Animals ; Catechin ; analogs & derivatives ; pharmacology ; Disease Models, Animal ; Fatigue ; metabolism ; physiopathology ; Learning ; drug effects ; Male ; Memory ; drug effects ; Rats ; Rats, Sprague-Dawley ; Receptors, N-Methyl-D-Aspartate ; metabolism

ObjectiveTo explore the mechanism and prevention for the hypertension complicated by the retroperinoneal paraganglioma operation.MethodsBlood pressures were monitored during operations on 15 patients with retroperinoneal paraganglioma. Also,the reason and the prevention for the changed blood pressure were studied emphatically during operation.ResultsThe elevated blood pressure of 12 patients was detected during operation,and all dropped to a varied degree after operation,with 2 of them below the normal value.ConclusionThe retroperinoneal paraganglioma operation was liable to cause blood pressure elevated,which was related to the anesthesia induction and the operation stimulation of tumor. After the excision of the tumor,blood pressure of most of the patients dropped to some degree. Therefore,during the retroperinoneal paraganglioma operation,the blood pressure should be monitored closely,and the short-acting antihypertensives should be applied on time. Meantime,occurrence of hypotension was also considered after the excision of the tumor.

BACKGROUND:Previous research has demonstrated that preoperative hormone therapy relieved clinicaI and pathological stages of prostatic carcinoma patients,reduced positive ratio of incisaI margin:however,it did not increase disease-free surviva J rate.Worth of preoperative hormone therapy for prostatic carcinoma remains unclear.OBJECTIVE:To assess the role of preoperative hormone therapy in localised prostate cancer.METHODS:Articles were searched from PubMed,Embase.Cochrane Library(No.4,2009),Chinese biomedicaf literature database,Chinese Scientific Journals full_text database,and Chinese Journal full-text database(published before October,2009).Randomized controlled trials which were diagnosed as Iocalised prostate cancer using pathology and cytology were included The sex and nationality were not limited.and patients did not have severe heart and lung diseases Prostatic carcinoma which occurred Later or repeatedly was excluded.Otherwise.randomized controlled trails which were coincidence with the inclusion criteria were also included.Review Manager 5.0 published by Cochrane was used for stafistical analysis.Overall survival rate.disease-free survivaI rate,positive surgical margin rate,positive lymph node rate.and seminal vesicle invasion rate were evaluated.RESULTS AND CONCLUSlON:Six randomized controlled trials totaling 1 027 padicipants were included.The result of meta analysis showed that there was no significant difference in overall surviva J rate[RR=0.94,95%C,(0.86,1.02)],disease-free survival rate[RR=1.02,95%CI(0.89,1.17)],positive lymph node rate IRR=0.86,95%CI(0.47,1.57)],and seminal vesicle invasion rate[RR=1.09,95%CI(0.74,1.59)]between single prostatectomy and preoperative hormone therapy plus prostatectomy,while there was significant difference in positive surgical margin rate[RR=0.46,95%CI(0.32,0.66)]This suggested that preoperative hormonal therapy prior to prostatectomy did not improve survival rate,positive lymph nodes rate and seminaI vesicle invasion rate:however.there was a significant reduction in the positive surgicaI margin rate.

Objective The aim of our study is to establish and characterize the animal model for au- toimmune myositis.Methods Fifty male SD rats were randomly divided into 2 groups:model group(n=40) and control group(n=10).The model group rats were immunized with muscle homogenate every week for 5 weeks and received an injection of 2?g pertussis toxin at the first and second week.As controls,10 SD rats were injected with an equal volume of normal saline.Tissue specimens from limb skeletal muscles were ob- tained at 1,2,3,4,5 weeks after injection.At the same time,the blood samples were collected,and the level of CPK was measured.Results The model group had significantly elevated serum CPK levels.There were multiple inflammatory lesions in the skeletal muscles.Local degeneration and necrosis of muscle fibers with disappeared transverse striation,mononuelear cell infiltration in the interstitial could be observed.The patho- logic grade was mainly 2a.The infiltrating mononuclear cells were predominantly CD8~+T cells that mainly lo- cated in the endnmysium.MHC classⅠantigen expression on muscle fiber membranes in the model group was upregulated.Conclusion The experimental autoimmune myositis induced by syngeneic skeletal muscle ho- mogenate in SD rat is pathologically similar to human myositis.It can be used as a good model for human myositis and provides the basis for the etiopathology and therapeutical studies.

