OBJECTIVE:To study the isolation of lentinan,the average molecular weight of which ranged within400000to600000.METHODS:The fine lentinan was purified by2—diethyllaminoethyl—cellulose anion exchange column chro-matography,which was then isolated by dextran gel G-200column chromatography with the molecular weights determined by high performance gel chromatography.RESULTS:The average molecule weight of the isolated lentinan ranged from400000to600000.CONCLUSION:This method can be used for the isolation of lentinan.

OBJECTIVE:To probe into the relation between integrated pharmaceutical care and the drug use administration of inpatients with medical insurance.METHODS:The principle and the role of the integrated pharmaceutical care were in?troduced;and the problems in the drug use administration of inpatients with medical insurance were analyzed and some sug?gestions were put forward accordingly.RESULTS&CONCLUSION:The key in drug use administration of inpatients with medical insurance is to implement integrated pharmaceutical care in accordance with clinical pharmacy practice program and to keep the medical expenditures under control by means of principles and methods in pharmacoeconomics.

ObjectiveThis paper aims to probe into the effect of working and studying combined teaching reform of chnical medicine major.MethodsTwo classes containing 46 students respectively were selected at random as observation group and control group,from the six classes of Grade Two majoring in clinical medicine.The two groups of students were trained by adopting working and studying combined teaching model and traditional teaching model respectively,after which they were evaluated in terms of clinical theory,practical skills and operations,and exercitation achievement.ResultsThe observation group does better in practical skills and exercitation achievement than the control group,while they have no difference in clinical theory.ConclusionWorking and studying combined teaching model can improve students' clinical analytical ability,strengthen their awareness of medical morals,and help them achieve more in exercitation.

AIM: To investigate differentiation of CD34~+ cells in human umbilical blood into eosinophils under the condition of cell culture in vitro.METHODS: CD34~+ cells were separated and purified from human umbilical blood.The cells were divided into negative group,IL-5 group and allergic rhinitis serum group.The differentiation ability of the cells was measured by flow cytometry,HE staining and electron microscope at the first day,second day, 7th day,14th day and 28th day culture.RESULTS: The proportion of CD34~+ cells in IL-5 group and allergic rhinitis serum group were decreased at the second day.The proportion in allergic rhinitis serum group was lower than that in IL-5 group significantly.The typical structure of eosinophils was observed at the second day.CONCLUSION: The allergic patient serum and IL-5 induce differentiation of CD34~+ cells in human umbilical blood to eosinophils.

AIM:To analyze the recovery of visual cortex activation range of color vision in anisometropia amblyopia children after treatment by using blood oxygen level dependence-functional magnetic resonance imaging ( BOLD- fMRI ) and SPM8 software.
METHODS:Self-control study. This fMRI study directly compared activity in visual cortex produced by color monocular stimulation in 13 monocular anisometropia amblyopia children. The project of study was blocked design. The data of functions and anatomical MRI was stimulated by three-primary colors and was preprocessed and analyzed by SPM8 that based on MATLAB software. According to the data, we compared the change of the central area of color vision when first visit and 1, 2 ,4wk after treatment.
RESULTS: The BA17, BA18, BA19 and BA37 of amblyopia children were different activated after they accepted the three-primary colors stimulation (P<0. 01). After 4wk treatment, amblyopia children accepted red stimulation, the visual cortex activation range without expanding; after accepting green stimulus, on the left side of the visual cortex BA37, BA19, BA20, the average t value were 3. 5210, 3. 2716, 3. 1534 ( P< 0. 01 ); after accepting blue stimulation, the scope of the right side of visual cortex at BA19, BA18 were expanded to different extent, the average t value were 3. 7345, 3. 2701 (P<0. 01).
CONCLUSION: After 4wk treatment, visual cortex activation of color vision of anisometropia amblyopic children is expanded, but with low extent, after the short-term treatment of amblyopia, visual cortex activation of color vision has a certain degree of recovery but not obvious.

