Natamycin is a polyene macrolide antifungal antibiotic ,which is wide ly used in the food industry in order to prevent mould contamination .Biosynthe s is gene cluster of natamycin is discovered by the overall of progress in molecul ar biology of natamycin, including 16 open reading frames which includes the gen e for 26-member ring formation of natamycin (pimS0-pimS4 ) and the modifying gene s, and the function of the protein including polyketide synthases(PKSs)、PimD、P imJ、PimK were studied

OBJECTIVETo analyze the relationship between inorganic ingredients (Ca, P and F) concentration of plaque residues in the primary teeth and caries status, sugar exposure frequency.

METHODSSubjects were 93 of 3-5 years old children living in Beijing area. According to their caries status, they were classified into two groups: Severe early childhood caries(S-ECC) group and caries free(CF) group. Plaque samples were collected from subjects respectively. Ca, P and F concentration of plaque residues was measured by atomic absorption spectroscopy, spectrophotometer and fluoride electrode respectively. The information of daily sugar exposure frequency of children was obtained from a standardized questionnaire filled by their parents. The relationship between inorganic ingredients (Ca, P and F) concentration of plaque residues and daily sugar exposure frequency was analyzed and the relationship between inorganic ingredients (Ca, P and F) concentration of plaque residues and caries status in the S-ECC group was also analyzed.

RESULTSThe mean values for Ca, P and F concentration of plaque residues were lower in the S-ECC group, but the difference was not statistically significant (P > 0.05). The daily sugar exposure frequency in the S-ECC group was higher than that in the CF group. There were no relationships between Ca, P and F concentration of plaque residues and caries status (dmft, dmfs), sugar exposure frequency in the primary dentition.

CONCLUSIONPlaque residues from CF subjects did not contain a greater pool of calcium, inorganic phosphorus, and fluoride ions than that from S-ECC subjects.

Calcium ; Carbohydrates ; Child ; Child, Preschool ; Dental Caries ; Dental Caries Susceptibility ; Dental Plaque ; Fluorides ; Humans ; Phosphates ; Tooth, Deciduous

Strain QJ16,one of some methanotrophic bacteria isolated from soil samples utilizing the separate device designed by our laboratory;was studied in detail and identified as one of the Genus methylomonas through 16S rDNA sequencing and comparison. The culturing and reaction conditions for methane utilization of this strain were investigated. The results indicated that the optimal conditions for the growth of strain QJ16 were methane gas as carbon source and the coexistence of NH4Cl and KNO3 as nitrogen source,the cultivation temperature of 30℃ and medium initial pH 6～7,and the optimal Cu2+ concentration of 15 ?mol/L.

Objective To establish the optimum preparation process for Syrigopicroside Dropping Pills.Methods The preparation conditions,such as the proportion between the extracts and matrix,dripping temperature,the ratio of matrix,were studied with orthogonal test.The obtained results were tested by three indexes,such as the variation coefficient of weight of pill,time limit of dissolution and appearance of the dropping pills with analysis of variance.The preparation process was optimized.Results The optimal technical conditions were as follows:the ratio of PEG4000 and PEG6000 as matrix was 4∶1,the ratio of syrigopicroside to matrix was 1∶4,and the dripping temperature was 80 ℃.Conclusion The preparation technology can meet the requirement for dripping pill specified in China Pharmacopeia(2005 Edition).

Objective Study on the traceability of serum enzyme ass ays by testing enzyme reference material in clinical laboratories. Methods 50 laboratories were involved in this survey. One enzyme reference material was send to each participate lab. The reference material was tested by use of routine method and the results were recorded. All lab data were processed with computer. Results Compare with the target values, the bias of alanine aminotransferase (ALT), aspartate aminotransferase (AST), creatine kinase (CK) and lactate dehydrogenase (LD) assays were 6 3%, 5 5%, -5 9% and -5 0% respectively, the bias of alkaline phosphatase (ALP) assays was -36 6. The inter laboratory coefficient variation of the amylase assay was 29 2%. Conclusions The results of enzyme assay in clinical laboratories could be traced to the international reference material.

Objective:To explore the relationship between marital satisfaction and character strengths in young people. Methods:An online survey was delivered to 307 married people (aged 2 1 -44 with years of mar-riage 0-20). Marital satisfaction was measured with the Evaluating and Nurturing Relationship Issues,Communica-tion,and Happiness (ENRICH,including intrapersonal,interpersonal,and external factors and marital satisfaction). Character strengths were measured with Values in Action Inventory of Strengths (VIA-IS,total 24 character strengths were classified into 4 high level character strength factors including heart-focus on self,heart-focus on oth-ers,mind-focus on self,mind-focus on others). Results:All 24 character strengths scores and 4 high level character strength factors scores were positively correlated with marital satisfaction score (r=0. 17 -0. 53,Ps<0. 01 ). Four high level character strength factors positively affected marital satisfaction through the mediation of 3 marital factors. Conclusion:The results indicate that the young people's marital satisfaction is closely related to their char-acter strengths,and character strengths affect marital satisfaction through 3 marital factors.

Acetylcholine ; pharmacology ; Cell Line, Tumor ; Cell Membrane ; Epithelial Cells ; cytology ; Gastric Mucosa ; cytology ; drug effects ; metabolism ; Humans ; Receptors, Nicotinic ; drug effects ; metabolism ; Stomach Neoplasms ; metabolism

Congresses as Topic ; Humans ; Otorhinolaryngologic Surgical Procedures