Brain Injuries ; blood ; Humans ; Hypoglycemia ; pathology ; Infant, Newborn ; Infant, Premature

Objective:To study the therapeutic effect of 2 artificially synthesized fusion peptides on scalded mice. Methods: Two new fusion peptides, M-T508 and M-T?1, were synthesized based on amino acid sequences selected according to the characteristics of scald (including 3 stages: inflammation, infection and repair by cell proliferation). The 2 peptides were applied for second degree scald burn in mice and the healing of the scald burn was observed. The expression ofⅠcollagen, vascular endothelial growth factor (VEGF), proliferating cell nuclear antigen (PCNA), and cytokeratin (CK) was detected by immunohistochemical staining. Serum TNF-? concentration was determined by ELISA in the animals and the blood vessel permeability was analyzed by Evans blue staining. Results: The healing periods of scald burns in the 2 peptide groups were 1 week earlier than that in the control group. Immunohistochemical results showed that the expression of collagenⅠ, VEGF, PCNA and CK was all positive in the 2 peptide groups 10 days after scald and negative in the control group. Examination of blood vessel permeability and serum TNF-? concentration in the M-T?1 and M-T508 groups showed that the 2 fusion peptides had anti-inflammation and anti-infiltration effects in scalded mouse. Conclusion: The synthesized fusion peptide M-T?1 and M-T508 can promote the healing of scald burns in mice.

Objective To study the factors which affect the functional restoration of the elbow for adults with supracondylar or intercondylar fractures of the humerus treated with open reduction and internal fixation. Methods 22 patients with supracondylar or intercondylar fractures of the humerus were treated with open reduction and internal fixation from January, 1999 to December, 2004. They were followed up for an average of 30 months and complete clinical data were kept. The function of the elbow was evaluated with the Mayo elbow performance index. The relationship between the results and the factors —initial time of postoperative rehabilitation, fracture classification, operative approach and interval from injury to operation —were analyzed with cumulative logistic regression model of SAS software V8.2 (SAS institute, Cary, North Carolina). Results Of the 22 cases, 7 were excellent, 9 good, 5 fair and 1 poor. The excellent and good rates were 72.7%. And the odds ratio estimates of the 4 factors were 0.786, 0.299(B/A)0.221(C/A), 1.038, 0.197 respectively. Conclusions The initial time of postoperative rehabilitation, the fracture classification and the interval from injury to operation affect the functional restoration whereas the operative approach does not. Early operation, anatomical reduction, stable fixation and early rehabilitation will help improve the functional restoration.

Objective To investigate the roles of ubiquitin-conjugating enzyme(UBC9)in HSCs activa-tion and liver fibrosis. Methods Western blot was used to analyze the expression of UBC9 under the stimulus of different concentrations of TGF-β1. The effective shRNA-targeting UBC9 gene was synthesized and HCSs were in-stantaneously transfected using lipofectamine method. Non-specific shRNA-transfected group cells and shRNA-tar-geting UBC9-transfected group cells were set up. The mRNA and protein levels of UBC9 were determined with Quantitative Real-Time PCR and Western blot. Western blot also used to examine the expression level of collagenⅠ,α-SMA and P-smad3 after transfection of UBC9 shRNA into HCSs and CCK-8 assay was used to detect cell proliferative capacity after transfection. Results UBC9 expression was significantly up-regulated in TGF-β1-treat-ed HSCs. Knockdown of UBC9 significantly inhibited TGF-β1-induced HSCs proliferation,as well as decreased the expression levels of a-SMA and collagen I. Furthermore ,knockdown of UBC9 attenuated the phosphorylation of Smad3 in the presence of TGF-β1. Conclusions UBC9 may function as a novel regulator to modulate HSC activa-tion,potentially by inhibiting the TGF-β1/Smad3 signaling pathway,which reveals novel mechanistic insights into the anti-fibrotic effect of UBC9.

Objective: To observe the therapeutic effect of peritoneal dialysis on low cardiac output syndrome (LCOS) after surgery in children with congenital heart disease (CHD). Methods: A total of 61 CHD children complicated with LCOS after surgery were selected. Echocardiography was used to measure heart chamber diameter, left ventricular ejection fraction (LVEF) and pulmonary artery pressure etc. before and after peritoneal dialysis; and central venous pressure (CVP), urine volume, blood gas analysis indexes，plasma brain natriuretic peptide (BNP) concentration were measured to evaluate influence of peritoneal dialysis. Results: Compared with before treatment, there were significant increase in urine volume [(203.28±15.81) ml vs. (989.11± 54.72) ml], LVEF [(32.94±2.29)% vs. (51.93±2.79)%]；and significant reduction in pulmonary artery pressure [(46.72±3.84) mmHg vs. (34.82±2.92) mmHg], CVP [(17.27±1.47) cm H2O vs. (10.41±1.01) cm H2O] and plasma BNP level [(5928.71±158.27) ng/ml vs. (3851.83± 128.81) ng/ml], P<0.001 all. Conclusion: Peritoneal dialysis may effectively treat low cardiac output syndrome after surgery in children with congenital heart disease, related with its effect of ultrafiltration and relieve cardiac burden.

