1.Expressions and Clinical Significance of PTEN,Fas/FasL and MMP-2 in Gastric Cancer
Peng WANG ; Lin GUO ; Qiang WANG
Chinese Journal of Bases and Clinics in General Surgery 2008;0(12):-
Objective To study the expressions of phosphatese and tensin homolog deletedin chromosom ten (PTEN),Fas/FasL system and matrix metalloproteinnases-2 (MMP-2) in human gastric cancer.Methods Seventy-five cases of gastric carcinoma were selected from paraffin wax embodied specimens with full clinicopathological data,and another 15 cases of normal gastric mucosa specimens were selected as the control group.SP immunohistochemistry was used to measure the expressions of PTEN,Fas/FasL and MMP-2 in them.The data was statistically analyzed by ?2 test and relative analysis.Results The expressions of PTEN,Fas/FasL and MMP-2 were correlated with the lymphatic metastasis,degree of infiltration,clinical TMN stage and pathological histological differentiated degree of gastric cancer (P
2.Expression of HIF-1α and survivin in gastric cancer
Wei SUN ; Yu ZHAO ; Lin GUO ; Qiang WANG
Clinical Medicine of China 2008;24(9):885-888
Objective To study the expression of HIF-1α and survivin in gastric cancer,and to study their role in tumor angiogenesis,invasion and metastasis.Methods Immunohistochemieal technique was used to detect the expression of HIF-1α and survivin in 54 cases of gastric cancer.Results The expression rate of HIF-lα in gastric cancer (74.1%) was higher than that in normal gastric tissue (O),The expression of HIF-1α in gastric cancer was associated with TNM staging,invasive depth and lymph-node metastasis (P<0.05),The expression of survivin in gastric cancers was high (72.2%),and increased with invasive depth (P<0.01),clinical stages (P<0.01) and lymphatic metastasis (P<0.05).There was a positive correlation between expression of HIF-1α and Survivin (r:0.31,P<0.05).Conclusion HIF-1α and Survivin could be factors in the judgement of the degree of malignancy of gastric cancer and prediction of its prognosis.
3.Expression of CXC chemokine receptor 4 in Barrett esophagus, esophageal adenocarcinoma and esophageal squamous cell carcinoma and its clinical significance
Chao ZHANG ; Hui TANG ; Lin LI ; Xinmin YAN ; Qiang GUO
Chinese Journal of Digestive Endoscopy 2010;27(11):589-593
Objective To investigate the expressions of CXCR4 in Barrett esophagus (BE), esophageal adenocarcinoma (EADC) and esophageal squamous cell carcinoma (ESCC), and its relationship with pathology, clinical staging and lymph node metastasis. Methods The expressions of CXCR4 in 56 cases of normal esophageal mucosa, 80 BE (including 22 BE with multifocal dysplasia), 25 EADC and 48 ESCC were examined with immunohistochemical method. Results CXCR4 was expressed in most samples of BE (80. 8% ), EADC (68. 0% ) and ESCC (78.4%) without significant difference ( P > 0. 05 ), which was significantly higher than that in normal esophageal mucosa (39. 3%, P <0. 01 ). The level of CXCR4 expression in BE, EADC or ESCC were not related with gender, age, or location of the foci ( P > 0. 05). There was no significant difference in CXCR4 expression between BE without dysplasia or BE with multifocal dysplasia ( P > 0. 05 ). CXCR4 expression level in well-differentiated EADC was significantly higher than that of mild or poorly differentiated (P < 0. 05 ). CXCR4 expression level was higher in EADC with lymph node metastasis than those without ( P < 0. 05 ). CXCR4 level in ESCC with TNM staging grades Ⅲ -Ⅳ was higher than that of grades Ⅰ - Ⅱ, and this variable was also higher in cases with lymph node metastasis than those without (P < 0. 05), so was the case of well and poorly differentiated ESCC (P < 0. 01 ). Conclusion Increased expression level of CXCR4 may be a common feature of EADC and ESCC, which is irrelevant to pathological types. CXCR4 level rises at the stage of BE, which is associated with the degree of tumor differentiation, lymph node metastasis and TNM staging. CXCR4 expression is of guiding significance in the diagnosis of BE, EADC and ESCC, and is the potential drug target.
