OBJECTIVE:To study the effects of Xingnaojing injection on the expressions of both the protein of IL-1? and the mRNA in the experiment intracerebral hemorrhage rats.METHODS:Referenced by Zou's method and Rosengerg method,rat intracerebral hemorrage model was established and the model rats were then divided into normal group,model group and Xingnaojing group.The pathological changes in all the groups were observed and the expressions of the protein of IL-1? and the mRNA were detected after medication.RESULTS:In Xingnaojing group as compared with model group,the cells arranged regularly,the edema degree was lessened,the inflammatory cells were less;the disorganization of plastiosome was less severe,synaptic structure was clearer.The expressions of both the protein of IL-1? and the mRNA were all lower than in the model group.CONCLUSION:Xingnaojing injection can relieve the cerebral edema and inhibit the expression of IL-1? of the brain tissue surrounding the hematoma in intracerebral hemorrhage rats.

Objective To construct pSUPER-SVV vector and obtain its expression in HeLa cells,in order to interfere the expression of survivin.Methods Oligonucleotide sequences specific for survivin were designed for RNA interference(RNAi),after annealed they were ligated to pSUPER.basic vector.After sequencing,the vector of pSUPER-SVV was transfected into HeLa cells by LipofectAmine.The expression of survivin was detected by RT-PCR and FCM.Results The RNAi expression vector of survivin was constructed successfully,which depressed the expression of survivin efficiently.Survivin was significantly depressed on the level of mRNA in HeLa cells compared with the control groups(P

Objective To investigate the levels of complement and activated complement factors in peripheral blood and skin in patients with psoriasis and their significance in the development of psoriatic lesion.Methods Enzyme linked immunosorbent assay (ELISA) and immuno turbidi metric analysis were used to determine the levels of C3d, sC5b-9, C3 and C4 in sera from 57 patients with psoriasis vulgaris.Immunohistochemistry was employed to assess in situ expression of SC5b-9 in lesional and non lesional skin of 37 patients.Results There was a significant decrease of C3 and C4, and increase of C3d and sC5b-9 in patients with psoriasis in comparison with those of normal controls (t=10.88～ 24.80, P<0.01).The levels of C3 and C4 were significantly lower, while the levels of C3d and sC5b-9 were significantly higher in progressive stage than those in static stage (t=4.55～ 6.77,P< 0.01).In situ expression of sC5b-9 was significantly higher in stratum corneum and dermo epidermal junction of lesional skin than that in non lesional skin and normal controls (χ2=62.90,45.45;P< 0.01).There was no significant difference of C5b-9 expression in stratum corneum and dermoepidermal junction of lesional skin between progressive and static stage.Conclusions There is significant correlation between the development of psoriatic lesion and local deposit of activated complement factors.

Loss of the ethical basis in the Chinese healthcare reform is embodied in a number of noticeable questions arising during the reform and development course.We should adhere to correct value orientation and ethical principles in deepening healthcare reform.Each stakeholder inside the reform including government,medical institutions,medical staff,patients and their relatives should take their respective ethical responsibilities.Therefore,the Chinese healthcare industry could develop with Chinese unique characteristics,and the healthcare reform will conduct correctly and continuously.

Objective To investigate the levels of complement and activated co mplement factors in peripheral blood and skin in patients with psoriasis and the ir significance in the development of psoriatic lesion. Methods Enzyme linked im munosorbent assay (ELISA) and immuno turbidi metric analysis were used to dete rmine the levels of C3d, sC5b－9, C3 and C4 in sera from 57 patients with psori asis vulgaris. Immunohistochemistry was employed to assess in situ expression of SC5b－9 in lesional and non lesional skin of 37 patients. Results There was a significant decrease of C3 and C4, and increase of C3d and sC5b－9 in patient s with psoriasis in comparison with those of normal controls (t=10.88～ 24.80, P

Objective To explore the correlation between the leukocyte rheology, cell adhesion molecule(CAM) expression and psoriasis. Methods Enzyme linked immunosorbent assay(ELISA), erythrocyte deformability apparatus and in vitro thrombus and platelet adhesion dual purpose apparatus were used to determine the changes of leukocyte CD18 expression, levels of serum soluble intercelular adhesion molecule 1(sICAM 1), and the changes of leukocyte filtration index(LFI) and leukocyte adhesion rate(LAR). Results The leukocyte CD18 expression, sICAM 1 level, LFI and LAR were significantly increased in patients with psoriasis than those in controls(P