BACKGROUND:Present treatments for chronic skin wounds have certain limitations, and adult stem cels play a potentialpart in cutaneous repair and regeneration.
OBJECTIVE:To review effects of stem cels in skin regeneration and wound healing.
METHODS:The first author retrieved CNKI and Medline databases by computer for relevant articles published from 2000 to 2010. Thekeywords were “epidermal stem cels, hair folicle stem cels, stem cels, transplantation, dermal stem cels” in Chinese and in English, respectively. Then totaly 489 papers were obtained after initial survey, and according to the inclusion criteria, 30articles were selected for review.
RESULTS AND CONCLUSION:Epidermal stem cels and other adult stem celshave beenapplied to treat wounds and other skin diseases. Epidermal stem celsarethe crucialcelsource of skin development, repairandremodeling.Epidermal stem celsarealwaysina resting statein vivo.Unless,skin injure or culturein vitro, celdivision and proliferation wilbe significantly fastened. The stability of the epidermis mainly dependson the asymmetric divisionof a subpopulation, in which two daughter celsare produced, including one with characteristics of stem cels, and the other differentiated into transient amplifying cels that wil be differentiated intopost mitotic celsafter a series of cel divisions (3-5 times). Afterwards, those post mitotic celsaredeveloped into terminal differentiation cels onthe basal layer, finaly detachfromthe epidermisasdander. In addition, it is unclear whether epidermal factors are related to apoptosis, migration and differentiation in the process of wound repairandeven under physiological conditions. Therefore, application ofstem celsinwound healing requiresa further discussion.

Humans ; Infant, Newborn ; Jaundice, Neonatal ; diagnosis ; therapy

The article introduces the concepts of medical social work and its performance evaluation,and reviews relevant researches on medical social work home and abroad.In addition,it proposes an operation mechanism and implementation scheme for the evaluation of medical social work in China.

BACKGROUND:Fatigue measurement is conducted while the eyes are gazing at something to accomplish the determination and monitoring of a real-time and non-contact fatigue.OBJECTIVE:To figure out the percentage of eyelid closure (PERCLOS)value for fatigue and deduct the subject's fatigue state.DESIGN:To detect the subject's pupils with a reconstructed eye tracking system ,the video frequency of the pupils is collected, from which the PERCLOS value is worked out by image processing. The PERCLOS value is used to reflect the subject's fatigue state.SETTING:School of Mechanical and Electronic Engineering, Beijing Jiaotong University.MATERIALS :The experiment was conducted in the Ergonomics Lab from September 2003 to November 2004. The equipments used were video transmission cable, frame grab card and computer.METHODS:The video frequency images of pupil module sent from eye tracking system were processed to deduct the closure state of the pupil and figure out the PERCLOS value for the determination of fatigue.MAIN OUTCOME MEASURES :The images collected by image collecting card reflected the state of opening and closure of the pupil.RESULTS:A fatigue determination system which was able to determine effectively the examinee's fatigue state was established.CONCLUSION:This new method expands the application of eye tracking system which can be safely used off-line or on-line.

Objoctive To investigate circulating leveh of soluble vascular cell adhesion molecule-1(sVCAM-1)in the peripheral of intrahepatic cholestasis of pregnancy(ICP).To reveal the pathogenesis of ICP.Methods Maternal scram sVCAM-1 levels were determined in 49 women which 15 eases were normal pregnant women and 34 with ICP.The latter were composed of 14 women with mild,20 with severe ICP.sVCAM-1 was measured by ELISA.Results The levels of serum sVCAM-1 was significantly elevated in women with mild and severe compared with control subjects(P＜0.01),and markedly decreased to normal 5 days after dilively(P＞0.05).The sVCAM-1 level were negatively correlated with weight of neonatal(r=-0.643,P＜0.01).Conclusion Elevated levels of sVCAM-1 indicate that a primary endothelial cell dysfunction play an important role in the ICP.And it influences the neonatal.