miR-205 is differentially expressed in tumor tissues and is closely related to tumorigenesis and tumor metastasis.miR-205 can regulate the biological behaviors of tumor cells such as cell proliferation,differentiation,in situ invasion and distant metastasis by post-transcriptional regulation through binding to targeted genes.Additionally,further research of miR-205 may be helpful for tumor diagnosis,targeted therapy and prognosis.

Objective To observe the effects of differentially expressed peroxisome proliferatoractivated receptor γ (PPAR γ) on cell migration,invasion and proliferation of endometrial cancer cells.Methods Two endometrial cancer cell lines ECC-1 (ER positive) and KLE (ER negative) cells were used in this study.To up or down regulate PPARγ expression,the transient transfection by using PPARγ expression vector (PPARγ expression vector group) and PPARγ small interference RNA (PPARγ siRNA group) were done.The negative control groups were cells transfected by nonsence sequence siRNA (siRNA non sence sequence group) or empty vector (empty vector group).At the same time,cells only added with liposome were used as blank control group.Then,quantitative real time (RT)-PCR and western blot were used to detect PPARγexpression both in mRNA and protein levels.To assess the expression levels of Wnt signaling pathway,western blot was performed to analysis protein levels of β-catenin and C-myc.The effects on cell migration,invasion and proliferation using in vitro transwell migration,invasion assays and cell counting kit-8 (CCK-8) assay were further be examined.Results After transfection for 48 hours,quantitative RT-PCR and western blot showed that PPARγmRNA (5.18 ± 0.99,4.54 ± 0.89) and protein (1.45 ± 0.12,1.30 ± 0.13) expression levels significantly increased and the protein levels of β-catenin (0.44 ± 0.06,0.46 ± 0.04) and C-myc (0.42 ± 0.08,0.30 ± 0.11) decreased in PPAR γ expression vector group,while in PPARγ siRNA group,PPARγ mRNA (0.48 ± 0.08,0.53 ± 0.11) and protein (0.41 ±0.04,0.49 ±0.05) expression levels decreased and the protein levels of 3-catenin (1.18 ±0.12,0.89 ±0.07) and C-myc(0.91 ±0.08,0.77 ±0.12) increased significantly compared with control groups (all P ＜ 0.05).In vitro migration and invasion assay indicated that the migratory and invasive cell numbers of PPARγ expression vector group (ECC-1:129 ± 9,63 ± 12 ; KLE:119 ± 9,68 ± 16) were significantly decreased,while the migratory and invasive cell numbers of were PPARγ siRNA group (ECC-1:201 ± 14,142 ±9 ; KLE:170 ± 11,138 ± 7) increased significantly compared with those in control groups(all P ＜ 0.05).CCK-8 assay showed that A values (0.66 ±0.14,0.78 ±0.06) in PPARγexpression vector group were lower than those in control groups,and in PPARγ siRNA group,A values (1.42 ± 0.16,1.23 ± 0.04) were higher than those in control groups,and there were statistically significant difference among them (all P ＜ 0.05).Conclusion Up-regulated PPARγ gene expression could inhibit endometrial cancer cell migration,invasion and proliferation abilities,and down-regulated PPARγ gene expression could promote endometrial cancer cell migration,invasion and proliferation abilities.

Objective:To investigate the expressions of peroxisome proliferator-activated receptor gamma (PPARγ), estrogen re-ceptor alpha (ERα), and estrogen receptor beta (ERβ) in endometrial carcinoma and to analyze their correlations and clinical signifi-cance. Methods:Immunohistochemical assay and Western blot were used to detect the expressions of PPARγ, ERα, and ERβin normal endometrial tissues and well-differentiated, moderately differentiated, and poorly differentiated endometrial carcinomas. Results:PPARγexpression was significantly lower in endometrial carcinoma than in the normal endometrium and was intimately associated with cli-ni-copathologic variables. ERαexpression gradually decreased in moderately and poorly differenti-ated endometrial carcinomas. How-ever, no significant differences were found between the normal endometrium and well-differentiated endometrial carcinoma. ERβex-pression only decreased in the poorly differentiated endometrial carcinoma. No significant association was observed between ERβand clinicopathologic variables. Pearson correlation analysis showed a significant positive cor-relation between the expressions of PPARγand ERα. No correlations were observed between the expressions of ERαand ERβand between that of ERβand PPARγ. Conclusion:The expression lev-els of PPARγand ERαwere significantly associated with the clinicopathologic stage of endometrial carcinoma, and have essential functions in endometrial tumorigenesis and tumor progression.

