1.Expression and role of ubiqutin carboxyl-terminal hydrolase L1 in lens with age-related cataract under oxidative stress
Chinese Journal of Experimental Ophthalmology 2012;30(6):534-537
Background Oxidative damage is a major cause of age-related cataracts,and the ubiquitinproteasome system is involved in lens differentiation and development.Ubiquitin carboxy-terminal hydrolase L1 (UCHL1),one of key enzymes of ubiquitin-proteasome system,was discovered to participate in the age related diseases and oxidative stress damage. Objective This study was to investigate the effects of UCHL1 on the formation and development of age-related cataract. Methods Lens capsule were collected from 24 patients with age-related cataract(including 12 cases of cortical cataract and 12 cases of nuclear cataract) during the surgery.Five normal lens capsule membranes were obtained from eye bank of Tongji University.Human lens epithelial cells (LECs) line (SRA01/04) was also collected in this study.Expression of UCHL1 in the lens epithelial layer of different samples was assayed using immunofluorescence technology.UCHL1 eukaryotic expressing vector was constructed and transfected into cultured SRA01/04 by liposome,and green fluorescent protein (GFP) eukaryotic expressing vector was transfected at the same method as the control group.UCHL1 over-expressing cells were then exposed to different concentrations (0.2,0.3,0.4 and 0.5 mol/L) of tert-butyl hydroperoxide (TBHP) for 24 hours and subsequently monitored for cell viability evaluation by MTT assay. Results Immunofluorescence showed that UCHL1 was expressed in human lens epithelial layer,but significantly different expressing levels were seen among normal lens capsular membrane,cortical cataract and nuclear cataract ( F =13.411,P =0.000),and UCHL1 expressing levels were lower in cortical cataract and nuclear cataract than the normal lens (P =0.000,P =0.000).No significant difference was found in UCHL1 expressing level between cortical cataract and nuclear cataract ( P =0.164).Western blot analysis verified that UCHL1 exhibited a stranger expression in the UCHL1 transfected group compared with the GFP transfected group,illuminating a successful transfection of UCHL1 in SRA01/04 cells.MMT assay revealed that the A570/630 value in UCHL1 transfected cells was significantly elevated in comparison with GFP transfected cells following the treatment of 0.3 mol/L TBHP. Conclusions UCHL1 has an antioxidative ability,and it might plays an important role in the progress of age-related cataract.
2.Protective effect of liposome carried prostaglandin E_1 on skin flap ischemia reperfusion injury in rats
Jie GUO ; Yongcheng XU ; Hongchi JIANG
Chinese Journal of Medical Aesthetics and Cosmetology 2001;0(05):-
Objective To explore the mechanism of protective effect of liposome carried prostaglandin E 1 (PGE 1) on rat skin flap ischemia / reperfusin(I/R). Methods Island flaps of abdomen of adult SD rats were established for ischmia reperfusion injury models. Weighting, immunocytochemistry and in situ hybridization were employed. Results It was found that treatment with liposome carried PGE 1 significantly alleviated I/R induced muscular edema, attenuated neutrophil accumulation in the I/R tissue ( P
3.Reversal Effects of Tumor Necrosis Factor ? and Interferon ? on MDR in K562 Cell Line Resistant to Adriamycin
Jie LI ; Weijian GUO ; Liangzhong XU
Chinese Journal of Cancer Biotherapy 1995;0(03):-
