Objective To investigate if the effects of integated traditional Chinese and westetrt medicine treat ing diabetic osteoporesis.Methods 120 patients aging from 50 to 70 years were randomly recruited into two groups according to theirgender,namely DN1 and DN2.DN1 groupwastreatedwitll vitamin D3 and DN2 groupwastreatedwitll vitaminD3 plus Tanggukang capsule.Results X-ray showed that the bone density in the DN2 group WaS higher than DNI group,and the difference was significant(P＜0.05).Conclusion The integrated traditional Chinese and western medicine has good effects in treating diabetic osteoporosis.

To investigate the chemical constituents of Psidium Guajava L, the EtOH/H2O extract of the fresh leaves was subjected to various chromatography. Five constituents with galloyl moiety were isolated and elucidated as 1-O-(1, 2-propanediol)-6-O-galloyl-beta-D-glucopyranoside (1), gallic acid (2), ellagic acid (3), ellagic acid-4-O-beta-D-glucopyranoside (4) and quercetin-3-O-(6"-galloyl) beta-D-galactopyranoside (5) by spectroscopic methods, including 2D NMR and HR-ESI-MS spectrometry as well as by comparison with published data. Compounds 4 and 5 were obtained from P. guajava for the first time, and compound 1 is a new polyhydroxyl compound.

Cytokine-induced killer (CIK) cells therapy plays an important role in cancer adjuvant treatment.Researches show that the number of peripheral blood immunocytes will change if patients with cancer accept the treatment of CIK cells.The changes of T cell subsets,regulatory T cells are relatively obvious,which may be one of the standards that can evaluate the curative effect of CIK cells.

To study the chemical constituents of Senecio nemorensis.,12 known compounds were isolated and identified,which were niacinamide(Ⅰ),vanillin(Ⅱ),syringic acid(Ⅲ),syringalddehyde(Ⅳ),3-acetyl-4-hydroxy-benzoic acid(Ⅴ),4,4-dimethyl-1,7-heptanedioic acid(Ⅵ),1H-indole-3-carboxaldehyde(Ⅶ),ethyl caffeate(Ⅷ),1-0-(E)-p-methoxycinnamoyl-β-D-glucopyranoside(Ⅸ),(6S,7E)-6-hydroxy-4,7-megastigmadien-3,9-dione(Ⅹ),Annuionone D(Ⅺ),and(1 'S,6'R)-abscisic acid(Ⅻ),respectively.

Poly ( aniline_luminol ) composite nanowires were synthesized by chemical oxidation using ammonium peroxydisulfate. In contrast to the maximum fluorescence emitting wavelength of luminol at 425 nm, the maximum fluorescence emitting wavelength of the polymeric luminol in the composite nanowires was red shifted to 465 nm obviously. The poly ( aniline_luminol) composite nanowires were modified on graphite electrode surface by drop coating, forming a stable poly ( aniline_luminol ) composite nanowires film. The composite nanowires film modified electrode presented favorable electrochemiluminescence ( ECL ) performances, and the ECL response could be enhanced by hydrogen peroxide. Under the optimized experimental conditions, the modified electrode provided a linear range of 5. 0×10-9-1. 0×10-5 mol/L for the detection of hydrogen peroxide with a detection limit of 2 ×10-9 mol/L.

Objective To produce the recombinant NS3 protease of the hepatitis C virus in insect cells. Methods The gene of HCV serine proteinase domain which encodes 181 amino acids was inserted into pFastBacHTc and the recombinant plasmid pFBCNS3N was transformed into DH10Bac competent cells for transposition. After the recombinant bacmid had been determined to be correct by both blue-white colonies and PCR analysis, the isolated bacmid DNAs were transfected into Sf9 insect cells. The bacmid DNA was verified to replicate in insect cells and package into baculovirus particles via PCR and electronic microscope analysis. The insect cells infected by recombinant baculovirus were determined by SDS-PAGE and Western-blot assays. The recombinant protein was soluted in N-Lauryl Sarcosine Sodium (NLS) and purifed by metal-chelated-affinity chromatography, then the enzymatic activity was measured with the protein substrate NS5ab and the antigenicity of recombinant protease was determined by enzyme-linked immunoabsorbent assay. Results The HCV NS3 protease domain was expressed in insect cells at high level and it was partially resolved in NLS;Totally 0.2 mg recombinant serine proteinase domain with high purity was obtained by metal-chelated-affinity chromatography from 5?10 7 cells and both enzymatic activity and antigenicity of the protein were proven to be good. Conclusion The recombinant HCV NS3 protease expressed in insect cells is a membrane-binding protein with good enzymatic activity and antigenicity.

