ObjectiveTo investigate the interaction pr otein with Wnt signaling molecular-TCF4 in renal carcinogenesis.Me thodsThe N terminal and DNA binding domain of human T cell factor 4 (TCF4ND, 1～495 aa) was used as a bait to screen the renal carcinoma cDNA libra r y by yeast two hybrid.Results51 positive colonies were fished out such as ?-catenin,TCF4,activating transcription factor 5(ATF5),and 2 unknown genes.ConclusionsIt is indicated that exten sive signaling cross-talks between Wnt and other signaling pathways might play an significant role in renal carcinogenesis.

ObjectiveTo investigate the differential expression of ATF5--a novel gene fished out by yeast two hybrid from renal carcinoma library.MethodsRT-PCR and Northern blot was used to examine the expression level of ATF5 in renal cell line A498,786-O,GRC-I,293 and HK-2.The area absorbance value of ATF5 RNA was quantitative by computerized-imaging system.ResultsCompared with the controls,the expression level in renal cancer cell line(A498,786-O,GRC-I) and embryonal cell line (293)is much higher than that in the normal cell line HK-2.ConclusionsIt is indicated that ATF5 may play a significant role in the renal carcinogenesis through cross-talk with Wnt signal pathway.

This paper reported that the activation of oncogenes in human fetal esopha geal epithelium treated by alternariol (AOH). It was found that NIH/3T3 cells were transformed via transfeetion of DNA extracted from human fetal esophageal epithelium which was cultured and treated by 10?g/ml AOH in a short term in vitro. The efficiency of primary loci was 0.17 focus per ?g of DNA. In the secondary transfection, the efficiency was 0.58 focus per ?g of DNA (P

OBJECTIVETo investigate peripheral blood apelin levels in patients with acute ST-elevation myocardial infarction (STEMI) and their correlation with the one-year outcome of the patients.

METHODSA total of 153 consecutive patients, including 93 with acute STEMI undergoing primary percutaneous coronary intervention (PCI), 30 with acute STEMI and 30 with stable angina all undergoing elective PCI, and 10 healthy control subjects were examined for peripheral blood apelin levels and clinical parameters. The composite endpoints (CEPs) were determined at the one year follow-up.

RESULTSApelin levels were significantly decreased in all the patients at admission, but increased following primary PCI. Apelin levels showed a negative correlation with glycosylated hemoglobin levels. At one year following PCI, the patients with a lower apelin level showed an increased risk for lowered left ventricular ejection fraction ratio, but further analysis failed to provide evidence that apelin levels were predictive of the one-year outcome.

CONCLUSIONPeripheral blood apelin levels might be useful for predicting the clinical outcomes of patients with acute STEMI.

Acute Disease ; Apelin ; Biomarkers ; blood ; Case-Control Studies ; Humans ; Intercellular Signaling Peptides and Proteins ; blood ; Myocardial Infarction ; blood ; diagnosis ; Percutaneous Coronary Intervention ; Prognosis ; Ventricular Function, Left

OBJECTIVETo enhance the knowledge and the effect of the diagnosis and treatment of primary epithelioid sarcoma of the penis.

METHODSOne rare case of primary epithelioid sarcoma of the penis was studied with regard to its primary clinical process and characteristics, differential diagnosis and method of treatment.

RESULTSAn operation was performed on the penis to treat the epithelioid sarcoma. The diagnosis was confirmed by immunohistological and pathological techniques. There was no evidence of relapse during the three-year follow-up after operation.

CONCLUSIONThe possibility of primary epithelioid sarcoma of the penis should be considered if a mass or induration of the proximal penis and the symptoms of urethremphraxis are found. Total phallectomy could be chosen as an appropriate method of treatment. Unless adenopathy is palpable, node dissections are not recommended.

Adult ; Diagnosis, Differential ; Humans ; Male ; Penile Neoplasms ; diagnosis ; pathology ; surgery ; Sarcoma ; diagnosis ; pathology ; surgery

Objective To investigate peripheral blood apelin levels in patients with acute ST-elevation myocardial infarction (STEMI) and their correlation with the one-year outcome of the patients. Methods A total of 153 consecutive patients, including 93 with acute STEMI undergoing primary percutaneous coronary intervention (PCI), 30 with acute STEMI and 30 with stable angina all undergoing elective PCI, and 10 healthy control subjects were examined for peripheral blood apelin levels and clinical parameters. The composite endpoints (CEPs) were determined at the one year follow-up. Results Apelin levels were significantly decreased in all the patients at admission, but increased following primary PCI. Apelin levels showed a negative correlation with glycosylated hemoglobin levels. At one year following PCI, the patients with a lower apelin level showed an increased risk for lowered left ventricular ejection fraction ratio, but further analysis failed to provide evidence that apelin levels were predictive of the one-year outcome. Conclusion Peripheral blood apelin levels might be useful for predicting the clinical outcomes of patients with acute STEMI.