Objective To explore the mechanism that gold nanorods trigger apoptosis in cancer cells.Methods Gold nanorods was synthesized by gold seed growing method, and its characterization was detected; gold nanorods on cell proliferation-toxicity were evaluated by CCK-8 Kit and apoptosis were detected by flow; mitochondrial membrane potential were tested by JC-1 and activation of Caspase 9 and Caspase 3 were detected by western blot. Results The results found that gold nanorods had nontoxic to normal cells, but highly toxic to tumor cells; and with the increasing of gold nanorods’ working time, the percentage of apoptotic cancer cells was increasing; in addition to, normal cells’ mitochondrial membrane potential did not change, but cancer cells had a significant reduction in mitochondrial membrane potential.Conclusion This study proves that gold nanorods induce apoptosis through the mitochondrial apoptosis pathway.

With more and more attention and investment on acupuncture scientific researches, considerable outcomes and achievements has been acquired, but the shortcoming of low transformation rate of acupuncture research achievements is gradually exposed. Nowadays there is no related report on this problem, so based on achievement translational research in other areas and practical situation of acupuncture, the existing problems and solutions are analyzed. As a result, the existing problems include (1) the research content is mainly basic research and clinical research but less acupuncture device research, leading to limited transformation efficiency; (2) the evaluation system and transformation pattern are still needed to be perfect. The solutions are (1) to properly evaluate the research achievements of acupuncture, (2) to advocate the concept and method of translational medicine, (3) to reform the policy and system, and (4) to establish valid platforms covering research, outcomes and transformation.
Acupuncture ; economics ; legislation & jurisprudence ; manpower ; Biomedical Research ; Biotechnology ; economics ; legislation & jurisprudence ; manpower ; China ; Humans ; Technology Transfer

OBJECTIVETo evaluate the local recurrence and 5-year survival of proximal fibular osteosarcoma.

METHODSFrom October 1997 to July 2005, 12 patients with proximal fibular osteosarcoma were treated. There were 9 males and 3 females. The mean age at diagnosis was 16 (9-23) years old. Histopathological diagnosis were obtained by trut-cut biopsy, Enneking surgical stages of 12 lesions were as follows: 11 grade II B and 1 grade III. Induction chemotherapy was applied to all patients except one who left the hospital after the biopsy. Of 11 patients, 8 were performed Malawer type I resection, and 3 type II. All patients continued to get postoperative chemotherapy.

RESULTSRegular follow-up was applied. Only 1 patient was found local recurrence one year after operation and received re-resection, the others did not. Four patients died of lung metastases, including the one who did not get treated, one stage III and two II B patients. The range of follow up was 6 to 117 months. The evaluated 5 year disease-free survival of 11 patients who got treated was 72.7% by using Kaplan Meier survival analysis.

CONCLUSIONSThe local recurrence is low, and 5 year disease-free survival is acceptable, in accordance with present point that marginal resection may not impact survival for fibular osteosarcoma. No knee instability is found in this group after great care is taken for proper reconstruction of lateral collateral ligament and reinsertion of biceps femoris tendon on the lateral condyle of tibia.

Adolescent ; Adult ; Bone Neoplasms ; surgery ; Child ; Female ; Fibula ; Follow-Up Studies ; Humans ; Male ; Neoplasm Recurrence, Local ; Osteosarcoma ; surgery ; Survival Analysis

