattern of proliferation and differentiation has been induced in mouse osteoblasts, which manifests stimulation effect in low doses and suppression in higher doses. Low doses of sodium fluoride suppress differentiation and maturation of osteoblasts by increasing expression of OPG mRNA, while high doses of sodium fluoride enhance differentiation and maturation of osteoblasts by decreasing expression of OPG mRNA.

Drug-Eluting Stents ; adverse effects ; Humans ; Middle Aged ; Myocardial Infarction ; etiology ; Sirolimus ; administration & dosage ; Thrombosis ; etiology

Objective To study the impact of preprocedure intravenous urography (IVU) on the extracorporeal shock wave lithotripsy(ESWL) for proximal ureteral stones.Methods One hundred patients with solitary radiopaque proximal ureteral stones on plain radiographs and no severe hydronephrosis on ultrasonographic examination were allocated randomly to two treatment groups.IVU group (n=50) had IVU before the start of ESWL,whereas patients in control group (n=50) underwent ESWL without IVU.Postop- erative success,the stone-free rates and complications were evaluated in both groups. Results Seven patients in IVU group were excluded from the study. The success rate [95.3%(41/43) in IVU group vs 94.0% (47/50) in control group],stone-free rate [83.7% (36/43)vs 86.0% (43/50)] and complication rate[27.9% (12/43 ) vs 26.0% (13/50)]were similar in two groups (P＞0.05).Conclusions It is not necessary to obtain an IVU for patients who have solitary radiopaque proximal ureteral calculi on plain radiographs with no severe hydronephrosis on uhrasonographie examination before scheduling them for ESWL,thus minimizing the cost,avoiding exposure to contrast medium,and reducing radiation exposure.

Objective To investigate the prevention and mechanism of Extracorporeal shock wave lithotripsy(ESW) induced renal Injury by pre-treating kidneys with low-energy shock waves(LESW).Methods Forty healthy female domestic rabbits were surgically managed to the mono-nephron models and random divided into 4 groups consisting of ten each: Control,LESW,ESW and ESWL plus LESW pretreated groups.LESW group received 100 LESW,ESW group received 1500 standard ESW,and same dose on ESW group except 100 LESW pretreatment in ESW plus LESW pretreated group.The rabbit kidney tissues were obtained 24 hours after ESW.Activity of superoxide dismutase(SOD) and malondialdehyde(MDA) levels in the renal tissue,and the level of N-acetyl-β-D-glucosaminidase(NAG) in urinary were measured.Renal cell apoptosis was detected by TdT-mediated dUTP Nick End Labelling(TUNEL).Results The MDA,the urinary level of NAG and rate of apoptosis in the LESW groups were reduced(P<0.01),and the activity of SOD increased significantly(P<0.05) as compared with ESW group,and these changes in LESW group had no statistics difference compared with the control group(P>0.05).Conclusions LESW pretreatment protocol substantially limits the renal injury that often caused by ESW.LESW may suppress oxidative stress and antagonize the process of renal cellular apoptosis.

Objective To evaluate the clinical efficacy of expulsive therapy using alpha 1-selective adrenoblocker and prostaglandin synthetase inhibitor for distal ureteral stones.Methods 94 patients with distal ureteral stone were randomly divided into study group and control group.In study group,47 cases received tamsulosin 0.2mg daily and dielofenac rectal suppositories 50mg 2 times daily,while watchful waiting without tamsulosin and diclofenac suppository in 47 cases of control group.Observation lasted 2 weeks and also stone expelled as end point.Results No severe adverse reactions related to the drugs were noted and no patients withdrew from the study.The stone-free rate was 91.5%(43/47) of the study group and 25.5% (12/47) of the control group (P＜0.01).A mean stone expelling date of the control group was(8.9±4.3)d and that of the study group was(6.4±3.7)d(P＜0.01).Rates of renoureteral colic recurred in the study group and the control group were 4.3%(2/47) and 48.9%(23/47),respectively (P＜0.01).Conclusion Treatment of distal ureteral stones wlth tamsulosin and dielofenac suppository can increase the stone-free rate,shorten mean stone expelling date,decrease rate of renottreteral colic recurred.

ObjectiveTo explore the characteristics of noncompaction ventricular myocardium under ultrasonic cardiography. Methods8 patients, 1 with non-symptom and other 7 with various cardiac dysfunctions and arrhythmias, accepted ultrasonic cardiography. ResultsNumerous ventricular trabeculae and deep intertrabecular recesses, as well as left ventricular dilatations were found under ultrasonic cardiography.ConclusionNoncompaction ventricular myocardium can be diagnosed with ultrasonic cardiography reliablely.

By a orthogonal experiment, the influence of different ratio of phosphorus and potassium fertilizers on imperatorin, isoimperatorin and psoralen contents and yield of Glehnia littoralis were studied. The results showed that root dry weight and the yield of G. littoralis increased when reasonably applied phosphorus fertilizer combined with potassium fertilizer within a certain range. And the influence of phosphorus fertilizer was greater than that of potassium fertilizer. The optimal value of root dry weight and yield achieved at both P2O5 360 kg x hm(-2), K2O 270 kg x hm(-2) and P2O5 360 kg x hm(-2), K2O 180 kg x hm(-2). The effects of different phosphorus and potassium treatments on the content of imperatorin, isoimperatorin and psoralen in G. littoralis were determined, which shows that the content increased with the moderate increase of phosphorus and potassium. And the effects of phosphorus fertilizer were more significantly. The isoimperatorin content achieved the largest value at P2O5 360 kg x hm(-2), K2O 270 kg x hm(-2), also a larger content of imperatorin and psoralen. The imperatorin content is the largest when applied P2O5 360 kg x hm(-2), K2O 180 kg x hm(-2), and the isoimperatorin content was higher as well. So that the treatment of P2O5 360 kg x hm(-2), K2O 180 kg x hm(-2) are suitable for promote to the agricultural production, which could improve the quality and yield of G. littoralis.
Agriculture ; Apiaceae ; chemistry ; growth & development ; metabolism ; Coumarins ; analysis ; metabolism ; Drugs, Chinese Herbal ; analysis ; metabolism ; Fertilizers ; analysis ; Phosphorus ; analysis ; metabolism ; Potassium ; analysis ; metabolism