AIM: To investigate the effect of binocular vision training on stereopsis establish and visual function in child concomitant strabismus after surgery
METHODS:Ninety-three cases of strabismus children were randomly divided into two groups, making it comparable. The control group (n=46) were treated with conventional surgery and care;the observation group ( n=47) on the basis of routine care as control group, were given binocular vision training. Synoptophore and Titmus stereoscopic view were used to check the function of stereoscopic.
RESULTS:After treatment, simultaneous perception, visual fusion and stereopsis of trail patient were 77%, 62% and 40%, respectively, those of control group were 48%, 35% and 18%, the difference was statistically significant (P<0. 05). After treatment, fovea stereopsis and macular stereopsis children in observation group were accounted for 32% and 26%, respectively, in the control group those were accounted for 18% and 11%, respectively. Those in the observation group were significantly more than the control group. No stereoscopic patients in observation group were 23%, and that was 54% in the control group. That in the observation group was significantly less than the control group. The difference comparison between the two groups was statistically significant (P<0. 05).
CONCLUSION: Binocular vision training can effectively help children to build concomitant strabismus after surgery, for children with strabismus should be treated with conventional visual training to improve visual function and improve the life quality.

Child ; Child, Preschool ; Gleditsia ; chemistry ; Humans ; Infant ; Male ; Moxibustion ; Penile Diseases ; therapy ; Seeds ; chemistry

Objective To investigate the characteristics,the incidence, relative factors of antibiotic associated diarrhea (AAD),and prevent and control it. Methods Retrospective analysis was performed in 78 patients with AAD. Results Prevalence ratio of AAD was 9.3%, the risk of AAD related to the kinds of using mix antibiotics and period of treatment, using many meddle measures for medical and age.The antibiotics causing AAD were extending penicillin or third cephalosporins, penicillin, carbapenemases, second cephalosporins.Conclusions Rational use of antibiotic,control use of antibiotic and reduce the state of consciousness are the key measures to prevent and control the AAD.

Objective To evaluate the hemodynamic effects of rocuronium in patients with rheumatic heart disease during induction of anesthesia.Methods Twenty patients of either sex (ASA classes Ⅲ -Ⅳ ;NYHA classes Ⅱ -Ⅲ) scheduled for valve replacement were included in this double blinded study. Their ages ranged from 34 to 62 yr and weights from 48 to 70 kg. The patients were premedicated with intramuscular morphine 0.2 mg?kg-1 and scopolamine 0.3mg. Radial artery was cannukted for direct BP monitoring and Swan-Ganz catheter was placed via right internal jugular vein for hemodynamic monitoring before induction of anesthesia.TOF,BIS,ECG and SpO2 were also monitored throughout anesthesia. Anesthesia was induced with midazolam 0.05-0.1 mg?g-1 and fentanyl 10-15?g?kg-1. The patients were randomized to receive either rocuronium 0.6 mg?kg-1 (group Ⅰ,n=10) or vecuronium 0.1 mg?kg-1(group Ⅱ,n=10) to facilitate tracheal intubation when BIS value dropped to 60%.The patients were mechanically ventilated (VT 8-10 ml?kg-1 ,RR 10-12 bpm).Systolic arterial pressure (SAP),MAP,HR,cardiac output (CO), PCWP, CVP, mixed venous blood O2 saturation (SvO2), BIS and TOF were recorded and stroke volume (SV), stroke index (SI), LVSWI and rate-pressure product (RPP) were calculated before anesthesia (To), 1 min after administration of muscle relaxant (T1), when TOF reached 0 (T2) and 1,2,3,4,5,7,10,15,20,25,30 min after tracheal intubation (T3-13).Results The demographic data including age, sex and body weight were comparable between the two groups. In rocuronium group HR increased by 17.43% -7.54%,SAP increased by 16.94% - 12.3% and RPP increased by 13.96% - 22.67% respectively during 1-7 min after intubation (T3-8) as compared with the baseline values (To), significantly higher than those in vecuronium group (P

AIM :To investigate the impact of alendronate on bone resorption of normal and ovariectomized rats at the bone-screw interface through radiographic and histologic findings.METHODS :Thirty-two female Wister rats with a mean body weight of 332 g(287-351 g)were divided into four groups at random.Rats in group C and D were ovariectomized.8 weeks later,the proximal one-third of the left tibia of all rats were inserted into self-drilling mini cortical screws.After operations,alendronate were used in group A and C and saline were given in group B and D.The rats were euthanized at 5 weeks after screws having been attached.Radiographic and histologic findings subsequently were analyzed.RESULTS :Radiographs confirmed that no osteolytic area was present around screws immediately after insertion,whereas 5 weeks after insertion,a wide and low-density area corresponding to the screw hole was evident in the saline groups compared with the alendronate groups.On histologic observation,the width of the fiber membrane and the number of TRAP-positive cells were decreased in the alendronate groups than those in the saline groups,and the difference was statistically significant.CONCLUSION :Alendronate effectly inhibits bone resorption of either normal or ovariectomized rats at the bone-screw interface in rats.

