Objective:To explore application value of real-time three-dimensional echocardiography (RT-3DE ) for heart function evaluation after percutaneous coronary intervention (PCI) .Methods:Clinical data of 45 patients with coronary heart disease (CHD) collected from Jan 2013 to Jan 2014 in our hospital were enrolled .All patients received selective coro-nary angiography (CAG) and PCI .Another 45 healthy cases undergoing physical examination during the same period were selected as normal control group .End-diastolic volume (EDV) ,end-systolic volume (ESV) ,left ventricular ejection frac-tion (LVEF) ,peak ejection rate (PER) and peak filling rate (PFR) were measured by RT-3DE and two-dimensional echo-cardiography (2DE) respectively and compared between two groups .Results:In normal control group ,there were no sig-nificant differences in all indexes between two measuring methods ,P>0.05 all;in CHD group ,compared with 2DE meas-ured value ,there were significant rise in EDV [ (130.6 ± 30.3) ml vs .(152.5 ± 35.2) ml] ,ESV [ (75.2 ± 21.8) ml vs . (94.1 ± 22.5) ml] ,PER [(2.41 ± 0.47) vs .(2.81 ± 0.39)] and PFR [(1.85 ± 0.25) vs .(2.13 ± 0.31)] ,and significant reduction in LVEF [ (45.8 ± 5.2)% vs .(41.6 ± 4.9)% ] in RT-3DE ,P<0.01 all;on one week ,one month and three months after PCI ,EDV and ESV gradually reduced ,LVEF ,PER and PFR gradually rose in CHD group with significant difference ,P<0.05 or <0.01 .Conclusion:RT-3DE can perform direct ,accurate and quantitative evaluation on heart form and function before and after PCI ,which is a reliable ,fast ,simple and new method evaluating PCI therapeutic effect in CHD patients .

BACKGROUND:Extracorporeal cardiac shock wave therapy (CSWT) can up-regulate angiogenic factors and its receptors,promote angiogenesis and collateral circulation establishment by introducing shock waves to myocardium,however,the specific mechanisms remain unclearly.OBJECTIVE:To observe the influence of CSWT on the content of serum endothelial nitric oxide synthase (eNOS) in pigs following acute myocardial infarction (AMI).METHODS:Pig AMI model was established by embolizing the distal of left anterior coronary artery.Following model preparation,pigs were randomly divided into 2 groups.In the experimental group received three CSWT at days 1,3 and 5,200 hits per point,totally 12 points,with 0.09 mJ/mm~2 energy.Pigs with simple AMI were served as the control group.The serum eNOS level was detected by ELISA method before,immediate postoperative,and at days,1,3,5,and weeks 1,2,3,and 4 after operation.RESULTS AND CONCLUSION:The eNOS level of both groups were obvious decreased at immediate postoperative than that before operation.At day 1 after CSWT,eNOS level was increased in the experimental group,which was still decreased in the control group.It revealed that CSWT play a positive effect on ischemia rnyocardium.The eNOS level reached a peak in the experimental group after 3 times of CSWT,lasted for 2-3 weeks,and began to decrease at week 4.The difference between the experimental group and control group had significance (P＜0.01).The results demonstrated that CSWT can treat AMI effectively by promoting eNOS expression.

BACKGROUND: Coronary occlusion and balloon occlusion are two major methods in preparing acute myocardium infarction models. The pigs were expensive and the modeis will periorm further study,therefore,it is necessary to prepave a model with advantage of short time consuming, few trauma, and high survival rate.OBJECTIVE: To establish acute myocardium infarction in miniature pigs induced by coronary occlusion and balloon occlusion,and to compare the differences.DESIGN, TIME AND SETTING: The randomized grouping, contrast observation animal experiment was performed at the heart intervention room of The First Affiliated Hospital of Kun Ming Medical College between May 8~(th) and August 3~(rd), 2009.MATERIALS: A total of 20 Banna miniature pigs, weighing 20-30 kg, irrespective of genders, were purchased from animal department of Kunming Medical College. The animals were equally divided into embolism and occlusion groups.METHODS: In the embolism group, the balloon was implanted into distal end of anterior descending coronary via femoral artery,blocked the forward flow with 202.65 kPa pressure for 1, 2, and 5 minutes, with 60 seconds interval. The same operation was performed in the occlusion group, followed by blocked the forward flow for successive 60 minutes.MAIN OUTCOME MEASURES: Differences of modes established by 2 methods were compared by coronary opacification, ECG,myocardium zymogram, ultrasonic cardiogram and pathology detection.RESULTS: Nine pigs were survived in the embolism group, and the remained 1 pig died of coronary artery rupture. After operation, T wave inversion could be found, without ST stage raise. At day 1 after operation, correlated lead ST stage was raised 0.1-0.2 mV. The operation duration was 11 hours. In the occlusion group, 5 pigs survived, 4 pigs died of ventricular fibrillation,and 1 died without definite cause. The operation cost 24 hours, and the correlated lead ST stage was increased 0.2-0.3 mV postoperatively. Motion abnormality in infarction place was exhibited in both groups; ventricular aneurysm was formed in 3 pigs in the embolism group, and 1 in the occlusion group. Levels of creatine kinase isoenzymes serum troponin T were obviously stepped up and presented dynamic changes. The myocardiolysis was obviously showed in the infarcted areas, but the differences were no obviously in 2 groups.CONCLUSION: Both coronary occlusion and balloon occlusion can induce models of acute myocardium infarction. The coronary occlusion method is superior to balloon occlusion with few trauma, faster postoperative recover and low mortality rates.

