Objective To perform the performance verification of the Sysmex XT-2000i hematology analyzer.Methods Accord-ing to requirements of NCCLS EP5-A2,EP15-A2,EP6-A2,EP9-A2 documents,the accuracy,precision and linearity of the Symex XT-2000i hematology were evaluated and the detection results were compared with those detected by the Sysmex XE-2100i which participated in the external quality assessment(EQA)of Ministry of Public Health for long time.Results The accuracy,precision, linearity and contamination rate all are within the permissible range.Conclusion The performance of Sysmex XT-2000i is good,the determination results are reliable.As a perfect hematology analyzer,it can satisfy the hematological analysis of hospital clinical la-boratory.

Objective To study the influence of colon hydrotherapy on premature infants with jaundice.Methods Based on intravenously infusing yinzhihuang and albumin and enzyme inducer,the warmed normal saline was poured into colon by 10 mL/kg per 8 hours in 40 preterm infants,and compared with 44 cases of control group.Serum bilirubin of all patients were measured at 24,72,120,168,216 hours,respectively.The days of meconiumbeing eliminated completely were recorded.Results The bilirubinemia level in trial group were lower in trial group than that in control group at every time and the rate of hyperbilirubinemia of the test group was lower(?~2=4.073 P

Objective To explore the personality characteristics of abused children in order to reduce the incidence of child abuse.Methods Two hundred and ninty five middle school students were investigated with general questionnaire and Eysenck Personality Questionnaire of children. Eighty six students experiencing child abuse (CA) last year as study group and one hundred and ninety six non-abuse children as controls (NCA) were analyzed by means of Eysenck Personality Questionnaire of children.Results The score of neuroticism in CA group was significantly higher than that in the control group (55.62±10.60/52.65±10.98,t=-2.114 P=0.035). The score of lie in CA group was significantly lower than that in control group (42.21±9.87/46.04±9.20,t=3.184 P=0.002). On the impact of different sex, the psychoticism score of male was significantly higher than that in the control group(52.37±11.49/48.04±9.97,t=-2.227 P=0.028), and the lie score was significantly lower than that in control group(41.03±9.18/46.18±8.79,t=3.125 P=0.002).The scores of those in the female were not significant.Conclusions There is a close association between the unstable emotion and child abuse in children, so training emotional self-control and emotional expression of children might be a intervention strategy in the future. In addition, the frequency of lie in children is probably one of factors that determine whether children are abused or not.

AIM: To establish HPLC fingerprint of Achyranthes bidentata BI..METHODS: Ten batches of Achyranthes bidentata BI from different habitats were measured by RP-HPLC,and their fingerprints were obtained.C18 column was used.Acetonitrile and water gradient elution were adopted as a mobile phase,the flow rate was 1.0 mL/min,the detection wavelength was set at 250 nm,the injection volume was 20 ?L,and the column temperature was at 30 ?C.RESULTS: Fifteen common peaks were confirmed in fingerprints.CONCLUSION: This method is simple,credible and of good reproducibility,can be used for the quality control of Achyranthes bidentata BI..

This paper introduces the control system design of the medical oxygen generator in the field trailer.Based on a series of experiments,the results prove that the control system can meet the purpose of the design and can fulfill the need of the equipment.

Objective To study the relieving cough effect ofPuji-Xuanfei-Xiaodu decoction on acute bronchitis.Methods 87 patients with acute bronchitis of exterior cold and interior heat were randomly divided into two groups(44 cases in a therapy group and 43 cases in a control group).Puji-Xuanfei-Xiaodu decoction was given to the patients in the therapy group and acute bronchitis syrup was given to the control group, to observe the influence on symptoms of cough, sputum, fever and headache. The course of treatment was 7 days. Results On the reliving cough aspect, 28 cases (63.6%) showed cure effect and 13 cases (29.5%) effctive, the total effective rate was 93.2% in therapy group with 44 cases; while in control group (43 cases), 15 cases (43.9%) showed cure effect and 23 cases (53.5%) effctive, the total effective rate was 88.4%. The symptoms of cough, phlegm, fever, headache and aversion to wind were significantly decreased in two groups. In therapy group, the numbers of cure effect, remarkable effect and efficacy were 22 (50%) ,11(25%) and 8 (18.2%) cases respectively, the total effective rate was 93.19% while the numbers of cure effect, remarkable effect and efficacy were 14 (32.6%) ,8(18.6%) and 12 (27.9%) cases respectively, the total effective rate was 79.07% in control group. The total effective ratio of two groups had significant difference (P>0.05).ConclusionsPuji-Xuanfei-Xiaodu decoction is effective in relieving cough.