Lornoxicam is a new non-steroidal anti-inflammatory drug,it can inhibit the activity of cyclooxygenases and the immunologic injury afteroperation.Its efficacies on preoperative,operative period and postoperative analgesia are similar to those of opioid.Lornoxicam can decrease the dosage of opioid drugs and has the good qualities of short half-life,few adverse effects and satisfactory tolerance.Lornoxicam can provide rapid and stable analgesic effect in perioperative phase.

Objective:To study the therapeutic effect of 2 artificially synthesized fusion peptides on scalded mice. Methods: Two new fusion peptides, M-T508 and M-T?1, were synthesized based on amino acid sequences selected according to the characteristics of scald (including 3 stages: inflammation, infection and repair by cell proliferation). The 2 peptides were applied for second degree scald burn in mice and the healing of the scald burn was observed. The expression ofⅠcollagen, vascular endothelial growth factor (VEGF), proliferating cell nuclear antigen (PCNA), and cytokeratin (CK) was detected by immunohistochemical staining. Serum TNF-? concentration was determined by ELISA in the animals and the blood vessel permeability was analyzed by Evans blue staining. Results: The healing periods of scald burns in the 2 peptide groups were 1 week earlier than that in the control group. Immunohistochemical results showed that the expression of collagenⅠ, VEGF, PCNA and CK was all positive in the 2 peptide groups 10 days after scald and negative in the control group. Examination of blood vessel permeability and serum TNF-? concentration in the M-T?1 and M-T508 groups showed that the 2 fusion peptides had anti-inflammation and anti-infiltration effects in scalded mouse. Conclusion: The synthesized fusion peptide M-T?1 and M-T508 can promote the healing of scald burns in mice.

Objective To investigate the induction of specific anti-tumor immune response by transfected dendritic cells (DCs) with survivin mRNA of human pancreatic cancer, and to provide the experimental evidences for the treatment of human pancreatic cancer with DCs vaccine. Methods DCs were isolated and cultured from peripheral blood mononuclear cells (PBMCs). After being transcripted and amplified, survivin mRNA was transfected into DCs by electroporation. The expression of survivin in DCs at different time points was detected by quantitative real-time PCR. The survival rate of DCs before and after transfection was determined by MTT method. The induction of specific cytotoxic T lymphocyte (CTL) response by survivin mRNA transfected DCs was measured by 51Cr standard cytotoxicity test. The induction of specific CTL activation by survivin mRNA transfected DCs was evaluated through testing released IFN-γ by ELISA method. Results After survivin mRNA transfection for 48h, the expression of survivin mRNA in DCs reached the highest point (46.09±6.57). After transfection, the survival rate of DCs was stabilized around 80%. The DCs transfected with survivin mRNA could effectively induce HLA-A2+ / survivin+ specific CTL immune responses. Stimulated with pancreatic cancer cell line Capan-2 cells or SCL-1 cells as control group, the IFN-γ released in 24 hours by survivin specific CTL were (28.79±5.70) U/ml and (25.12±2.13) U/ml respectively, there was no significant difference (P=0.761). Conclusion The induction of CTLs by DCs transfected with human pancreatic cancer survivin mRNA could produce specific anti-tumor immunity.

BACKGROUND:Previous studies have found that embryonic bone marrow mesenchymal stem cel s can promote human Th17 cel proliferation, but the inherent regulatory mechanisms stil need to be elucidated. OBJECTIVE:To investigate the role of Tol-like receptor 3 in the immunoregulation of Th17 cel s by mesenchymal stem cel s.
METHODS:Human CD4+T cel s from healthy donors were isolated by immunomagnetic bead method, and then cultured alone or co-cultured with embryonic bone marrow mesenchymal stem cel s for 4 days. The mRNA expression level of interleukin-17, Tol-like receptor 3 and MyD88 was detected by real-time PCR.
RESULTS AND CONCLUSION:Compared with CD4+T cel cultured alone group, the mRNA level of interleukin-17 was significantly higher in the co-culture group (3.59±0.11 vs. 1.14± 0.08, P<0.01). Consistent with the expression of interleukin-17 mRNA, increased level of Tol-like receptor 3 mRNA was detected in the co-culture group compared with the CD4+T cel cultured alone group (3.10±1.60 vs. 0.94± 0.01, P<0.05). Furthermore, MyD88 in the co-culture group was significantly higher than that in CD4+T cel cultured alone group (2.29±0.05 vs. 1.85±0.31, P<0.01). Tol-like receptor 3 may be involved in the immunoregulation of Th17 cel s by embryonic bone marrow mesenchymal stem cel s, which provides experimental evidence for potential cel therapeutic strategy.

Objective To investigate the pyschological states and behavior habits of school-age children with cleft of lip and /or palate .Methods 50 children at the age of 8-12 with cleft of lip before the first operation and their parents and 50 normal control children were tested with SAS (social anxiety scale) ,SEC(self esteem scale) ,and CBCL(children behavior checklist) .Results The rate of abnormal tested by CBCL (children behavior checklist)-parent in patients was 22% ,was higher than that in normal 8% (χ2 =3 .85 ,P<0 .05) .The scores of SAS in patients was higher than those in control (t=5 .29 ,P<0 .01) .Conclusion School-age chil-dren with cleft of lip and/or palate had obvious psychological and behavioral problems ,thus ,corresponding nursing and health edu-cation should be applied .