Objectives:To investigate the effect and mechanism of adventitia applied slow-releasing triptolide on proliferation and apoptosis of VSMCs of the autologous vein graft. Methods:Twenty-four healthy rabbits were made external jugular vein to common carotid artery models and then were divided into 3 equal groups at random:Blank-control group received no management on adventitia of the vein graft;F-127 pluronic gel control group received local application of 0.5 ml 20% F-127 pluronic gel containing 0.1ml DMSO on adventitia;and experimental group received local application of 0.5 ml F-127 pluronic gel containing triptolide 300?g on adventitia. Vein graft samples were harvested at 2 weeks after the operation. Histomorphologic methods were used to detect the degree of intima hyperplasia of the samples. The expressions of proliferating cell nuclear antigen(PCNA) and bcl-2 of the samples were detected by immunohistochemistry. The apoptosis of vascular smooth muscle cells(VSMC) was detected by TUNEL. Results:Two weeks after vein grafting, compared with blank-control group and F-127 control group,intima hyperplasia was markedly inhibited by applying triptolide 300?g on adventitia(P

Objective To investigate the inhibitory effect of appling slow-releasing triptolide on adventitia on the intima hyperplasia of autologous vein graft.Methods In 24 male New Zealand rabbits, external jugular vein to common carotid artery models were established, and then were divided into 3 equal groups at random: blank-control group, receiving no management on adventitia of the vein graft; F-127 control group, receiving local application of 0.5 mL of 20 % F-127 on adventitia of the vein graft; experiment group, receiving local application of 0.5 mL of 20 % F-127 containing triptolide 300?g. Vein graft specimens were harvested at 2 weeks after the operation. Histomorphologic methods were used to detect the degree of intima hyperplasia of the specimens. The expression of bcl-2 and Fas of the specimens was detected by immunohistochemistry. The apoptosis vascular smooth muscle cells (VSMC) were detected by TUNEL.Results Two weeks after vein grafting, compared to blank-control group and F-127 control group, intima hyperplasia of experiment group [intima thickness (29.9?7.6)?m, I/M 0.56?0.08] was markedly inhibited (P

Yifeng (TE 17), Tinghui (GB 2), Zhongzhu (TE 3) and Xiaxi (GB 43) were selected as key acupoints plus hyperbaric oxygen to treat 50 cases of sudden deafness, and the total effective rate was 98%.

Objective To explore the relationship between subclinical hypothyroidism(SCH)and microvascular complications(diabetic nephrop-athy and diabetic retinopathy)in type 2 diabetic patients. Methods According to the condition of complicating with SCH,157 patients with type 2 diabetes were divided into SCH group(40 cases)and the euthyroid group(117 cases). The age,sex,duration of diabetes,smoking status,blood pressure,body mass index(BMI),glycosylated hemoglobin(HbA1c),blood lipid,renal function,thyroid function,urine albumin/urine creatinine, and fundus examination were analyzed and compared between the two groups. The relationship between SCH and microvascular complications in type 2 diabetic patients was explored. Results There was no significant difference of age,sex,duration of diabetes,smoking,blood pressure,BMI, HbA1c,blood lipid,or serum creatinine between the two groups. The incidence of diabetic retinopathy in SCH group was significantly higher than those in euthyriod group(32.5%vs 14.5%,P=0.044);however there was no difference in the incidence of diabetic nephropathy. After adjustment for potential confounding factors(age,sex,duration,blood pressure,BMI,HbA1c,diabetic nephropathy)by multivariate logistic regression analy-sis,SCH was still the risk factor of diabetic retinopathy(OR=2.210,95%CI 1.512-4.117,P=0.032)in type 2 diabetic patients. Conclusion SCH could be a risk factor of diabetic retinopathy in type 2 diabetic patients,and there was no significant effect on diabetic nephropathy.

