Objective To explore the effect of Mudan granule combined with zinc sulfate on ankle brachial index in the treatment of diabetic foot dorsal artery sclerosis and its efficacy.Methods 66 cases of patients with diabetic foot dorsal artery sclerosis in this study were selected and divided into 2 group, 33 cases in each group.Control group received mecobalamin injection and zinc sulfate tablet and treatment group received Mudan granule on the basis of control gorup.7 days for a course of treatment and patients received continuous treatment of 4 courses.Clinical efficacy, blood glucose, blood lipids, blood rheology, blood flow dynamics of the dorsal artery of the foot, ankle brachial index, and adverse reactions were compared after the treatment.Results Compared with the control group after the treatment, the total effective rate of the treatment group was higher(P<0.05).Compared with the control group, the serum postprandial 2h blood glucose, total cholesterol, triglyceride, low density lipoprotein cholesterol levels were lower(P<0.05), and high density lipoprotein were higher(P <0.05), the levels of whole blood viscosity high, middle and low shear, plasma viscosity, hematokrit and fibrinogen were lower(P<0.05), the serum levels of appeal index were lower(P<0.05), the blood flow of the dorsal artery, the diameter of the blood vessels and the ankle brachial index were higher (P<0.05), the peak velocity of the dorsal foot artery was lower(P<0.05) .There was no significant difference in the incidence of adverse reactions between the 2 groups.Conclusion Mudan granule combined with zinc sulfate tablet in the treatment of diabetic foot dorsal artery sclerosis was effective with high safety and it could improve ankle brachial index.

Objective To determine the characteristics and the best parameters of the retinal nerve fiber layer (RNFL) thickness measured by optical coherence tomography (OCT) in the early diagnosis of primary open angle glaucoma (POAG). Methods The thickness of RNFL was quantified by OCT (Carl Zeiss Meditec, Stratus OCT3000 version 3, RNFL3.4) in 71 POAGs(119 eyes) and 76 controls(148 eyes). Results The 9 OCT parameters with the largest AUCs and asymptatic significance less than 0.05 in ROC curve in the early diagnosis of primary open angle glaucoma (POAG) were thickness of 6, 7, 11 o’clock position, inferior, superior, average thickness, inferior maximum, superior average, inferior average. Conclusion In early stage of glaucoma, the thickness of RNFL measured by OCT provides a new index for the diagnosis of glaucoma. The best parameters seem to 6, 7, 11 o’clock position, inferior, superior, average thickess, inferior maximum, superior average, inferior average.

Computed tomography perfusion imaging can accurately and promptly provide hemodynamic parameters of brain tissues after stroke,and it has a good correlation with the accepted hemodynamic measurement,the parameters got from xenon-computed tomography, functional magnetic resonance imaging and positron emission tomography.Therefore,CTP has been widely used in predicting the studies of ischemic penumbra after stroke.

The prognosis and outcome in patients with intracerebral hemorrhage are closely correlated with perihernatoma tissue injury and recovery.The pathophysiological mechanisms after intracerebral hemorrhage are extremely complicated.There are regional blood flow changes in perihematoma tissues,but whether there is a similar“penumbra”as in cerebral ischemia remains uncertain.At present,studies in this field have mainly focused on the imaging detection of regional cerebral blood flow and metabolism.The study methods frequently used include single photon emission computed tomography,functional magnetic resonance imaging,magnetic resonance spectroscopy,positron emission tomography and CT perfusion imaging,etc.The application of these imaging techniques have provided some valuable methods for identifying whether perihematoma penumbra is present or not,but further studies are needed to confirm it.

Objective To study the expression of HIF-1α and survivin in gastric cancer,and to study their role in tumor angiogenesis,invasion and metastasis.Methods Immunohistochemieal technique was used to detect the expression of HIF-1α and survivin in 54 cases of gastric cancer.Results The expression rate of HIF-lα in gastric cancer (74.1%) was higher than that in normal gastric tissue (O),The expression of HIF-1α in gastric cancer was associated with TNM staging,invasive depth and lymph-node metastasis (P<0.05),The expression of survivin in gastric cancers was high (72.2%),and increased with invasive depth (P<0.01),clinical stages (P<0.01) and lymphatic metastasis (P<0.05).There was a positive correlation between expression of HIF-1α and Survivin (r:0.31,P<0.05).Conclusion HIF-1α and Survivin could be factors in the judgement of the degree of malignancy of gastric cancer and prediction of its prognosis.

