Objective To investigate the effects of electro-acupuncture (EA) on remyelination after sciatic nerve was transected in rats, so as to explore the possible mechanism of EA in treatment of peripheral nerve injury. Methods After transection of rat's sciatic nerve, the nerve regeneration chamber was built,then the rats were given EA stimulation at frequencies of 5 Hz and 100 Hz, respectively, and Solochrome Cyanine staining, HE staining of nerve fibers, immunohistochemical staining and semi-quantitative image analysis technique were employed to observe the remyelination of the nerve fibers and S-100 protein expression of Schwann cells. Results EA could significantly promote remyelination of the nerve fibers, elevate S-100 protein expression of Schwann Cells in repairing process of transected sciatic nerve of rats. The therapeutic effect of 5 Hz EA group was better than that of 100 Hz EA group. Conclusion EA is an effective way for the treatment of peripheral nerve injury. It can promote proliferation of Schwann cells and nerve fiber myelination. The therapeutic effects of EA are frequency-depondent.

Objective To explore the expression of drug transporter organic anion transporting polypeptide2(Oatp2) and P-glycoprotein(P-gp) in brains of chronic epileptic rats induced by lithium-pilocarpine.Methods Chronic epilepsy modles were elicited after status epilepticus(SE) induced by lithium-pilocarpine in adult Wistar rats.The expressions of Oatp2 and P-gp were detected by immuno-histochemistry(SABC) method.Results Positive expression of Oatp2 was predominantly in the plexus epithelial cells and(blood-brain) barrier endothelial cells.Weak staining for Oatp2 was also detected in the neurocyte.In contrast,apparent expression of(P-gp) was seen in brain capillary and neurocyte only.Compared with normal control rats,expression of Oatp2 on the plexus epithelial cells and brain capillary in chronic epileptic rats was significantly decreased(P

Objective To explore the pathogenesis-related imprinted genes in osteosarcoma and investigate the role of imprinted genes expression in osteosarcoma pathogenesis.Methods The model of malignant transformed immortalized human osteoblastic cells was established and the malignant transformed cells were collected on day 40,55,70,90.The untreated cells passaged from the normal cells on day 90 served as control.After the total RNA extraction,the finally synthesized biotinylated cDNAs were hybridized to CapitalBio Genechip CAP-F0017.2 of the differentially expressed genes.Two genes were chosen at random to further confirm the array results using the SYBR Green real-time PCR in samples of control,day 40 and day 90. Results By an entrance limit of ≥2.0 or ≤0.5,ten imprinted genes which biological functions are mainly related with cell growth/maintenance and signaling transduction were detectable for notablely differential expression,including down-regulated genes of CD81,GRB10,NDN,MEST and up-regulated genes of H19,MKRN3,SLC22A1L,TSSC3,CDKN1C in the malignant transformation of immortalized human osteoblastic cells.The array results were further confirmed by the real-time RT-PCR.Conclusion Ten imprinted genes were detectable for notablely differential expression.Our results will promote our understanding of the molecular mechanisms of imprinted genes in the course of osteosarcoma pathogenesis.

Objective To evaluate the rate and types of human papillomavirus (HPV) in vulvar intraepithelial neoplasia (VIN).Methods We detected HPV DNA in 67 lesion tissues collected from 40 VIN patients with PCR based reverse line blot hybridization and DNA sequencing. The PCR using GP5/GP6 primers in HPV L1 region resulted in a 150-bp fragment. When the PCR did not amplify the target DNA,another PCR using SPF1/SPF2 primers was performed to amplify the 65-bp fragment.Results HPV DNA amplified with primers GP5/GP6 was positive in 52.2% (35/67) of the lesions. Of the 32 negative lesions,26 (81.2%) were positive for HPV DNA amplified with SPF1/SPF2 primers. The total positive rate was 91.0% (61/67). Ninety percent of the HPV belonged to high risk types. Sequencing data showed that the genotypes of 31 mono-infection lesions were the same as those of the reverse line blot results,yet the sequences in 4 multi-infection samples could not be determined. Of the 26 SPF PCR products,24 could be sequenced. 80.6% (25/31) patients with multiple lesions displayed the same genotype,suggesting HPV in the different lesions from the same patient was monoclonal.Conclusion The high risk type of HPV is associated with vulvar intraepithelial neoplasia and may play an important role in the development of invasive vulvar carcinoma.

