1.Class Ⅱa Bacteriocins:Biosynthesis, Structure and Activity
Yan-Ni LV ; Ping-Lan LI ; Wei ZHOU ; Guo-Rong LIU ; Xing-Hua GUO ;
Microbiology 1992;0(05):-
Class Ⅱa bacteriocins can be considered as the major subgroup of bacteriocins from lactic acid bacteria, not only because of their large number, but also because of their activities and potential applications. They have first attracted particular attention as listericidal compounds and are now believed to be the next in line if more bacteriocins are to be approved in the future. The present review attempts to provide an insight into general knowledge available for class Ⅱa bacteriocins and discuss common features and recent findings concerning these substances.
2.Effect of advanced glycosylation end products on oxidative stress and MCP-1 in human renal mesangial cells.
Min FENG ; Cheng-Bo XU ; Jun-Ping WEN ; Gui-Fang LIN ; Qi LV ; Guo-Liang HUANG
Chinese Journal of Applied Physiology 2014;30(4):306-313
OBJECTIVETo investigate the effects of advanced glycosylation end products (AGEs) modified bovine serum albumin (AGE-BSA) on the expression of reactive oxygen species (ROS) and monocyte chemoattractant protein-1 (MCP-1) in human renal mesangial cells (HRMCs).
METHODSHRMCs were cultured in vitro with medium containing different doses of AGE-BSA or BSA (50,100, 200, 400 mg/L) for 48 hours, or with AGE-BSA (200 mg/L) for different times (12, 24, 48, 72 h). Immunocytochemistry assay was used to estimate the protein level of RAGE. The ROS in cells were measured by flow cytometry and the mRNA expression of MCP-1 were analyzed by semi-quantiative reverse transcription-polymerase chain reaction (RT-PCR) after treatment with AGE-BSA or BSA.
RESULTSThe protein level of RAGE was upregulated in the HRMCs with AGE-BSA. The expression of ROS and MCP-1 significantly enhanced by incubation of AGE-BSA in a time- and dose-dependent manner. The effects of AGE-BSA-induced up-regulation of ROS and MCP-1 level was significantly blocked by neutralizing antibodies to RAGE, while the expression of ROS and MCP-1 stood nearly unchanged after cultured with huamn IgG.
CONCLUSIONThe expression of ROS and MCP-1 in HRMCs is induced by AGE-BSA through RAGE, which may have potential effects in the pathgenic mechanism of diabetic nephropathy.
Cells, Cultured ; Chemokine CCL2 ; metabolism ; Glycation End Products, Advanced ; pharmacology ; Humans ; Mesangial Cells ; drug effects ; metabolism ; Oxidative Stress ; drug effects ; Reactive Oxygen Species ; metabolism ; Receptor for Advanced Glycation End Products ; Receptors, Immunologic ; metabolism ; Serum Albumin, Bovine ; pharmacology
3.The E-selectin,P-selectin and ICAM-1 expression at different time points after focal brain ischemic-reperfusion in rats
Guo-Hong ZHANG ; Chun-Lin WANG ; Ping LV ; Yong-Li WANG ;
Chinese Pharmacological Bulletin 2003;0(10):-
Aim To study the expression of P-selectin,E-selectin and ICAM-1 and the migration of leukocyte at different time points after focal brain ischemia-reperfusion,and explore the role of cell adhesion molecules in cerebral ischemia-reperfusion damage.Methods The model of focal cerebral ischemia-reperfusion was established with the occluding suture as described by Longa.Sham operation was performed without inserting the suture.Rats were decapitated under anesthesia at 4,8,12,24,and 48 h after ischemia-reperfusion.Brains were immediately removed and samples were handled for following application.Morphological changes of the brain tissue were observed through hematoxylin-eosin staining.The positive expressions of P-selectin,E-selectin,and ICAM-1 were observed and located using immunohistochemistry and immunofluorescent histochemistry technique,respectively.The expressions of P-selectin, E-selectin and ICAM-1 in infracted cortex were measured quantitively with Flow cytometry.The activities of myeloperoxidase in the infracted hemispheres represented the migration of leukocytes were detected through biochemical method.Results The positive cells of P-selectin,E-selectin,and ICAM-1 expressed at the same position of microvessels in the infracted hemisphere were significantly increased after ischemia-reperfusion.Compared with the sham operation group(P-selectin:(4.99?0.08) channel,E-selectin:(4.17?0.13) channel,ICAM-1:(4.17?0.13) channel),the expressions of P-selectin,E-selectin,and ICAM-1 at I/R 4 h((5.46?0.09) channel,(4.60?0.14) channel,(4.56?0.12) channel),I/R 8 h((5.87?0.24) channel,(5.08?0.14) channel,(5.41?0.22) channel),I/R 12 h((6.48?0.18) channel,(5.72?0.18) channel,(5.66?0.16) channel),I/R 24 h((7.16?0.11) channel,(6.09?0.09) channel,(5.61?0.09) channel), and I/R 48 h((5.82?0.28) channel,(5.37?0.25) channel,(5.27?0.16) channel) were increased(P