BACKGROUND:Cardiopulmonary exercise test plays a significant role in the evaluation of cardiopulmonary function, but it is limited by expensive equipments and professional personnel, and moreover, the subjects need to bear the maximal exercise intensity. As a result, it is extremely urgent to find a submaximal exercise test characterized by simple operation, low cost and easy to be popularized and used. OBJECTIVE:To compare the maximal oxygen uptake in cardiopulmonary exercise test and 6-minute stair climbing and descending test. METHODS:Sixty-seven volunteers were recruited to undergo the cardiopulmonary exercise test using the Bruce protocol, and then, the maximal oxygen uptake was detected. After that, al the participants were subjected to 6-minute stair climbing and descending test, folowed by determination of the maximal oxygen uptake. RESULTS AND CONCLUSION:The maximal oxygen uptake in the cardiopulmonary exercise test was significantly higher than that in the 6-minute stair climbing and descending test (P < 0.01), and there was a highly positive correlation and consistency between the maximal oxygen uptakes in the two tests (r=0.911,P < 0.01). Therefore, 6-minute stair climbing and descending test can be used to detect the maximal oxygen uptake, which may become an effective method for evaluating cardiopulmonary function.

Objective:To summarize and analyze the clinical status of tumor necrosis factor-α (TNF-α) antagonists.Methods: The clinical trials of TNF-α antagonists registered before August 2,2016 were searched on the National Institutes of Health (NIH) Clinical Trials Register,and SPSS17.0 software was used for statistical analysis of the data.Results: A total of 306 TNF clinical trials were conducted in the world,19 of them were in China.The main research diseases for bone and joint disease,autoimmune diseases,infections,blood tumors.There were 241 items in intervention study,and 56,7,55,60 items in 178 cases,accounting for 73.9% in Ⅱ,Ⅱ/Ⅲ,Ⅲ,Ⅳ,respectively.The interventional study of TNF-α antagonist clinical trials mainly focused on intervention research,The main purpose of the practical application of the process after the listing of further validation and evaluation of drug efficacy and safety.Conclusion: There are many kinds of TNF-α antagonists,and clinical indications are wide.The design of clinical trials is diversified and mature.

Objective:To evaluate the effect of preoxidation on the bonding strength of titanium to porcelain.Method:12 titanium plates(25 mm?3 mm?0.5 mm) were pre-oxidated according to usual preoxidation procedure and 12 without preoxidation were used as the controls.The samples(6 in each group) were then bonded with bonding porcelain,opaque porcelain,dentin porcelain and glaze of Duceratin(Degussa) or Vita titankeramik(Vita) respectively.The bonding strength of Ti/porcelain was evaluated using three-point bending test according to ISO 9693 1990 standard. The interface of Ti/porcelain bonding was observed by SEM.Results:Bonding strength(MPa) of Ti/Duceratin preoxided group and the corresponding control was 41.910?2.778 and 33.097?5.297(P0.05),respectively.SEM observation showed inter-lock and tight bonding of Ti/porcelain in pre-oxidated interface,more cracks and gaps between Ti and porcelain in non-pre-oxidated interface.Conclusion:Pre-oxidation may improve bonding strength of Ti/Duceratin system, while has no significant effect on bonding strength of Ti/Vita titankeramik system.

Objective To establish a quantitative biomechanical relationship between the anterior cruciate ligament deficiency and the posterior horn of medial meniscus. Methods 10 human cadaveric knees were examined using an in vitro knee-testing apparatus. In response to a combined 134 N anterior and 200 N axial compressive tibial load, the in situ forces in the posterior horn of medial meniscus were measured. Testing was performed on 10 knees at multiple angles of flexion (30?, 60?, 90?) before and after resection of anterior cruciate ligament. Results The resultant forces on the posterior horn of medial meniscus were as follows: intact ACL knees, (22.8?11.5) N in 30? flexion, (27.1?16.3) N in 60? flexion, (26.7?14.5) N in 90? flexion. ACL deficiency knees, (87.3?43.9) N in 30? flexion, (77.7?43.3) N in 60? flexion, (66.2?40.1) N in 90? flexion. The resultant forces significantly increased as a result of ACL deficiency(P