Recently the studies on mosquito genomics, transcriptomics and small RNAomics developed rapidly with the novel biotechnologies of the next generation sequencing techniques. The genome sequences of several important vector mosquitoes including Anopheles gambiae, Culex quinquefasciatus, and Aedes aegypti have been published. The genome sizes vary among the different species of mosquitoes and are consistent with the number of the repeat regions. The released genome sequences facilitate gene cloning and identification as for OBP, OR and dsx genes. Transcriptomics provides a useful tool for functional analyses of the mosquito genes, and using this technique, the molecular basis of mosquito blooding, gland proteins and diapauses have been explored. Studies on small RNAomics suggest important roles of miRNAs and piRNAs in ovary development, blood digestion, and immunity against virus infection. The studies on mosquito omics have generated a big data platform for investigation of vector biology and vector-transmitted disease prevention.
Aedes ; genetics ; Animals ; Anopheles ; genetics ; Culex ; genetics ; Gene Expression Profiling ; Genome, Insect ; Genomics ; High-Throughput Nucleotide Sequencing ; MicroRNAs

Objective Analyze the characteristics of delayed apheresis platelet donors due to health consultation or ex-amination,and the influencing factors of recall in Guangzhou,and to provide evidence for recruitment strategy.Methods 5 960 delayed apheresis platelet donors from January fisrt,2022 to June 30th,2022 were collected in Guangzhou Blood Center and fol-lowed up to August31th,2023.Logistic regression analysis was used to analyze the influence of sex,age,times of blood donation and length of blood donation on recall.Results Among the 5 960 delayed platelet donors,164(2.75%)were permanently un-suitable for blood donation.The most common reason was allergy.Among the 5 796(97.25%)delayed blood donors,the most common reason was abnormal blood routine.Analysis of factors associated with delayed donor recall found that male donors were more likely than female donors[OR =1.16,95%CI(1.01,1.34)],blood donors aged 26-35[OR =1.65,95%CI(1.41,1.92)],36-45[OR=1.90,95%CI(1.55,2.31)]or 46-60[OR =2.63,95%CI(1.96,3.53)]compared with those aged 18-25,blood donors who were blocked forless than 14 days[OR =4.22,95%CI(2.73,6.52)]orfor 15-89 days[OR =5.24,95%CI(3.42,8.03)]compared with those who were blocked for more than or equal to 90 days,the recall rate of repeat donors was higher than that of first-time donors[OR=6.78,95%CI(5.62,8.19)].Conclusion In order to improve the recall rate of apheresis platelet donors,we should pay more attention to the education of young donors and first-time donors,also consider a voluntary recall by text message or phone after a donor has been prevented from donating blood.

Recently the studies on mosquito genomics, transcriptomics and small RNAomics developed rapidly with the novel biotechnologies of the next generation sequencing techniques. The genome sequences of several important vector mosquitoes including Anopheles gambiae, Culex quinquefasciatus, and Aedes aegypti have been published. The genome sizes vary among the different species of mosquitoes and are consistent with the number of the repeat regions. The released genome sequences facilitate gene cloning and identification as for OBP, OR and dsx genes. Transcriptomics provides a useful tool for functional analyses of the mosquito genes, and using this technique, the molecular basis of mosquito blooding, gland proteins and diapauses have been explored. Studies on small RNAomics suggest important roles of miRNAs and piRNAs in ovary development, blood digestion, and immunity against virus infection. The studies on mosquito omics have generated a big data platform for investigation of vector biology and vector-transmitted disease prevention.

【Objective】 To obtain HTLV prevalence data among blood donors in mainland China through meta-analyze, and provide reference for relevant policies. 【Methods】 The literature concerning HTLV prevalence among blood donors in mainland China before April 15, 2021 in CNKI, Wanfang and Pubmed was searched. According to the inclusion criteria and exclusion criteria, relative literature within recent 10 years was screened and then analyzed by meta-analysis using R3.3. 【Results】 A total of 69 articles were included, covering 22 provincial administrative regions, with a total sample size of 7 435 501 cases. 535 cases were HTLV positive, of which 491 centred on three coastal provinces as Fujian, Guangdong and Zhejiang, and 44 scattered in 11 other provincial administrative regions. The pooled prevalence of HTLV in blood donors in Fujian, Guangdong and Zhejiang provinces were 3.25/10 000(95%CI 1.91/10 000~4.58/10 000), 0.13/10 000(95%CI 0.09/10 000~0.17/10 000) and 0.33/10 000(95%CI 0.06/10 000~0.61/10 000), respectively. Subgroup analysis showed that the pooled prevalence of HTLV in Fujian blood donors decreased since December 2015. 【Conclusion】 Blood donors in mainland China present low HTLV prevalence, mainly distributed in three coastal provinces as Fujian, Guangdong and Zhejiang. The sample size tested increased greatly since HTLV screening for blood donors was popularized nationwide in blood stations in December 2015, the infection rate of HTLV in Fujian, however, did not increase as expected.

