Objective To observe the protective effect of Astragalus hoangtchy preconditioning on myocardial ischemia reperfusion injury in rabbits and structural and functional changes of mitochondria so as to provide prevention evidence of Astragalus hoangtchy being taken into clinical treatment for myocardial ischemia reperfusion injury.Methods Thirty-two healthy male rabbits were divided randomly into four groups: sham-operation group(Group A),ischemic reperfusion group(Group B),astragalus hoangtchy preconditioning group(Group C) and 5-HD plus Astragalus hoangtchy preconditioning group(Group D),with eight rabbits in each.All rabbits in the four groups were killed 2h after reperfusion.We examined Ca2+ concentration,MDA content,and SOD activity of myocardial mitochondria and observed ultrastructural changes of myocardium under electron microscope.Results SOD activity of myocardial mitochondria in Group C was significantly higher than that in Group B(P

The Outline-style homework is introduced, which is one of the characteristic diagnostic methods of TCM psychology. The outline-style homework contents include①the painful, sad or aggrieving things.②the person(s) or object(s) or event(s) that your are/were afraid of.③the past events about love relationship and sex that you are reluctant or feel too ashamed to mention.④the most trusted, dependence, unforgettable person, and why.⑤the most relaxed , happy time, and why? It comes from the ancient Chinese emotional theory and modern TCM psychotherapy practice. Nowadays, this method is widely used to help doctors quickly collect the patient's mental condition, growth experience, and convenient for clinical treatment.

Addendum was added to designing thought of sleep cognition information module of thought imprint psychotherapy under low resistant state (TIP) in this paper on the basis of past treatment experience.The main added contents included expected treatment information module,and cognitive information module according to the next sleep situation and possible future events.We hoped to provide some help to the psychotherapy of insomnia or better use of TIP.

Objective To study the protective effect of hyperoxic liquid preconditioning in myocardial ischemia-reperfusion injury. Methods Thirty rabbits were randomly divided into three groups: group A undergoing all procedures except ischemia-reperfusion, group C receiving the routine infusion of hyperoxic liquid 15mL/kg for seven days, and group B receiving the routine treatment infusion of saline instead of hyperoxic liquid 15mL/kg. Seven days later, the myocardial ischemic reperfusion model (I/R) was fabricated, ECG and homodynamic index were monitored simultaneously. Plasma MDA and SOD level was assayed before ligation, 30min after ligation and 1, 2, 3h after reperfusion. Results Hyperoxic liquid preconditioning can lower ST segment and improve index of hemodynamics and significantly reduce myocardial infarction size in group C compared with group B((P

AIM: To detect the amount of heavy metals and As salt in Dangyao Tablets. METHODS: The method for detecting the heavy metals and As salt in volume 1 of 2000 China Pharmacopoeia. RESULTS: The(amount) of heavy metals in 15 batches of Dangyao Tablets was little less than 20.0 mg/g;As salt was little less than 2.0 mg/g. CONCLUSION: The amount of heavy metals and As salt in Dangyao Tablets is in the limit of the standard.

Objective To investigate the cardio-protective effects of fructose-1,6-diphosphate on myocardium during coronary artery bypass graft.Methods A total of 30 patients were treated with coronary artery bypass graft(CABG) and divided into group A(treated group) and group B(control group) randomly.Each group included 15 patients.In the treated group,patients were treated with FDP three days before CABG and treated with FDP before cardiopulmonary bypass(CPB).FDP was replaced by 0.154mol/L NaCl of the same volume in control group.Blood samples were collected from all patients after induction and at 1h,4h,24h and 48h after aortic declamping to measure the serum concentration of cTnI,CK-MB,SOD and MDA.Results The levels of CK-MB and cTnI at 1h,4h,24h and MDA at 1h,4h after reperfusion were significantly lower in the treated group than those in the control group(P

Objective To investigate the changes in gastric intramucosal pH(pHi) and the effect of hyperoxic liquid on intramucosal pH(pHi) after myocardial ischemia-reperfusion injury in rabbits.Methods Twenty healthy male adult rabbits,weighing 2.3-3.0 kg,were randomly divided into two groups: control group(n=10) and hyperoxic liquid group(n=10).Hyperoxic liquid group received the routine infusion of hyperoxic liquidle of 10 mL/kg for seven days,while control group received the routine treatment infusion of saline instead of hyperoxic liquid of 10 mL/kg.Seven days later,the myocardial ischemic reperfusion model(I/R) was established,and ECG and homodynamic indexes were monitored simultaneously.Hemodynamic indexes and pHi were measured before myocardial ischemia,30 min after myocardial ischemia as well as 1 h and 2 h after reperfusion.Results Hyperoxic liquid could lower ST segment and improve hemodynamic indexes.pHi decreased significantly after myocardial ischemia-reperfusion injury in both groups.Compared with that in control group,pHi was significantly higher at 1 h and 2 h after reperfusion in hyperoxic liquid group(P

Objective To evaluate the effect of early clinical interference strategies on preventing the conversion of acute pancreatitis to the severe form and aggravation of severe acute pancreatitis(SAP).Methods The patients with acute pancreatitis admitted to this hospital were divided into two therapeutic phases by different therapeutic methods from January 2001 to December 2008.Patients in the first phase(from January 2001 to December 2004) were treated by the routine management,and the second phase(from January 2005 to December 2008) by the routine management combined with early clinical interference strategies.Then,the ratio of conversion from acute pancreatitis to SAP and prognosis of SAP between two phases were compared.Results Compared with the first phase,the rate of aggravation of acute pancreatitis was significantly decreased in the second phase(4.48% vs.21.18%),the average healing time of SAP,the incidences of systemic and local complications and the mortality of pancreatitis were reduced(P

Many patients suffering from insomnia,especially chronic insomnia are treated by hypnotics.While taking hypnotics for long time will lead to drug dependence.How to fulfill gradual reduction of hypnotics has become one of the big problems doctor meet clinically.Drug dependence can be classified into two aspects.One is psychological,the other is pathological.Psychological treatment has advantage in the procedure of reducing drug.Thought imprint psychotherapy under lower resistance (TIP) which combines traditional Qigong,relaxation and cognitive-behavioral therapy of modern clinical psychology is a psychotherapy fit for Chinese.TIP techniques used during reduction procedure of hypnotics have been improved.

AIM: To observe the difference of cerebral injury following ischemia/reperfusion and mRNA expression of TGF-β1 between diabetic and non-diabetic rats.METHODS: At first, Wistar rats were divided into two groups,non-diabetes and diabetes, and then two groups followed by sham, middle cerebral artery occlusion(MCAO) 2h and reperfusion 24 h after MCAO 2 h respectively. TGF-β1 mRNA expression was measured by semi-quantitative reverse transcription polymerase chain reaction(RT-PCR); Cerebral damage was evaluated by histopathology.RESULTS: In the same condition of ischemia or ischemia/reperfusion, injuried area enlarged in DM groups; The expression level of TGF-β1 mRNA increased at the time of 2 h after MCAO in non-diabetic group and diabetic group, especialy significantly in non-diabetic group with MCAO 2 h, and decreased at the time of reperfusion 24 h after MCAO 2 h, but still higher than that in the sham group.CONCLUSION: Diabetes mellitus exacerbated brain lesion following ischemia/repefusion, increased TGF-β1 mRNA expresion after MCAO may be an anti-injury reaction,and anti-injury ability is decreased under diabetic condition