Objective To investigate the impact of chemotherapy plus fluoxetine on depression and quality of life of tumor patients.Methods 160 cases cancer patients were randomly divided into the control group and the experimental group with 80 cases in each group.The control group received routine chemotherapy,while the experimental group adopted fluoxetine based on routine chemotherapy.The incidence of depression and improvement of life quality were compared between two groups.Results The incidence of depression was lower,and proportion of patients with good quality of life was higher in the experimental group compared with those of the control group.Conclusions To take chemotherapy plus fluoxetine has certain values in decrease depression and improve quality of life.

OBJECTIVE:To study the effect and its mechanism of dexmedetomidine(Dex)on inflammatory response in septic mice. METHODS:Mice were randomly divided into normal control group,model group,miR-146a inhibitor (50 mg/kg)+Dex (50 μg/kg)group,Dex low-dose,medium-dose,high-dose groups(10,30,50 μg/kg),10 in each group. Except for normal con-trol group,other groups were intraperitoneally injected lipopolysaccharide to induce septic models,intraperitoneally injected rele-vant medicines after 0.5 h. After drug intervention for 6 h,miR-146a expression,IRAK1 and TRAF6 mRNA expressions in periph-eral blood mononuclear cells in each group were detected by real-time fluorescence quantitative polymerase chain reaction method. IRAK1,TRAF6 protein expressions in peripheral blood mononuclear cells in each group were detected by Western blot method. TNF-α,IL-6 levels in serum were detected by enzyme-linked immunosorbent method. RESULTS:Compared with normal control group,miR-146a expression,TNF-α and IL-6 levels,IRAK1,TRAF6 mRNA and protein expressions in peripheral blood mononu-clear cells in model group were increased(P<0.01). Compared with model group,miR-146a expression in peripheral blood mono-nuclear cells in Dex medium-dose,high-dose groups were increased;TNF-α and IL-6 levels,IRAK1,TRAF6 protein expressions were decreased (P<0.05 or P<0.01);while IRAK1,TRAF6 mRNA expressions changed less obviously (P>0.05). Compared with Dex high-dose group,miR-146a expression,in peripheral blood mononuclear cells in miR-146a inhibitor+Dex group was de-creased;TNF-α and IL-6 levels,IRAK1,TRAF6 protein expressions were increased (P<0.05 or P<0.01);while IRAK1, TRAF6 mRNA expressions changed less obviously(P>0.05). CONCLUSIONS:Dex can inhibit the inflammatory response in sep-tic mice. The mechanism may associate with inducing miR-146a expression and inhibiting the 2 important adaptor proteins IRAK1, TRAF6 expressions in Toll-like receptor 4/nuclear factor-κB pathway.

Community Health Service ( CHS) not only contains the treatment, but also considers the care as its responsibility. Therefore, its character of humanity service can be showed in the practice. However, besides the humanity, CHS needs some reasonable forms such as laws, systems as well, to protect its development and community habitants health. The author thinks that when the humanity and reasonableness conjunct on the solution and prevention for the medical tangle which occurs in the CHS, maybe the medical Ttreatment will come back to its birthday on which it ensured "care and console" as its duty, and it also can decrease the medical danger at length, then serve for the society and benefit for the people.

0.05).While the diagnosis ratio of MP and LP with RT-PCR+IIF method were higher than any of other 3 methods significantly(Pa