Objective To study the molecular pathway of osteoblastic differentiation induced by substance P (SP), a neurotransmitter. Methods Mesenchymal stem cells were isolated and cultured, and treated with SP or its receptor (NK1) antagonist to induce osteoblastic cell differentiation, respectively. Alkaline phosphatase activity was determined; Osterix gene expression was detected by RT-PCR after 1-2 weeks for three times. The data of each culture condition were analyzed using SPSS12.0 statistical software to determine whether the differences between conditions were significant. Results After 4-5 days' culture, bone marrow stromal cells became spindle-shaped, triangular or polygonic. They covered the plate surface, formed extensive cell sheets in each group after 11-12 days of culture, and then induced differentiation to osteoblast. SP up-regulated the important transcription factor Osterix gene expression significantly (P<0.05). Conclusion The up-regulation of Osterix gene expression by SP may stimulate osteoblastic cell differentiation. SP's regulation depends on its receptor NK1.

Objective To investigate the effects of Xuebijing injection on the production of ICAM-1 and its counterreceptor(LFA-1)in ANP of rats at different time points.Methods ANP was induced by multiple points in- jection of 5% chenodeoxycholic acid into the pancreas,followed by ligation of pancreatic duct.Expressions of ICAM- 1 at different time points were assessed by immunohistochemistry method,the expressions of LAF-1 on neutrophils at different time points were detected by flow cytometer.The effect of Xuebijing injection was also evaluated by de- tecting the output of ascitic fluid,amylase level and histological changes in pancreas and lungs.Results The expres- sions of ICAM-1 in pancreas and lung of Xuebijing injection group decreased significantly than those in ANP group at most time points during the research period(P

Objective To explore the clinical aspects and related EEG characteristics of epilepsy (EP) in children with cerebral palsy (CP).Methods The clinical data and the EEG Results of seizure-interictal-period in 84 CP children complicated with EP were collected and compared with the data of 124 CP children without EP.Results Among 84 CP children complicated with EP, 46 cases (54.76%) were partial seizures. Followed 26 cases (30.95%) were infantile spasms, 6 cases (7.14%) were tonic-clonic seizures. Spastic CP children tended to be the most patients seizures in EP, it was 82.14%. Children with teraplegia CP were the most type among the spastic CP children compliated with EP. The abnormal rate of EEG of CP children complicated with EP was significantly higher than that of CP children without EP ( P＜0.001), and local epilptiform discharges and multilocal epileptiform discharges were main types.Conclusion Partial seizures is the most epilepsy type in CP children complicated with EP, and spastic CP is the main type. EEG abnormal ratio is obviously higher in the CP children complicated with EP than the CP children without EP. The types of abnormal EEG are mainly local epilptiform discharges and multilocal epileptiform discharges.

Objective To study the expression of Livin,a novel inhibitor of apoptosis protein(IAP)family member in children with acute lymphocytic leukemia(ALL).Methods Livin protein of 40 cases myeloid tissue of children with ALL and 20 cases that of non-leukemia children were assayed by streptomycin avidin-biotin-peroxidase complex staining immunohistochemical method in order to analyze the relationship between Livin protein expression and development of ALL.Results The positive rates of Livin protein expression was 40% in 40 cases ALL,but in control group,the positive rates of Livin protein expression was 5%.The difference of 2 groups was significant(P

Objective To study the expression of novel inhibitor of apoptosis protein(IAP) family member livin gene and its two isoforms(livin ? and livin ?) in brain tissue of children with gliomas.Methods Livin ? and Livin ? mRNA were detected by real time fluorescent quantitative reverse transcription polymerase chain reaction(RT-PCR) in brain tissue of 30 children with gliomas and 12 healthy children.Result The positive rate of Livin mRNA expression was 83.3%(25/30 cases)in 30 cases of children with gliomas,the positive rate of Livin mRNA was only 8.3%(1/12 case) in normal brain tissue,there was significant difference in 2 groups(P