Objective]To discuss and analyze Professor Cheng Jing's clinical experience for treating decline in ovarian reserve(DOR). [Method]Through learning from the tutor in the outpatient service and case studies ,we analyzed and summarized Pro. Cheng's recognition on the etiology and pathogenesis of DOR,together with his dialectical way of thinking, combined therapy of Chinese and western medicine ,and empirical prescriptions. [Result] Pro. Cheng believes that this disease lies in the kidneys as wel as in the thoroughfare and conception meridian ,the pathogenesis is kidney deficiency and the imbalance of the thoroughfare and conception meridian,or combined with the stagnation of liver qi,spleen deficiency,and blood stasis and phlegm fire (heat); the therapy is mainly nourishing and warming kidneys as wel as conditioning the thoroughfare and conception meridian ,combined with Chinese medicine's periodical therapy,such as adjusting the circulation of qi and blood in livers,spleens,hearts and other organs. [Conclusions] Pro. Cheng's combined therapy of Chinese and western medicine for curing DOR ,of which his empirical prescriptions the Bushenyichongkangshuaitang is the main part,is of remarkable curative effect and significant clinical value.

Objective To evaluate the effect of group reminiscence therapy on improving the depressive symptoms of the institutionalized elderly. Methods Collecting clinical randomized controlled trials and quasi-randomized controlled trials of group reminiscence therapy for improving depressive symptoms in institutionalized elderly which published from 2000 to November 2016, by searching the Cochrane Library, PubMed, CINAHL, PsycINFO, EMbase, China Biology Medicine, China National Knowledge Infrastructure, VIP, Wanfang Database, Taiwan Academic Online Database and Airiti Library. And reviewing the quality of the studies by using the tools for assessing risk of bias. RevMan 5.3 software was used for data analysis. Results Ten studies(2 randomized controlled trials and 8 quasi-experiments), including a total of 511 cases, were included. The results showed that group reminiscence therapy significantly improved the depression, life satisfaction, and self-esteem of institutionalized elderly (95%CI-1.11--0.56, 0.41-2.05, 1.99-3.82, all P<0.01). There was no statistically significant difference between group reminiscence therapy and usual nursing in relieving loneliness (P = 0.21). Conclusions Group reminiscence therapy can improve the depression in institutionalized elderly, decrease the degree of depression, improve the level of life satisfaction and self-esteem. But in the ease of loneliness has not been confirmed. The randomized controlled trials included in this study were rare and the quality of the literature was low, and large-scale randomized controlled trials of higher quality are needed to confirm this.

In recent years ,researches suggested that inflammation is closely related to occurrence and development of heart failure .Some studies have indicated that high sensitive C reactive protein (hsCRP) and neutrophil -lym‐phocyte ratio (NLR) were independent predictors for prognosis of heart failure ,so it is new targets for heart failure treatment .The present article made a review for new progress of related research .

OBJECTIVE:To study the effects of baicalin on human osteosarcoma MG63 cells apoptosis and the expression of MMPs. METHODS:Treated with 0(blank control),5,10,20,40,80,160,320 μg/ml baicalin for 24 h,the survival rate,the expression amount of MMP-2 and MMP-9 were detected,and IC50 was calculated. Cell apoptosis was observed. RESULTS:Com-pared with blank control group,after treated with baicalin,survival rate of MG63 cells decreased,while apoptotic amount in-creased,the expression amount of MMP-2 and MMP-9 decreased;there was statistical significance in the expression amount de-crease of MMP-2 and MMP-9 in MG63 cells after treated with 320 μg/ml baicalin(P<0.05);IC50 was(40.21±9.20)μg/ml,all responses were in concentration-dependent manner. CONCLUSIONS:Baicalin can inhibit the proliferation of MG63 cells,induce cell apoptosis,and inhibit the expression of MMP-2 and MMP-9 in cells under high concentration.

BACKGROUND:Internal fixation has been shown to decrease intertrochanteric fracture complications in the clinic and to improve quality of life of patients. The major method widely used in the clinic is the fixation of proximal femoral nail and dynamic hip screw. ＜br> OBJECTIVE:To observe the clinical effects of proximal femoral nail and dynamic hip screw in treatment of intertrochanteric fracture in the elderly. ＜br> METHODS:A total of 60 patients with intertrochanteric fracture were randomly divided into proximal femoral nail and dynamic hip screw groups (n=30), and they respectively received proximal femoral nail and dynamic hip screw fixation. ＜br> RESULTS AND CONCLUSION:Compared with the dynamic hip screw group, operative time, blood loss, the incidence of complications one year after repair, fracture healing time and complications were lower in the proximal femoral nail group (P＜0.05). After repair, the excellent and good rate of Harris hip scores was higher (P＜0.05). No significant difference was detected in healing time of wound between the two groups (P>0.05). Results verified that compared with the dynamic hip screw fixation, the clinical effects of proximal femoral nail fixation were better, more safe and reliable in the treatment of intertrochanteric fracture in the elderly.