Objective To comparative study of the immunomodulatory effects of Hedysarum polybotrys polysaccharides (HPS) etc.four species TCM polysaccharides on immunocompromised mice model made by cyclophosphamide.Methods Low-immunity model mice made by cyclophosphamide 40 mg · kg-1.The KM mice were divided into seven group:normal,model,control,experimental-HPS,experimental-Astragalus polysaccharides (ASPS),experimental-Codonopsis pilosula polysaccharides (CPS) and experimental-Angelica polysaccharides (ANPS) groups randomly.The control group was administrated Shenqi particle 5 g · kg-1.The other tested groups were administrated the corresponding test samples 2 g · kg-1.The normal and model group were administrated the same quantity of normal saline.Give the medicine 1 time each day for 15 days.The visceral index was counted by weighted.The peritoneal macrophage phagocytic count by intraperitoneal injection 5％ chicken red blood-cell were meaeured (OD value).The serum hemolysin OD through delayed type hypersensitivity maded by 50％ dinitrochlorobenzene.The reticuloendothelial system phagocytic count were meaeured by caudal vein injection carbon inkl.Results Compared with the normal,the difference of all indexs of model were statistically significant(all P ＜ 0.01).After dosing,the thymus indexs of model,control,experimental-HPS,experimental-ASPS,experimental-CPS and experimental-ANPS groups were 2.55 ± 0.88,1.04 ± 0.74,2.00 ± 0.89,2.21 ±0.95,1.92 ±0.43,1.67 ±0.41.Compared with the model,difference of experimental-HPS,experimental-ASPS and experimental-CPS were statistically significant (all P ＜ 0.05).The phagocytic counts of mouse peritoneal macrophages of the six groups were 1.31 ±0.18,0.51 ±0.12,1.12 ±O.13,1.19 ±0.09,0.88 ±0.25,0.72 ±0.07.Compared with the model,difference of four species polysaccharides were all statistically significant (all P ＜ 0.01).Compared with the experimental-ANPS,difference of experimental-HPS and experimental-ASPS were statistically significant(P ＜ 0.05,P ＜ 0.01).The phagocytic coefficient of reticuloendothelial system phagocytosis of the six groups were 6.60 ± 0.94,0.99 ±0.83,0.32 ± 1.48,7.28 ± 2.13,5.29 ± 1.97,5.93 ± 1.75.Compared with the model,difference of experimental-HPS and experimental-ASPS were statistically significant (P ＜ 0.05 or P ＜ 0.01).The delayed type hypersensitivity OD of the six groups were 0.53 ± 0.16,0.23 ± 0.09,0.36 ± 0.09,0.38 ± 0.12,0.29 ± 0.10,0.33 ± 0.11.The serum hemolysin OD of the six groups were 0.60 ± 0.12,0.43 ± 0.10,0.53 ± 0.08,0.54 ± 0.11,0.51 ± 0.11,0.52 ± 0.09.Compared with the model,difference of experimental-HPS,experimental-ASPS and experimental-ANPS were statistically significant (P ＜ 0.05 or P ＜ 0.01).Conclusion These four kinds of polysaccharides have the function of enhancing nonspecific immunity function,specific humoral immunity and cellular immune function in mice.HPS and ASPS were more comprehensive and significant for the regulation of immune indexes than CPS and ANPS.

With an objective to provide an experimental basis for scientific officinal of Schisandrae Sphenantherae Fructus, this research uses UPLC-TQ/MS method to analyze 7 different kinds of lignan in 70 batches of Schisandra sphenantherae Fructus samples from 9 regions. The results showed that in the area south of Qinling mountains, Schisandrae sphenantherae Fructus from Zhashui county and Shanyang county of Shangluo mainly contained schisantherin A and deoxyschizandrin. However, Schisandrae sphenantherae Fructus from Mei county of Baoji, Shiquan county and Ningshan county of Ankang, and Lueyang county of Hanzhong, mainly contained anwuligan. Samples from Ningshan county also consists relatively high level of deoxyschizandrin. In the central area of Qinling mountains and the Daba mountains, Schisandrae Sphenantherae Fructus from Nanzheng county of Hanzhong mainly contained schisanhenol and deoxyschizandrin. In conclusion, the kinds and level of lignan differ significantly in Schisandrae sphenantherae Fructus produced in different regions. In practical application, Schisandrae Sphenantherae Fructus produced in different regions should be distinguished and differently applied based on their main effective components corresponding to different diseases, which can lead to the best clinical use.
China ; Drugs, Chinese Herbal ; chemistry ; Fruit ; chemistry ; Lignans ; chemistry ; Quality Control ; Schisandra ; chemistry

Objective To analyze the effect of minimally invasive puncture combined with continuous lumbar subarachnoid drainage in the treatment of thalamic expanding hematoma. Methods Seventy-eight patients with thalamic expanding hematoma admitted in our department from 2004 to 2008 underwent minimally invasive puncture combined with continuous lumbar subarachnoid drainage according to the size of intracranial hematoma and the involvement of the cerebral ventricles. Results Of the 78 cases, death occurred in 10 cases, with a mortality rate of 12.8%. The other 68 (87.2%) patients exhibited postoperative recovery of different degrees. Follow-up of the 68 patients for over 6 months after the operation showed that 30 patients had grade 1 Activity of Daily Living (ADL) with independence in daily living, 25 had grade 2 ADL (partially independent in daily living), 19 had grade 3 ADL (capable of assisted walking), 3 had grade 4 ADL (bedridden but conscious), and one patient had grade 5 ADL (in persistent vegetative state). Conclusion This combined treatment with individualized operations reduces the surgical trauma to the minimum and lowers the cost for effective elimination of the hematoma. This procedure also effectively lowers the intracranial pressures and promotes the neurological recovery of the patients with thalamic expanding hematoma.