Objective To investigate the change ofpermeability surfaces (PS) of CTperfusion(CT-p) before chemotherapy and early period treatment with chemotherapy (the first cycle of chemotherapy) in advanced non-small cell lung cancer and explore the correlation with the efficacy of chemotherapy.Methods Eighty patients with advanced non-small cell lung cancer which confirmed by pathology were taken standardized treatment by first-line drug.All patients performed with the CT-p technique before chemotherapy and one cycle after chemotherapy.The efficacy was evaluated by RECIST after 4 cycles of chemotherapy,the patients were divided into complete remission (CR) + partial response (PR),stable (SD),development (PD) according to the results and the change of PS was compared among these groups before chemotherapy and after 1 cycle of chemotherapy.Results Seventy-four patients' values of PS were useful.The remission rate and the stability rate was 32.4％ (24/74) and 78.4％ (58/74) respectively after 4 cycles of chemotherapy.PS after 1 cycle of chemotherapy in CR +PR (24 cases) and SD (34 cases) was lower than that before chemotherapy (12.38 ± 1.86 vs.18.13 ± 2.45,13.24 ± 1.97 vs.17.93 ± 2.38),and there was significant difference (P ＜0.05).There was no obvious change in PS before chemotherapy and after 1 cycle of chemotherapy in PD (16 cases),and there was no significant difference(P＞ 0.05).Conclusions The value of PS which reflects the change of perfusion in tumor vessel at early chemotherapy and the prognosis of the non-small cell lung cancer.Use the change of PS to predict the prognosis of patients with chemotherapy.It could be the norm of calculating the curative effect of chemotherapy in non-small cell lung cancer.

Hepatocellular carcinoma (HCC) complicated by arterioportal shunt (APS) is commonly seen in clinical practice,with an incidence rate of 28.8％-63.2％.It is manifested as abdominal pain,diarrhea,and ascites and can also lead to serious complications of portal hypertension including gastrointestinal bleeding.Although there are various therapeutic methods,they tend to have poor clinical effects.APS is one of the most important causes of death in patients with HCC.This article introduces the etiology,typing,clinical manifestation,and therapies of HCC complicated by APS and points out that although there are various therapeutic methods for HCC complicated by APS,interventional treatment remains the most important method.The exploration of interventional treatment helps to improve patients' prognosis.

Objective To observe the clinical efficacy of application at Shenque (CV8) in treating primary dysmenorrhea due to blood stagnation.Method Sixty patients with primary dysmenorrhea due to blood stagnation were enrolled and randomized into a treatment group and a control group to receive the corresponding intervention, started 3 d prior to menstruation. The treatment group was intervened by application at Shenque with Chinese herbal medicinewhile the control group was by orally taking Ibuprofen sustained release capsules, successively for 3 menstrual cycles. The pre-treatment, short-term and follow-up dysmenorrhea scores and traditional Chinese medicine symptom and sign scores for blood stagnation were recorded, and the clinical efficacies of the two groups were observed.Result The short-term total effective rate was 93.3% in the treatment group, slightly lower than 96.7% in the control group, while the long-term therapeutic efficacy in the treatment group (93.3%) was obviously higher than that in the control group (50.0%). The changes in the symptom and sign scores for blood stagnation in the treatment group were more significantthan those in the control group, especially in the scores of menstruation amount, blocked menstrual flow, color of menstrual blood and blood clots (P<0.01). No adverse events occurred during the whole study. Conclusion Application at Shenque can effectively release menstrual pain and improve the symptoms of dysmenorrhea due to blood stagnation.

Objective To explore the influences on biological function induced by hyperthermia in human lung cancer cell line H1299, and to investigate the possible molecular mechanism. Methods H1299 cells were cultured in vitro and divided into 2 groups. The cells in culture flasks of hyperthermia group were immersed into a water bath at 43 ℃for 1 h, and the cells of control group were cultured at 37 ℃. The cell growth was detected by CCK8 assay, and the cell cycle and apoptosis rates were detected by flow cytometry [propidium iodide (PI) staining and PI/Annexin V staining]. The effects of hyperthermia on migration and invasion abilities of H1299 cells were determined by Transwell migration and invasion assays, respectively. The expression of LLGL1 was measured by Western blot. Results The cell cycle had no significant difference between the two groups, but the apoptosis rate was significantly higher in hyperthermia group [(24.81 ±2.80) %] than that in control group [(11.73 ±1.55) %] (t= 7.709, P= 0.0021). The migrating cell number was decreased in hyperthermia group (25.67±4.81) than that in control group (85.00±10.31) (t=5.182, P=0.0066). The invasive cell number was also decreased in hyperthermia group (22.00±2.08) than that in control group (108.3.0±10.14) (t=8.342, P=0.0011). The expression level of LLGL1 protein in hyperthermia group was 4.2 times that in control group(t=3.028, P=0.0389). Conclusion Hyperthermia induces the cell apoptosis and inhibits migration and invasion abilities of H1299 cells, which maybe associate with increasing LLG1 expression.