Objective To discuss the content determination method ofα-asarone in Dianxianqing Granules. Methods Kromasil C18 column (250 mm×4.6 mm, 5 μm) was used, with methanol and water as mobile phase of gradient elution. The flow rate was 1.0 mL/min, and the detection wavelength was 257 nm. The column temperature was 25 ℃. Results The sample solution was prepared by reflux extracted with 70% ethanol for 20 min. α-asarone had a good linearity in the ranges of 1.24×10-3-7.44×10-3μg (r=0.999 5). The method was quite good in precision, stability and repeatability, and the average recovery ofα-asarone was 98.14%, with corresponding RSD of 2.5%. Conclusion The experiment provides an accurate and fast analytical method for α-asarone determination in compound preparation of traditional Chinese medicine.

Objective To investigate the related factors of phlebitis caused by PICC and support effective preventive nursing measures. Methods 163 patients diagnosed as malignant tumor who first used peripherally inserted central catheters (PICC) were recruited. Patients were observed for 3 months. According to the diagnostic criterion of infection, 34 patients with phlebitis were divided into the phlebitis group and others were divided into the control group. All patients were investigated for general information survey :sex, age, tumor type, allergic history,location of inserted vein, one-time success rate,activity after catheter, elbow bending,phlebitis occurred time, grade and type. Laboratory markers:WBC,neutrophils,immune parameters, fasting plasma glucose. Trait anxiety inventory (T-AI)was used for assessment of emotional state. Case-control study between groups was carried out. Results The results of univariate analysis showed that location of inserted vein,times of inserting vein,activity of inserted limb,elbow bending,blood sugar level and emotion were associated with phlebitis. Sex, age, tumor type, allergic history, immune function,WBC,neutrophils were not involved with phlebitis. The results of multivariate logistic regression analysis showed that PICC through basilica vein was the protective factor. Multiple insertions, more activity of inserted limb,durative anxious emotion were the risk factors of phlebitis. Conclusions Different location of vein,times of inserting vein,activity of inserted limb,elbow bending,high blood glucose level and persistent anxious emotion are the risk factors of phlebitis. Taking effective preventive measures before,during, and after PICC can reduce the incidence of phlebitis.

Objective To establish an ultra-high performance liquid chromatographic fingerprinting analysis method for the hydrophilic component of salvianolic acids and the lipophilic component of tanshinones in Radix Salviae Miltiorrhizae(RSM),and to supply a rapid and scientific method for quality control of RSM.Methods The chromatographic conditions were as follows: Acquity C18 BEH(2.1mm?100 mm,1.7 ?m)chromatographic column at 30℃,gradient elution with acetonitrile(A) and 0.4% formic acid(B)(90%~50% A for 0~10 min,75% A for 15 min),the flow rate being 0.5 mL?min-1 and UV detection wavelength set at 280nm.Results The chromatograms of the hydrophilic component and the lipophilic component were obtained within 15min,peak volume being 85.There were 20 peaks separated completely and identified by using HPLC-diode array detection-electrospray ionization-mass spectrometry.Conclusion The developed method is proved practicable,rapid and reliable for quality control of RSM and its preparations.

[Objective] To observe the effect of Anxin Granules (mainly composed of Radix Ginseng, Ramulus Cinnamomi, Pericarpium Trichosanthis, Hinido, Poria, etc.) in improving the symptoms of angina pectoris (AP) and acute inflammatory reaction in coronary heart disease (CHD) patients with AP. [Methods] Eighty senile CHD patients with AP of blood-stasis type were allocated to groups A and B with random number table. Group A was treated with Anxin Granules 3.5 g, tid combined with routine drug treatment and group B with routine drug treatment only. Four weeks constituted one treatment course. The changes of symptoms, electrocardiogram (EGG) and serum hypersensitive C-reactive protein (Hs-CRP) level were observed before and after treatment. [Results] In relieving symptoms and decreasing Hs-CRP level, group A was better than group B ( P 0.05) . Serum Hs-CRP level decreased in groups A and B after treatment as compared with that before treatment (P