1.Acetabular medial wall displacement osteotomy in total hip arthroplasty for treatment of adult hip hysplasia An 8-case analysis
Huimin GUO ; Qi LIAO ; Huabin HUANG
Chinese Journal of Tissue Engineering Research 2009;13(17):3392-3396
The present study retrospectively analyzed 8 patients with hip dysplasia (8 hips) comprising 2 males and 6 females, aged 46 years old (range, 35-65 years old) who received treatment at the Department of Orthopedics, Second Affiliated Hospital of Nanchang University between September 2004 and November 2006. Preoperative radiographs and CT examination showed that all patients presented with sharp angle of affected hip > 45°, poor containment of femoral head, narrow joint space, and non-successive Shenton's line. Following Crowe classification, there were 5 cases (5 hips) of grade Ⅱ,and 3 cases (3 hips) of grade Ⅲ. Preoperative Harris hip score was 50 points (range, 44-62 points). Total hip arthroplasty was employed in each patient. Prosthesis was fixed by Cementless or cemented modes. Cementless fixation was applied in 6 hips and cemented fixation in 2 hips. Cementless prosthesis was used on the affected side in each patient. Replacement time, bleeding volume during surgery, hospital days post-surgery, Harris scores, and peri-operative complications were recorded.Simultaneously, post-surgery healing was observed. All 8 cases were followed up for an average period of 6 months. All patients exhibited well recovered joint function. No infection, sciatic nerve injury, dislocation of hip joint, fracture of femoral shaft, thrombosis of deep veins, or prosthesis loosening were found in any follow-up case. Six months after surgery, Harris hip score was 88 points (range, 86-94 points). Radiographs taken at 3 months post-surgery displayed the bone union of surgical region. All these findings suggest that acetabular medial wall displacement osteotomy in total hip arthroplasty produces obvious early- and middle-stage effects in treatment of hip dysplasia.
2.Heart-lung transplantation for 4 patients with Eisenmenger’s syndrome
Huimin FAN ; Zhongmin LIU ; Jianhua GUO
Chinese Journal of Organ Transplantation 2003;0(05):-
Objective To summarize the experience of heart-lung transplantation.Methods Four patients with Eisenmenger’s syndrome underwent heart-lung homoplastic transplantation. All patients were complicated with severe pulmonary hypertension in New York Heart Association ( NYHA ) functional class IV. Cannulation for cardiopulmonary bypass consisted of a cannula in the high ascending aorta and separate vena caval cannulas. The heart-lung graft was moved into the chest, beginning with passage of the lung before the phrenic nerve pedicle. The bronchus was trimmed, leaving two cartilaginous rings proximal to the orifice of the upper lobe. The tracheal anastomosis was performed with a continuous 4-0 polypropylene suture, with the posterior portion continuously and anterior interrupted. The lungs were then ventilated (
3.Selection of peptides bind to PreS1 antigen from a phage-displayed peptide library
Qingqing CHEN ; Huimin ZHENG ; Baoyu GUO
Academic Journal of Second Military Medical University 2000;0(07):-
Objective:To screen for peptides that specifically bind to PreS1 antigen from a phage-displayed peptide library.Methods: The PreS1 antigen was used as the target molecule to screen the binding peptide from the Ph.D.-7 peptide library with phage display technique,and the positive clones were identified by ELISA.Results: After three rounds of biopanning,the binding peptides were screened from the peptide library by ELISA and competitive inhibiting ELISA.Sequencing result showed that the binding peptides had high affinity and specificity.Conclusion: A peptide binding PreS1 antigen has been successfully obtained by screening the phage display library,which paves a way for the diagnosis and treatment of hepatitis B infection.