Objective To investigate the alleviative effects of lidocaine postconditioning on pulmonary injury following ischemia reperfusion. Methods Seventy-two adult SD rats were randomized to 4 groups; sham group, ischemia-reperfusion (I-R) group, ischemic postconditioning(IPC) group and lidocaine postconditioning group. The pulmonary ischemia-reperfusion model was established by occlusion of the left hilum of lung for 45 min and the reperfusion was taken by removing the clamp for 2 h. At the moment of reperfusion, lidocaine 4 mg/kg was injected as a priming dose following a continuous rate of 4 mg/(kg · h). PaO_2, TNF-α, W/D of left lung, the level of MDA of left lung tissue were measured. At the end of reperfusion left lung was removed for microscopy. Results After reperfusion PaO_2 of lidocaine group was much higher than that of I-R group (P<0.05). Lidocaine postconditioning induced a significant decrease in the level of MDA of lung tissue[(7. 03±1.17) μmol/L] compared with ischemia reperfusion group [(8.77±1.42) μmol/L] (P<0.05). Lidocaine postconditioning resulted in a lower level of TNF-α [(1. 69±0.34) μg/L] than that of I-R group [(2. 52±0. 54) μg/L] (P < 0. 05). Microscopic examination showed that lidocaine postconditioning could decrease the level of edema of left lung and accumulation of neutro-phils. Conclusion Lidocaine postconditioning exerts a protective effect on pulmonary ischemia-reperfusion injury administered in the beginning of reperfusion. The effect may be explained by to the antioxidant effect and the suppression of expression of TNF-α.

Purpose:To compare with the efficacy in the immediate effects and toxicities on patients with advanced gastric cancer treated with 5-fluorouracil (5-FU) and leucovorin (LV) combined with oxaliplatin or paclitaxel. Methods:Forty patients with advanced gastric cancer, whose metastases to organs or sites included liver, lymphy node, abdominal cavity, abdominal wall, etc, were enrolled in this study, and was randomly divided into two groups (A and B groups). The A group of 20 patients (70% of them were retreated patients) were treated with a combination of oxaliplatin, leucovorin(LV) and 5-fluorouracil(5-FU) continuous infusion regimen. The B group of 20 patients (55% of them were retreated patients) were treated with a combination of paclitaxel, leucovorin (LV) and 5-fluorouracil(5-FU) continuous infusion regimen. Results:Of the 40 evaluable patients, there were two complete responses and seven partial responses (response rate 45%) in the A group, and nine partial responses (response rate 45%) in the B group. All patients were evaluable for toxicities. The most common toxicities were bone marrow depression,peripheral neuropathy,digestive tract toxicities and liver function damage in the A group. The most common toxicities were bone marrow depression and liver function damage in the B group. Conclusions:These two regimens (5-fluorouracil and lcucovorin combined with oxaliplatin or paclitaxel) showed good efficacy and acceptable toxicities in advanced gastric cancer patients, and the 5-fluorouracil and leucovorin combined with oxaliplatin regimen may have some virtues, such as economics, convenience of medication and less serious toxicities.[

Homogenous fat extract was injected through the femoral vein to induce respiratory distress syndrome in 15 dogs. It was found that the changes of blood gases, chest x-ray films, and lung pathology of the dogs were similar to those of adult respiratory distress syndrome.The pathogenesis was extensive pulmonary fat embolism with complement activation and free radicals formation. Vitamin E was consumed during antiperoxidation. It is believed that this model serves better for the study of respiratory distress syndrome.

The therapeutic effects of prostaglandin E1 on respiratory distress syndrome induced with homogeneous fat extraction were observed in dogs.It was found that prostaglandin E1 could alleviate hypoxemia,reduce pulmonary capillary permeability,and attenuste pulmonary edema.The mechanism of the therapeutic efficiency of prostaglandin E1 on pulmonary damages is that prostaglandin E1 can inhibit the adherence of polymorphonuclear netttrophils and the genesis of oxygen free radicals,and protect the pneumocyte type Ⅱ.

Objective To evaluate the clinical and stenting relating risk factors on the in-stent restenosis. Methods One hundred and thirty seven patients whose data were available were enrolled in this study, and the patients were divided into the groups of in-stent restenosis and non in-stent restenosis according to the results of coronary angiograms. Then the clinical and stent relating factors affecting in-stent restenosis were analyzed. Results There were no statistical differences in age and prevalence of smoking, drinking, hypertension and diabetes mellitus between two groups. Narrowing of target coronary vessels before the procedures in the group of in-stent restenosis was more severe than that in the group of non in-stent restenosis ( vs , P=0.033). The average diameter of stents used in the group of in-stent restenosis was obviously smaller than that used in the group of non in-stent restenosis ([3.19?3.90]mm vs mm, P=0.005), the average length of stents, however, used in the group of in-stent restenosis was apparently longer than that used in the group of non in-stent restenosis ([21.91?8.98]mm vs [18.20?6.07]mm, P=0.011). The total duration of stent inflation in the group of in-stent restenosis was obviously shorter than that in the group of non in-stent restenosis ([22.74?19.56]s vs [29.12?25.72]s, P=0.026). There was no statistial difference between the two groups in the inflation times and pressure of stents implantation. Conclusion The results suggested that the rate of in-stent restenosis was not significantly influenced with age, smoking, drinking, hypertension, diabetes mellitus and inflation times and pressure of stent implantation, but the narrowing of target vessels before procedures and the lengths of the stents were positively related to in-stent restenosis, while stent diameters and total inflation durations of stent were negatively related to in-stent restenosis.

