Objective:To explore improving effect of Danqi soft capsule on hemorheology in patients with coronary heart disease (CHD) and its myocardial protection effect .Methods:A total of 84 CHD patients treated in our hos‐pital from Dec 2013 to Dec 2014 were enrolled .They were randomly divided into routine treatment group (n=42) and combined treatment group (n=42 ,received Danqi soft capsule therapy based on routine treatment group ) .He‐morheological indexes ,ECG and inflammatory factor levels [C reactive protein (CRP) ,tumor necrosis factor α(TNF‐α)] were measured and compared between two groups before and after treatment .Results:Compared with before treatment , above hemorheological indexes significantly reduced in both groups after treatment ( P< 0.05 all) ,compared with routine treatment group ,there were significant reductions in high shear whole blood viscosity [ (5.12 ± 0.73) mPa/s vs .(4.47 ± 0.86) mPa/s] ,low shear whole blood viscosity [ (7.36 ± 1.32) mPa/s vs .(6.21 ± 1.03) mPa/s] ,plasma viscosity [ (1.47 ± 0.31) mPa/s vs .(1.31 ± 0.25) mPa/s] ,erythrocyte aggregation index [ (3.53 ± 0.45) vs .(2.93 ± 0.26)] and fibrinogen level [ (2.95 ± 0.28) g/L vs .(2.08 ± 0.24) g/L] in combined treatment group , P< 0.05 all .Total effective rate on ECG in combined treatment group was significantly higher than that of routine treatment group (85.71% vs .57.14% ,P<0.05) .Compared with before treatment ,CRP and TNF‐αlevels significantly reduced in both groups after treatment ( P<0.05 all) ,compared with routine treatment group ,there were significant reductions in levels of CRP [ (6.52 ± 1.61) mg/L vs .(5.18 ± 1.25) mg/L] and TNF‐α [ (283.28 ± 18.28) pg/ml vs .(245.08 ± 12.24) pg/ml] in combined treatment group , P<0.05 all .Conclusion:Danqi soft capsule combined routine treatment can significantly improve hemorheology and reduce related inflamma‐tory factor levels ,possess myocardial protection effect in CHD patients ,which is worth extending .

Objective To study the expression of SARS-associated coronavirus nucleocapsid(N) gene in Pichia pastoris and to obtain recombinant N protein with good biological activity.Methods The gene encoding the full N protein of SARS-CoV was amplified by PCR and cloned into Pichia pastoris expression vector pPICZA.The recombinant expression plasmid pPICZA/N was transformed into X-33,KM71H and GS115. The positive insert transformants were screened,cultured and induced by methanol.The recombinant protein was further purified with Ni affinity chromatography.Antigen activity was detected with anti-N monoclonal antibody,polyclonal antibody and sera from SRAS patients.Results SDS-PAGE and immunological analysis demonstrated that only Pichia pastoris transformants KM71H/pPICZA/N and X-33/pPICZA/N expressed Mr 70 000 fusion protein with special antigenicity.Conclusion SARS-CoV N protein expression in Pichia pastoris has been achieved,establishing the basis for further study of biological and immunological function of N antigen.

Communicable Diseases ; Disease Outbreaks ; Humans ; Infection Control ; Science

Objective In order to explore the mechanism of repetitive transcranial magnetic stimulation rTMS) on neuropathic pain,we observed the effect of different frequencies of rTMS on neuropathic pain and the expression of neuronal nitric oxide synthase (nNOS) in the dorsal root ganglion (DRG).Methods A total of 28 male Sprague-Dawley rats were used and divided into a control group,in which sham-operation was performed,and an experimental group which was further divided into a sham-rTMS group,a 1 Hz group and a 20 Hz group after successful neuropathic pain model was established by operation to ligate the left sciatic nerves,with 7 rats in each group.Three days after the operation,rTMS was applied to the primary motor cortex (M1) contralateral to the pain once dai ly for 10 consecutive days.The pain behavior and nNOS expression in the DRG were measured before and after 10 days of rTMS intervention.Results All the neuropathic pain model rats demonstrated pain-related behaviors 3 days postoperation,the mechanical pain thresholds were significantly lower than those in the control group (P ＜ 0.05).After rTMS treatment,the mechanical hyperalgsia was significantly relieved in 20 Hz group but not 1 Hz group as demonstrated by a comparison with the sham-rTMS group (P ＜ 0.05).The expression of nNOS in DRG ipsilateral to the neuropathic pain was significantly increased in sham-rTMS group and 1 Hz group (P ＜ 0.05) when compared with the control group.Meanwhile,it was shown that expression of nNOS was down-regulated in 20 Hz group but not 1 Hz group (P ＜ 0.05).The degree of pain relief in 20 Hz group was negatively correlated with the expression of nNOS in DRG (P ＜0.05).Conclusions Neuropathic pain induced by peripheral nerve injury is associated with elevated expression of nNOS in the DRG.High-frequency rTMS can relieve neuropathic pain through down-regulating the overexpression of nNOS in the DRG,but the low-frequency rTMS has no such effect.

