Objective Cerebral infarction is a main disease threatening human health and the recurrence of cerebral infarction is more inclined to cause disability and death.The article aimed to explore the influence of total sleeping time on the recurrence of cerebral infarction.Methods According to the criteria, 70 cases of cerebral infarction patients who hospitalized in our hospital from March 2016 to November 2016 were selected to undergo polysomnography (PSG) and were divided into two groups according to medical history: original group and recurrence group.Another 20 simultaneous outpatients who had no significant diseases to undergo PSG were chosen as the control group.Data were collected on all the individual''s total sleeping time (TST), gender and age.Hypothesis testing and logistic related analysis were applied in data analysis.Results The TST of recurrence group was shorter than those of the original group and the control group (P<0.05), among which the TST of the original group is shorter than that of the control group (P<0.05), both representing significant difference.Multiple regression analysis showed that TST was an independent risk factor for the recurrence of cerebral infarction and that the risk of recurrence of cerebral infarction increased with the lack of TST.Conclusion The shortening of TST may increase the risk of the recurrence of cerebral infarction.

Adult ; Biomarkers, Tumor ; metabolism ; Calbindin 2 ; Female ; Follow-Up Studies ; Humans ; Mesothelioma ; metabolism ; pathology ; surgery ; Mucin-1 ; metabolism ; Peritoneal Neoplasms ; metabolism ; pathology ; surgery ; S100 Calcium Binding Protein G ; metabolism

OBJECTIVE:To compare the effects of omeprazole and esomeprazole,two kinds of proton pump inhibitors,on clopidogrel platelet inhibition following cardiac stents implantation.METHODS:Totally 180 patients with coronary artery disease underwent percutaneous coronary intervention (PCI) at the Zhujiang Hospital of Southern Medical University from June 2008 to May 2009 were selected,including 83 males and 97 females.All patients were randomly divided into 3 groups,omeprazole + clopidogrel + aspirin group (OCA group,receiving omerprazole 20 mg/d),esomeprazole+clopidogrel+aspirin group (ECA group,receiving esomeprazole 10 mg/d),and control group (No proton pump inhibitor),with 60 patients in each group.In addition,all patients received a 300 mg clopidogrel and 0.1 g aspirin prior to PCI,and received 75 mg/d clopidogrel and 100 mg/d aspirin treatment for 1 week after PCI.Blood samples from patients were obtained from cubital vein before and at 1 week after adminstration,respectively.The vasodilator stimulated phosphoprotein phosphorylation state and platelet reactivity index (PRI) were calculated by flow cytometric assay.RESULTS:The PRI had no significant difference before administration (P>0.05),which was obviously decreased at 1 week after administration (P<0.05),especially lowest in the control and ECA groups (P<0.05).However,the PRI differences between the control and the ECA group had no significant (P>0.05).CONCLUSION:The administration of omeprazole rather than esomeprazole is associated with impaired clopidogrel platelet inhibition.Esomeprazole can be used as one of the preferred proton pump inhibitor in curing gastrointestinal bleeding caused by anti-platelet therapy following cardiac stents implantation.

Ischemic cerebrovascular disease is one of the major reasons of mortality and disability in adult populations.Ultra-early recanalization is by far the most positive treatment.Relative to intravenous thrombolysis,various endovascular treatment modalities can extend therapeutic time window and increase the recanalization rate.Furthermore,interventional therapy can also improve the clinical outcomes of patients by increasing the perfusion of colhteral circulation.This article reviews various endovascular treatment modalities and their application validated in previous clinical studies in China.

Objective In order to find the best methods for treatment of lumbar intervertebral disc herniation through posterior approach microendoscopic discectomy. Methods The approach of operation by lamina arcus vertebrae space was used to remove ligamentum flavum not to removed lamina arcus vertebrae and other tissue in lateral recumbent position. Extraction of lumbar interverebral disc herniation was used in 113 cases. Results A follow-up study was performed after operation, ranged from 6 to 24 months with a mean duration of 12 months. According to Nakai standard, there were 107 cases scored "excellent" and 6 scored "good". Conclusion Microendoscopic discectomy by the approach of lamina arcus vertebrae space caused less injury and bleeding, and was safe for both surgery and anesthesia. It achieved successfully without affecting the spinal stability.