Objective To explore the effects of two air sampling methods including natural precipitation method and impacting method for detecting the bacterial count of indoor air of workplace of cosmetic plants. Methods The diameters of 44 glass bacteria-culture plates for those two sampling methods were measured. The indoor air of workplace of cosmetic plants were sampled by those two sampling methods simultaneously. The natural precipitation method was performed for 5- minute exposure ,the impacting method was performed by MAS-100 airborned bacteria sampler at flow rate of 100 L/min for 30 s,2 min,8 min respectively.All of the data on the bacterial counts of air obtained from various sampling ways were statistically analyzed.The measures for quality control of air sampling progress were put forward also. Results The diameters of 44 glass bacteria-culture plates were 8.39-8.70 cm,which were lower than the standard(9 cm?雪. The bacterial counts of air samples collected by natural precipitation method at the same location showed higher coefficient of variation,higher median,lower qualified rate compared with those by impacting method. The bacterial counts of air decreased,when the impacting sampling method was performed for 8 min continuously with sampling volume of 800 L. Conclusion The impacting method with advantages including mere influence from external environment and better precision could be primarily applied for air sampling in general condition,but it might show lower efficiency of collecting the airborne bacteria during the longer sampling period with higher sampling volume. The natural precipitation method with poor precision was suitable for longer term (8-30 min)air sampling in the relatively static environment with lower air flow and highly cleaned air. The bacterial counts of air obtained from natural precipitation method should be corrected if the diameters of glass bacteria-culture plates weren't meet the requirement of the national standard (9 cm?雪.

Objective To know the air supply quality of central air-conditioning ventilation system in building before and after cleaning.Methods Three sets of central air-conditioning ventilation systems were selected from a building randomly and 3 air supply ways were selected in each system for the determination of inhalable particulate matter(PM10) and microorganisms in air before and after cleaning.Results After cleaning,the unqualified rate of air quality increased from 11.1%(before cleaning) to 77.8%.The mean value of PM10 increased from 0.051 mg/m3(before cleaning) to 0.083 mg/m3,the median of total account of bacteria and fungus increased from 150 cfu/m3 and 13 cfu/m2(before cleaning) to 570 cfu/m3 and 110 cfu/m3 respectively.Conclusion The air supply quality of central air-conditioning ventilation system in building may be damaged by cleaning if the operation is not in the right way.

Objective To understand the distribution character of bacteria in the air of cosmetic workshops and present scientific data for the GMP establishment of cosmetic factories.Methods Of cosmetic factories 11 equipped with air depuration systems (ADS)(type I),13 with no ADS(type II)and 9 producing powder products (such as kermes,face mask,etc.) with no ADS(tyoe III)were chosen,distribution character of bacteria in the air was studied there.The bacteria samplings were conducted before and after operation and in winter and spring,summer and autumn respectively.Results The bacterial count in the three kinds of factories was:type I

The paper first analyzes the current status of and existing problems with the management personnel in domestic third-tier hospitals and expounds from such angles as work motives, knowledge structure and personality the necessary qualities that hospital management personnel should possess. Then it argues on the basis of analyses from such perspectives as methodology, disciplinary system development and macro policy environment that only through appropriate training in hospital management can the current status of the management personnel in domestic hospitals be changed, the professional advancement of the management personnel in the third-tier hospitals be genuinely realized, and the support and impetus for maintaining the competitiveness of domestic third-tier hospitals in the medical market be provided.

Along with the gradual implementation in legislative work of traditional Chinese medicine,the acupuncture education in Australia has rapidly developed.This paper will examine the history of acupuncture in Australia,laws and regulations of acupuncture education in Australia and the Australian acupuncture education system.It will also demonstrate on how the legislation of traditional Chinese medicine has promoted the development of acupuncture education in Australia.

OBJECTIVE: To evaluate the clinical outcomes of medical supporting bone graft following posterior approach and bone cement implantation in the hip joint in treating intertrochanteric fracture.METHODS: A computer-based online search of Science Direct, Ei databases was performed for English articles published between January 1960 and October 2009, with the key words "bone cement, intertrochanteric fracture". In addition, CNKI and CBM were searched for related Chinese articles published between January 1994 and October 2009, with the key words "intertrochanteric fracture, coxa vara, posterior approach of hip joint, bone cement implantation in major screw hole". Moreover,related books were manually searched. Treatment of intertrochanteric fracture, basic and clinical experiment of intertrochanteric fracture treated by bone cement was included.RESULTS: Intertrochanteric fracture treatment includes lateral or anterior approach for dynamic hip plate system, dynamic hip screw, and femoral proximal intramedullary screw internal fixation. Dynamic hip screw is standard internal fixation to treat intertrochanteric fracture, with strong anti-rotation function, and meets the biomechanical requirements. However, screw loosening,extraction and breakage frequently occur. Cancellous bone screw track enhanced by bone cement increases screw retention force,benefits screw compression, enhances bone-screw interface to transfer the stress to screw-bone regions, which significantly increases the anti-flexion and anti-torsion strength of dynamic hip screw and improves fracture stability. The mechanism involves the integral formation of cancellous bone, bone cement and screw by the micro-interlocking of bone trabecula and surrounding cancellous bone.CONCLUSION: Dynamic hip screw filled by bone cement significantly enhances the anti-flexion and anti-torsion strength of internal fixation and improves fracture stability.