[Objective]To prove the feasibility of using the distance between sagittal plane of the spinal process and the enter point to individualize the placing of pedicle screw.[Method]Thirty spine specimen were collected and divided into two groups,data were measured,such as the width of the pedicle,distance between the enter point and anterior border of the vertebra,distance between sagittal plane of the spinal process and the enter point,angle from the longitudinal axis of the pedicle to sagittal axis of the vertebra,angle from the longitudinal axis of the pedicle to vertical line of the operating table.In group one the pedicle screws were placed with the help of the distance between sagittal plane of the spinal process and the enter point,the other by the method advised by Ebraheim.CT scan was applied to evaluate the place of the screws,according to the perforation extent,they were classified into 4 grades:A=totally in the pedicle;B=perforation extent4mm.[Result]The individualized group showed much lower perforation rate than the traditional method group in T3~10,and similar in T1,T2,T11,12.[Conclusion]It can obviously improve the accuracy of the pedicle screw placement to use the distance between sagittal plane of the spinal process and the enter point to localize the enter point,especially when anatomic landmark such as articulationes zygapophysiales and transverse process change.

Objective To observe the therapeutic effects of treating shingles with venesection and cuppingplus Yunnan White Powder external application and to observe the effect of mental intervention and nursing on clinical healing time.Methods 100 cases with shingles were randomly recruited into two treatment groups(Group A and Group B)and a control group.Both treatment groups were treated with venesection and cupping plus yunnan white powder external application,in addition,group B was treated with mental intervention and nursing care.The control group was treated with Aciclovir ointment.Results As compared with the control group,both treatment groups showed significant difierences in the time of relieving pain,removing swell,forming a scab,and healing(P＜0.01);Compared with group A,group B showed a shortened healing time with P＜0.05.Conclusion It is effective to treat shingles with venesection and cupping plus yunnan white powder.Mental Intervention and nursing can promote the healing time and improve the degree of satisfaction.

Adenoma ; metabolism ; Adrenal Cortex Neoplasms ; metabolism ; Biomarkers, Tumor ; metabolism ; Calcium-Binding Proteins ; metabolism ; Carcinoma, Neuroendocrine ; metabolism ; Humans ; Lung Neoplasms ; metabolism ; Neuroendocrine Cells ; metabolism ; Neuroendocrine Tumors ; metabolism ; Secretagogins ; Small Cell Lung Carcinoma ; metabolism

Objective To intelligently adjust the driving force of a remote-controlled drug delivery capsule based on the postural information in intestines,so as to achieve the control of speed and dosage of drug delivery and reduce the energy consuming.Methods A six-axis sensor MPU6050 was used,and its Z axis was coincident with the central axis of the drug delivery capsule so as to made the medicine discharge port pointing to the Z direction.The angular velocities and accelerations of X,Y,Z axes were monitored,transformed to quaternion with built-in digital motion processor,and then converted to Euler angle form.The intelligence adjustment of driving force could be achieved by accessing the real-time attitude angle of the capsule.Results The results of experiments showed that the error between the actual and the attitude angle measured by the sensor was less 1°.Conclusions The intelligent adjustment scheme of driving force of a gastrointestinal remote-controlled drug delivery capsule was designed.The real-time attitude angles of the capsule could be obtained with high spped and precision.This study can provide the theoretical and experimental basis for the intelligent adjustment of the driving force.

Objective To explore the in vitro isolation of ?2m-/Thy-1+ bone marrow-derived liver stem cells(BDLSCs) which bear double features of stem and liver cells from bone marrow stem cells(BMSCs)as so to provide suitable donor cells for the treatment of liver diseases by cellular transplant. Methods ?2m-/Thy-1+ BDLSCs were isolated by magnetic bead cell sorting(MACS) method from cholestatic rats in vitro,and cell purity was detected using flow cytometry.Liver associated phenotype markers were characterized by RT-PCR and immunofluorescence staining. Results BDLSCs isolated by MACS were purified and viable,and possessed hepatocyte-like features at gene and protein levels. Conclusion ?2m-/Thy-1+ BDLSCs are special subsets of BMSCs which may have promising potentials in the stem cell-based treatment of liver diseases.

Objective To observe the effect and safety of the human glucagon-like peptide-1 analogue,liraglutide,versus insulin glargine in patients with type 2 diabetes mellitus inadequately controlled with metformin alone.Method Ninty patients with type 2 diabetes mellitus(aged 18-79 years,HbA1C 7.5％-10.0％,body mass index＜40 kg/m2) who had inadequate glycaemic control on metformin were allocated for the research with an open,randomized,parallel controlled clinical research method.The patients kept the original dose of metformin unchanged and were randomly assigned to the liraglutide group or the insulin glargine group according to a proportion of 1 ∶ 1.Liraglutide group started with a dose of 0.6 mg subcutaneous injection qd,changed to 1.2 mg subcutaneous injection qd after one week and kept unchanged until the end of the research.Insulin glargine group started with a dose of 0.1-0.2 U/kg according to the fingertips peripheral blood glucose level before breakfast on the continuous 3 d before every follow-up.At the baseline,after 4 weeks,12 weeks,20 weeks,and 26 weeks of treatment,HbA1C,blood glucose,lipids weight,blood pressure were arranged to measured.86 patients finally completed the study.Results Mean HbA1C and the success rate of HbA1C ＜7％ were similar between liraglutide group and insulin glargine group [(7.06 ± 0.87) ％ vs (7.25 ± 1.20) ％,47.73 ％ vs 45.23 ％,P＞0.05],while the percentage of subjects reaching the composite endpoint of HbA1C＜7％ with no hypoglycemia and no weight gain was significantly higher in liraglutide group than insulin group(P＜0.05) ; Fasting plasma glucose decreased more markedly in insulin glargine group,2 h postprandial plasma glucose was decreased more markedly in liraglutide group(P＜0.05 or P＜0.01).Liraglutide significantly reduced mean body weight by (3.21 ± 1.18) kg,waist circumference by (3.82 ± 1.21) cm,and body mass index by (1.95 ± 0.61) kg/m2 (P＜0.01 or P＜0.05),while in the insulin glargine group there sere rise of respective figure of(2.86 ± 0.43) kg,(1.52 ± 0.56) cm,and (0.61 ± 0.25) kg/m2 (P＜0.05),systolic blood pressure and serum triglyceride declined.There was no serious adverse affect in both groups,the incidence of mild hypoglycemia was significantly less in liraglutide group and has a statistically significant difference (4.55％ vs 21.43％,P＜0.05).Conclusions Liraglutide showed a good effect on reducing weight,systolic blood pressure,blood lipid and in addition to blood glucose control which is comparable to insulin glargine.What is more,liraglutide had good safety and tolerability,which can be regarded as a good choice for patients with type 2 diabetes mellitus inadequately controlled with metformin alone.

