Objective To investigate the effects of Puerarin on the expression of hyperphosphorylated-tau (P_ ser404-tau) and choline acetyltransferase (ChAT) in hippocampus of Alzheimer's disease(AD) rats.Methods The rats were divided into sham-operation group, AD group and Puerarin treated group at randomly. The AD rat models were established by injecting amyloid-beta peptide(A?_ 25-35) into right amygdala. The sham-operation rats were injected with trifluoroacetic acid in the same location. The spatial memory function was observed by Y-maze. The protein expression of P_ ser404-tau and ChAT in hippocampus were detected by immunohistochemistry.Results (1)Compared with sham-operation group,the results of maze test were down in AD group and the number of P_ ser404-tau positive cells was significantly higher,while the number of ChAT positive cells was significantly lower (all P

From the beginning of 1980 to the end of 1992, 125 patients with Cushing disease were undergone transsphenoidal microsurgery. 41 patients were operated by selective adenoma resection and 62 patients by radical surgery. 103 cases were available to be analyzed. The results of a long-term follow-up showed: (1) The cure rates were 63. 1% and 48.1% with positive and negative pathological study, respectively. (2) The cure rates were 72.6% and 41. 5% with radical and selective surgery respectively. In cured patients the plasma cortisol level dropped to normal or subnormal within 48 hours after the surgery. However, in uncured patients, it was still above the normal. After operation, 1. 6% of cases (1/60) had secondary hypothyroidism and 62.5 % of cases (30/48) who had abnormal menstruation preoperatively restored normal menstrual cycle. The recurrent rate was 11.4%. Conclusion: (1) The outcome of radical surgery is better than the selective adenoma resection; (2) The pathological study and the analysis of the plasma cortisol level within 48 hours after the surgery may have predictive value for efficacy of the surgery.

Objective To prove the clinical effect of portable auto-haemostat machine on body's limbs. Methods Portable auto-haemostat machine was applied to the indicated patients in their clinical aid treatments course, i.e. the rapid hemostasis on limbs. Results The hemostasis was rapid and accurate with hemostatic pressure in the range from 190 to 230 mmHg. The excellent effect guarantees the diagnosis, observation, bandaging and secure transmission of victims until they were sent to the operation room. Conclusion It is reliable to use portable auto-haemostat machine on body's limbs in clinic works.

Aged ; Alzheimer Disease ; drug therapy ; physiopathology ; Brain ; physiopathology ; Chitosan ; pharmacology ; therapeutic use ; Electroencephalography ; Female ; Humans ; Male ; Phospholipids ; pharmacology ; therapeutic use

BACKGROUND:Genetic studies have shown that there may be association between osteoprotegerin gene G1181C polymorphism and bone mineral density in postmenopausal women, but there are some differences in the conclusions.
OBJECTIVE:To study the association between osteoprotegerin gene G1181C polymorphism and bone mineral density in postmenopausal women through Meta-analysis.
METHODS:The PubMed database, Embase database, CNKI database and Wanfang database were searched for the observational studies about the association between osteoprotegerin gene G1181C polymorphism and bone mineral density in postmenopausal women. The standardized mean difference was considered as the effect indicator and calculated with random or fixed effect models according to the significance of heterogeneity.
RESULTS AND CONCLUSION:A total of 9 articles were included with a total of 8 799 and 9 365 participants for which vertebral and spine bone mineral density had been measured, respectively. The analysis results showed that the bone mineral density of the postmenopausal women with the G al ele of G1181C was significantly lower than that of the postmenopausal women with the C al ele. Similar results were obtained in Caucasian population subgroup analysis. The Caucasian population subgroup analysis showed that the bone mineral density of the postmenopausal women with GG genotype of G1181C was lower than that of the postmenopausal women with GC and CC genotype. The results indicate that there is association between osteoprotegerin gene G1181C polymorphism and bone mineral density in postmenopausal women, and G al ele is a risk factor for lower bone mineral density.

