Aim To investigate the effects of EDT on anoxia and ischemic injury in cultured PC12 cells. Methods Cultured PC12 cells were treated with 1 mmol?L -1 Na 2S 2O 4 and 20 mmol?L -1 NaCN in combination with glucose deprivation. The protective effects of EDT on these two models were evaluated by lactate dehydrogenate (LDH) efflux assay and colormetric MTT assay.ResultsEDT, within the range of 10 -8～ 10 -6 mol?L -1, significantly antagonized LDH efflux induced by two models and increased the optical density at 570 nm tested by colorimetric MTT assay in concentration-dependent manner. 10 -6 mol?L -1 EDT might time-dependently inhibit two injuries and reach maximal level at 48 h. Conclusion EDT can protect PC12 cells from anoxia and ischemic injury.

AlM:To evaluate the value of color Doppler ultrasound in the diagnosis of traumatic retinal detachment.
METHODS: All 50 cases ( 56 eyes ) of suspicious traumatic retinal detachment from January to June in 2013 were enrolled in this study. All first received the two-dimensional ultrasound, then the color Doppler ultrasound, finally, these results were compared with the results of surgery.
RESULTS: All 56 eyes were observed intravitreal abnormal spots by the two-dimensional ultrasound, and 39 eyes were observed color blood signal by the color Doppler ultrasound. Forty eyes were approved retinal detachment by surgery.
CONCLUSlON: lt is better to display the intravitreal abnormal spots and color blood signal by the color Doppler ultrasound than by the two - dimensional ultrasound, so there is important clinical significance of the color Doppler ultrasound in the diagnosis of traumatic retinal detachment.

Fungal enteritis was rarely reported.A case of fungal enteritis manifestation with jejunum multiple ulcers and obstruction was treated by Department of Gastroenterology,Third Xiangya Hospital,Central South University.After antifungal treatment,the clinical symptoms were relieved,and the ulcers in jejunal and upper gastrointestinal tract were healed completely.Clinical manifestation for small fungal enteritis is special,and the small intestine ulcer is easily to be misdiagnosed.It is helpful to prevent the misdiagnose for small fungal enteritis if we can tell the clinical features for this disease.

Adult ; Female ; Granuloma, Respiratory Tract ; pathology ; Humans ; Lung, Hyperlucent ; pathology ; Male ; Middle Aged

Objective To find out the best orientation and section for diagnosing rheumatic heart disease using multiplane transesophageal echocardiography (TEE). Methods The visible heart was compared with the images by multiplane TEE for the establishment of the best orientation and section for multiplane TEE for the diagnosis of rheumatic heart disease. Results The best orientation and section of mitral valve detected by multiplane TEE were the four chamber view at 0? orientation at the end part of esophagus. The best orientation and section of aorta valve were short axis section of the heart at 45? orientation and long axis section of the heart at 135? orientation at the middle part of the esophagus. The best orientation and section were the short axis section at 45? orientation and the oblique short axis section at 0? orientation of the aorta valve at the middle and the upper part of esophagus. Conclusion The best orientation and section of TEE based on the study of the visible heart are helpful to simplify the operation procedure of multiplane TEE and to shorten the examining time.

Objective To investigate the role and mechanism of resveratrol in a rat model of complex region-al pain syndrome type Ⅰ(CRPS1). Methods 50 male SD rats were divided into control group(group A),sham operation group(group B),model group 1(group C),model group 2(group D),and model group 3(group E). Groups A,B and C received 5%DMSO;group D received ISO-1 of 1 mg/(kg·d);and group E received resveratrol of 20 mg/(kg · d)for 14 days. Pain behaviors were assessed on days 0,7,and 14. Serum levels of MIF and TNF-αwere detected by ELISA. MIF ,total ERK1/2 and p-ERK1/2 in sciatic nerve were detected by Western blot. Re-sults On days 7 and 14 after treatment,resveratrol injection,similar to ISO-1,significantly improved the pain threshold;serum levels of MIF and TNF-α were significantly decreased;expressions of MIF ,total ERK1/2 and p-ERK1/2 in sciatic nerve were also decreased significantly in group E,which were significantly lower than group C (P < 0.05). Conclusions Resveratrol can significantly improve pain threshold ,decrease expressions of MIF and p-ERK1/2 in a rat model of CRPS1,which might be involved in the inhibition of ERK signaling pathway.

