ObjectiveTo explore the clinical effect and safety evaluation of marvelon on dysfunctional uterine bleeding in adolescent patients.Methods120 adolescent patients with dysfunctional uterine bleeding were randomly divided into control group and experience group with 60 patients in each group.Control group was given oral administration of premarin and experience group received marvelon oral treatment.Clinical effect,the time of control bleeding and completely stop bleeding and incidence of adverse reaction were compared between two groups.ResultsThe total effective rate in experience group was much higher than that in control group (100.0％ vs 90.0％,x2 =6.316,P ＜ 0.01 ) ;The time of control bleeding and completely stop bleeding in experience group were much lower than that in control group( t =6.638,8.542,all P ＜ 0.01 );The incidence of adverse reaction in experience group and control group were 11.7％ and 65.0％ respectively with significant difference( x2 =36.099,all P ＜0.01 ).ConclusionMarvelon on dysfunctional uterine bleeding in adolescent patients is beneficial to rapid and effective hemostasis with less adverse reaction rate and better tolerance.

Objective To investigate the cost-effectiveness of four drugs in treatment of bacterial vaginitis and improve clinic rational drug use.Methods 361 bacterial vaginitis out-patients were treated with lactobacillus vaginal effervescent tablets(92 cases),lacidophilin vaginal capsules(88 cases),shuangzuotai vaginal effervescent tablets(90 cases),metronidazole vaginal effervescent tablets(91 cases).The therapeutic effect,drug cost and therapeutic effect were evaluated by cost effectiveness analysis 7d after treatment.Results The overall response rate of lactobacillus vaginal effervescent tablets,lacidophilin vaginal capsules,shuangzuotai vaginal effervescent tablets,metronidazole vaginal effervescent tablets were 78.26％,76.14％,83.33％ and 78.02％,respectively.The overall response rate was no significant difference between four groups (0.06),and metronidazole vaginal effervescent tablets had the lowest cost effectiveness rate (C/E,0.26).Conclusion The metronidazole vaginal effervescent tablets is better schedule in treatment of bacterial vaginitis.

[Summary] From November 2013 to March 2014, 3D laparoscopic operations were carried out in 14 cases in the department of urology of our hospital .The operation went smoothly , without conversions to laparotomy .The operation time was 54-466 min, with an average of 180 min.The intraoperative blood loss was 5 -400 ml, with an average of 70 ml.No pressure sores or operation complications occurred.The medical staff participating in the operation generally felt a good 3D vision, without sense of vertigo.We deem that during urological operations which have different operation postures , it is very important to protect both laparoscopic system and operation area from pollution .Timely and properly wearing 3D glasses, application of one-eye-detection method for lens sharpness , and the switching between 2D and 3D modes can reduce and relieve visual fatigue .

Objective:To establish an HPLC method for the simultaneous determination of methionine, vitamin B1 and vitamin B2 in Lingzhi Erwei methionine capsules. Methods:The determination was performed on a Ultimate? AQ-C18 column(250 mm × 4. 6 mm, 5 μm,Welch Inc. ) with the detection wavelength at 220 nm. The mobile phase was composed of methanol and 0. 07 mol·L-1 sodium heptanesulfonate solution (14 ml triethylamine diluted with water to1 000ml, adjusting pH to 3. 5 with diluted orthophosphoric acid) with gradient elution, and the flow rate was 1. 0 ml·min-1. Results: Methionine, vitamin B1 and vitamin B2 was in good linearity within the range of 0.527 0-1.510 0 mg·ml-1 - (r =0.999 9), 0.045 52-0.136 56 mg·ml-1 (r =0.999 9) and 0.010 10-0. 060 58 mg·ml-1(r=0. 999 9), respectively. The mean recovery was 100. 5%, 97. 7% and 101. 5% with RSD of 0. 5%, 1. 0%and 1. 4%(n=9) accordingly. Conclusion:The method is simple, accurate, reliable and appropriate in the simultaneous determina-tion of methionine,vitamin B1 and vitamin B2 in Lingzhi Erwei methionine capsules.