OBJECTIVE:To study the protective effect of shuanghuanglian sterilized powder for injection on hepatic in?jury of model mouse induced by human cytomegalovirus(HCMV)infection.METHODS:The mice were randomized into nor?mal control group,infection model group,ganciclovir group and shuanghuanglian sterilized powder for injection group(high,middle and low dosage),all of which except the normal control were vaccinated with HCMV5d to establish the hepatic injury model,each group was administered with different drugs;10days later,the enzyme activity of ALT and AST in the eye sockets blood of each group was determined;The mice were put to death by cervical vertebra dislocation and the necrosis degree of livers from the dead mice was evaluated and the pathological observation was conducted.RESULTS:The activity of ALT and AST were normal in the normal control,and no significant changes were found in the livers of this group;Compared with the normal control,the plasma ALT and AST levels have been increased significantly and there was a significant pathological hepatic injury in the infection model group;Compared with the infection model group,significant decreases were found in the activity of ALT and AST in the high and middle dosage groups of shuanghuanglian sterilized powder for injection,and there were obvious alleviated pathologic hepatic injury while no significant differences were found in the plasma ALT and AST ac?tivity in the low dosage group;Compared with the infection model group,the ALT and AST activity of the ganciclovir group were lowed significantly;Compared with the ganciclovir group,no significant differences were found in the ALT and AST ac?tivity in the high and middle dosage groups of shuanghuanglian sterilized powder for injection.CONCLUSION:Shuanghuan?glian sterilized powder for injection does have protective effect on hepatic injury of model mouse induced by HCMV.

Aim To investigate the effect of Shuanghuanglian on the the load of hepatic HCMV DNA in mice infected with HCMV.Method The model of mice hepatitis infected with HCMV was prepared. 10 days after treated with Shuanghuanlian,the hepatic HCMV DNA was detected qualitatively by polymerase chain reaction;the level of alanine aminotransferase (ALT) and aspartate aminotransferase(AST) in serum were assayed by spectrophotometer;and hepatic pathology was observed with HE staining.Results HCMV DNA load in infected model group was 3875.4760 copies/mg,while in high and middling dosage shuanghuanglian therapy group it was 8.3261 copies/mg、9.1476 copies/mg respectively;It decreased significantly compared with the former(P0.05);low dosage of shuanghuanglian(HCMV DNA load was 3812.8980 copies/mg) had no notable difference compared with infected model group;serum transaminase (ALT,AST) levels significantly elevated in infected model group; serum transaminase level decreased sharply and liver histological pathology was lighten after treated with high and middling dosage of Shuanghuanglian(P

OBJECTIVETo evaluate the therapeutic effect of cervical conization through hysteroscopy in the treatment of cervical intraepithelial neoplasia (CIN) III.

METHODSSeventy-four patients with CIN III underwent cervical conization through hysteroscopy (TCRC group), and 65 received cold knife conization (CKC group). The operating time, volume of blood loss, concordance rate with pathology, recurrence rate, rate of cervix adhesion and pregnancy rate were compared between the two groups.

RESULTSThe operating time, mean blood loss, cure rate, and recurrence rate were 15.1∓3.2 min, 12.5∓1.8 ml, 94.6%, and 5.4% in TCRC group, respectively, as compared with those of 25.8∓3.8 min, 21.6∓2.4 ml, 81.5%, and 18.5% in CKC group, all showing significant differences between the two groups (P<0.05).