Objective To evaluate the feasibility and effects of endoscopic thyroidectomy(ETE) for papillary thyroid carcinoma(PTC).Methods A total of 137 patients with PTC were enrolled from January 2013 to January 2016.The patients were divided into two groups depended on different surgical approaches.The patients'' characteristics,such as age,gender,pain,intraoperative blood loss,hospitalization,central lymph node metastasis etc were retrospectively analyzed in the two groups.Results In the study,78 patients performed ETE,and 58 performed COT.There was one case converted to open surgery from ETE due to intraoperative bleeding.The clinicopathological characteristics and operative complications between the two groups had no significant differences,such as gender,time of drainage,postoperative hospitalized days,positive lymph node metastasis,injury of recurrent laryngeal nerve,permanent injury of parathyroid glands.The age of patients was younger in ETE(t=-4.299,P=0.000).The drainage content(t=9.539,P=0.000),intraoperative blood loss(t=2.862,P=0.005) and subcutaneous ecchymosis (χ2=13.482,P=0.000) were found more in the ETE.ETE required a longer operative time(t=8.162,P=0.000).However,ETE provided better cosmetic outcomes than COT [(9.4±0.5)points vs.(5.4±1.0)points,t=30.142,P=0.000].No clinical recurrence and metastasis occurred for at least one-year follow-up.Conclusion With excellent cosmetic results,ETE is a feasible and safe operation for patients with PTC without metastasis to lateral cervical lymph nodes.

Objective To analyse effect of different doses of parecoxib on perioperative cellular factors and the maintenance dose of propofol in patients with laparoscopic cervical cancer surgery.Methods Collected 60 patients who were diagnosed with cervical cancer in our hospital from June 2014 to June 2015.All patients underwent laparoscopic surgery,according to the injection of parecoxib different amount before induction of anesthesia divided into control group,low dose group and high dose group,20 cases in each group,respectively before anesthesia, postoperative, postoperative 12 h and postoperative 24 h, of all patients with serum IL-6, IL-12 and TNF-a level,intraoperative propofol maintenance dose,and postoperative analgesia were detected.ResuIts The levels of serum IL-6 and TNF-αin three groups showed that:high dose group low dose group>control group (P<0.05).The maintenance dose of propofol in the three groups showed that:high dose group

Objective To evaluate the value of 320 row CT upper abdominal computed tomography angiography (CTA) in the follow up of transjugular intrahepatic portosystemic shunt(TIPSS) .Methods 21 cases after TIPSS therapy underwent upper ab-dominal CTA .Post-processing images ,including MPR ,VR ,MIP ,and CPR ,were used to determine the position and patency of the stent .The best images of the portal veins were obtained .Results Among the 21 cases ,CPR image clearly demonstrated endovascu-lar stent thrombosis in 3 cases(14 .29% ) with stent occlusion and 1 case(4 .76% ) with stent stenosis ;there were no stent stenosis or occlusion in 17 cases(80 .95% ) ,their CPR image showed the contrast agent was filled completely in the stent and no low-density thrombosis was found .The best phase of the portal vein was 45 s .Conclusion 320 row CT upper abdominal CTA can clearly dis-play the stent position and patency situation in patients ,therefore ,should be regarded as the first choice for the follow-up of patients after TIPSS therapy .

The rate of complete surgical resection is still low in hilar cholangiocarcinoma,which greatly affects the curative effect.Radiotherapy,one kind of treatment for tumors,has not been widely adopted in the past years.In recent years,with the development of radiotherapy technology,research of treating hilar cholangiocarcinoma through radiotherapy has become a spot.Many studies have shown that radiotherapy,as an adjuvant therapy for surgery and non-surgical treatment,can be the major means for treatment,and it could bring benefit for patients' survival extension and improvement of life quality.