Objective To observe the adverse effects of two different high-dose methotrexate(MTX) in pediatric acute lymphoblastic leukemia.Methods Totally 25 cases acute lymphoblastic leukemia children were grouped according to the risk factor,the low-risk group(13 patients) received MTX 3 g/m2 continuous infusion and high-risk group(12 patients) received MTX 5 g/m2 continuous infusion.Using Calcium Folinate(CF) 36 h after first dose MTX (1/6 total dose) ,then compare the incidence rates and the severity of the MTX of adverse effects.Results Gastrointestinal syptoms, hver damage, mucosal damage were the most common adverse effects after high-dose MTX infusion, the two groups had no significant difference (P ＞ 0.05).Conclusion The incidence rates and the severity of adverse reactions of high dose MTX were related directly with the level and the persistencetime of the plasma concentration.

OBJECTIVETo investigate whether the variants A(-6)G and A(-20)C of angiotensinogen (AGT) gene are involved in the pathogenesis of essential hypertension in Kazakans.

METHODST his case control study recruited 125 subjects with hypertension and 74 normotensive subjects from Kazakans of Xinjiang. Genomic DNA from leukocytes was analyzed for genetic variants A(-6)G and A(-20)C in 5' upstream core promoter of AGT gene by polymerase chain reaction (PCR), single strand conformation polymorphism (SSCP), restriction fragment length polymorphism (RFLP) and automatic sequencing.

RESULTS(1)There were only A(-6)G and A(-20)C variants in the -164 to +73 region of Kazakans' AGT gene. (2) The distributions of genotypes AA, AG, GG at locus -6 of AGT gene showed significant difference between the hypertensive group (0.39, 0.45, 0.16) and the normotensive group (0.49, 0.49, 0.02; Chi2=8.56, P=0.014). There were evident differences in the frequencies of the -6A and the -6G allele of the two groups (0.62, 0.38 and 0.73, 0.27; Chi2=5.35, P=0.021). (3) No significant difference was observed in the distribution of genotypes AA, AC, CC at locus -20 of AGT gene between the hypertensive group (0.69, 0.26, 0.05) and the normotensive group (0.65, 0.32, 0.03; Chi2=2.42, P=0.30). There was no distinct difference in the frequencies of the -20A allele and the -20C allele of the two groups (0.82, 0.18 and 0.82, 0.18; Chi2=0, P=0.99). (4) No significant difference was found at the levels of systolic and diastolic blood pressure between the groups corresponding to genotypes at the loci -6 and -20 of AGT gene.

CONCLUSIONThe results suggest that the polymorphism of A(-6)G in 5' upstream core promoter of the AGT gene may be involved in the pathogenesis of essential hypertension in Kazakans, while the A(-20)C variant may not play an important role in the etiology of essential hypertension in Kazakans.

5' Flanking Region ; genetics ; Adult ; Alleles ; Angiotensinogen ; genetics ; Base Sequence ; Blood Pressure ; physiology ; Case-Control Studies ; China ; DNA ; chemistry ; genetics ; DNA Mutational Analysis ; Female ; Gene Frequency ; Genotype ; Humans ; Hypertension ; genetics ; physiopathology ; Male ; Middle Aged ; Molecular Sequence Data ; Polymorphism, Genetic ; Polymorphism, Single-Stranded Conformational ; Promoter Regions, Genetic ; genetics