4.Study on Adsorption-Reaction Formaldehyde Remover
Guangmei GUO ; Shufang LI ; Huimin DUAN
Journal of Environment and Health 1993;0(01):-
Objective To develop a remover of formaldehyde in indoor air. Methods Activated carbon was used as the raw materials, potassium hydroxide as the activator. The activated carbon was modified through activation and impregnation. The influence factors for the formaldehyde adsorption property of activated carbons were evaluated, such as the size, activation temperature, activation time and impregnation time. Results The optimum technics conditions for developing the formaldehyde remover and the optimum conditions to absorb formaldehyde were confirmed. The mass adsorption capacity of the adsorbent was 49.6%, the BET surface area was about 1 591 m2/g and the total pore volume was 1.01 cm3/g. Conclusion The mass adsorption capacity of formaldehyde and the BET surface area of the modified activated carbon in the present paper are better than those of unmodified activated carbon.
5.Acetabular medial wall displacement osteotomy in total hip arthroplasty for treatment of adulf hip dysplasia An 8-case analysis
Huimin GUO ; Qi LIAO ; Huabin HUANG
Chinese Journal of Tissue Engineering Research 2007;0(17):-
45?,poor containment of femoral head,narrow joint space,and non-successive Shenton's line.Following Crowe classification,there were 5 cases(5 hips) of grade II,and 3 cases(3 hips) of grade III.Preoperative Harris hip score was 50 points(range,44-62 points).Total hip arthroplasty was employed in each patient.Prosthesis was fixed by Cementless or cemented modes.Cementless fixation was applied in 6 hips and cemented fixation in 2 hips.Cementless prosthesis was used on the affected side in each patient.Replacement time,bleeding volume during surgery,hospital days post-surgery,Harris scores,and peri-operative complications were recorded.Simultaneously,post-surgery healing was observed.All 8 cases were followed up for an average period of 6 months.All patients exhibited well recovered joint function.No infection,sciatic nerve injury,dislocation of hip joint,fracture of femoral shaft,thrombosis of deep veins,or prosthesis loosening were found in any follow-up case.Six months after surgery,Harris hip score was 88 points(range,86-94 points).Radiographs taken at 3 months post-surgery displayed the bone union of surgical region.All these findings suggest that acetabular medial wall displacement osteotomy in total hip arthroplasty produces obvious early-and middle-stage effects in treatment of hip dysplasia.
6.Effects of Butylphthalide on Neurological Function and Prognosis of Elderly Patients with Hypertensive In-tracerebral Hemorrhage after Trepanation and Drainage Surgery
Yaohui JIA ; Huimin CHEN ; Xianli GUO ; Yongkai GAO ; Yongqing DAI
China Pharmacy 2017;28(26):3701-3704
OBJECTIVE:To investigate the effects of Butylphthalide and sodium chloride injection on neurological function and prognosis of elderly patients with hypertensive intracerebral hemorrhage(HICH)after trepanation and drainage surgery. METH-ODS:During Jan. 2015 to Jun. 2016,80 elderly HICH patients were selected from our hospital and then divided into control group and observation group according to random number table,with 40 cases in each group. Both group received trepanation and drain-age surgery. Control group was given routine treatment. Observation group was given Butylphthalide and sodium chloride injection 100 mL,ivgtt,bid,on the fifth day after surgery,on the basis of control group. Both groups received treatment for 14 d. Clinical efficacies of 2 groups were observed. CSS scores were compared between 2 groups before surgery and 28 d after operation;volume of encephaledema,serum levels of homocysteine(HCY)and substance P(SP)were compared between 2 groups before surgery and 14 d after operation. RESULTS:The total response rate of observation group was 87.5%,which was significantly higher than 67.5% of control group, with statistical significance (P<0.05). Before surgery, there was no statistical significance in CSS scores,volume of encephaledema or serum levels of HCY and SP between 2 groups(P>0.05). CSS scores 28 d after operation, SP levels 14 d after operation were significanthy increased,volume of encephaledema and serum levels of HCY in 2 groups were significantly decreased,and the observation group was significantly better than the control group,with statistical significance(P<0.05). CONCLUSIONS:Butylphthalide and sodium chloride injection can significantly improve clinical efficacy and hepatic func-tion damage,relieve postoperative encephaledema,reduce serum levels of HCY and increase SP levels in elderly HICH patients af-ter trepanation and drainage surgery.