A rapid and sensitive method based on biotin-avidin mediated competitive enzyme-linked immu nosorbent assay(BA-ELISA) was established for the determination of ketamine.The optimal concentration of coated antigen and anti-ketamine monoclonal antibody were found to be 2.0 and 10.2 mg/L.The concentra tions of biotinylated goat anti-mouse IgG(Biotin-IgG) and streptavidin-horseradish peroxidase(SA-HRP) were optimized and the optimum results were found to be 0.29 and 1.0 mg/L, respectively.The linear range of the presented method was from 0.1 to 1000 μg/L, and the limit of detection was found to be 0.03 μg/L.The recoveries of ketamine spiked in human serum and urine were between 94% and 102%.Comparing the result of traditional ELISA, the present BA-ELISA method had a lower detection limit for ketamine.The experimental results indicated that the present BA-ELISA method was specific and sensitive.

ABSTRACT:Objective To verify whether rosuvastatin can inhibit homocysteine (Hcy)‐induced rat aortic vascular smooth muscle cell (VSMC ) phenotype transformation and its potential mechanism .Methods The primary culture and identification of rat VSMCs were conducted , using VSMCs in passage4‐7 for the following experiments .The VSMCs were divided into 4 groups:control group ,Hcy (100μmol/L) group ,Hcy+ rosuvastatin group ,and Hcy+ rapamycin group .MTT was used to investigate the proliferation of VSMCs .Transwell chambers and wound healing were employed to test the migration ability of VSMCs . ICC was used to detect the VSMCs'morphology .Western blotting was used to investigate the expressions of SM‐actin ,SM‐MHC ,calponin ,OPN ,and p‐P70S6K1 in VSMCs of each group .Results Compared with those in control group , the proliferation and migration ability of VSMCs were significantly increased in Hcy modulation group (P< 0 .01) .The expressions of SM‐MCH and calponin increased but those of OPN and p‐P70S6K1 decreased in Hcy group compared with control group (P<0 .01) .The expression of SM‐actin did not significantly differ between the groups .Compared with those in Hcy modulation group , the proliferation and migration ability of VSMCs were significantly decreased in rosuvastatin and rapamycin groups ( P<0 .01) .The expressions of SM‐MCH and calponin increased while OPN and p‐P70S6K1 expressions decreased in rosuvastatin and rapamycin groups compared with Hcy group ( P< 0 .01 ) . Conclusion Hcy can induce the dedifferentiation of VSMCs ,and rosuvastatin can inhibit this effect of Hcy .Its potential mechanism may be realized via the mTOR/P70S6K1 signal pathway .

Objective To observe the effect of Dezocine in preventing the adverse reactions during the operation of cesarean delivery.Methods We selected 60 pregnant women in the ASA level Ⅰ who were going to undergo the operation of cesarean delivery.Then we divided them into two groups in random:group A (studying group) and group B (control group),each group had 30 patients.After the fetus were delivered,the patients in group A were given intravenous injection of 1mg of dezocine,and the patients in group B were only given 1 ml saline.Then we observe the heart rate (HR),systolic blood pressure (SBP) and the degree of blood oxygen saturation (SpO2) in seven time points,and analyzed the incidence of contraction pains,chills,nausea and vomiting during the operation in both groups.The time points were when patients entered the operation room (T0),the level of anesthesia was established (T1),5 minutes after fetus were delivered (T2),10 minutes after fetus were delivered (T3),15 minutes after fetus were delivered (T4),30 minutes after fetus were delivered (T5) and the end of operation (T6).Results Compared with group A,the SBP at T2,T3 and T4 were obviously higher than those in group B (P＜0.05).And the HR increased at T2 to T6 in control group (P＜0.05) as compared with group A.The incidence of contraction pains were milder in group A than group B (P＜0.05).In group A,the incidence of chills was13.3％,and in group B,the rate was 30％.Conclusion Dezocine can prevent the adverse reactions during the operation of cesarean delivery.

Objective To explore the experience of nursing patients with Crohn’s disease.Method The nursing histories of 80 patients with Crohn’s disease were retrospectively reviewed to analyze and summarize the measures for nursing them.Result After active treatment and effective nursing,all the patients were improved and discharged.Conclusion Effective nursing is of positive significance to improve the curative effect of Crohn's disease.