Stem cells are those with long-term self-renewal and produce differentiated cells at least one end of the cell capacity. Stem cell transplantation can be used for the treatment of many kind of liver diseases which includes acute liver failure, inherited metabolic liver diseases, as well as end-stage liver disease. This Article summarizes the types of stem cell types applied for treatment of liver diseases, stem cell tracing technology, as well as the clinical application of stem cells. It also discusses the problems existed and the research focus.

Objective To investigate the value of multi-slice spiral CT(MSCT)in diagnosis of the causes of intestinal obstruction.Methods 49 cases of intestinal obstruction confirmed by surgery or clinical diagnosis underwent abdominal MSCT plain and enhanced scanning,three-dimensional post-processing reconstruction was done in 5 cases.CT features were analysed blindly by two senior radiologists,including the thickness,density and degree of enhancement of intestinal walls at the "transition zone" area of intestine and surrounding mesenteric structure.The causes of intestinal obstruction were analysed in combination with clinical history of the patients.Results The causes of intestinal obstruction were suggested by MSCT in 46 cases(94%),including intestinal adhesion in 13 cases,tumor in 11,hernia in 4,ischemic bowel disease in 4,gallstone in 2,gastrolith in 2,Bezoar in 4.paralytic ileus in 3 and appendiceal abscess in 3.Three cases were misdiagnosed.Conclusion MSCT is of significant clinical value in diagnosis of intestinal obstruction,which should be used as the first means in clinic practice.

To prepare and study the pharmacokinetics and release bioavailability in olunteers and concentrations in plasma in patients. METHODS: Ethylcellulose was used matrix in phase separation-coacervation for preparation of microencapsulation. The release experiments were performed in a rotating shaker. The isoniazid concentration in plasma was determined by spectrophotometrical method following a single oral dose of sustained-release cupsule and ordinary tablet respectively given to 10 volunteers in a open randomized cross-over test. MCP86 was used to process main pharmacokinetic parameters. RESULTS: The sustained-release of capsule and ordinary teblet in vitro, T50 was 1 h and 0.032 h respectively. The drug in sustained-release capsule was sustained release over 10 h. The main parameters in body: ordinary tablets: cmax=11.12 μgml-1, tmax=1.41 h, K=0.201 h-1; sustained release capsule: cmax=4.99 μgml-1, tmax=1.80 h, K=0.03 h-1. The concentration of blood at 36 h was (0±0)μgml-1 and 1.63 μgml-1 respectively. Except tmax, there was significant difference between the two fomulations (P＜0.01). The concentration of blood in patient at 1.5 h and 36 h. ordinary tablet and sustained-release capsule respectively were (8.24±2.60)μgml-1, (0±0)μgml-1and (3.69±0.86)μgml-1, (2.09±0.56)μgml-1. CONCLUSION: The sustained-release capsule will play an important part in prevention and treatment of tuberculosis as the result of its reasonable formulation and simple technology.

General Policies of Nomenclature for Biological Products in China were studied,aimed at establishing and perfecting the system of Chinese approved biologic name.By means of analysis of the current situation and existing problems of nomenclature for biological products and the development trend of new biotherapeutics.It is sufficiently studied on the existing leading international drug generic naming system,and the specific suggestions on revision of the General Policies of Nomenclature for Biological Products in China based on the naming principles of WHO INN and Chinese language recognition were put forward.

Objective To investigate the clinical feature on pulmonary complications after renal transplantation,in order to provide the evidence of prevention and treatment of pulmonary complications.Methods The clinical data of 125 renal transplant re-cipients in the First Affiliated Hospital of Chongqing Medical University from December 2006 to December 2011 were studied retro-spectively.To analyze and summarize the clinical characteristics of postoperative pulmonary complications including the incidence, the time of onset,etiology or risk factors,treatment,outcome,et al.Results Twenty-three patients(18.4%)developed pulmonary complications after renal transplantation.The most frequent complication was pulmonary infection(n = 22,17.6%),followed by pleural effusion(n=12,9.6%),pulmonary edema(n= 7,5.6%),respiratory tract bleeding(n=4,3.2%),acute lung graft versus host reaction(n=1,0.8%)and atelectasis(n= 1,0.8%).Conclusion Pulmonary infection,pleural effusion and pulmonary edema are common pulmonary complications after renal transplantation.Patients who develop pulmonary infection and pulmonary edema have a poor prognosis.

The proto-oncogene, c-kit gene, widely expresses on the mast cell, melanoeyte, haemopoietic stem cell, intestinal cell of cajal and germoeyte. Recent researches have revealed the relationship between the e-kit gene and the carcinogenesis, proliferation, infiltration and metastasis of some malignant tumor. This article aimed to make a review of its biological function, lab and clinical research advancement in the digestive tract tumor.