4.Effects of Different Extracts ofTangwang Mingmu Granules on High Glucose Induced VEGF and IL-1α Expressions in Vascular Endothelial Cells
Changying HAO ; Mingxia CHEN ; Wenbin MA ; Ping GUO ; Haibo LV ; Ye LIU ; Zenglin LIAN
Chinese Journal of Information on Traditional Chinese Medicine 2016;(1):56-59
Objective To observe the mechanism of action of different extracts ofTangwang Mingmu Granules on high glucose induced VEGF and IL-1α gene and protein expressions in vascular endothelial cells.Methods Human umbilical vein endothelial cells EA.hy926 were divided into six groups: blank, high glucose,Tangwang Mingmu Granules, extract 1 (glycoside and flavonoid), extract 2 (organic acid and polysaccharides) and extract 3 (alkaloids) groups. High concentration glucose was used to establish the high glucose model in EA.hy926 cells. The expressions of VEGF and IL-1α mRNA were detected by semi-quantitative RT-PCR. The contents of VEGF and IL-1α protein were tested by ELISA.Results The gene expression and protein levels of VEGF and IL-1α were significantly up-regulated under the high glucose condition (P<0.05). However, the above indicators were significantly reduced after the treatment ofTangwang Mingmu Granules. The activity of different parts ofTangwang Mingmu Granules was as follows: extract 1> extract 3> extract 2.Conclusion The action intensity of glycosides and flavonoids, alkaloids, organic acids and polysaccharides on VEGF and IL-1α expression inTangwang Mingmu Granules weakens in sequence.
5.AFLP analysis on genetic diversity of Haloxylon ammodendron in China.
Liang SHEN ; Rong XU ; Jun CHEN ; An-Ping CHEN ; Guo-Qiang ZHU ; Jia LV ; Wei WANG ; Tong-Ning LIU
China Journal of Chinese Materia Medica 2014;39(6):959-964
To determine the genetic diversity of Haloxylon ammodendron collected from 14 sites in 5 provinces, 103 H. ammodendron samples of 12 wild populations and 2 cultivated which collected from 14 sites in 5 provinces were analyzed by amplified fragment length polymorphism (AFLP) DNA markers. PopGen32 and NTSYSpc2.1 was applied to evaluate genetic diversity of H. ammodendron populations. The average percentage of polymorphic loci (PPL) of total H. ammodendron populations was 94.13%, the average Nei's gene diversity index (H(e)) from 14 populations was 0.308 0, and the Shannon's genetic diversity index (I) was 0.467 6. The results indicated that the genetic diversity of H. ammodendron populations was high. Genetic differentiation index (G(st)) was 0.313 8, and the gene flow (N(m)) was 1.093 5 at the population level. The level of gene flow of H. ammodendron showed it possessed the feature of wind-pollinated outcrossing plants. AMOVA analysis indicated that genetic variation of H. ammodendron was much higher within groups (89.34%) than that among groups (10.66%), moreover genetic variation within groups mainly occurred among populations in different producing areas (84.80%). Cluster analysis (UPGMA) was applied to generate dendrogram based on Nei's genetic distances of 14 populations. Samples from Xinjiang and Qinghai were clustered respectively as a clade for their distant genetic relationship, while Samples from Gansu, Inner Mongolia and Ningxia were clustered together for their close genetic relationship. Genetic diversity of H. ammodendron populations is high in China, and genetic differentiation among regions is small, thus abundance within this specie is high at this stage. Therefore, wild nursery and artificial cultivating in different areas are effective measures for the conservation and sustainable utilization of H. ammodendron resources.
