Objective To explore the expression of drug transporter organic anion transporting polypeptide2(Oatp2) and P-glycoprotein(P-gp) in brains of chronic epileptic rats induced by lithium-pilocarpine.Methods Chronic epilepsy modles were elicited after status epilepticus(SE) induced by lithium-pilocarpine in adult Wistar rats.The expressions of Oatp2 and P-gp were detected by immuno-histochemistry(SABC) method.Results Positive expression of Oatp2 was predominantly in the plexus epithelial cells and(blood-brain) barrier endothelial cells.Weak staining for Oatp2 was also detected in the neurocyte.In contrast,apparent expression of(P-gp) was seen in brain capillary and neurocyte only.Compared with normal control rats,expression of Oatp2 on the plexus epithelial cells and brain capillary in chronic epileptic rats was significantly decreased(P

Objective To investigate the effects of electro-acupuncture (EA) on remyelination after sciatic nerve was transected in rats, so as to explore the possible mechanism of EA in treatment of peripheral nerve injury. Methods After transection of rat's sciatic nerve, the nerve regeneration chamber was built,then the rats were given EA stimulation at frequencies of 5 Hz and 100 Hz, respectively, and Solochrome Cyanine staining, HE staining of nerve fibers, immunohistochemical staining and semi-quantitative image analysis technique were employed to observe the remyelination of the nerve fibers and S-100 protein expression of Schwann cells. Results EA could significantly promote remyelination of the nerve fibers, elevate S-100 protein expression of Schwann Cells in repairing process of transected sciatic nerve of rats. The therapeutic effect of 5 Hz EA group was better than that of 100 Hz EA group. Conclusion EA is an effective way for the treatment of peripheral nerve injury. It can promote proliferation of Schwann cells and nerve fiber myelination. The therapeutic effects of EA are frequency-depondent.

Objective To evaluate the rate and types of human papillomavirus (HPV) in vulvar intraepithelial neoplasia (VIN).Methods We detected HPV DNA in 67 lesion tissues collected from 40 VIN patients with PCR based reverse line blot hybridization and DNA sequencing. The PCR using GP5/GP6 primers in HPV L1 region resulted in a 150-bp fragment. When the PCR did not amplify the target DNA,another PCR using SPF1/SPF2 primers was performed to amplify the 65-bp fragment.Results HPV DNA amplified with primers GP5/GP6 was positive in 52.2% (35/67) of the lesions. Of the 32 negative lesions,26 (81.2%) were positive for HPV DNA amplified with SPF1/SPF2 primers. The total positive rate was 91.0% (61/67). Ninety percent of the HPV belonged to high risk types. Sequencing data showed that the genotypes of 31 mono-infection lesions were the same as those of the reverse line blot results,yet the sequences in 4 multi-infection samples could not be determined. Of the 26 SPF PCR products,24 could be sequenced. 80.6% (25/31) patients with multiple lesions displayed the same genotype,suggesting HPV in the different lesions from the same patient was monoclonal.Conclusion The high risk type of HPV is associated with vulvar intraepithelial neoplasia and may play an important role in the development of invasive vulvar carcinoma.

Objective To explore the relationship between the tiny enhanced foci in hematoma and hematoma expansion at the acute stage of intracerebral hemorrhage (ICH).Methods CT routine and enhanced scan were applied in 36 ICH patients which onset ≤5 h,and CT examined again follow-up 1 or 2 d.The neurological function was evaluated by European Stroke Scale(ESS)at the 1st d and 21st d after onset.Results The tiny enhanced foci in hematoma were found in 11 csaes by the first CT scane.Follow up CT scane,the hematoma expansion was occurred in 10 cases,including 8 cases with tiny enhancing foci in hematoma.The incidence of hematoma expansion in ICH patients with tiny enhancing foci in hematoma(8 cases,72.7%) was significantly higher than in ICH patients without tiny enhancing foci in hematoma(2 cases,8.0%)(P

0.05). In hFOB, the expression of P53 was not detected, and that of P63 was weakly positive. The expression of P53 and P63 in HOS transformed by niekel sulfate were higher than that in normal HOS (P

