Objective To observe the comprehensive effect of different drugs for ectopic endometrium after laparoscopic surgery.Methods According to random number,75 intrauterine membrane patients with endometriosis treated by laparoscopy were divided into control group(25 cases),mifepristone group(25 cases)and gestrinone group (25 cases).The patients of the control group were not taken medication,the patients of mifepristone group in postop-erative clothing were treated with mifepristone,the patients of gestrinone groups in postoperative plus gestrinone treat-ment.All of the patients were taken 6 months treatment,therapy,pregnancy rates,and adverse reactions of three groups were observed.Results The total effective rate of the control group was 76.0%,which of the mifepristone group was 96.0%,the difference between the two groups was statistically significant (χ2 =4.15,P <0.05),which of the gestri-none group was 92.0%,and the difference between the two groups was statistically significant(χ2 =2.38,P <0.05). The incidence rate of side effects in mifepristone group was 12.0%,which was lower than 36.0% of gestrinone group,the difference was statistically significant (χ2 =8.39,P <0.05 ).The patients combined infertility were 17 cases,after the treatment for pregnancy 8 cases,the cumulative pregnancy rate of mifepristone group was 66.7%, which was higher than 20.0% of gestrinone group,and the difference was significant (χ2 =4.15,P <0.05).Conclusion Simple using abdominal mirror operation treatment uterine within film different bit syndrome patients of effect not too ideal,but operation through taking meters non -division ketone or pregnancy three ketone can obviously improve treat-ment has efficiency,reduced recurrence rate,while can increased pregnancy rate and reduced patients of bad reaction occurred rate,is a security efficient of treatment programme,worth promotion in the clinical treatment.

Objective To investigate expressions of microRNA-21 (miR-21),PTEN,NF-κB,and caspase-9 in colon carcinoma and their possible mechanisms in progression of tumor.Methods Expressions of above-mentioned indexes and percentage of apoptotic cells in colon carcinoma,colon adenoma,and normal tissue specimen were detected.Results Expressions of miR-21,PTEN,N F-κB,and caspase9 in normal tissue were 0.39 ±0.02,50％,25％,and 50％.Expressions of them in colon adenoma were 0.62 ±0.06,50％,35％,and 55％,and those in colon carcinoma were 0.88 ±0.04,30％,75％,and 20％.Percentage of apoptotic cells were (24.64 ± 7.66) ％,(15.86 ± 1.44) ％,and (6.20 ± 2.57) ％,respectively.The above P-values were all less than 0.05.Expression of miR-21 was positively correlated with TNM stage,depth of infiltration,differentiated level and expression of NF-κB while inversely correlated with PTEN and caspase-9 expressions and percentage of apoptotic cells.The r-values were 0.647,0.297,0.259,0.519,-0.299,-0.388,and-0.931,respectively.Conclusions Hyperexpression of miR-21 participated in progression of colon carcinoma probably through down-regulating expressions of PTEN and caspase-9 and up-regulating expression of NF-κB and then suppressing cell apoptosis.

Objective To investigate the correlation between behavioural and psychological symptoms and cerebral white matter lesions (WMLs) in patients with Alzheimer's disease (AD). Methods Sixty patients with AD aged more than 60 years (AD group) and 40 age-, sex-matched subjects with normal cognitive function (control group) were recruited. data of cerebral CT were analyzed blindly. The sum of WMLs in 8 regions, such as bilateral frontal region, parieto-occipital region, temporal region, and subtentorial region, etc were used as the total score of WMLs, and the cerebrovascular lesions revealed on CT were detected. The AD group was assessed with the Neuropsychiatric Inventory (NPI). Results There were significant differences in total WML score (1. 450 ±2. 310 vs. 9. 640 ± 1. 566, P =0. 000), left frontal WMLs (0. 340 ± 0. 340 vs. 1. 720 ± 0. 248, P = 0. 001), fight frontal WMLs (0. 310 ±0. 560 vs. 1. 680 ±0. 312, P =0. 001), left parieto-occipital WMLs (0. 240 ±0. 360 vs. 1. 550 ±0. 244, P =0. 012), right parieto-oecipital WMLs (0. 140 ±0. 230 vs. 1. 450 ± 0. 231, P = 0. 006) and cerebrovascular lesions (0. 120 ± 0. 330 vs. 2. 230 ± 0. 378, P = 0. 002) hetwsen the AD group and the control group. Spearman correlation analysis showed that NPI was correlated with the total WML score (rs =0. 487, P = 0. 016), left frontal WMLs (rs =0. 490, P = 0. 014), right frontal WMLs (rs = 0. 502, P = 0. 014), left parieto-occipital WMLs (rs =0. 507, P =0. 012) and right parieto-occipital WMLs (rs =0. 521, P = 0. 014), and it was not correlated with the cerebrovascular lesions (rs = 0. 132, P = 0. 590).Conclusions WMLs and cerebrovascular lesions are essential in the onset of AD and disease development. WMLs are closely correlated with the behavioural and psychological symptoms in patients with AD.

