1.DNA sequences of the HLA-DRB1 chain from HLA-DR4 subtype alleles.
Mun Gan RHYU ; Byung Uk LIM ; Gum Ryong KIM
Korean Journal of Immunology 1993;15(1):119-127
No abstract available.
Alleles*
;
Base Sequence*
;
DNA*
;
HLA-DR4 Antigen*
;
HLA-DRB1 Chains*
2.Isolation of hemorrhagic efver with renal syndrome virus from peripheral blood leukocytes of patients.
Gum Ryong KIM ; Tai Gyu KIM ; Suk Do KIM ; Jin Chul SHIN
Journal of the Korean Society of Virology 1991;21(2):141-149
No abstract available.
Humans
;
Leukocytes*
3.Detection of human immunodeficiency virus-1 and -2 DNA in seropositive Koreans by two-step polymerase chain reaction and hybridization with digoxigenin-probes.
Tai Gyu KIM ; Hoon HAN ; Gum Ryong KIM ; Chun KANG ; Yung Oh SHIN
Journal of the Korean Society of Virology 1992;22(1):81-90
No abstract available.
DNA*
;
Humans*
;
Polymerase Chain Reaction*
4.Loss of heterozygosity at the MCC and APC genetic loci in precancerous gastric lesion and gastric cancer.
Mun Gan RHYU ; Won Sang PARK ; Yuen Jun JUNG ; Gum Ryong KIM ; Choo Soung KIM
Journal of the Korean Cancer Association 1992;24(5):695-701
No abstract available.
Genetic Loci*
;
Loss of Heterozygosity*
;
Stomach Neoplasms*
5.The Rsa I polymorphic site within exon II of APC(adenomatous polyposis coli) gene determined by digestion of PCR products in human gastric cancer.
Mun Gan RHYU ; Won Sang PARK ; Gum Ryong KIM ; Choo Soung KIM
Journal of the Korean Society for Microbiology 1992;27(2):189-196
No abstract available.
Digestion*
;
Exons*
;
Humans*
;
Polymerase Chain Reaction*
;
Stomach Neoplasms*
6.Correlation of Virologic Property and Phylogenetic Analysis of Hantaan Viruses Isolated from Patients and Reservoirs in Korea.
Yong Tae JUNG ; Sun Ryang LEE ; Soon Young PAIK ; Gum Ryong KIM
Journal of the Korean Society of Virology 1998;28(1):11-20
Twenty isolates of Hantavirus were isolated from patients and reserovirs from 1988 to 1994 in Korea. Isolation rate was 1.9% (10/538) in patients, 6.2% (5/81) in Apodemus sp., 2.6% (1/38) in Rattus sp. and 0.6% (4/677) in bats. Reciprocal mean IFA titers ranged from 27.5 to 1,024 at the specimen collection. According to the growth rate and reaching peak titer of infectivity, the isolates were grouped as rapid, intermediate, and slow growing groups. All isolates were confirmed as Hantaan type by the nested RT-PCR on the Gl region of the M segment. Comparison of nucleotide sequence (Nt: 2101 - Nt: 2280) of the G2 region revealed that the sequence homology between Hantaan 76/118 virus and the isolates was more than 90%. Several nucleotide positions of the isolates showed high variation. The variation rate of patientisolates was about one-half when compared with that of rodentisolates. On the basis of phylogenetic analysis Hantaan viruses isolated were divided into two genogroups. These results indicate that Hantaan virus is highly dominant serotype in Korea and the virologic property and genogroup are not correlated.
Animals
;
Base Sequence
;
Chiroptera
;
Genotype
;
Hantaan virus*
;
Hantavirus
;
Humans
;
Korea*
;
Murinae
;
Rats
;
Sequence Homology
;
Specimen Handling
7.Reverse hybridization between non-tailed probes and sample DNA labeled with non-radioactive hapten: a simple and rapid methodrecommended for HLA-DR typing using PCR.
Mun Gan RHYU ; Tai Gyu KIM ; Yeun Jun CHUNG ; Seon Young KIM ; Gum Ryong KIM ; Hoon HAN
Korean Journal of Immunology 1991;13(2):187-194
No abstract available.
DNA*
;
HLA-DR Antigens*
;
Polymerase Chain Reaction*
8.Mutation of Adenomatous Polyposis Coli Gene in Human Stomach Cancer.
Won Sang PARK ; Mun Gan RHYU ; Sug Hyung LEE ; Yun Jun CHUNG ; Gum Ryong KIM ; Choo Soung KIM
Korean Journal of Pathology 1993;27(1):34-39
Recently the adenomaatous polyposis coli(APC) gene, a tumor suppressor gene, was identified and the cDNA was cloned from chromosome 5q21. Allelic deletion or point mutation of tumor suppressor genes(TSGs) has been considered as an important mechanism in development of human tumor. Point mutations affecting APC gene are seen in the hereditary syndrome, adenomatous polyposis and spordic colon cancer. However, the mutation of APC gene and other TSGs have not been described in gastric cancer. In order to identify the mutation of exon 11 of APC gene for gastric cancer, we amplified DNA extracted from paraffin-embedded tissues by polymerase chain reaction(PCR) and digested the PCR products with restriction enzyme Rsa I. We examined the DNA extracted from paraffin-embedded 44 gastric cancer tissues with lymph nodes. Eighteen(41%) among 44 were informative for the study exon 11 of the APC gene, and we found loss of heterozygosity(LOH) for APC in 6/18(33.3%). These data suggest that the point mutation or the base change of APC gene commonly occurs in gastric cancer. We conclude that the mutation of APC gene is strongly connected with development of human gastric cancer.
Humans
;
Genes, Tumor Suppressor
;
Stomach Neoplasms
9.Rapid detection of human cytomegalovirus(HCMV) in urine from kidney transplant patients by polymerase chain reaction(PCR) and hybridization.
Tai Gyu KIM ; Moon Won KANG ; Wan Shik SHIN ; Mun Gan RHYU ; Yeun Jun JUNG ; Hoon HAN ; Gum Ryong KIM
Journal of the Korean Society for Microbiology 1992;27(1):79-86
10.Rapid detection of human cytomegalovirus(HCMV) in urine from kidney transplant patients by polymerase chain reaction(PCR) and hybridization.
Tai Gyu KIM ; Moon Won KANG ; Wan Shik SHIN ; Mun Gan RHYU ; Yeun Jun JUNG ; Hoon HAN ; Gum Ryong KIM
Journal of the Korean Society for Microbiology 1992;27(1):79-86
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