Background and purpose:Colorectal cancer is one of the most common cancers worldwide as well as a prevalent cause of morbidity and mortality in China.Mustard seed and its components have been reported to possess anticancer properties.The purpose of this study was to investigate the effect of a diet mixed with mustard seeds (MS)on the prevention of colorectal tumorigenesis in Kunming mice induced with azoxymethane.Methods:Sixweek-old female Kunming species mice of were randomly divided into 4 different groups:AOM alone,AOM+5%MS,AOM+10%MS,and the untreated control group.Colorectal tumorigenesis was subcutaneously induced by injecting 10 mg/kg of AOM once a week,for 3 weeks.Different doses of MS were administered in the diet during this study.Thirtytwo weeks after initiation,the mice were put down through cervical dislocation.The colon and recta of the mice were isolated and flushed with nonnal but ice-cold saline.The site,size,and number of colorectal tumors were recorded.The incidence of colorectal tumor in each group was determined.Results:No tumors were found in the control group.Compared to AOM-treated control group(86.7%),the occurrence of colorectal tumors was reduced to 60.0%when administered with a 5%diet of mustard seeds and to 41.7%(P＜0.05)with a diet of 10%mustard seeds.Tumor burden (tumors/mouse)was 2.2±1.2 in the AOM-treated control group whereas it was reduced to 1.1±1.1(P＜0.05)in the 5%diet of mustard seeds and 0.7±0.9(P＜0.05)in the 10%diet of mustard seeds.Conelusion:The results indicate that a diet of mustard seeds is able to inhibit the development of colorectal cancer in mice induced by AOM,suggesting possible future application of MS as a chemopreventive agent for colorectal cancer.

Objective To investigate the association of single nucleotide polymorphism (SNP) rs13333226 in uromodulin (UMOD) gene with diabetic kidney diseases (DKD) in Han population in Tianjin,China.Methods A total of 210 type 2 diabetes (T2DM),90 normal controls (NC) and 280 DKD patients were recruited.According to the level of estimated glomerular filtration rate (eGFR),the DKD subjects were further subdivided into three groups:GFR≥90 ml/min group (n=105),60 ml/mim≤GFR ＜ 90 ml/min group (n=84) and GFR ＜ 60 ml/min group (n=91).Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) was used for UMOD rs13333226C genotyping.Results The frequencies of AA,GA,GG genotype were 27.8％,58.9％,13.3％ in NC group and 41.0％,48.6％,10.5％ in T2DM group and 54.3％,36.1％,9.6％ in DKD group.The frequency of G allele was 42.8％ in NC group,34.8％ in T2DM group and 27.7％ in DKD group.The genotype distribution of UMOD was statistically significant between NC group and DKD group,and between T2DM group and DKD group (P ＜ 0.05).G allele of UMOD was an independent protective gene polymorphism of DKD in Logistic regression (B=-0.248,Wald=8.012,P=0.021,OR=0.780,95％ CI 0.612-0.968).Conclusion The G allele of UMOD gene may be an independent protective factor of DKD in Han population in Tianjin,China.

Objective To investigate facial-cervical index difference in undergraduates of different height or weight in Han nationality to provide an additional reference for plastic surgery of facialcervical region.Methods Subjects consisted of 200 college undergraduates of Han nationality,aged 20 to 24 years,with average 22.9 ± 1.7,of whom 100 were male and 100 were female.Their height,weight,bizygomatic breadth,facial height,cervical height,neck circumference,neck breadth,shoulder width,cervical-mandibular angle,thorax-cervical angle,shoulder-cervical angle,pulvinar-cervical angle and back-cervical angle were measured and calculated.Results The differences among the indices mentioned above were of statistical significance (P＜0.05);the indices of female subjects of different height were significantly different (P＜0.05);the differences in indices of male subjects of different weight were of statistical significance (P＜0.05);the indices of female subjects of different weight were also statistically different (P＜0.05).The facial-cervical indices in both male and female subjects of different height or weight were of no stistical difference (P＞0.05).Conclusions Male subjects of different height or weight have different cervical height,cervical-mandibular angle,neck height and facial height indices are different;hence,the indices are related to body height;female subjects of different body height have different neck height.Subjects of different weight have different neck circumference,neck breadth,should breadth,shoulder-cervical angle and pulvinar-cervical angle.