7.Study on the changes and clinical significance of the DNT cells and T lymphocyte subtype in children with infectious mononucleosis
Huiqin XUE ; Lei ZHU ; Rui ZHAO ; Huimin GUO ; Weili DU
Journal of Chinese Physician 2010;12(6):772-774
Objective To study on the changes of the DNT cells and T lymphocyte subtype in children with infectious mononucleosis (IM) and its clinical significance.Methods The flow cytometry (FCM) was used to detected the DNT cells and other T lymphocyte subtype in 48 cases of IM.Results The study showed that DNT cells( 9.39 ± 4.89 )% were greatly increased in comparison with normal controls (NC) (4.26 ± 1.68)% ( P <0.01 ).CD4 cells(21.45 ±9.87)% were decreased ( P <0.01 ) and CD8 cells increased in comparison with NC (32.43 ± 5.07) % ( P < 0.01 ).Conclusion DNT cells and T lymphocyte subtype can be used to evaluate the immune function of children with infectious mononucleosis (IM) and provide guidance for adoptive immunotherapy.
8.Association of (CCTTT) n microsatellite polymorphism of iNOS gene with diabetic nephropathy in Chinese Hans
Zheng ZHANG ; Huimin PENG ; Suhua ZHANG ; Fengjin GUO ; Ning YAN
Chinese Journal of Endocrinology and Metabolism 2000;0(06):-
The frequency of inducible nitric oxide synthase (iNOS) gene (CCTTT) 14 allele was significantly lower in diabetic nephropathy group (0.044) than that in diabetics without nephropathy (0.170, P
9.Mutation analysis of the OSMR gene in two Chinese families with familial primary cutaneous amyloidosis
Duyi GUO ; Tianji KANG ; Huimin YAN ; Huijuan ZHAO ; Wei JIANG
Chinese Journal of Dermatology 2017;50(2):91-94
Objective To detect mutations in the OSMR gene in 2 Chinese families with familial primary cutaneous amyloidosis (FPCA),and to analyze their relationship with clinical manifestations.Methods Clinical data were collected from 2 families with FPCA,and genomic DNA was extracted from peripheral blood samples.PCR was performed to amplify 18 exons and their flanking sequences of the OSMR gene followed by DNA sequencing in 2 probands and their family members.One hundred healthy individuals served as controls.Results In the first family,a heterozygous mutation (c.2081C > T) in exon 15 of the OSMR gene,which leads to a codon change at amino acid position 694 (p.P694L),was identified in the proband,as well as in the other 4 patients.In the second family,a heterozygous mutation (c.1538G >A) in exon 11 of the OSMR gene,which causes a codon change at amino acid position 513 (p.G513D),was identified in the other proband and her mother,suggesting the cosegregation of the gene mutation with the disease.None of the above mutations were detected in the healthy family members or controls.Conclusion The heterozygous mutations p.P694L and p.G513D in the OSMR gene may be associated with primary cutaneous amyloidosis.
10.High-throughput sequencing analysis of gut microbiome in patients with severe acne vulgaris and healthy individuals
Huimin YAN ; Huijuan ZHAO ; Duyi GUO ; Chunlei ZHANG ; Wei JIANG
Chinese Journal of Dermatology 2017;50(3):172-176
Objective To compare differences of gut microbiome between patients with severe acne vulgaris and healthy individuals by using high-throughput sequencing technology.Methods Stool samples were collected from 10 outpatients with severe acne vulgaris and 10 age-and sex-matched healthy controls.Then,the bacterial DNA was extracted and subjected to 16S rRNA sequencing for the identification of microbial species,and the differences of gut microbiome were compared between the patients and controls.Results There were no significant differences in the diversity of intestinal microflora,but only the relative abundance of a few bacteria differed significantly between the two groups.Gut microbiome in the two groups mainly consisted of Bacteroidetes,Firmicutes,Proteobacteria and Actinobacteria.There were no significant differences in the relative abundance of bacteria at the phylum and genus levels between the two groups.However,the relative abundance of Blautia producta and Coprococcus eutactus at the species level differed remarkably between the two groups.Conclusion No significant differences in the bacterial diversity indices are found,but some bacterial species significantly differ between the patients with severe acne vulgaris and healthy controls.