OBJECTIVE: To construct dopamine D1 receptor (DRD1) expression interference vectors to study the role of DRD1 in nerve cells and lay a foundation for drug development in anti-convulsion. METHODS: Based on DRD1 gene sequence in GenBank, 10 interfere vectors of DRD1 were designed. Liposomal was used to transfect NG-108-15 and the transfect effect was assayed by GFP. With realtime PCR and Western blot, the DRD1 expression was detected. RESULTS: The 10 constructed interfere vectors transfected into NG-108-15 cells by liposomal method and inhibited DRD1 mRNA and protein expression. DRD1 mRNA expression in NG-108-15 cells transfected with pGPU6-GFP-Neo-si-DRD1-5 was the lowest whereas DRD1 protein expression in NG-108-15 cells transfected with pGPU6-GFP-Neo-si-DRD1-1, -2, -6, -7 was the lowest. CONCLUSION DRD1 expression interference vector is successfully constructed.
Animals ; Cell Line, Tumor ; Genetic Vectors ; Glioma ; pathology ; Hybrid Cells ; Liposomes ; metabolism ; Mice ; Neuroblastoma ; pathology ; RNA Interference ; RNA, Messenger ; genetics ; metabolism ; RNA, Small Interfering ; genetics ; Receptors, Dopamine D1 ; genetics ; metabolism ; Transfection

The diagnosis,treatment and pregnancy outcome of a case of pregnancy associated Sj(o)gren's syndrome characterized by hyperglobulinemia were reported here.The patient who had a history of repeated purpura and anemia was admitted at 26+4 weeks of gestation with premature rupture of membranes.Multiple prenatal examinations showed elevated globulin without any other symptoms such as dry mouth,dry eyes,etc.No relevant tests was offered to confirm the diagnosis before or during pregnancy.After admission,she was diagnosed as Sj(o)gren's syndrome through immunoelectrophoresis and autoimmune antibody test,and hydroxychloroquine was administered.The patient gave birth to a 870 g newborn baby at 27 weeks of gestation.The premature infant was transferred to the Neonatology Department and later discharged after 2.5 months of treatment.A 3-month postpartum follow-up revealed that the patient still suffered from hyperglobulinemia,abnormal erythrocyte sedimentation rate and rheumatoid factor level,therefore she continued oral hydroxychloroquine treatment and was regularly followed up.Clinicians should be alert to the possibility of Sj(o)gren's syndrome in gravidas with hyperglobulinemia.