Objective: To compare the role in recognizing mild cognitive impairment of visual logical memory to auditory logical memory. Methods: 41 normal aged controls and 41 patients with mild cognitive impairment(MCI) were required to finish immediate recall and delay recall by visual and auditory logical memory test. Results: There was significant difference in immediate recall and delay recall between the two groups who received visual and auditory logical memory test, and the LM of visual logical memory test was superior to that of auditory one. Auditory logical memory-II could recognize more MCI individuals than visual logical memory-II. The accuracy of recognizing MCI by visual logical memory test was 88% when we set the standard score at 5 points, and 93% for auditory logical memory test with the standard score at 3 points. Conclusion: Delay recall of auditory logical memory test is the most sensitive one among all the logical memory tests for recognizing MCI.

Objective: To probe the significance of spontaneous and mimicking clock drawing tests (CDT) to evaluate the severity of Alzheimer’s disease (AD). Methods: 30 normal middle aged to senile people as the control, and 20 amnestic mild cognitive impairment patients, 20 mild AD patients, 19 moderate AD patients and 14 severe AD patients were asked to finish the series of neuropsychologic test including the spontaneous and mimicking clock drawing tests. Results: For spontaneous CDT, there was significant difference in the "anchoring part"(draw the four key points-12-3-69 first) between the MCI patients and normal controls (P

Objective To observe the distribution of parvalbumin immunoreactive neurons in the hippocampal formation of human fetuses. Methods The distribution of parvalbumin immunoreactive neurons in the hippocampal formation of human fetuses at 30 week were investigated by ABC immunocytochemical technique. Results The abundant parvalbumin immunoreactive neurons were localized in all regions of hippocampal formation, especially in the stratum pyramidale. In the stratum oriens of CA1\|3, the triangle or shuttle parvalbumin immunoreactive neurons with some processes were smaller and scattered in this layer. In the stratum pyramidale, the parvalbumin immunoreactive neurons distributed densely, and the processes run out to the stratum oriens and stratum moleculare. In the stratum moleculare the parvalbumin immunoreactive neurons were sparser than in the stratum oriens and stratum moleculare. The parvalbumin immunoreactive neurons in hilus were denser in the hippocampal formation, compared with the other regions, however, delamination of them was not distinct. The processes of parvalbumin immunoreactive neurons run out to the dentate gyrus. The parvalbumin immunoreactive neurons in the dentate gyrus were mainly localized in the stratum granule. Few immunoreactive neurons distributed in the other layers, which were lightly stained and with no processes. In the subicular complex, the lightly stained neurons with few processes were mainly localized in the stratum pyramidale. Conclusion The abundant parvalbumin immunoreactive neurons presented in all regions of the hippocampal formation, especially in the stratum pyramidale. However, the ripe time of these neurons might be different, neurons in CA3 and hilus were earlier than in the dentate gyrus and subicular complex.

Objective To determine the sensitization of Nicotinamide (NA) on None Focused Ultrasound (NFU) on GLC-82 cells in vitro. Methods The subjectives were divided into A、B、C and D four groups, group A was negative control, group B was treated with NA, group C was irradiated with NFU, group D was treated with both NFU and NA; MTT assay was used to evaluate the cytotoxicity of NA and its sensitivity for NFU on GLC-82 cells quantified by calculating the sensitive enhancement ratios (SER); Morphologic change of cells was observed with light microscope, fluorescence microscope and scanning electron microscope;Flow cytometry was performed to determine the percentage changes of apoptosis and distributional percentage of the cell cycle of GLC-82 cells in response to NFU, NA and the effect of its intervention to NFU. Results The cytotoxicity of NA increased in a dose-dependent manner after 24-hour treatment, with the optimal dose range of 1 mg/mL～5 mg/mL. A sub-toxic dose of NA at 3 mg/ml was used in the subsequent experiments; After treatment with NA plus NFU, microvilli and filipodium of GLC-82 cells reduced, shortened and shrunk into corpuscule-shape; The apoptosis rate of group D was more than that in group B and C, with cell cycle arrested at phase S and G2.Conclusion NA can significantly enhance the effect of NFU on GLC-82 cells, which provides evidence for NA as a sensitization to NFU in clinic.

OBJECTIVE To give an overview of our hospital′s actual infection situation and to draw attention to infection control,thus improving the weaknesses. METHODS With a method of transversal section,choosing the day of Jun 14 2005,we investigated all this day′s hospitalized patients. RESULTS The result was that the infection rate was 6.6%,mostly contributed by the lower respiratory infection. CONCLUSIONS The incidence of nosocomial infection is connected with following factors such as hospitalized time,age,surrounding contamination,and the long time usage of antibiotics.