CONCLUSIONCompared with CKC, TCRC has such advantages as less blood loss, shorter operating time, more accurate lesion localization, fewer complications, higher cure rate, and lower recurrence rate without significant adverse effect on pregnancy.

Adult ; Cervical Intraepithelial Neoplasia ; pathology ; surgery ; Cervix Uteri ; surgery ; Female ; Humans ; Hysterectomy ; methods ; Hysteroscopy ; Middle Aged ; Uterine Cervical Neoplasms ; pathology ; surgery

OBJECTIVE To detect potential mutations of the EXT1 and EXT2 genes in a pedigree affected with hereditary multiple exostosis (HME). METHODS For a four-generation family with 7 affected individuals from 17 family members,genomic DNA was extracted from peripheral venous blood samples. All exons of the EXT1 and EXT2 genes were screened for potential mutation by PCR and Sanger sequencing. RESULTS A novel heterozygous frameshift mutation c.1202delT (p.I401Tfs*2)was found in exon 4 of the EXT1 gene in the proband and the other 6 affected individuals. The same mutation was not detected among the healthy members from the family. The mutation has given rise a truncated EXT1 protein with loss of 345 amino acids. CONCLUSION A novel frameshift mutation of the EXT1 gene has been identified in a pedigree affected with HME, which has enriched the mutational spectrum of the EXT1 gene and may facilitate genetic counseling and prenatal diagnosis for the family.

OBJECTIVETo detect potential mutation of the WAS gene in a Chinese family affected with Wiskott-Aldrich syndrome.

METHODSPeripheral blood samples were collected from the proband and his family members. All exons and flanking regions of the WAS gene were subjected to PCR amplification - Sanger sequencing as well as restriction endonuclease analysis. Plasma level of B-cell activating factor (BAFF) was also determined for all family members.

RESULTSA hemizygous mutation (c.257G>A) of the WAS gene was identified in all patients from the family, for which the patient's mother was heterozygous. The same mutation was not found among healthy members of the family. Compared with unaffected members, all patients had a higher level of BAFF.

CONCLUSIONThe c.257G>A mutation of the WAS gene probably underlies the Wiskott-Aldrich syndrome in this family.

B-Cell Activating Factor ; blood ; Child, Preschool ; Heterozygote ; Humans ; Male ; Mutation ; Wiskott-Aldrich Syndrome ; genetics ; Wiskott-Aldrich Syndrome Protein ; genetics

OBJECTIVE: To explore the genetic basis for a fetus suspected for congenital nephrotic syndrome of Finland (CNF). METHODS: Genomic DNA was extracted from peripheral and umbilical cord blood samples derived from both parents and the fetus. Potential variants were detected by using next-generation sequencing. Suspected variants were confirmed by Sanger sequencing. RESULTS: The fetus was found to carry compound heterozygous variants c.1440+1G>A and c.925G>T of the NPHS1 gene, which were respectively inherited from its mother and father. CONCLUSION Identification of the compound heterozygous NPHS1 variants has enabled diagnosis of CNF in the fetus and genetic counseling for the affected family.
Female ; Fetus ; Finland ; Heterozygote ; Humans ; Membrane Proteins ; genetics ; Nephrotic Syndrome ; congenital ; diagnosis ; Pregnancy ; Prenatal Diagnosis

OBJECTIVE: To explore the genetic basis for a Chinese pedigree affected with dyschromatosis symmetrica hereditaria (DSH). METHODS: PCR and Sanger sequencing were carried out for the proband, and suspected variant was validated by Sanger sequencing in the pedigree. RESULTS: The proband was found to harbor a novel variant of c.1352delA (p.N451Mfs*13) of the ADAR (NM_001111) gene. The same variant was found in her affected mother and sister, but not in her unaffected father, uncle, and 100 healthy individual. CONCLUSION The novel variant of the ADAR gene probably underlay the pathogenesis of DSH in this pedigree.
Adenosine Deaminase/genetics* ; China ; Female ; Humans ; Mutation ; Pedigree ; Pigmentation Disorders/congenital* ; RNA-Binding Proteins/genetics*