Objetive: To observe the reversal effects of tumor necrosis factor (TNF) ? and interferon (IFN) ? on multidrug resistance (MDR) in K562 cell line resistant to adriamycin (ADR) (K562/A02). Methods: After treatment with TNF-? and IFN-? respectively, K562/A02 sensitivity to ADR was investigated using tetrazolium dye assay. MDR1 gene expression was assayed by semiquantitative reverse transcription-polymerase chain reaction and immunocytochemistry staining. Intracellular ADR concentration was also observed with flow cytometry. Results: The reversal activity after treatment with TNF-? or IFN-? was found to be increased up to 6 and 5-fold respectively at 24 h, and the peak with the increase of 10 and 8-fold respectively was seen at the 48 h (both P
4.THE INFLUENCES OF GENISTEIN ON SYNAPTOSOME MEMBRANE FLUIDITY AND INTRASYNAPTOSOME CALCIUM IN OVARIECTATIZED RATS'BRAIN
Jianhua ZHU ; Kaihua GUO ; Jie XU
Acta Anatomica Sinica 1954;0(02):-
Objective To observe the changes of synaptosome membrane fluidity and intrasynaptosome free calcium in ovariectomized rats' brain and the therapeutical effects of genistein. Methods Thirty-six 3 month-old female Sprague-Dawley rats were randomly assigned to 4 groups:sham-operated,ovariectomized control,genistein and estradiol benzoate groups.Normal saline(50??l),normal saline(50??l),genistein(250??g)and estradiol benzoate (50??g)were subcutaneously injected respectively once every other day for 8 weeks,and then synaptosome membrane fluidity and intrasynaptosome free calcium in frontal and parietal lobe and hippocampus were detected. Results The intrasynaptosome free calcium of cerebral cortex and hippocampus synaptosomes of ovariectomized control group (?s)are (243.31?31.21)nmol/L and (305.10?54.31)nmol/L respectively.There are significantly statistical differences as compared ovariectomized control group with sham-operated,genistein and estradiol benzoate groups(P0.05).The synaptosome membrane viscosity(?)of hippocampal synaptosomes of ovariectomized control group(?s)is 3.03?0.39,which has significantly statistical differences compared with sham-operated and estradiol benzoate groups(P0.05).Conclusion The synaptosome membrane fluidity decreased while intrasynaptosome free calcium increased in ovariectomized rats' synaptosomes.Genistein can enhance the membrane fluidity and maintain intrasynaptosome free calcium in ovariectomized rats' synaptosomes.
6.Au@PVP Core-Shell Nanoparticles Used as Surface-Enhanced Raman Spectroscopic Substrate to Detect Malachite Green
Ningning XU ; Qin ZHANG ; Wei GUO ; Qintao LI ; Jie XU
Chinese Journal of Analytical Chemistry 2016;44(9):1378-1384
The core-shell nanopaticles of Au@polyvinyl-pyrrolidone ( PVP) with uniform size and controllabe shell-thickness were prepared by hydrothermal method. The core-shell nanoparticles could be assembled to be the monolayer array on Si substrate relying on the dispersion of core-shell nanoparticles arising from PVP shell. The malachite green ( MG ) absorbed by H-bond could be detected on the array under the electromagnetic enhancement of inner-core Au nanoparticles. Under the conditions of the optimum shell-thickness of Au@PVP and the appropriate absorbed time of MG, the detection of MG could be realized in the linear range from 1 × 10-10 mol/L to 1 × 10-5 mol/L with the correlation coefficient ( R2 ) of 0. 98. The detection limit was 10-12 mol/L. This method was applied to the determination of MG in tilapia fish fillets of Xiagang market. No MG was found in this real sample. The spiked recoveries of the sample ranged from 70. 8% to 126. 0%. This method is simple and accurate, and can be used for detection of MG in the fish.