4.Clinical application of MSCT virtual endoscopy in biliary pancreatic junction
Zhijun GUO ; Yanfang CHEN ; Yuhuan ZHANG ; Fanjie MENG ; Qiang LIN
Chinese Journal of Hepatobiliary Surgery 2011;17(1):10-12
Objective To determine the clinical value of the multi-slice spiral CT virtual endoscopy (CTVE) for the detection of biliary pancreatic junction lesions. Method MSCT and virtual endoscopic reconstruction were performed in 30 healthy volunteers, 18 cases of common bile duct stones and 7 cases of ampullary carcinoma to observe patterns of duodenal papilla and measure its size.Results Reconstructed image of CTVE showed that the normal duodenal papilla was nodular in 16,shaped like "V" in 8 "Y-shaped" in 6 of the healthy volunteers. Its diameter was (0.84±0.17)cm. In the patients with common bile duct stones, it was nodular and its diameter (1.72±0.32)cm. In the patients with ampullary cancer, it was of irregular protruded type and its diameter (2.30±0.85)cm.There was significant difference among the 3 groups in the overall mean values (P<0.01). Conclusion CTVE is a convenient, non-invasive and precise clinical examination to observe the shape of duodenal papilla and determine its size.
5.Perspectives on clinical studies of cancers of the esophagus and gastric cardia from the point of view of high incidence field research.
Guo-Qing WANG ; Wen-qiang WEI ; You-lin QIAO
Chinese Journal of Oncology 2006;28(11):879-880
Cardia
;
pathology
;
surgery
;
China
;
epidemiology
;
Esophageal Neoplasms
;
diagnosis
;
epidemiology
;
surgery
;
Esophagectomy
;
Esophagoscopy
;
Gastroscopy
;
Humans
;
Mass Screening
;
Minimally Invasive Surgical Procedures
;
Precancerous Conditions
;
diagnosis
;
surgery
;
Stomach Neoplasms
;
diagnosis
;
epidemiology
;
surgery
6.Effects of Xuebijing on the expression of ICAM-1 and its counterreceptor LFA-1 in acute necrotizing pancreatitis of rats
Wei SUN ; Fang WEI ; Lin GUO ; Qiang WANG ;
Chinese Journal of Primary Medicine and Pharmacy 2006;0(12):-
Objective To investigate the effects of Xuebijing injection on the production of ICAM-1 and its counterreceptor(LFA-1)in ANP of rats at different time points.Methods ANP was induced by multiple points in- jection of 5% chenodeoxycholic acid into the pancreas,followed by ligation of pancreatic duct.Expressions of ICAM- 1 at different time points were assessed by immunohistochemistry method,the expressions of LAF-1 on neutrophils at different time points were detected by flow cytometer.The effect of Xuebijing injection was also evaluated by de- tecting the output of ascitic fluid,amylase level and histological changes in pancreas and lungs.Results The expres- sions of ICAM-1 in pancreas and lung of Xuebijing injection group decreased significantly than those in ANP group at most time points during the research period(P
7.Clinical study on change of chemokine mRNA and protein in patients with diabetes mellitus complicated infection
Jian GUO ; Da-Lin SONG ; Wei-Qiang KANG ;
Chinese Journal of Laboratory Medicine 2003;0(10):-
Objective To investigate change of chemokine mRNA and protein in the patients with diabetes mellitus(DM)complicated infection.Methods Fourty-eight patients with DM complicated infection were studied.Expression of mRNA and protein of monocyte chemoattractant protein-1(MCP-1)and interleukin 8 (IL-8)were measured by FQ-PCR,RT-PCR and ELISA.The relationship between hs-CRP,PMNL,IL-8 and MCP-1 were analyzed.Results Expression of mRNA and protein of MCP-1 and IL-8 were increased in the DM patients complicated infection.MCP-1 mRNA(mesured by FQ-PCR)was positively correlated with the protein(IL-8 r = 0.33;MCP-1 r = 0.88;P
8.Changes in NT-proBNP after physical training in patients with chronic heart failure
Zhao-Qiang DONG ; Qing-Hua LU ; Jing GUO ; Lin HAO ;
Chinese Journal of Physical Medicine and Rehabilitation 2003;0(07):-
Objective To investigate the effect of physical training on plasma N-terminal pro-brain natri- uretic peptide(NT-proBNP)levels in patients with chronic heart failure(CHF).Methods Eighty NYHAⅡ-ⅢCHF patients were randomly divided into a training group(n=42)and a control group(n=38).A 6-minute walk- ing test was performed within 24 hours after the patients were admitted.The 6-minute walking distance and plasma NT-proBNP levels were determined before and after 8 weeks of programmed physical training.The patients of both groups were treated with routine drugs for heart failure.6-minute walk training was only performed in the training group twice a day for 8 weeks.Results Physical training could significantly reduce plasma NT-proBNP levels and improve performance on the 6-minute walking test.Conclusions Physical training could significantly reduce plas- ma NT-proBNP levels and improve the motor function of patients with CHF,and could be helpful in delaying the de- velopment of CHF.