Objective:To investigate the value of B ultrasonography in the early diagnosis of transient synovitis of hip (TSH) in dogs, and provide the valid base and data for the clinic early diagnosis of TSH. Methods:Eighty 2-3 month old dogs were injected 2‰noradrenalin (NA) into the hip joint induced TSH. We observed 5 assessments that included the 99mTC-MDP triphasic imaging, X ray, B ultrasonography, the synovial lfuid and the pathological tissue check in different time. Results:Early course of TSH presented the synovium of joint hemangiectasis, hyperaemia, synovium villus hyperplasia, edema, and joint inflammatory exudation. The ischemia of local blood supply of the femoral head was observed by 99mTC-MDP triphasic imaging. Ultrasonography showed the broadening of the anterior space of the hip, but the X ray showed no valid changes.Conclusion:B ultrasonography can report the early changes of TSH and may be used in the early diagnosis of TSH in children.

Objective:To analyse the stress distribution of the tooth and inlay with the restoration of resin,porcelain,gold alloy,co-balt chromium materials respectively.Methods:3-D finite element models of mandibular first molar with meiso-occlusal (class II) cavity and different inlays were established.Von-mises stress distribution on the tooth and inlay with vertical load and lingual load at 45°were analyzed.Results:After restoration peak stress of high elastic module materials and dental inlays was higher than that of the low elastic module material inlays,however,the restorations of the different elastic module materials had the similar stress distribution trend.The stress under of lateral load(lingual at 45°)to the teeth inlays was significantly higher than that under vertical load.Peak stress concentration of tooth was on the bottom of the cavity near the pulp chamber dentin;inlay peak stress distribution is mainly in its corresponding to the gingival wall.In the tooth tissues stress level of the contact surface of inlay restoration,the strength was as the fol-lowing:Composite resin inlay >ceramics inlay >the gold alloy ceramics inlay >cobalt chromium alloy inlay.The stress level of the inlay of the four kinds of inlay restoration materials was just opponent with the tooth tissues.Conclusion:Gingival wall is the weakest part of meiso-occlusal(class II)cavity inlay restoration,while near the pulp chamber at the bottom of the cavity is the weakest part of the tooth.Among the 4 materials Under the same load condition,composite resin inlay restoration has minimal tooth stress and uniform stress distribution,and can reduce the posibility of odontoschism and microleakage.

DNA methylation is closely related to the genesis and development of glioma.The CpG islands hypermethylation in promoter region of DNA repair genes,cell cycle controlling genes,apoptosis related genes,tumor suppressor genes and invasion related genes are found in glioma.Finding the specific methylation profile and molecular marker of glioma is helpful for the pathology class,early diagnosis and prognostic evalua-tion.Meanwhile,DNA methylation has the characteristic of reversibility,which may provide new ways for the treatment of glioma.

AIM:To evaluate the availability and our experience of intraoperative image-guidance in endoscopic nasocular operation.METHODS:Seven cases of endoscopic nasal surgery with intraoperative image-guidance were retropectively reviewed,including 3 cases of optic nerve injury;3 cases of foreign object of optic behind the eyeball;1 case of retrobulbar tumor(angeioma).RESULTS:The preoperative preparatory time would take 15 minutes,including coordination,head holder localization,conventional instrument registration.In our cases,the localization accuracy between 3-D image landmarks of navigation system and actual anatomical landmarks was less than 1.3mm.The optic nerve and other anatomical points could be orientated accurately in intraoperative procedures.No complication occurred.CONCLUSION:Nasal endoscope combined with imageguidance systems provides accurate anatomical localization of anterior skull base with enlarged operation field.It is possible for surgeons to observe important anatomical structures during endoscopic surgery.It could increase the effectiveness and decrease surgical complications,especially in complicated cases.

Objective To systematically evaluate the effects of albumin compared with crystalloids on fluid resuscitation for adults with severe sepsis and septic shock.Methods The six databases as follows:Cochrane Library,Pubmed,Embase,CNKI,VIP and Wanfang,were retrieved by computer,from their inception to November,2015.Randomized controlled trials(RCT) in the treatment of adult patients with severe sepsis and septic shock were included for quality assessment and Meta-analysis was performed by using RevMan5.3 software.Results A total of 4 RCTs involving 3 862 participants were included.No differences were found between albumin and crystalloids resuscitation with respect to 28-day mortality[RR=0.95,95%CI(0.86,1.05),P=0.33],90-day mortality[RR=0.95,95%CI(0.86,1.05),P=0.33],duration of stay in ICU,duration of hospital stay,duration of mechanical ventilation and renal replacement therapy.Conclusion In that meta-analysis,the effects of fluid resuscitation with albumin or crystalloids were not significantly different in adults with severe sepsis and septic shock.