Objective To explore the pathogenesis-related imprinted genes in osteosarcoma and investigate the role of imprinted genes expression in osteosarcoma pathogenesis.Methods The model of malignant transformed immortalized human osteoblastic cells was established and the malignant transformed cells were collected on day 40,55,70,90.The untreated cells passaged from the normal cells on day 90 served as control.After the total RNA extraction,the finally synthesized biotinylated cDNAs were hybridized to CapitalBio Genechip CAP-F0017.2 of the differentially expressed genes.Two genes were chosen at random to further confirm the array results using the SYBR Green real-time PCR in samples of control,day 40 and day 90. Results By an entrance limit of ≥2.0 or ≤0.5,ten imprinted genes which biological functions are mainly related with cell growth/maintenance and signaling transduction were detectable for notablely differential expression,including down-regulated genes of CD81,GRB10,NDN,MEST and up-regulated genes of H19,MKRN3,SLC22A1L,TSSC3,CDKN1C in the malignant transformation of immortalized human osteoblastic cells.The array results were further confirmed by the real-time RT-PCR.Conclusion Ten imprinted genes were detectable for notablely differential expression.Our results will promote our understanding of the molecular mechanisms of imprinted genes in the course of osteosarcoma pathogenesis.

Objective To know the air supply quality of central air-conditioning ventilation system in building before and after cleaning.Methods Three sets of central air-conditioning ventilation systems were selected from a building randomly and 3 air supply ways were selected in each system for the determination of inhalable particulate matter(PM10) and microorganisms in air before and after cleaning.Results After cleaning,the unqualified rate of air quality increased from 11.1%(before cleaning) to 77.8%.The mean value of PM10 increased from 0.051 mg/m3(before cleaning) to 0.083 mg/m3,the median of total account of bacteria and fungus increased from 150 cfu/m3 and 13 cfu/m2(before cleaning) to 570 cfu/m3 and 110 cfu/m3 respectively.Conclusion The air supply quality of central air-conditioning ventilation system in building may be damaged by cleaning if the operation is not in the right way.

Objective To understand the distribution character of bacteria in the air of cosmetic workshops and present scientific data for the GMP establishment of cosmetic factories.Methods Of cosmetic factories 11 equipped with air depuration systems (ADS)(type I),13 with no ADS(type II)and 9 producing powder products (such as kermes,face mask,etc.) with no ADS(tyoe III)were chosen,distribution character of bacteria in the air was studied there.The bacteria samplings were conducted before and after operation and in winter and spring,summer and autumn respectively.Results The bacterial count in the three kinds of factories was:type I

Objective To explore an assessment questionnaire for assessing caregiving knowledge of primary caregivers in children with nephrotic syndrome. Methods Delphi method was used to review the literature. Ten primary caregivers of children with nephrotic syndrome were interviewed and five medical, nursing experts were pre-surveyed to form an expert consultation questionnaire. Sixteen experts from some provinces were selected to carry out two rounds expert consultation. To screen the dimensions, factors, entries of the questionnaire and to analyze the reliability and validity of the questionnaire. Results The positive coefficient was 100%, the expert authority coefficient was 0.95 and the expert co-ordination coefficient W test was P<0.05 of the two rounds expert consultation. The questionnaire data included 6 dimensions, 26 factors, 106 items. The basic knowledge of disease, diet knowledge, medication knowledge, personal hygiene and infection prevention, activity and rest, home care constitute the dimensions. The Cronbach α coefficient was 0.872, the test-retest reliability was 0.823, the split-half reliability was 0.855, and the average overall validity index of the questionnaire was 0.948. Six common factors were extracted for exploratory factor analysis and the cumulative variance contribution> 65.633%. Conclusions The questionnaire of caregiving knowledge of primary caregivers of children with nephrotic syndrome developed by Delphi method has high reliability. The questionnaire is of practical significance for evaluating the care of primary caregivers of children with nephrotic syndrome.

The paper first analyzes the current status of and existing problems with the management personnel in domestic third-tier hospitals and expounds from such angles as work motives, knowledge structure and personality the necessary qualities that hospital management personnel should possess. Then it argues on the basis of analyses from such perspectives as methodology, disciplinary system development and macro policy environment that only through appropriate training in hospital management can the current status of the management personnel in domestic hospitals be changed, the professional advancement of the management personnel in the third-tier hospitals be genuinely realized, and the support and impetus for maintaining the competitiveness of domestic third-tier hospitals in the medical market be provided.