Objective To explore the expressions and the roll of p63mRNA and murine double minute 2(MDM-2) protein in esophageal squamous cell cancer and the prognosis of the cancer.Methods The expressions of p63mRNA and MDM-2 protein in 38 cases of esophageal squamous cell carcinoma tissues and the corresponding esophageal normal tissues were detected using reverse transcriptase polymerase chain reaction(RT-PCR) and immunohistochemistry. Results The positive rates of deltaNp63 mRNA in all malignant and normal tissues were 63.5% and 52.63% respectively,without significant difference.The expression of the DeltaNp63 mRNA was significantly higher in low differentiated group than in well and moderate differentiated group,without significant difference in age,gender,lymph node metastasis and clinical stage.Only 1 of 38 cases of esophageal squamous cell carcinoma tissues presented TAp63 mRNA expression and all esophageal normal tissues had no TAp63 mRNA expression.The positive rates of MDM-2 protein in esophageal squamous cell carcinoma tissues and normal tissues were 65.79% and 5% respectively,with significant difference.The expression level of the MDM-2 protein was higher significantly in low differentiated group than in well and moderate differentiated group,with significant difference in clinical stage and without significant difference in age,gender and lymph node metastasis.The DeltaNp63 mRNA expression in the esophageal squamous cell carcinoma tissues was positively related to MDM-2 protein expression.Conclusion The major isotype expressed in the esophageal cancer and epithelia is DeltaNp63,which may play an important role in the carcinogenesis,development and prognosis of the esophageal squamous cell carcinoma.TAp63 is not the major isotype expressed in the esophageal cancer and epithelia,and not related to the carcinogenesis of esophageal squamous cell carcinoma.High expression of MDM-2 protein is associated with the carcinogenesis of esophageal squamous cell cancer and related to the prognosis of esophageal squamous cell carcinoma.DeltaNp63 regulates the cell cycle and apoptosis through the pathway of MDM2 and causes the carcinogenesis of esophageal squamous cell cancer.

0.05) were seen among the cases given autohemotransfusion including hemodilution and the use of retrieved blood comparing with the allogeneic transfusion. Therefore, hemodilution and retrieving blood for retransfusion are safe and reliable ways to be adopted in orthopaedic surgery.

Objective To compare the effect of myomectomy and uterine artery embolization in the treatment of uterine fibroids .Methods 158 patients who were pathologically diagnosed uterine fibroids were randomly divided into the uterine artery embolization group and myomectomy group ,79 cases in each group .The blood loss 3 days after operation,pelvic pain and pressure symptoms ,hospitalization time,follow-up and time of normal activity were recorded and compared.Results The pelvic pain,pressure symptoms,hospitalization time,cure rate,incidence rate of compli-cations and recurrence rate of the two groups had no statistically significant differences ( t=1.21,0.97,1.06,χ2 =0.98,1.21,1.32,all P>0.05).The postoperative bleeding score ,time of return to normal activity in the uterine artery embolization group were (5.8 ±0.5) points,(9.8 ±1.8) d,which were better than those of the myomectomy group[(4.9 ±0.4)points,(25.9 ±5.1)d](t=3.76,5.98,all P<0.05).Conclusion The effect of the myomec-tomy and uterine artery embolization treatment for uterine leiomyoma is similar ,and both have high efficiency and safe-ty,but the uterine artery embolization treatment has less impact on the body of patients .

Objective To investigate the effect of indomethacin on the protein expression profile of colorectal cancer cell line HCT116 xenografts in nude mice, and to screen the indomethacin anticancer-related proteins. Methods The nude mice with colorectal cancer cell line HCT116 xenograft were treated with or without indomethacin, and then the total proteins extracted from xenografts were separated and visualized by immobilized pH gradient two-dimensional gel (2-DE) electrophoresis. The differential expression proteins between the experimental and control groups were analyzed using PDQuest image analysis software, and identified by peptide mass fingerprinting based on matrix-assisted laser desorption ionization time of flight mass spectrometry and database searching. Results The 2-DE patterns with high resolution and reproducibility were obtained, displayed about 1100 protein spots in each gel, and the matching rate in the two groups was 96. 0% and 93. 6% , respectively. Thirty-one differentially expressed protein spots were found between the two groups, of which 25 spots decreased and 6 increased in the expression levels in the experimental group than in the control group. Eighteen peptide mass fingerprinting maps were obtained, and twelve proteins including galectin-1, annexin and trancription factor, were primarily identified after database searching, most of which were involved in tumor cell proliferation, invasion, apoptosis and immunity. Conclusions Indomethacin exerts its inhibiting effects on colorectal cancer through regulating multiple proteins expression directly or indirectly. The data will be helpful to elucidate the mechanism inhibiting cancer by indomethacin, and to find new targets of chemotherapeutic drugs.

Using inverted microscopy and circuit TV, the morphological type, diameter of arteriovenous anastomoses (AVAs) in 11 rabbit's ear chambers were observed. According the joining forms with arterioles and venules in 68 AVAs, there were 4 types: type Ⅰ 58.8％, type Ⅱ 19.4％, type Ⅲ 11.1％, type ⅣV 10.7％. The inner diameters of the AVAs when dialated, measured 36.6?1.67?m at their arterial ends, 16+0.6?m at their narrow points, 66?4?m at their venous ends.This study showed that dilation of AVAs in the rabbit's ear is a graded response which correlates with the rise in intravertebral canal temperature. Some AVAs have multiple venous segments which vary in diameters. It was observed that the behaviour were different after intravertebral canal warming.

Objective It has been known that NSAIDs can inhibit the growth of tumors through cyclooxygenase, but it is unknown that whether there are other mechanisms involved. It is essential to detect the effect of indomethacin for proliferation and apoptosis in the HCT116 and explore its anti tumor mechanism. Methods Human colon adenocarcinoma cell line HCT116 was treated with different concentration of indomethacin for 24 h and CDK 2,CDK 4, Bcl 2, Bax and p21 WAF1/CIP1 protein were detected by Western blot. Results Indomethacin down regulated the expression of CDK 2, CDK 4, Bcl 2 and up regulated the expression of p21 WAF1/CIP1 , however, the expression of Bax remained unchanged. Conclusions Inhibiting proliferation and inducing apoptosis contribute to the anti tumor activity of indomethacin by down regulating the expression of CDK 2, CDK 4, Bcl 2 and up regulating the expression of p21 WAF1/CIP1 .