【Objective】 To analyze the related factors affecting first-time platelet donors to become regular donors in Guangzhou in 2018, so as to provide reference for the retention of platelet donors. 【Methods】 The data of 4 361 first-time platelet donors in Guangzhou in 2018, including age, gender, initial donation unit, time for the primary and secondary donation were collected. The relevant factors affecting first-time blood donors to become regular donors were analyzed by Logistic regression analysis. 【Results】 Among the influencing factors, first-time donors returned in the first month were more likely to become regular donors (5.569 times that of those did not return)(OR 95% CI: 4.748~6.533, P<0.05). First-time donors who aged 45~60, and initially donated 2 units were more likely to become regular donors than those aged 18~24(1.857 times) (OR 95% CI: 1.413~2.441, P<0.05), and those initially donated only one unit (1.695 time) (OR 95% CI: 1.486~1.933, P<0.05). 【Conclusion】 The returning in the first month, age and initial donation units predict long-term commitment for first-time donors. Encouraging first-time platelet donors to return within 1 month is more likely to convert them into regular blood donors.

【Objective】 To apply the spatial distribution analysis based on ArcGIS software, which has been applied widely in other fields, so as to analyze the intended locations for whole blood donation. 【Methods】 After a random stratified sampling was conducted among blood donors in the 17 donation sites during August 1st, 2021- July 30th, 2022, their intended blood donation locations were collected by an e-questionnaire. Addresses of donors′ intended donation locations were derived for GCJ-02 coordinates form and transformed by pandas module of Python to WGS84 coordinates, which further loaded to ArcGIS Arcmap module using Grouping Analysis for 17 median centers. The addresses of 17 blood donation sites in Guangzhou Blood Center were transformed to WGS84 coordinates and loaded to ArcGIS Arcmap module using the same methods for 3 ring buffer analysis. The criterion for judging whether the two were " matched" was whether the intended blood donation sites were covered by or adjacent to the 3 ring buffer zone of the existing blood donation sites. 【Results】 Of the 17 potential sites obtained from the spatial distribution analysis of 40 523 valid addresses of donors, 8 sites were covered or adjacent to the buffer of the existing donation sites, while the other 9 sites were far away from the existing donation sites. 【Conclusion】 By analyzing the spatial distribution of donors′ intended donation addresses, we can find out the service needs of donors for donating blood conveniently, which can provide basis for further blood donation service optimization.

【Objective】 To further determine the relationship between blood donor role cognition and blood donation behavior, then prepare of blood donor role identity scale(BDRIS). 【Methods】 The preparation of the scale was divided into five stages. It includes literature retrieval, expert interview, construction of the basic framework of the scale, scale test and statistical index test. The study focused on items selection, dimensions identification, measurement reliability, content and structural validity. 【Results】 The blood donor role identity scale consisted of 35 items. Exploratory factor analysis divided the items into six common factors, including role identity, role expectation, current situation of blood collection and supply, role conflict, external reward and blood donation experience. The α of scale was 0.840. 【Conclusion】 The reliability and validity of the scale meet the basic measurement requirements, and the framework of the blood donor role scale is basically formed. It can be used as one of the means to explore the ways and mechanisms of the role identity of blood donors in blood donation behavior.

【Objective】 To analyze the researches on blood donation service during 2001 to 2020, explore the development of blood donation service in China and discover the hot spots and weaknesses in current research, aimed to provide reference for future research. 【Methods】 The research team, institutions and hotspots of related literature from China National Knowledge Infrastructure (CNKI) were analyzed using CiteSpace. 【Results】 After analyzing the selected 969 relevant literature, it is concluded that there is few communication between the core author teams. The inter-agency research network is not mature enough, and the cooperation and communication between institutions need to be further strengthened. The high-frequency keywords were volunteer donors, satisfaction, blood donation response and humanized service. Quality control in the blood donation service process, maintenance and recruitment of regular blood donors, and the establishment and improvement of voluntary blood donation teams were research hot spots. 【Conclusion】 China has made fruitful achievements in the research of blood donation service after 20 years of development. The key point of future research is to strengthen the cooperation between different research teams, integrate the first-line practice of blood collection and supply, as well as explore the individualized and localized theory of blood donation service.

Recently the studies on mosquito genomics, transcriptomics and small RNAomics developed rapidly with the novel biotechnologies of the next generation sequencing techniques. The genome sequences of several important vector mosquitoes including Anopheles gambiae, Culex quinquefasciatus, and Aedes aegypti have been published. The genome sizes vary among the different species of mosquitoes and are consistent with the number of the repeat regions. The released genome sequences facilitate gene cloning and identification as for OBP, OR and dsx genes. Transcriptomics provides a useful tool for functional analyses of the mosquito genes, and using this technique, the molecular basis of mosquito blooding, gland proteins and diapauses have been explored. Studies on small RNAomics suggest important roles of miRNAs and piRNAs in ovary development, blood digestion, and immunity against virus infection. The studies on mosquito omics have generated a big data platform for investigation of vector biology and vector-transmitted disease prevention.

【Objective】 To investigate the changes of platelet, white blood cell, red blood cell counts, Hb and PCV before and after plateletpheresis donation. 【Methods】 10 444 platelet donors who donated platelet from Aug 1, 2019 to Jul 31, 2020 were selected as subjects, among which first-time donors were selected as control group and donors who donated twice or more as donation group. After matching gender and age using Propensity Score Matching method in the two groups, the changes of platelet, white blood cell, red blood cell counts, Hb and PCV of platelet blood donors were compared by t test. 【Results】 There were a slight increase in Plt (t=-6.651, P<0.05), a decrease in WBC (t=2.312, P<0.05), a decrease in hemoglobin(t=4.643, P<0.05), and no change in RBC (t=1.439, P>0.05) and in PCV (t=0.030, P>0.05) after plateletpheresis donation. 【Conclusion】 Plateletpheresis donation has an effect on some peripheral blood parameters, but within the normal reference range.