Objective To observe changes of the structure and function of microvessels during the formation and development of the rat cerebral glioma with steady-state susceptibility contrast-enhanced MR imaging. Methods A total of 30 bearing-tumor rats were divided into 3 groups (1-week group, 2-week group and 3-week group) and underwent MR examination. Blood volime (BV) and vessel size index (VSI_(MRI)) of peripheral tumor areas, central tumor areas and normal cerebral tissue in the opposite side were measured, and compared with histologic findings. Results With time of bearing-tumor increasing, BV of central tumor area present an up-and-down curve-like change, which related to the host blood vessel regression in tumor central area, the decreasing of blood vessel density and histopathologically obvious necrosis. BV of peripheral tumor areas increased because of active blood angiogenesis in this area, while VSI_(MRI) increased gradually, especially in the central tumor area. There was obviously correlation between the VSI_(MRI) and VSI _(histo) (P<0.01). Conclusion BV and VSI_(MRI) can exactly reflect the morphology and functional information of microcirculation of tumor during the growth and development of gliomas.

Objective To study the impact on the radiation dose,organ doses of eye lens and thyroid and image quality with different scanning modes in head and neck CT scan.Methods The simulation phantom of head and neck was scanned by using fixed scanning condition (120 kV and 200 mAs),as well as the combinations of automatic tube current modulation (CARE Dose 4D),automatic tube voltage modulation technique (CARE kV) and partial angle scanning mode (X-CARE) respectively.Six kinds of scanning modes were adopted,including 120 kV +200 mAs,120 kV + 200 mAs + X-CARE,CARE Dose 4D + 120 kV,CARE Dose 4D + 120 kV + X-CARE,CARE Dose 4D + CARE kV,CARE Dose 4D + CARE kV + X-CARE.Two thermolumineseece dosimeters (TLDs) were exposed at the skin surface positions of eye lens and thyroid,and the values measured with two TLDs were averaged.The CT dose index volume (CTDIvol) and dose length product (DLP) for every scan were recorded,and the contrast to noise ratio (CNR) in eye lens section and thyroid section were measured.Results The crgan doses of lens and thyroid were 19.8 and 26 mGy at 120 kV and 200 mAs,as well as 13.3 and 22.2 mGv at X-CARE mode.Compared with the manual selection of 120 kV,the combination of CARE kV and CARE Dose 4D made the values of CTDIvol drop from 13.1 to 10.1 mGy,the doses of eye lens and thyroid from 16.6 and 20.8 mGy to 23.7 and 19.9 mGy respectively,while the image quality reducedsignificantly.Compared with CARE Dose 4D + 120 kV,the organ doses of eye lens and thyroid were reduced from 20.8 and 23.7 mGy to 9.6 and 15.1 mGy for with additional X-CARE,while CTDIvol dropped from 13.1 to 9.3 mGy.When the combination of CARE Dose 4D + CARE kV + X-CARE was used,CTDIvol and organ doses were reduced to a minimum,when the CNRs of head and neck were also minimized.Conclusions The scanning mode CARE Dose 4D + 120 kV + X-CARE for head and CARE Dose 4D + CARE kV for neck can effectively reduce the radiation dose while keeping good image quality.When requirements for image quality are not high,CARE Dose 4D + CARE kV + X-CARE mode can be selected to reduce the radiation dose significantly.

Objective To explore the of value steady-state susceptibility contrast-enhanced MRI (SSCE-MRI) in assessment of anti-angiogenesis therapy in rat C6 glioma. Methods Seventeen rats with the bearing-tumor were randomly divided into the therapy group (n=8) and the contrast group (n=9). Human-recombination endostatin (10 mg/kg) was injected to animals in therapy group, and saline of the same dose was injected to the contrast group for 7 days. Blood volume (BV) and vessel size index (VSI) were measured of tumors in peripheral, central and normal areas with MR pre- and post-therapy. Results After therapy of endostatin, the tumor volume was obviously different between therapy group and contrast group (t=5.26, P<0.05). BV and VSI_(MRI) of tumor decreased in both peripheral and central areas, particularly in the peripheral area (t=4.14, 3.66, all P<0.01). Conclusion Assessment of therapeutic effect of anti-angiogenesis in C6 glioma is feasible with SSCE-MRI, and it can reflect the changes of BV and VSI_(MRI) pre- and post-therapy.