BACKGROUND: Excessive nitric oxide (NO) release can cause the occurrence and development of brain injury and senile dementia due to the apoptosis induction role of NO at high concentration to nerve cells. Therefore one strategy to prevent and treat senile dementia is inhibiting the apoptosis induced by NO.OBJECTIVE: To observe whether acidic peptide will inhibit the neuron apoptosis caused by NO. DESIGN: An cell and molecule observation experiment by comparisons. SETTING: Department of Biochemistry and Molecular Biology of Basic Medical College in Zhengzhou University and the Second Laboratory of Biological Active Peptide Institute in Zhengzhou University. MATERTALS: The experiment was performed between May 2003 and May 2005, in the Second Laboratory of Biological Active Peptide Institute in Zhengzhou University and the cell culture room of Department of Biochemistry and Molecular Biology of Basic Medical College in Zhengzhou University. The newborn SD male rats within 24 hours after birth were provided by the Animal Center of Henan Province (410117).METHODS: On day 11 of primary cultures, hippocampus neurons of the newborn SD rats were pretreated with different dosages of acidic peptide for six hours. Sodium nitroprusside (SNP) of 50 μmol/L final concentration was added to the cells which were incubated for another 24 hours. Cells were collected and adopted in this experiment of five different groups, namely normal control group, group treated with SNP, group of SNP plus 0.037 5 mg/mL acidic peptide, group of SNP plus 0.075 mg/mL acidic peptide, group of SNP plus 0.15 mg/mL acidic peptide. The cell's survival rate wasmeasure by methyl thiazolyl (MTT) method; The neurofilament protein was stained with the method of immunohisto chemistry. The shape of apoptosis was display with acridine orange fluorescent stain. Then DNA ladder zone of apoptosis cells was analyzed with the method of agarose gel electrophoresis. Western Blot and absorbance scan were used to determine the expression level of Bcl-2 protein and Bax protein.MAIN OUTCOME MEASURES: ①Experimental result of cell survival rate with MTT method;②Observation results of nuclear type of apoptosis; ③DNA electrophoresis analysis of apoptosis; ④Western Blot analysis results of Bcl-2 protein and Bax protein.RESULTS: ①Neuron survival rate was 58.9％ for group treated with SNP, 70.0％ for group of SNP plus 0.037 mg/mL acidic peptide, 72.8％ for group of SNP plus 0.075 mg/mL acidic peptide, and 75.3％ for group of SNP plus 0.15 mg/mL acidic peptide. ②Observation results of nuclear type of apoptosis: Significant characteristics of apoptosis were seen in group treated with SNP. The nucleus of hippocampus neuron treated with different concentrations of acidic peptide plus SNP was similar to that of normal control group in morphology. ③The results of DNA electrophoresis analysis of apoptosis: Only the neuron DNA of group treated with SNP showed clear characteristic DNA ladder zone of apoptosis on agarose gel electrophoresis. ④Analysis results of Bcl-2 protein and Bax protein with Western Blot and absorbance scan: The expression level of Bcl-2 protein in SNP treated group was decreased while that of Bcl-2 protein was increased. Bcl2 protein levels in acidic peptide plus SNP group were increased and Bax protein levels were decreased gradually with the increasing concentrations of acidic peptide compared with SNP treated group. CONCLUSION: Acidic peptide can inhibit neuron apoptosis, increase expression level of neuron Bcl-2 protein and decrease expression level of neuron Bax protein.