Animals ; Bilirubin ; toxicity ; Brain ; drug effects ; metabolism ; Nitric Oxide ; metabolism ; Rabbits

[Objective]To evaluate the outcome of the Pemberton osteotomy for the treatment of developmental dysplasia of the hip (DDH) in older children. [Method]There were 21 children (24 hips) older than 7 years in our study , who received the Pemberton osteotomy, and combined with femoral shortening, derotational or varus osteotomies according to the level of dislocation, anteversion and neck-shalf angle of the femur. The average age at operation were 9 years and 7 months (range: 7~13.5 years).[Result]The mean follow-up were 5 years and 5 months. Eighteen hips achieved the excellent or good clinical results according to the McKay's clinical criteria. All of the hips got improvements of the Acetabular Index, Sharp Acetabular Angle, Center-Edge Angle and Acetabular Head Index. The average results were in the normal range. Eighteen hips got ⅠorⅡclass of the Severin’s radiographic classification.However, 9 hips were limited in the range of movement. Four hips got subluxation.Six hips developed avascular necrosis of the femoral head. Three hips got improved osteoarthritis.[Conclusion]The Pemberton osteotomy for the treatment of developmental dysplasia of the hip in older children can mostly achieve well-used, contained or concentric reduction hips, meanwhile need to reduce the complications.

Objective:To study the difference of upper airway form and hyoid position of the adults with different vertical facial types.Methods:Cephalograms of 120 adults with different vertical facial types were retrospectively investigated by computerized cephalometric analysis.Upper airway depths and hyoid position were measured,and differences were compared between male and female,also compared among different vertical facial type groups.Results:The sagittal depth of upper airway in male was larger than that in female,but there was no statistical difference except V-LPW.Among different vertical facial types,the sagittal depths of upper airway in high angle group was smaller than that in average angle group and low angle group.There was statistical difference in hyoid position between genders,AH-SN,AH-FH and AH-C3 in male were larger than in female(P

Objective To explore the immunologic regulation of the bifidobacterium adolescence cell wall(BCW) on function of macrophage in mice and tumor inhibition of BCW on human transitional cell carcinoma of bladder(BTCC) outside of body.Methods BCW wereinjected into abdomen of KM mice,the level of IL-1,IL12 and TNF-?which produced by macrophage was determined by ELISA reagent box.The role of tumor inhibition was determined by MTT method.Results The level of IL-1,IL12 and TNF-?of BCW injection group was higher than of control group(P

Objective To prepare an internal quality control substance of free hemoglobin (FHb),and evaluate its perform-ance.Methods ① 5 normal whole human blood samples with known hemoglobin (Hb)concentration was selected and dilu-ted with distilled water in the ratio of 1∶500,1∶1 000,1∶2 000,1∶4 000 respectively.After 20 FHb solutions was fully mixed,the concentration of FHb solutions was tested and compared with its theoretical data.②One of the highest value and the lowest value solutions were chosen as qualitye control substance,packed and stored in -20℃ fridge.Each group was an-alyzed 20 days continuously in the routine conditions,where average (-x )of the groups,standards deviation (s )and coeffi-cients of variation (CV)were calculated and Levey-Jennings control chart was conducted.Then,the test was repeated every week,and the data on the chart was updated accordingly until (CV)of the reagents were changed.The average (-x)of the-groups,standards deviation (s)and coefficients of variation uality control material was calculated during the whole test peri-od.Results ①The theoretical value (-x ±s)of the concentration of free hemoglobin was 125.44±93.04 mg/L,and the actu-al value was 125.22±93.08 mg/L,there was no statistic significance (t value was 0.706,P >0.05).② During 20 continu-ously daystest,the detected values (-x ±s)of the highest quality control material was 303.55±3.70 mg/L;the values of the lowest quality control material was 69.29 ± 1.88 mg/L.The coefficients were 1.22% and 2.68% respectively.Both the highest and the lowest quality control material were under control during the whole detecting period.The (-x ±s )values of the highest was 302.56±3.99 mg/L,the CV value of it was 1.32%;The (-x ±s)values of the lowest was 69.04±1.88 mg/L,the CV value of it was 2.71%.Conclusion The preparation method of internal quality control substance of free hemoglo-bin is convenient and stable,and was applicable in blood station’s and clinical laboratory.

Huichunzhibao oral liquid (HZOL ) is a traditional Chinese herb Preparation composedof Panax ginseng, Hairy Antler (Cervus nippon Tcmminck ) and Epimedium brevicornum Maixm..The active principle of each component was identified by TLC and icariin, the main active principle of E. brevicornum was determined quantitatively by HPLC. The meth od was found to be accuratc, sensitive and reproduciblc with average recovery 98.97 % andRSD = 1.53 (n = 3).