This paper proposed a rehabilitation training system with electromyography (sEMG) feedback for stroke patients based on ARM embedded system and LabVIEW. The system can achieve real-time acquisition, processing and dualview of multi-channel sEMGs and compute related sEMG parameters including iEMG, RMS, MPF and co-contraction ratio. The system was detected by clinical experiments and related inspection department. The result showed that the system is functional, interactive and in accordance with the relevant standards for medical devices so that it can fully satisfy the clinical demands. In addition, the system can help doctors to master the training state of the patient more effectively in a real-time and quantitative way that is direct to improve the training programs of stroke patients.
Electromyography ; Humans ; Neurofeedback ; Stroke Rehabilitation

Acupuncture Therapy ; methods ; Adult ; Aged ; Female ; Humans ; Male ; Medicine, Chinese Traditional ; Middle Aged ; Tennis Elbow ; therapy

Objective To investigate the effect of intravenous infusion of hyper-oxygenated solution (HOS) on small intestinal ischemia-reperfusion (I/R) injury in rabbits.Methods Twenty-four rabbits of both sexes,weighing 2.5-3.2 kg,were randomly divided into 3 groups ( n =8 each):sham operation group (group S),I/R group,and HOS group.Small intestinal I/R was produced by clamping the superior mesenteric artery (SMA) for 1 h followed by 2 h of reperfusion in I/R and HOS groups,while the SMA was only clamped in group S.HOS was infused intravenously at a rate of 20 ml· kg-1 ·h -1 via the auricular vein starting from the time immediately after clamping the SMA in group HOS and the equal volume of normal saline was infused instead of HOS in group I/R.Blood samples were obtained from the inferior vena cava at 2 h of reperfusion to detect the concentration of serum lactic acid.The animals were then sacrificed and the small intestine was removed for determination of the malondialdehyde (MDA) content,and activities of superoxide dismutase (SOD),catalase (CAT) and glutathione peroxidase (GSH-Px) in intestinal tissues and for microscopic examination.The pathological changes of the intestinal epithelia were observed and the damage.to the mucous membrane was scored.The internal organs were removed and bacterial translocation from gut to the internal organs was observed.Results Compared with group S,the level of MDA and lactic acid,and rate of bacterial translocation were significantly increased,and the activities of SOD,CAT and GSH-Px were significantly decreased in groups I/R and HOS ( P ＜ 0.05).Compared with group I/R,the level of MDA and lactic acid,rate of bacterial translocation,and activities of SOD,CAT and GSH-Px were significantly decreased in group HOS ( P ＜ 0.05).Conclusion Intravenous infusion of HOS can reduce small intestinal I/R injury in rabbits.

Objective To investigate the changes of QT dispersion and heart rate variability in children with ventricular contraction. Methods 50 healthy children who came for medical examinations at Chengdu Women′s and Children′s Central Hospital from January 2012 to January 2014 were selected as control group. 147 children with ventricular contraction admitted in Pediatric Intracardiac Department of Chengdu Women′s and Children′s Central Hospital from January 2012 to January 2014 were divided them into Benign Group (LownⅠ&LownⅡ) and Malignant Group(LownⅢ, LownⅣA, LownⅣB&LownⅤ) according to the results of Lown classification. Benign Group contained 90 cases while Malignant Group contained 57 cases. Difference in QT dispersion and indices of heart rate variability of the three groups were compared. Results There were no statistically differences between control group and benign group in QT dispersion (P > 0.05). QT dispersion of malignant group was much longer than that of control group and the differences had statistically meaning(P<0.001). SDNN(standard deviation of NN intervals) , SDANN (standard deviation of the averages of RR intervals ), SDNN index (index of standard deviation of the averages of NN intervals), rMSSD(root mean square of successive differences) and PNN50(percentage of successive normal sinus RR intervals>50 ms) of malignant group was much shorter than that of control group and benign group and the differences had statistically meaning (P< 0.01). SDNN, SDANN and SDNN index of benign group was shorter than that of control group and the differences had statistically meaning (P<0.05). Conclusions Heart rate variability of children with malignant ventricular contraction becomes significantly shorter and QT dispersion becomes significantly longer. Detection of QT dispersion and heart rate variability can effectively predict the risky degree of ventricular contraction in children.