Objective To study the clinical effect of psychological intervention combined with oral administration of aminoglucose hydrochloride capsules combined with intra-articular injection of sodium hyaluronate combined with functional exercise in the treatment of knee osteoarthritis.Methods Forty-eight patients with knee osteoarthritis were randomLy divided into control group and study group(n=39).The patients were randomLy divided into control group and study group.The control group was given intraperitoneal injection of sodium hyaluronate combined with functional exercise, the study group on the basis of the control group to give psychological intervention and oral administration of aminoglucose capsules combined therapy.Comparison of two groups of Lysholm knee function score and postoperative efficacy.Results The scores of Lysholm knee function were significantly improved after treatment, but the improvement rate of the study group was significantly better than that of the control group (P<0.05).The clinical effect of the study group was significantly better than that of the control group(94.87% vs 43.59%).The difference was statistically significant (P<0.05).Conclusion The combination of psychological intervention combined with oral administration of aminoglucose capsules with intraperitoneal injection of sodium hyaluronate combined with functional exercise in the treatment of knee osteoarthritis can significantly improve the clinical efficacy, improve the knee function and improve the prognosis, worthy of clinical application and promotion.

Objective To explore the clinical effect of calcium and calcium combined with psychological intervention on elderly patients with osteoporosis and pain.Methods The control group was given routine drug treatment (Miacalcic+Caltrate D), the study group of osteoporosis pain in patients with psychological intervention in the conventional miacacic+Caltrate D treatment on the basis of records of two groups of VAS, SDS, SAS, SF-36 scale score changes (before and after treatment).Results After treatment, two groups of SF-36 scale scores were significantly increased than before, while the VAS scale was significantly lower than before, the quality of life of the study group the degree of pain, improve the effect of better (P<0.05);the research group after treatment SDS, SAS score than before the decline than the control group (P<0.05).Conclusion The application of calcium dense combined with psychological intervention in the treatment of elderly patients with osteoporosis pain is beneficial to achieve more satisfactory pain relief effect, and significantly alleviate the corresponding negative emotions.

The contents and features of medical imaging technology and medical image processing course are introduced in this paper,relationship of the two courses and their different parts are mainly discussed.Contents and the multimedia courseware are then designed.

Objective To determine the role of SCN1A gene variation in the development of familial febrile seizures (FS).Methods Clinical data were collected from 8 familial FS pedigrees, and peripheral venous blood samples were collected from the probands and other available family members. All 26 coding exons and exon-intron boundaries at least 50 bases of the human SCN1A gene were amplifled by polymerase chain reaction, the products were subsequently sequenced. To novo variation, other family members were screened for the corresponding exons. Two hundred age-matched healthy children were served as normal controls. ResultsA total of 33 variations in the SCN1A gene were identifled in these families. Of these variations, one was a missense mutation; the remaining 32 variations were previously submitted as single nucleotide polymorphisms (SNPs). A c.2650G>A heterozygous missense mutation in exon 15 of the SCN1A gene found in the proband of family 4 was inherited from his father who had seizures with fever in early childhood. The c.2650G>A mutation was absent in the 400 alleles of normal controls. To the best of our knowledge, the SCN1A c.2650G>A mutation has neither been reported in the NCBI SNP database nor in the literature to date. The c.2650G>A mutation changes a glycine at amino acid 884 in the SCN1A protein to a serine (p.Gly884Ser). Protein sequence analysis showed that the p.Gly884Ser is located at a highly conserved region between the 4th and 5th transmembrane segment of the homologous domain Ⅱ of voltage-gated sodium channel 1 subunit (DIIS4-S5). ConclusionsThe pathogenesis of familial febrile seizures was related to the SCN1A variation, the mutation outside the region of the voltage sensor (S4) and ion channel pore (S5-S6) of the voltage gated sodium channelα-subunit may be an important factor to cause mild phenotype epilepsy syndrome.

Drawing on recently published foreign literature, this paper comprehensively analyses the research status of medical tourism from the concept of decision-making factors and risks for tourists, as well as the impacts of medical tourism. For future research, it proposes a greater focus on medical tourists, caregiver-companions and medi-cal tourism facilitators. To promote the development of Chinese medical tourism, a development model must be con-structed, international medical services, consummate policies, and regulations must be in place, and medical tourism personnel must be trained and welcomed.

Network theory is applied to the optimization study on field medical equipment allocation with patient flow and its rate in wartime analyzed. With the application of queuing theory and linear programming, different optimized combinations of evacuation tools are studied, and then static model and dynamic model are established. A calculating table on queue length, service intensity and service desk is put forward to realize rapid evaluation of quantities of required field medical equipment for PLA. Some suggestions are provided to modify the current standard of PLA's basic medical equipment.