Induced pluripotent stem cells (iPSCs)are pluripotent stem cells obtained through transduction of specific transcription factors and reprogramming of human and animal somatic cells,Which are similar to embryonic stem cells.The iPSCs possesses characteristics of unlimited proliferation,self-reneWal and multi-differentiation.This arti-cle mainly summarized current research condition,application value and prospects of iPSCs in cardiovascular area, and its defect in application of current stage.

Objective To evaluate the effectivness of photodynamic therapy (PDT) with chloroaluminum sulfonated phthalocyanine (CASPc) in B16F10 melanomas in a rabbit model. Methods B16F10 murine melanoma tumor fragments were implanted transclerally into the subchoroidal space of 38 immunosuppressed New Zealand albino rabbits and examined with indirect ophthalmoscopy and ultrasonography. When the tumors ranged from 2.0~3.8 mm in height, 30 rabbits were treated by PDT, with intravenous injection of CASPc 5 mg/kg, and irradiation at the wavelength of 675 nm of an argon-pumped dye laser after 24 hours. Light dose ranged from 20 J/cm 2 to 70 J/cm 2. The other 8 animals were treated with light only or photosensitizer only. The animals were followed up for 6~8 weeks. Results The 30 tumor-bearing rabbits were by PDT (laser and CASPc) regressed in 21 animals after treatment. At light doses under 40 J/cm 2, tumor regrowth was observed in 9 animals after two weeks of treatment. In all of the 8 control animals, the tumor-bearing eyes were filled with tumor at the third week after implantation with laser doses of 70 J/cm 2. Conclusion The study suggest that CASPc PDT may be effective in the treatment of B16F10 choroidal melanomas.

Objective:To establish the quality standards for Zhichuan adhesive plasters;Methods:Herba ephedrae、Bergenin arolisiae、Flos caryophylli、Semen sinapis in Zhichuan adhesive plasters were identified by TLC. Ephedrine hydrachloride in Herba ephedrea was determined by RP HPLC.Results:These methods were simple and accurate.Conclusion: The methods can be used for the quality control of Zhichuan adhesive plasters.

Purpose To discuss the TCR gene rearrangements in the diagnosis of T-cell lymphomas. Methods Formalin-fixed and paraffin-embedded samples including 30 cases of T-cell lymphomas and 30 cases of reactive lymphoid hyperplasia were chosen for ex-tracting genomic DNA and PCR amplification using 56 BIOMED-2 primers. PCR products were analyzed by heteroduplex and polyacryl-amide gel electrophoresis. Results In all 30 cases of T-cell lymphomas, 25 cases (83. 3%) showed TCRβ gene monoclonal rear-rangements, 28 cases (93. 3%) of TCRγ gene monoclonal rearrangements, 4 cases (13. 3%) of TCRδ gene monoclonal rearrange-ments. 29 cases (96. 7%) with TCRβ+TCRγ+TCRδ gene monoclonal rearrangements were detected. but no clonal TCR gene rear-rangements were found in 30 cases of reactive lymphoid hyperplasia. Conclusions The detection of TCR gene rearrangements using BIOMED-2 primers is a useful assistant method for the diagnosis of T-cell lymphomas.

Objective :To study the effects of intercellular adhesion molecule-1 (ICAM-1) and human leukocyte antigen (HAL-DR) on the immunopathologic process of uveitis. Methods:Imn- munohistochemical techniques were applied to detect their expression in eyes of both the health (20 cases from eye bank) and patients with uveitis (54 cases with 54 eyes which included 33 ex- ogenous uveitis and 21 endogenous one). Results:Both the two ant igens were detectable in the choroidal and retinal tissues in eyes of uveitis while all the normal eyes showed negative expres- sion of ICAM-1 and negative or little expression of HLA-DR (P＜0. 01). However,there was no statistically significant difference between exogenous and endogenous types (P＞0. 05). Conclu- sion: Both ICAM-1 and HLA-DR may be responsible for cell recognition and binding in the in- flarnmatory tissues. The co-expression of ICAM-1 and HAL-DR showed that these two factors might play an important role in the immunologic damage of the choroid and retina in uveitis.

An ultrastructural analysis of distal nephron has been investigated using morphometric method for rats with hyperthyroidism and hypothyroidism. The cells analysed were distal convoluted tubule cells (DCT cells), distal straight tubule cells (DST cells), intercalated cells (I cells) and principal cells (P cells) of collecting duct. The cells were sampled from superficial cortex. The results revealed that in DCT, DST and P cells the profile area of cells, the surface density of plasma membrane (Sv), the boundary length of cell surface [B(A)], the volume density (Vv) of mitochondria, and the length of microvilli were significantly decreased in the rat with hypothyroidism. However, in the rat with hyperthyroidism there were an obvious increase of profile area of cell and Vv of mitochondria in I cells. The results suggest that thyroid hormone is an important factor for maintaining the normal cellular structure in the distal nephron. The morphometry shows that the changes of DCT, DST and P cells are distinct in the rat with hypothyroidism, and the ultrastructural changes of I cell are obvious in the rat with hyperthyroidism.

Objective To evaluate quantitatively the standing balance function of patients with lower limb prosthesis. Methods Twenty-two patients were divided into three groups according their conditions: group 1 consisted of patients with one leg amputated, group 2 consisted of patients with one amputated at high level, group 3 consisted of patients with both thighs amputated, and twenty-one normal subjects served as control. Parameters including the sway magnitude of gravity center, circumference area, drift in X vector, etc., were recorded and analyzed. Results The data obtained revealed that the sway magnitude, circumference area were increased, in all the cases, especially in those with lower limb prosthesis at both sides. Compared to the control group, the difference was significant( P

ObjectiveTo explore the effect of partial body-weight supported treadmill training(BWSTT) on gross motor function measure, walking ability and efficiency, and functional ambulation categories in children with cerebral palsy(CP).Methods35 children with cerebral palsy were assigned into 3 groups, BWSTT group(BWSTT with PT, n=11), TT group(treadmill training with PT, n=12), and PT group (n=12). Assessments were performed before treatment and 8 weeks and 12 weeks after treatment respectively. The evaluation items included the D & E dimensions of the gross motor function measures (GMFM), walking ability(WA), walking efficiency(WE)and Functional ambulation categories (FAC).ResultsNo statistically significant differences were found in age, gender, body height, as well as clinical assessments (GMFM, WA, WE and FAC) before treatment between 3 groups(P>0.05). There was statistically significant difference in each group 12 weeks after treatment, and BWSTT group showed statistical significant difference in GMFM, WA, WE and FAC.ConclusionBWSTT could significantly improve the walking ability and walking efficiency, and also the standing and walking ability of children with CP.