7.Early diagnosis value of urinary NGAL in acute kidney injury in patients with severe traumatic brain injury
Peng HUANG ; Xu LIN ; Jie WANG ; Pengwei GUO ; Chunrong TANG
Chongqing Medicine 2014;(31):4203-4205
Objective To assess the predictive value of neutrophil gelatinase associated protein lipocalin (uNGAL) in urine for detection of acute kidney injury(AKI) in patients with severe traumatic brain injury. Methods Patients with severe traumatic brain injury from the ICU were collected from Jan. 2011 to May. 2013 in our hospital. 43 cases that met the RIFLE criteria for diagnosis of AKI in the ICU within 7 days were selected as AKI group. Another 43 cases that were matched for age ,gender ,illness severity , surgery method with AKI cases ,selected as non‐AKI group. The levels of uNGAL and Scr were measured when the patients admit‐ted in the ICU with 15 min ,at 24 h ,48 h ,72 h. the sensitivity and specificity of uNGAL and Scr for diagnosis for AKI were evalua‐ted by ROC curve. Results The incidence of severe traumatic brain injury AKI was 42. 16% (43/102). The uNGAL levels in the AKI group were higher when the patient stayed in the ICU longer and no obvious in the non AKI group. When admitted to the ICU 24 h ,the level of uNGAL(720. 32 ± 684. 25)ng/mL in AKI group was significantly higher than that (421. 92 ± 351. 20)ng/mL in non AKI group. The difference was statistically significant (P< 0. 05). The levels of Scr between two groups were not statistically significant. The area under ROC curve of uNGAL and Scr were 0. 879 (95% CI :0. 807 - 0. 949) and 0. 612 (95% CI :0. 493 -0. 731). When the cutoff value of uNGAL was 180 ng/mL ,the sensitivity and specificity were 0. 890 and 0. 823 respectively. The sensitivity was superior to Scr. Conclusion uNGALis superior to Scr for early diagnosis of AKI in patients with severe traumatic brain injury and it could be used as a biomarker for early diagnosis of AKI.
8.Investigation on Job Burnout in Doctors of Oral Health in Hainan Province
Xu ZHENG ; Wenqian GUO ; Yi CHEN ; Jie SU
Journal of Kunming Medical University 2016;37(8):131-134
Objective In order to find out the properly corresponding solutions of stomatology doctors' profession exhaustion,we investigated and analyzed the current situations and the influential factors.The solutions would help stomatology doctors improve the working efficiency and the enthusiasm to job.Methods The survey was conducted using MBI-GS questionnaire.Results Among all the participants 52% had a certain degree of emotional exhaustion,58% had emotional exhaustion,61% were cynic and had doubts about personal accomplishments.Females were even worse than the male in emotional exhaustion.Significant correlation was found between wage satisfaction and oral health care provider's emotions (P<0.05) No significant relationship was seen between the marital status and different nature of the unit,and the score was not statistically significant (P>0.05).No obvious difference wss observed in different professional titles.Concltsion In Hainan Area,the stomatology doctors have partly job burnout,according to these surveys,we now taking diversified and adjusted measures to make corrections and prevent the job burnout.
9.Preliminary Approach to the Service of Intravenous Drugs Allocation Centre
Wanhua YANG ; Weimin CAI ; Bing XU ; Jing GUO ; Jie FANG
China Pharmacy 2001;0(09):-
OBJECTIVE:To promote effective development of services in the intravenous drugs allocation centre and to bring the pharmacists'function into full play in the hospital pharmacy.METHODS:Information concerning services in the intravenous drugs allocation centre of our hospital and experiences from which was introduced,some problems and difficulties in the pharmacy services were analyzed.RESULTS&CONCLUSION:The centralized allocation and management of intra-venous drugs have ensured the safety and effectiveness of the clinical intravenous drug use and which have become the essential part of the pharmaceutical care with a core of rational drug use.
10.Observation effect of levocarnitine combined with shuxuetong in treatment of the patients with chronic renal failure
Lixin CHENG ; Yuzhi XU ; Yuqiang GUO ; Jie LV ; Junfang LI
Chinese Journal of Primary Medicine and Pharmacy 2010;17(21):2901-2902
Objective To study the effects and safety of levocarnitine and shuxuetong in treatment of the patients with chronic renal failure.Methods 94 patients with chronic renal failure were selected as research object,and they were randomly divided into group A(levocarnitine and shuxuetong group) 47 cases and group B(shuxuetong group) 47 cases.Group A were treated with levocarnitine oral solution and shuxuetong,and group B were treated with shuxuetong,then the percentage of effectiveness,incidence of adverse reactions of group A and group B and serum BUN,Scr,Hcy were analyzed.Results The percentage of effectiveness of group A was higher than that of group B,but the incidence of adverse reactions of the two groups had no difference,and the serum BUN,Scr,Hcy of group A and group B before the treatment had no differences(P>0.05),those of group A and group B after treatment were compared there were significant differences(all P<0.05).Conclusion The effects of levocarnitine oral solution and shuxuetong in treatment of patients with chronic renal failure were better.