1.Three dimensional CT for measuring mandibles morphology in 54 normal Chinese adults
Quan CHEN ; Chuanbin GUO ; Tao GAO
Journal of Peking University(Health Sciences) 2015;(1):113-119
Objective:To establish a method of morphologic measurement of the mandibles and obtain the average values of three dimensional morphologic measurements of the mandibles .Methods: A me-thod of morphologic measurement of the mandibles was established firstly .The three dimensional CT data of 54 normal adult skulls were measured by this method .The data were analyzed by SPSS 13.0 statistical software.Results:In the study, 84 groups of mean values and standard deviations of the length , width, height, depth, thickness and angle of the mandibular contour in males and females were obtained .There were significant differences between the male and the female in the 66 of the 84 groups data , while the 16 of the 84 groups data had no significant differences and distributed symmetry on both sides of the mandi -bles .No correlation was found in the mandibular contour data in length , width , height and depth .Con-clusion:The characteristics of adult mandibular contours are different between males and females , indi-cating that each individual has its own morphologic features .
3.A study on posterior microendoscopic discectomy to treat lumbar disc herniation and lumbar spinal stenosis
Tao GUO ; Yong DENG ; Dengjun CHEN
Chinese Journal of Minimally Invasive Surgery 2001;0(06):-
Objective To evaluate the application value of Microendoscopic Discectomy (MED) in the treatment of lumbar disc herniation and lumbar spinal stenosis. Methods 188 cases of lumbar disc herniation and lumbar spinal stenosis were treated by MED, 38 cases of them performed lateral recess decompression. Results The mean follow-up period was 6.9 months. excellent and good therapeutic result rate was 97.4% (Macnab's standard). Conclusions MED in the treatment of lumbar disc herniation and lumbar spinal stenosis has short-term satisfactory therapeutic efficacy.
4.Reflection on the construction of public health management teachers troop
Yu CHEN ; Yuting GUO ; Tao LIU
Chinese Journal of Medical Education Research 2003;0(02):-
The specialized teacher troop is the discipline construction,the development key,the teaching leading force and the personnel training quality guarantee. At present stage the quantity and quality of the specialized public health management teacher staff in our school have gradually become the bottleneck for the career development. Only by sharpening teachers' scientific research ability unceasingly, enhances teachers ' individual accomplishment and the teacher troop's overall quality,improving their work quality,can we adapt to the career devel-opment under the new situation.
5.Effects of magnetic stimulation on proliferation and differentiation of endogenous neural stem cells/progenitor cells after spinal cord injury in rats
Tao XU ; Fengjing GUO ; Anmin CHEN ; Xiaolin HUANG ; Tiecheng GUO
Chinese Journal of Physical Medicine and Rehabilitation 2010;32(1):2-5
Objective To investigate the effects of magnetic stimulation (MS) on the proliferation and differentiation of endogenous neural stem cells (NSCs)/progenitor cells after spinal cord injury (SCI) in rats. Methods Forty-six Wistar rats were used, of which 40 were used to make an animal model of spinal cord injury (SCI) by administering a 10 g x 12.5 cm impact at the T8 level. The other 6 served as the normal controls. The SCI model rats were evenly divided into a magnetic stimulation (MS) group ( n = 20) and a control group ( n = 20). The rats in the MS group received 0.5 Hz and 1.44 T magnetic stimulation 24 h post injury, then 30 pulses per day for 7 days. The rats in the other groups were not exposed to MS. The scale of Basso, Beatti and Bresnahan (BBB) was used to assess hindlimb neurological function. Rats were sacrificed at the 24th hour, and at the 1st, 4th and 8th weeks after SCI. The ratio of nestin to microtubule associated protein 2 (MAP2)/nestin in the cells of the spinal cord was determined by immunofluorescence. Results The BBB scores in the MS group were signifi-cantly higher than those of the control group at 1, 4 and 8 weeks post SCI. Nestin and the MAP2/nestin ratios were mild in the normal spinal cords, but increased after SCI. They were higher in the MS group than that in the control groups at all time points. Conclusions MS can promote nestin expression in the spinal cord after SCI and facili-tate neural differentiation.