Amaranthaceae
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genetics
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Amplified Fragment Length Polymorphism Analysis
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China
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Evolution, Molecular
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Genetic Variation
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Phylogeny
6.Item selection analysis based on quality of life scale in patients with viral myocarditis.
Xiao-chen GUO ; Jun-ping ZHANG ; Ya-ping ZHU ; Ya-nan ZHOU ; Shi-chao LV ; Nan XIAO
Acta Academiae Medicinae Sinicae 2012;34(2):116-125
OBJECTIVETo further select the items based on the pre-test version of quality of life scale in patients with viral myocarditis.
METHODSTotally 100 patients with viral myocarditis were enrolled in this study. Methodologies including frequency distribution, discrete trend, t-test, Cronbach's α coefficient, correlation coefficient and factor analysis were applied to select items from different perspectives.
RESULTSA total of 17 items were selected by frequency distribution method from the perspective of central tendency, 15 items were selected by discrete trend method from the perspective of sensitivity, 16 items were selected by t-test method from the perspective of sensitivity and discrimination, 16 items were selected by Cronbach's α coefficient method from the perspective of internal consistency, 12 items were selected by correlation coefficient method from the perspective of representation and independence, and 18 items were selected by factor analysis method from the perspective of representation.
CONCLUSIONItem selection of quality of life scale in patients with viral myocarditis was successfully conducted based on the clinical epidemiological data using a variety of statistical methods.
Adolescent ; Adult ; Female ; Humans ; Male ; Myocarditis ; virology ; Quality of Life ; Surveys and Questionnaires ; Young Adult
7.Effects of paeoniflorin on pathological changes in global brain ischemia model rats.
Rong SUN ; Yin-Ping YI ; Li-Li LV ; Zuo-Ping ZHANG ; Hu SUN ; Guo-Qing LIU
China Journal of Chinese Materia Medica 2007;32(23):2518-2522
OBJECTIVETo explore the effects of paeoniflorin on blood brain barrier and pathological changes in brain ischemia.
METHODMice were divided into sham operation group, model group, positive control group-Deng zhanhua tablet group and three different dose (high, middle, low-dose) groups of paeoniflorin. The neurological symptoms of rats were observed. The SOD of ischemic brain tissue, MDA BBB and EAA contents were determined. The ultrastructure on the brain cells was inspected by transmission electron microscope.
RESULTPaeoniflorin had the protetive effects on 4VO model of total cerebral ischemia. Paeoniflorin could obviously increase SOD content, reduce MDA content in rat brain-tissue and alleviate oxidative stress damage by cerebral ischemia on rat brain. Paeoniflorin could improve pathological changes of cell nuclear, perikaryon, mitochondria and myelin sheath, which was the morphologic basis of the protective effect on ischemia. Paeoniflorin could alleviate the incrense of EAA content caused by and hypoxia and inhibit the excitatory neural toxicity by EAA.
CONCLUSIONPaeoniflorin has the protection effect on the brain edema after cerebral ischemia, the oxidative stress damage on brain tissue, the ultrastructure lesions of cells and the BBB. The protective mechanism may be related to inhibiting intracellular calcium overload, anti-free radical and reducing EAA content.