Objective To investigate the pathological and immunohistochemical characteristics of female bladder ectopic skene glands.Methods A female with bladder tumor was treated in our hospital in May 2013.Preoperative so(n)graphy revealed a 0.9 cm×0.6 cm round solid mass in the bottom of bladder wall.Mass was hypoechoic homogeneous with regular shape,blood flow within the mass was noted.The tumor was treated with transurethral resection.Routine pathological examination suggested bladder ectopic Skene glands.Immunohistochemical stains for prostate specific antigen (PSA),prostate spectific acid phosphatase (PSAP),androgen receptor (AR),estrogen receptor (ER),CD10,cytokeratin 14 (CK14),cytokeratin 18 (CK18),P63,high molecular weight cytokeratin (34βE12),α-methylacyl-CoA racemase (AMACR/p504s) were further performed.Results Routine pathological examination showed prostate glands composed of prostate gland epithelial cells and basal cells in a submucosal location.Immunohistochemical stains showed:PSA-,PSAP +,AR +,ER-,CD10+,CK18 +,CK14-,P63 +,34βE12 +,AMACR-.Conclusions Routine pathological examination combined with immunohistochemical stains such as PSA,PSAP,and others,can be used to diagnose ectopic Skene glands disease.Female bladder ectopic Skene glands is a benign lesion,and the prognosis is good.

Objective To observe the MRI imaging manifestations of the ligaments and soft tissues around the atlanto-axial joint in children with atlanto-axial rotation instability.Methods Assigned into observation group were 50 children with atlantoaxial rotatory displacement who had been treated in our hospital from January 2013 to March 2014.Another 50 healthy children were chosen as a control group who underwent health check-ups during the same period in our hospital.Both groups received MRI examinations of the ligaments and soft tissues around the atlantoaxial joint using the same equipment and methods.MRI manifestations and characteristics of the ligaments and soft tissues around the atlantoaxial joint were analyzed and compared between the 2 groups.Results In the observation group,odontoid gap asymmetry appeared on both sides,with left shift in 32 cases and right shift in 18 cases.The MRI imaging PDWI sequence showed a significant better diagnostic sensitivity than the other sequences (T1WI,T2WI and SPAIR) (P ＜ 0.05).The MRI examinations on all the children with different sequences found 50 cases of degree Ⅰ lesion in the observation group and 4 cases of degree Ⅰ lesion in the control group,and 12 cases of degree Ⅱ lesion in the observation group and none degree Ⅱ lesion in the control group,showing a significant difference between the 2 groups regarding the diagnostic sensitivity of lesions of degrees Ⅰ and Ⅱ (P ＜ 0.05).Conclusions MRI can clearly show the rotation displacement of atlanto-axial joint associated with transverse ligament,alar ligament and lesions of the surrounding soft tissues.MRI has a dcfinite diagnostic value for atlanto-axial rotation displacements in children,especially those caused by transverse ligament tear after trauma.

A 2,4 -dichlorophenol degrading Pseudomonas strain GI241-1 was isolated from a soil sample. The dienelactone hydrolase gene, designated as dcpD which encodes dienelactone hydrolase involved in transforming cis-2-chloro-dienelactone into 2-chloromaleylacetic acid, was cloned from this bacterium strain. The gene cloning strategy was to construct genomic library after location of its neighbouring gene by Southem blot and to screen the aim transformant by dot blotting. Sequencing results showed that length of dcpD is 702bp. The sequence of dcpD and the deduced amino acid are different from the relative sequences registered in the GenBank.