Objective： To investigate the modification of GABAA receptor subunits in audiogenic seizure rat cortex and hippocampus when rendered tolerant to flurazepam. Methods： Rats were administrated with flurazepam for two weeks, which resulted in tolerance to flurazepam, in the absence of behavioral signs of withdrawal. GABAA receptor subunits α1, α3, α5, γ2L and γ2S were assayed using quantitative competitive RT-PCR in rat frontparietal motor(FrPaM) and hippocampus. Results： In FrPaM, the contents of mRNA encoding for α1, α3,　γ2L and γ2S were all significantly decreased (24%,17%,35% and 45% respectively),whereas that of α5 was significantly increased (33%) compared with the control. In hippocampus, α1, γ2L, and γ2S mRNA contents were significantly decreased (33%, 35%and 27% respectively). Conclusion： The accomodated change in GABAA receptor subunits α1, α3, α5,γ2L and γ2S in FrPaM and hippocampus may be associated with the mechanism of flurazepam tolerance in audiogenic seizure rats.

[Objective] To explore the reasons of catheter-related infections in ICU critical pa-tients and raise up the countermeasures.[Methods] The clinical data of 46 patients with catheter-related infections were surveyed and analyzed from May 2010 to March 2011 in our hospital.[Results] Elderly patients occupied the majority of ICU patients with catheter-related infections.The main infection sites included the respiratory system,urinary system and gastrointestinal system.Establishment of artificial airway was the main cause of infection,indwelling catheter came next.[Conclusions] ICU patients has a high incidence of catheter-related infections.We should handle the ICU management appropriately,strengthen the control and prevention of factors of catheter-related infections,strictly enforce disinfection and segregation regulation,cut off infection way,enhances the resistance of patients,limit antibiotic use and various invasive operation in order to reduce the incidence of catheter-related infections.

AIM To investigate the modification of GABA A receptor subunits in audiogenic seizure rat cortex and hippocampus when rendered tolerant to clobazam. METHODS Rats were administrated with clobazam for two weeks, resulting in tolerance to clobazam. GABA A receptor subunit ?1, ?3, ?5, ?2L and ?2S were assayed using quantitative competitive RT PCR in rat FrPaM and hippocampus. RESULTS In FrPaM,the content of mRNA encoding for ?1,?2L and ?2S were all significantly decreased (27%,43% and 37% respectively),whereas that of ?5 was significantly increased (73%) compared with the control. In hippocampus, ?1, ?2L, and ?2S mRNA content was significantly decreased (28%, 32% and 31% respectively). CONCLUSION The accomodated change in GABA A receptor subunit ?1, ?5,?2L and ?2S in FrPaM and hippocampus may be associated with the mechanism for tolerance to clobazam in audiogenic seizure rat.

Objective To understand the content of fluoride in dinking water in the rural area in Yaodu district and to provide the data for water improvement. Methods 873 water samples were randomly collected from 175 villages in Yaodu district during Apr.-Sep., 2006 and the fluoride ion selective electrode was used to determine the fluoride in dinking water. Results The eligible rate of fluoride concentration in the investigated drinking water samples was 54.75%. The range of fluoride concentration was 0.24-5.44 mg/L. Conclusion The investigated area is the moderate or severe prevalent area of endemic fluorosis, water improvement should be enhanced in this area.

Background and purpose:Hsp90 is cell chaperone protein that interacts with many proteins,but itself can not degrade its client proteins.Hsp90 inhibitor can inhibit tumor cell proliferation,induce cell apoptosis,cell growth arrest and increase the degradation of Hsp90 client proteins like Survivin.In order to explore the co-effects of Hsp90 inhibitor 17-AAG and Survivin on LoVo cells and the possible mechanisms,we observed the effects of 17-AAG on proliferation and cycles of LoVo cells and the protein level of Survivin.Methods:LoVo cells were treated with 17-AAG.The cell proliferation inhibition rate was evaluated by MTT assay.The cell cycle was detected by ? ow cytometry.The expression of Hsp90 client protein Survivin was detected by Western blot.Results:17-AAG time-dose-dependently inhibit the proliferation of LoVo cells,after 100 ng/ml,500 ng/ml and 800 ng/ml 17-AAG exposure for 24 hrs,the cell proliferation inhibition rate was 21.00%,40.81%,60.34% respectively,after exposure for 48 hrs,the cell proliferation inhibition rate was increased to 27.29%,48.17%,80.97% respectively,after exposure for 72 hrs,the cell proliferation inhibition rate was to 34.45%,67.81%,88.42%;17-AAG arrested cell cycle,when LoVo cells were exposed to 100 ng/ml 17-AAG for 72 hrs,the cell ratio of G0/G1 phase was(61?3)%,when to 500 ng/ml for 72 hrs,cell ratio of G0/G1 phase was increased to(74?3)%,compared to(48.2?0.8)% LoVo cells without 17-AAG(P