Objective:To observe the effect on the apoptosis of breast cancer cell MCF-7 with acupunctural and moxibustional therapy.Methods:Zusanli and Sanyinjiao neiguan in both sides of female Wistar mice were acupunctured and moxibusted.MCF-7 cell was cultirated with the serum.To detecte the cytoxicity with MTT,and detecte the apoptosic rate and Fas expression rate with flow cytometry after 72 hours.Results:The Fas expression was obviously increased upto 57.70% after 72h.The proliferation of MCF-7 was inhibited remarkably.The inhibition rate were 17.55%,24.71% and 31.85% after 12h,24h and 72h.The apoptosic rate were 8.72% and 10.18% after 72h,remarkably higher than the control group(P

Objective To investigate the effect of glycyrrhizin(GL) on the expression of hepatitis B virus e antigen(HBeAg),hepatitis B virus surface antigen(HBsAg),HBV DNA levels and cell proliferation in HepG2.2.15 cell line.Methods HBV DNA level was examined by Real-time PCR.HBsAg and HBeAg levels were examined by ELASA,and cell proliferation was examined by MTT before and after stimulated with GL.The GL groups were compared with the blank control group.Results HBsAg levels in the GL groups were inhibited in dose-dependent manner compared with the blank control group(P

The authors summarized the problems and experiences of the survey on the oral health among people with disability in Zigong city of Sichuan province in 2007,in order to provide some references for the further oral health survey.

0.05).Conclusion:So-fastTM can greatly improve the penetration of PS-ODN into S.mutans and can be used as appropriate delivery system of PS-ODN for S.mutans. No matter what approaches were adopted, the uptake rate reached the maximum at 5 ?mol/L and 2 h-exposure. The penetration can not be enhanced by increasing the PS-ODN concentration and the transformation time.

In the evaluation procedure of medical ethics,it is important to inspect and estimate objectively medical staff's correlated information showed in medical events.Satisfying evaluation of medical ethics can be attributed to the following premises including scientific evaluation regimes,just administration,objective and comprehensive evaluation procedures,just evaluation criteria and archives of medical ethics evaluation.

Document management of medical ethics evaluation includes medical staff's correlated information showed in medical practice.The information conveyed in the documents can be applied in many fields including medical ethics research,medical ethics management,and professional ethics construction,etc.The establishment,application and management of the document are of great importance for medical ethics construction.

Evaluation of medical ethics aims to combine the estimation forms and the administration.Foundation and management of medical ethics evaluation institution will benefit long-term medical ethics mechanism.More attention should be paid to the following aspects as follows.First,much education is in need to elucidate the essence and importance of medical ethics evaluation institution as well as long-term medical ethics mechanism.Second,stringent management is required to have medical ethics mechanism work well.Third,continuous supervision of the faculty will promoting the long-term medical ethics construction.

Multi-media pedagogy is one of the widely interesting audio-visual ways in the field of education at present.This paper analyzed how to phase in this pedagogy in medical ethics course by following several aspects:The place of case pedagogy in medical ethics course, the effect of case pedagogy in medical ethics course and the practice of case pedagogy in medical ethics course.

0.05). In hFOB, the expression of P53 was not detected, and that of P63 was weakly positive. The expression of P53 and P63 in HOS transformed by niekel sulfate were higher than that in normal HOS (P

Objective To explore the pathogenesis-related imprinted genes in osteosarcoma and investigate the role of imprinted genes expression in osteosarcoma pathogenesis.Methods The model of malignant transformed immortalized human osteoblastic cells was established and the malignant transformed cells were collected on day 40,55,70,90.The untreated cells passaged from the normal cells on day 90 served as control.After the total RNA extraction,the finally synthesized biotinylated cDNAs were hybridized to CapitalBio Genechip CAP-F0017.2 of the differentially expressed genes.Two genes were chosen at random to further confirm the array results using the SYBR Green real-time PCR in samples of control,day 40 and day 90. Results By an entrance limit of ≥2.0 or ≤0.5,ten imprinted genes which biological functions are mainly related with cell growth/maintenance and signaling transduction were detectable for notablely differential expression,including down-regulated genes of CD81,GRB10,NDN,MEST and up-regulated genes of H19,MKRN3,SLC22A1L,TSSC3,CDKN1C in the malignant transformation of immortalized human osteoblastic cells.The array results were further confirmed by the real-time RT-PCR.Conclusion Ten imprinted genes were detectable for notablely differential expression.Our results will promote our understanding of the molecular mechanisms of imprinted genes in the course of osteosarcoma pathogenesis.