Objective:To determine the three-dimensional stability of atlantoaxial reconstruction with anterior approach screw fixation through C2 vertebral body to C1 lateral mass and Gallie’s technique for C1~2 instability.Methods:25 human cadaveric specimens were randomly divided into 5 groups.The three-dimensional range of motion C1 relatively C2 was measured under five different conditions,the intact state(intact group),odontoid tape Ⅱ fracture(group OTF),fixation with posterior C1~2 transar- ticular screw(group PTS),anterior approach screw fixation through C2 vertebral body to C1 lateral mass(group ASM),ASM and Gallie’s technique(group ASMG). The obtained data were statistically analyzed. Results:The range of motion in all directions group ASMG,ASM and PTS was significantly decreased.There was significant difference among group ASMG,ASM and PTS in flexion/extension and lateral bendings by statistical analysis(P0.05). Conclusion:In vivo biomechanics studies showed that ASMG had unique superiority in the reconstruction of the atlantoaxial stability,especially in controlling the instability of flexion/extension and lateral bendings.It was one kind of reli- able surgical choice for the treatment of the obsolete insta- bility or dislocation of C1~2 joint.

Angioplasty, Balloon, Coronary ; Humans

Animals ; China ; Culex ; Insect Vectors ; Longitudinal Studies

The paper introduces microcosmic control model for medical morals construction.The function of the model includes explanation,operation and evaluation.The author holds that the key to the morals construction is scientific management,and the decision maker should have the social responsibility and the love-patient consciousness.

AIM: To study the effects of arsenic trioxide (As_2O_3) on mouse airway fibroblast (FB) proliferation and expression of c-myc and c-sis in cultured FB. METHODS: The cultured FB was divided into seven groups. NS, dexamethasone (1.0 ?mol/L) and As_2O_3 at concentrations of 0.25, 0.5, 1.0, 2.0, 4.0 ?mol/L were instilled respectively into cultured FB. The effects of different concentration of As_2O_3 and dexamethasone on cell growth were observed at 1, 3, 5 and 7 day. The changes of cell cycle and the positive expression rate of c-myc and c-sis were examined by flow cytometry (FCM). RESULTS: Various concentrations of As_2O_3 significantly inhibited the proliferation of FB in vitro and showed a dose-dependent and time-dependent tendency. Data from FCM indicated that G_1-phase cell percentage increased and G_2/M-phase cell percentage decreased with the increase in As_2O_3 concentrations. The expression of c-myc and c-sis was significantly inhibited by As_2O_3 (2.0, 4.0 ?mol/L). CONCLUSION: As_2O_3 inhibits FB proliferation by downregulating the expression of c-myc and c-sis.

Objective To investigate the therapeutic effect of transplantation of limbal epithelial cells and decellularized porcine cornea construct in rabbits with corneal alkali burn.Methods Allogeneic rabbit limbal epithelial cells were cultured and identified.Porcine cornea scaffold was fabricated by decellularization.The LECs-DPC construct was cultured and transplanted to the alkali-burned corneal surface of rabbits(4month follow-up).Results Immunocytochemistry and RT-PCR analyses revealed the presence of ABCG2 positive limbal stem cells.A general view showed the corneas were clear and vessel-free postoperatively.HE and Masson Trichrome staining results indicated the epithelial stratification,fewer cellular infiltration,DPC' integration into host corneal tissues,and more regular collagen configuration.Transmission electron microscopy exhibited formation of desmosomes and microvilli.Conclusion Allogeneic limbal epithelial cells transplantation using DPC as a carrier can restore corneal clarity after alkali burn in a rabbit model.

Objective To establish the content determination method of berberine hydrochloride in Sifangwei capsules.Methods HPLC method was used with octadecylsilane chemically bonded silica as the filler,acetonitrile-0.05 mol/L KH2PO4 solution(25∶75) as the mobile phase,detective wavelength was 230 nm.The number of theoretical plates should not be less than 3 000.Results The content determination method has a good linear relation at the range of 0.201 6～1.008 ?g(r=0.999 8).The recovery rate was 98.7%,RSD=1.24%(n=5).Conclusion The method is simple,accurate,with good separation and reproducibility,and can be used for the quality control of Sifangwei capsules.

In this paper, we describe mismatch, chaos, and disorder behavior during healthcare seeking. Such structural imbalances, scarcity and surplus make healthcare services less efficient. We give reasons for such phenomena in terms of long-term effects of health resource allocation policy-making and implementation, inherent de-fects within healthcare insurance, incomplete market development, and cultural issues. We then propose four policy strategies, including rational price mechanism, strict referral healthcare system, confidence in basic healthcare facili-ties, and proper healthcare cultures. These will restore orderly healthcare behavior and help realize expectations for the current healthcare reform in China.

Objective To seek a operation method of quality control of biochemical tests in peacekeeping mission via perspective of evidence-based laboratory medicine .Methods Literatures related to quality control of biochemical tests in international peace-keeping were referred ,compared and discriminated .Combined with 5-year peacekeeping laboratory experience ,a set of feasible ap-proaches were worked out and a practice evaluation was performed in a task period .Results Under the supervision of the quality control system ,providing accurate and reliable test results to the clinical ,developing and implementing quality control of biochemical tests were economical ,practical and reliable ,and could guarantee the effectiveness of reports of biochemical test in peacekeeping medical institutions .Conclusion Evidence-based laboratory medicine is important for quality control of biochemical tests in peace-keeping medical institutions .

BACKGROUND:Internal fixation has been shown to decrease intertrochanteric fracture complications in the clinic and to improve quality of life of patients. The major method widely used in the clinic is the fixation of proximal femoral nail and dynamic hip screw. ＜br> OBJECTIVE:To observe the clinical effects of proximal femoral nail and dynamic hip screw in treatment of intertrochanteric fracture in the elderly. ＜br> METHODS:A total of 60 patients with intertrochanteric fracture were randomly divided into proximal femoral nail and dynamic hip screw groups (n=30), and they respectively received proximal femoral nail and dynamic hip screw fixation. ＜br> RESULTS AND CONCLUSION:Compared with the dynamic hip screw group, operative time, blood loss, the incidence of complications one year after repair, fracture healing time and complications were lower in the proximal femoral nail group (P＜0.05). After repair, the excellent and good rate of Harris hip scores was higher (P＜0.05). No significant difference was detected in healing time of wound between the two groups (P>0.05). Results verified that compared with the dynamic hip screw fixation, the clinical effects of proximal femoral nail fixation were better, more safe and reliable in the treatment of intertrochanteric fracture in the elderly.