Animals ; Behavior, Animal ; drug effects ; Benzoates ; isolation & purification ; pharmacology ; Blood-Brain Barrier ; drug effects ; pathology ; ultrastructure ; Brain ; blood supply ; drug effects ; metabolism ; Brain Ischemia ; pathology ; physiopathology ; prevention & control ; Bridged-Ring Compounds ; isolation & purification ; pharmacology ; Glucosides ; isolation & purification ; pharmacology ; Male ; Malondialdehyde ; metabolism ; Microscopy, Electron ; Monoterpenes ; Neuroprotective Agents ; isolation & purification ; pharmacology ; Paeonia ; chemistry ; Plants, Medicinal ; chemistry ; Random Allocation ; Rats ; Rats, Wistar ; Superoxide Dismutase ; metabolism
8.Mitochondria DNA 4977 bp common deletion in peripheral whole blood from healthy donors.
Ping WANG ; Yu Long LIU ; Lin HAN ; Feng Ling ZHAO ; Fei GUO ; Xi Ai WANG ; Yu Min LV
Biomedical and Environmental Sciences 2013;26(12):990-993
To investigate the distribution of mitochondria DNA 4 977 bp deletion, a common deletion (CD), in normal populations of Chinese, human peripheral blood samples from sixty healthy donors were collected, and levels of the CD in genomic DNA from the samples were detected using real-time PCR. The results showed that the CD was found in 27 health donors, with its positive rate being 45% (27/60). The CD ratio was between 0 and 0.00308%, and not affected by age and gender in sixty healthy donors. Our studies indicate that the CD ratio is low, and do not show the age-dependent accumulation and any gender difference in peripheral whole blood from the normal Chinese population.
Base Sequence
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DNA Primers
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DNA, Mitochondrial
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genetics
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Female
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Humans
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Male
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Real-Time Polymerase Chain Reaction
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Sequence Deletion
9.Study on medicinal plant allelopathy and soil sickness based on ecological niche.
Hao SUN ; Lu-ming HUANG ; Lu-qi HUANG ; Lan-ping GUO ; Jie ZHOU ; Dong-mei LV ; Yan ZENG
China Journal of Chinese Materia Medica 2008;33(17):2197-2200
Based on the conception and theory of ecological niche, authors analyzed the cause of the allelopathy and soil sickness of medicinal plants and the relationship between them. Methods to resolve problems in the cultivating medicinal plant was found, that is to construct the ecological niche based on allelopathy theory and avoid the soil sickness.
Agriculture
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Ecosystem
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Plants, Medicinal
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growth & development
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Soil
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analysis
10.Effects of storage time on magnolol and honokiol contents in bark of Magnolia officinalis.
Da LV ; Jin-ping SI ; Zai-kang TONG ; Bao-lin GUO ; Yan-feng JIANG ; Yu-qiu ZHU
China Journal of Chinese Materia Medica 2008;33(17):2087-2089
OBJECTIVETo reveal the relationship between the storage time of the bark of Magnolia officinalis and the content of phenols in it, and lay a theoretical foundation for the harvest, processing, management and storage.
METHODThe contents of magnolol and honokoiol in 15 bark samples, collected from the main producing areas in China, were determined in the time of freshly harvest and 3 and 10 years after respectively by HPLC method.
RESULTIt showed that within a certain period of time, bark storage was favorable to conversion and accumulation of phenols, that the content of magnolol tended to increase from year 0 to year 3, then followed by slight decrease with years on account of volatilization of phenols, but was still higher when the bark was stored for 10 years than that that when the bark was freshly harvested, and the content of honokoiol still tended to increase when the bark had been stored for 10 years.
CONCLUSIONThe phenols in bark of M. officinalis is quite stable and the bark can be stored for 10 years or longer.
Biphenyl Compounds ; analysis ; Drug Storage ; methods ; Drugs, Chinese Herbal ; chemistry ; Lignans ; analysis ; Magnolia ; chemistry ; Plant Bark ; chemistry ; Time Factors