The diagnostic criteria of diabetes has been changed several times in the latest thirty years.The diagnostic criteria is based on plenty of epidemic studies,especially the relationship between the glucose level and the microvascular complications.However,there are many problems in the clinical practice of using the recent diagnostic criteria(1999 WHO criteria).Glycosylated hemoglobin(HbA1c) has been recommended as a diagnostic criteria in the 69th ADA.

Objective To study the effect and the mechanism of baicalin on invasion and metastasis of(human) hepatocellular line BEL-7402.Methods Human hepatocellular line BEL-7402 were cultured and treated respectively with 5,10,20 mg/L baicalin for 48 h.The invasion and adhesion of the BEL-7402 cells were examined in Boyden chamber,cell migration and morphology were also observed.The expression of MMP_(2),TIMP_(2),_()integrin ?_(1,)and E-cd proteins of human hepatocellular line BEL-7402 was determined by flow cytometry.Results Baicalin inhibited invasion,adhesion and migration of hepatocellular line BEL-7402 in vitro in a dose dependent manner(P

Objective: To investigate the effect of GLP1 on the blood glucose in type 2 diabetic rats,and its protective effects on the islet B cells.Methods: Thirty rats were divided into three groups: spontaneous type 2 diabetes animal model OLETF rats,GLP-1 [from the twelfth week 56 ?g/(kg?d),sc] therapy group and LETO rats as control.In the 14th and 20th weeks,standard OGTT including fast and 2 h-plasma glucose were measured respectively.In the 14th weeks,3 rats from each group were killed randomly,and the rest of the rats were killed until the 20th week.Immunostaining with the marker of PCNA,TUNEL,and insulin assessed metabolic changes in the islet. Ultrastructure of the B cell was observed with the electronic microscope.Results: In the 14th and 20th week,AUC for insulin were higher in treated animals(10.86?1.56 vs.9.07?1.28,P

AIM: To establish the technique of precision-cut fibrotic liver slice (PCLS) and grope the optimal cultural conditions for researching the liver xenobiotic metabolism in vitro and the drug interaction. METHODS: Complex factors (higher fat diet, alcohol and CCl 4) were used to make the animal model of liver fibrosis. Fibrotic liver slices were prepared and cultivation system was established. Lactate dehydrogenase (LDH) leakage, glutathione S-transferase (GST) activity and 3[4,5-Dimethythiazole-2-yl]-2,5- diphenyltetrazolium bromide (MTT) reduction were chosen as indexes to assess the viability of the slice in different thickness, medium pH and cultural time. RESULTS: Rats were in earlier hepatic fibrosis after administration for 3 weeks. When the thickness of slices was 300 ?m and medium pH was 7.0, the LDH leakage, GST activity and MTT reduction could maintain on a steady level in 6 h. CONCLUSION: A 300 ?m of thickness, 7.0 of medium pH and 6 h of cultural time are the optimal slicing and culturing conditions for fibrotic liver slice.

As more attention is given to people's life and health,China continues to increase investment in scientific research to improve the level of medical research. However,the efficiency of the investment is unknown.In this study,we used the method of data envelopment analysis (DEA) to investigate the input-output relative efficiency of the projects funded by National Natural Science Foundation of China and completed in 2009 at 17 research institutes.We found 11 institutes to be DEA efficient,and 6 to be inefficient.It also showed that adjustment of research structure and setting performance objectives may improve the level of research management and increase the efficency of the investment.

Objective To probe into the methods of postoperative nursing care for the patient undergoing domino liver transplantation.Methods The key of postoperative care included:monitoring of vital signs,the condition of cruor and the state of drainage rigorously; keeping the balance of intake and output; taking the immunosuppressive drugs correctly; proper diet; prevention of complication;giving psychological nursing.Results The patients passed the perioperative period successfully and were all cured.Conclusions To aim at the characteristic of this operation,implement holistic nursing care,is an important link to assure the patients of domino liver transplantation to recover smoothly after the operation.