OBJECTIVE: To investigate the clinical application value of cone-beam CT (CBCT) online correcting technology in volume modulated radiation therapy (VMRT) for nasopharyngeal carcinoma (NPC). METHOD: Fifty NPC patients applying head neck and shoulder thermoplastic body membrane fixing device were eligible for treatment VMRT, these patients would accept a couple of CBCT scanning by on board imager (OBI) in a fixed time each week after initial setup and after online correcting during the first three week for radiotherapy, CBCT images and DRR images constructed by CT simulation were carried out registration,which could calculate the setup errors of initial setup and after online correcting. RESULT: Fifty patients were accepted 150 scanning after initial setup and 150 scanning after online correcting respectively, the errors after initial setup were (-1.24 ± 1.25)mm in X direction, (1.19 ± 1.85)mm in Y direction, (1.49 ± 1.70) mm in Z direction. The setup errors after online correcting were (-0.13 ± 0.29)mm in X direction, (0.10 ± 0.47)mm in Y direction, (0.17 ± 0.36)mm in Z direction. The setup errors after online correcting were significantly lower than the errors after initial setup in X direction (P < 0.05), Y direction (P < 0.05), Z direction(P < 0.05). The M(PTV) value after online correcting were 0.46 mm, 0.53 mm and 0.59 mm in X, Y and Z directions respectively. CONCLUSION The use of CBCT online correcting technology can significantly reduce the setup errors of VMRT for NPC and improve the treatment effect.
Carcinoma ; Cone-Beam Computed Tomography ; Humans ; Nasopharyngeal Carcinoma ; Nasopharyngeal Neoplasms ; radiotherapy ; Radiotherapy Planning, Computer-Assisted ; Radiotherapy, Intensity-Modulated

Objective To study the change of chromosome aberration rate and micronucleus rate in patients with esophageal carcinoma before and after radiotherapy.Methods Twenty one patients with esophageal carcinoma who were fit for conditions were divided into two groups randomly.GroupⅠ(n=10),patients received conventional radiotherapy(radiation field was regular rectangular shape);groupⅡ(n=11),patients received conformal radiotherapy(radiation field was same to the shape of target volume),1.8-2.0 Gy,one time a day,5 times a week,the total dose was 60-70 Gy.The peripheral blood was obtained before and after radiotherapy,chromosome aberration rate and micronucleus rate were detected.Results The chromosome aberration rate and micronucleus rate were increased after conventional radiotherapy than before (P0.05).Conclusion Compared with the conventional radiotherapy,the conformal radiotherapy can decrease cytogenetics damage,the detection of chromosome aberration rate and micronucleus rate can be regarded as a simple method to evaluate cytogenetics damage in esophageal carcinoma patients induced by radiotherapy.

Objective To approach the correlation between the bone density measured by quantitative CT and the blood sugar level of the aged patients with non-insulin-dependent diabetes mellitus,and observe the effects of the blood sugar level on the bone density.Methods The lumbar bone densities and the blood sugar levels of 160 aged patients with non-insulin-dependent diabetes mellitus (hyperglycemia group 80 cases,euglycemia group 80 cases ) and the healthy aged people (80 cases) were detected by quantitative CT and serum biochemical detection; the correlation between the blood sugar level and the bone density and the osteoporosis occurrence status of aged people in various groups were analyzed.Results The bone density in the non-insulin-dependent diabetes and hyperglycemia group was lower than those in normal(control) group and non-insulin-dependent diabetes and euglycemia group (P

Objective:To explore the mediating effect of self-identity on the relationship between interpersonal adaptation and the tendency of mobile phone addiction in college students.Methods:Totally 498 college students (217 males and 281 females) were selected and assessed with the College Student Interpersonal Adaptability Inventory (CSIAI),Mobile Phone Addiction Tendency Scale (MPATS) and Personal Identity Scale(PIS).The bias-corrected percentile bootstrap method was used to analyze the mediating effect of self-identity between interpersonal adaptation and mobile phone addiction tendency in college students.Results:The MPATS scores of male students were significantly lower than female students [(2.6 ± 0.7) vs.(2.7 ± 0.6),P ＜ 0.05].The MPATS scores were negatively correlated with the scores of CSIAI and PIS (r =-0.31,-0.36,Ps ＜ 0.01),the CSIAI scores were positively correlated with the PIS scores (r =0.50,P ＜0.01).Self-identity played a partly mediating role between interpersonal adaptation and mobile phone addiction tendency,which accounted for 43.6％ of the total effect.Conclusion:It shows that the mobile phone addiction tendency may be related to interpersonal adaptation and self-identity,interpersonal adaptation could affect the mobile phone addiction tendency through the self-identity.