Gastric cancer is a kind of disease with high incidence and mortality rate,most gastric cancer cases are diagnosed in an advanced,and chemotherapy is the main treatment.Drug resistance is one of the most important causes of therapy failure in gastric cancer patients.It has revealed that the mechanism of tumor resistance mainly involves alterations in cell cycle and proliferation,enhanced DNA repair capacity,defective apoptosis,damaged host immune,altered metabolism of drugs and so on.In recent years,an important molecule-microRNA(miRNA)that regulates the biological behavior of cell has attracted more and more attention.Studies have confirmed that numerous miRNAs can regulate the drug resistance of gastric cancer cells via BCL-2 signaling pathway,PTEN/PI3K/AKT pathway,ABCB1(MDR1/P-gp),autophagy,cell cycle and so on.

OBJECTIVE To explore the predisposing factors of pharyngo-cutaneous fistulas after surgical treatment of laryngeal cancer. METHODS A retrospective study of 757 patients who underwent laryngectomy during a 10-year period was carried out. RESULTS The occurrence rate of post-laryngectomy pharyngo-cutaneous fistula was 4.2 %. Single-factor analysis showed that parameters related to the occurrence rate of post-laryngectomy pharyngo-cutaneous fistula included clinical stage of tumor, appearance of post-operative infection, undergoing of pre-operative radiotherapy, and pre-operative nutrition states. CONCLUSION Pharyngo-cutaneous fistulas appears more frequently in patients with T3 and T4 tumor and who underwent pre-operative radiotherapy. Skillful operative technique and effective prevention of post-operative infection may contribute toward reducing the incidence of pharyngo-cutaneous fistulas after surgical treatment of laryngeal cancer.

Objective To analyze the onset, clinical manifestation, causation, complications of pediatric uveitis. MethodsOne hundred and two patients with uveitis under 16 year-old were retrospectively studied. They visited the clinic in Peking University First Hospital from November 1979 to December 2008. Their age ranged from 2.5 to 16 years old, with a mcan of 11.9 years. Routine exam was carried out, including visual acuity, slit lamp, fundus, and laboratory workup. The diagnosis and classification were made by the anatomic location according to the standard of The International Uveitis Working Group. The data of disease history, age of onset, manifestation, recurrence, causation, systemic diseases, complications, and lab examination were analyzed. Results A total of 102 patients (170 eyes)with pediatric uveitis were included in this study, 68 patients (66.6%) were bilateral cases. Anterior uveitis represented in 38 patients (37.3% ), intermediate uveitis in 19 (18.6%), posterior uveitis in 10 (9.8%),and panuveitis in 35 (34.3%). The disease duration was from five days to 2.4 years, with a mean of 3.6 months. The follow-up time was two weeks to more than ten years. The first three causes of pediatric uveitis were juvenile idiopathic arthritis, Vogt-Koyanagi Harada disease, and Behcet's disease. 36 patients were found with complications, and among them 19 had complicated cataract, seven had secondary glaucoma, five had corneal band dystrophy, 12 had iris synechia (both anterior and posterior), one had retinal detachment, two had eye atrophy, and one patient with juvenile idiopathic arthritis had bilateral femoral head necrosis because of the use of steroid and hip joint was replaced. There were ten children suffering more than two complications. Conclusions Pediatric uveitis is a possible blindness disease with variety of etiology and manifestations, and tends to cause complications. Early and special attention must be taken to avoid serious consequences.

Different from the general local hospital,the affiliated hospital of medical school plays double social roles that it not only serves as a teaching hospital but also a medical uniL Every medical staff in it shoulders the responsibility of teaching,researching and medical treatmenL According to the relative independence of affiliated hospital in comprehensive university,the particularity of professional and technical post,the multiplicity of post task,as well as problems existed in the current appraisal and appointment of professional and technical posts such as lacking objectivity and maneuverability in clinical skills examination and evaluation.the paper tries to put forward a new mode of clinical skills examination and evaluation system in accordance with the characteristics of affiliated hospital.

Various kinds of flavanoids such as flavone,flavonol,flavanone,isoflavone,flavanol and so on,have protective effects on the liver injuries,which are induced by chemicals,drugs,immunostimulation,alcohol and hepatic ischemia reperfusion in some degree.These kinds of protection are relevant to the function of flavanoids such as elimination of free radical,resistance of oxidation,resistance of lipid peroxidation,adjustment of immune function and so on.The study of the effect of flavanoids on animal experimental liver injury plays an important role in the development of drugs for prevention and treatment of liver diseases.This article is to summarize the research progress of the effect of flavanoids on experimental liver injury of animals.

Objective To investigate the influence of insulin pump on type 2 diabetes mellitus.Methods 51 patients were randomized into two groups: 31 cases controlled with insulin pump,20 treated with oral antidiabetic drugs.Serum level of C-peptide concentration was observed.Results There was a markedly increase in C-peptide level in both insulin treating group(P<0.001) and oral drug group(P<0.01).Half an year later,the insulin group was improved in β-cell function better than oral drug group.Conclusions Several days controlling of severe hyperglycemia with insulin pump could not eliminate the β-cell inhibition of glucose toxicity.Newly diagnosed severe type 2 diabetes(FPG≥14 mmol/L) could be treated with insulin for several months.