9.Study on the Biochemical Mechanism of Degrading Keratins by Streptomyces fradiae
Lin HUANG ; Zhi-Qiang XIONG ; Hua-Jing CAI ; Mei-Jin GUO ; Guo-Quan TU ;
Microbiology 1992;0(04):-
The biochemical mechanism of degrading keratins by S.fradiae var S-221 was primarily studied.The compounds (Na_ 2 SO_ 4 , Na_ 2 SO_ 3 and sulfdryl acohol), which respecitively enhance specific activity of keratinase, activate keratinase intensively and mainly act on the disulfide bonds reductase in the keratinase, Na_ 2 SO_ 3 activates intensively both disulfide bonds reductase and polypeptide hydrolytase at 0.01 mol/L, whereas Na_ 2 S_ 2 O_ 3 , which acts on the disulfide bonds reductase, inhibits keratinase.On the condition that substrate, keratins exists, S.fradiae var S-221 is induced to produce exo-keratinase, which is a multiproteinase, containing disulfide bonds reductase, which is a key enzyme degrading keratins, then, with polypeptidic, hydrolytase, graduately hydrolyzates denatured keratins into polypeptides, oligopeptides and free amino acids, so that keratins have been decomposed completely.Sulfur in the keratins was transferred into sulfhydryl compounds, H_ 2 S and sulfates in the course of keratinolysine.
10.Single-direction video-assisted thoracoscopic surgery anatomic basal segmentectomy in 352 patients: A retrospective study in a single center
Chengwu LIU ; Qiang PU ; Jiandong MEI ; Yunke ZHU ; Lin MA ; Chenglin GUO ; Lunxu LIU
Chinese Journal of Clinical Thoracic and Cardiovascular Surgery 2022;29(10):1284-1289
Objective To share the clinical experience of video-assisted thoracoscopic surgery (VATS) anatomic basal segmentectomy by single-direction method. Methods The clinical data of 352 patients who underwent VATS anatomic basal segmentectomy in West China Hospital between April 2015 and April 2021 were retrospectively reviewed, including 96 males and 256 females with a median age of 50 (range, 26-81) years. All basal segmentectomies were performed under thoracoscopy, through the interlobar fissure or inferior pulmonary ligament approach, and following the strategy of single-direction and the method of "stem-branch". Results All patients underwent basal segmentectomy successfully (49 patients of uniportal procedure, 3 patients of biportal procedure and 300 patients of triportal procedure) without addition of incisions or conversion to thoracotomy and lobectomy. The median operation time was 118 (range, 45-340) min, median intraoperative blood loss was 20 (range, 5-500) mL, median drainage time was 2 (range, 1-22) d and median postoperative hospital stay was 4 (range, 2-24) d. The postoperative complications included pneumonia in 6 patients, prolonged drainage (air leakage duration>5 d or drainage duration>7 d) in 18 patients, cerebral infarction in 1 patient and other complications in 2 patients. All patients were treated well and discharged without main complaints. No perioperative death happened. Conclusion VATS anatomic basal segmentectomy is feasible and safe. It can be performed in a simple manner following the strategy of single-direction.