7.Advantage of separate bolus injection technique in CT urography
Tao CHEN ; Guo WU ; Xiaorong CHEN ; Nian ZHAO ; Yong ZHANG
Chinese Journal of Postgraduates of Medicine 2013;(12):18-20
Objective To investigate the advantage of separate bolus injection technique in CT urography (CTU) improving the display of the whole urinary tract.Methods Sixty cases of CTU examination,were divided into observation group and control group by random digits table with 30 cases each,observation group used separate bolus injection technique and control group used single bolus injection technique.The scanning included routine scanning,cortical phase,medullary phase and lag phase.Reconstruction of lag phase displayed the whole urinary tract.Then the image quality was compared between two groups.Results The whole urinary tract showed excellent in 12 cases (40.0%,12/30),good in 17 cases (56.7%,17/30),normal in 1 case (3.3%,1/30) in control group,which showed excellent in 23 cases (76.7%,23/30),good in 7 cases (23.3%,7/30) in observation group,there was significant difference in excellent rate between two groups (P < 0.01).The CT value of starting of ureter was (238.6 ± 82.5) HU,middle-lower ureter was (245.9 ± 112.3) HU in control group and (239.0 ± 93.8),(235.3 ± 74.6) HU in observation group,there was no significant difference between two groups (P >0.05).There was no difference in developing of urolithiasis between two bolus injection techniques.Conclusion The application of separate bolus injection technique in CTU examination can reduce the dose of the first contrast material and contrast material reaction,and receive high-quality image of the whole urinary tract.
8.The effects of trichloroethylene on cardiac differentiation in human embryonic stem cells and its mechanisms.
Dan WANG ; Tao CHEN ; Guo-qing WANG ; Yan JANG
Chinese Journal of Applied Physiology 2015;31(3):216-224
OBJECTIVETo explore the effects of trichloroethylene (TCE) on cardiac developmental differentiation in human embryonic stem cells.
METHODSIn this study, based on the human embryonic stem cells in vitro cardiac differentiation assay, we investigated the potential effect of TCE exposure on the cardiac toxicity in embryo development. Human embryonic stem cells were treated with TCE at different concentrations of 100 ppb, 1 ppm, and 10 ppm and dimethyl sulfoxide(DMSO) treated as control. The MTT assay was performed to examine the cytoplasmic toxicity of TCE exposure. The beating percentages were recorded and the expression of cardiac specific gene was evaluated by PCR or flow cytometry. Also, real time PCR was performed to verify the micro array analysis on the expression level changes of genes which were involved in the Ca2+ signal pathways.
RESULTSCompared with the control group, there was no significant difference in cell viability when cells were treated with TCE at the concentrations of 100 ppb, 1 ppm, and 10 ppm. However, TCE could inhibit the expression of cTnT protein in a concentration-dependant manner. And the most interestingly, TCE significantly inhibited the cardiac differentiation characterized by the decrease beating percentages. Genes involved in Ca2+ signaling pathway were severely disrupted by TCE.
CONCLUSIONTCE inhibited the cardiac specific differentiation of human embryonic stem cell and at the meanwhile the genes responsible for Ca2+ signaling pathway were severely disrupted, which could contribute the severe effects of TCE cardiotoxicity.
Calcium Signaling ; Cell Differentiation ; Cells, Cultured ; Embryonic Development ; Embryonic Stem Cells ; cytology ; drug effects ; Heart ; embryology ; Humans ; Trichloroethylene ; toxicity
9.Scheme Design and Results Analysis of Ground Bond Proficiency Testing.
Tao CHEN ; Yichuan ZHANG ; Dawei LU ; Baosheng GUO ; Bingzhen WEI
Chinese Journal of Medical Instrumentation 2015;39(6):454-456
Grounding impedance measurement is a traditional proficiency testing programs, 2014 proficiency testing program on the basis of original ability to verify, combined with actual detection need, innovation introduced two verification point of the power input socket and metal plane testing. This paper analyzes and discusses the results of the ability verification in 2014, and puts forward the points of attention and the recommended method of metal plane test.
Laboratories
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standards
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Laboratory Proficiency Testing
10.Strengthening Management of Reasonable Application of Antibacterial Drug
Wenguang CHEN ; Jinghua JIANG ; Zebao ZHANG ; Ying TAO ; Yachun GUO
Chinese Journal of Nosocomiology 1994;0(04):-
OBJECTIVE To achieve the best treatment effect by the effective management to control abusing the clinical antibacterial drug. METHODS The management organization and system on antibiotics reasonable application were established.It also formulated an standard to examine the reasonable antibiotics application.It construced the information system of the antibiotics reasonable application management,to carry on the real-time monitoring to the clinical antibiotics application situation. RESULTS The antibiotics utilization ratio was from original 78% lowered to 65% and the antibiotics use hastened more reasonably. CONCLUSIONS The standardized management of antibiotics use is realized and the medical quality is improved.