Objective To investigate effects of oxidized low density lipoprotein/β2 glycoprotein-Ⅰ/anti-β2 glycoprotein-Ⅰ antibody(oxLDL/β2GPⅠ/aβ2GPⅠ)complex on the proliferation,migration and angiogenesis of vascular endothelial cells and its mechanism.Methods Human umbilical vein endothelial cells(HUVEC)were cultured to logarithmic growth phase and grouped into the control group(normal culture),the oxLDL group(50 mg/L oxLDL),the oxLDL/β2GPⅠ/aβ2GPⅠ group(50 mg/L oxLDL/100 mg/L β2GPⅠ/100 mg/L aβ2GPⅠ)and the VEGF group(100 μg/L VEGF).The gene expressions of VEGF,vascular endothelial cadherin(VE-cadherin),matrix metalloproteinase(MMP)-2 and MMP-9 were detected by real-time quantitative fluorescent PCR(qPCR).Cell counting kit-8(CCK-8)method was employed to detect cell proliferation.Cell migration and invasion were determined by scratch healing test and Transwell assay.Matrigel tube formation assay was used to observe the angiogenesis of HUVEC.The relative protein expression of TLR4,MyD88 and NF-κB were examined by Western blot assay.Results Compared with the control group,the proliferation activity of cells at 48 h of treatment was increased in the oxLDL/β2GPⅠ/aβ2GPⅠ group(P＜0.05).Moreover,compared with the control group,cell migration and angiogenesis were increased in the oxLDL/β2GPⅠ/aβ2GPⅠ group,and the mRNA levels of VEGF,VE-cadherin,MMP-2 and MMP-9 were elevated(P＜0.05).Compared with the control group,levels of TLR4 and MyD88 were elevated in the oxLDL/β2GPⅠ/aβ2GPⅠ complex group(P＜0.05),as well as levels of p-NF-κB p65/NF-κB p65(P＜0.05).Conclusion oxLDL/β2GPⅠ/aβ2GPⅠ complex may promote the proliferation,migration and tube formation of vascular endothelial cells by regulating TLR4/MyD88/NF-κB signaling pathway.

Objective To investigate peripheral blood apelin levels in patients with acute ST-elevation myocardial infarction (STEMI) and their correlation with the one-year outcome of the patients. Methods A total of 153 consecutive patients, including 93 with acute STEMI undergoing primary percutaneous coronary intervention (PCI), 30 with acute STEMI and 30 with stable angina all undergoing elective PCI, and 10 healthy control subjects were examined for peripheral blood apelin levels and clinical parameters. The composite endpoints (CEPs) were determined at the one year follow-up. Results Apelin levels were significantly decreased in all the patients at admission, but increased following primary PCI. Apelin levels showed a negative correlation with glycosylated hemoglobin levels. At one year following PCI, the patients with a lower apelin level showed an increased risk for lowered left ventricular ejection fraction ratio, but further analysis failed to provide evidence that apelin levels were predictive of the one-year outcome. Conclusion Peripheral blood apelin levels might be useful for predicting the clinical outcomes of patients with acute STEMI.

OBJECTIVETo demonstrate molecular insight into the pathology of Peyronie's disease (PD). A preliminary profile of differential gene expression between the PD plaque and control tunica albuginea was obtained with DNA microarrays. Also, to investigate the effect of intervention in PD cells, transforming growth factor-beta1 (TGF-beta1) was recruited to treat PD cell lines.

METHODSThree PD plaques and control tunica albugineas were constructed and studied. cDNA probes were prepared from RNA isolated from those cells and hybridized with the Clontech Atlas 3.6 Array. Relative changes of greater than 2.0 defined up-regulation and down-regulation, respectively. The expression of selected individual gene MCP-1 and the effect of TGF-beta1 on MCP-1 were analyzed by reverse transcriptase-polymerase chain reaction.

RESULTSSome up-regulated genes in the PD plaque detected by the Clontech assay were screened, one of them was monocyte chemotactic protein. One involved the pathogenesis of PD as a downstream gene and responded to the TGF-beta1 treatment but not CTGF. The results were also confirmed by TR-PCR in all the types of cell.

CONCLUSIONSThe cell lines from plaque tissue and normal tunica from men with PD were successfully established. The findings indicate a potential role for MCP-1 over expression in the pathogenesis of PD as a downstream gene regulated by some genes and could be a new therapeutic target in PD. The information may allow a better understanding of the basic mechanisms involved in the etiology and pathogenesis of PD. Furthermore, it may permit some strategies of therapeutic interventions combine routine methods with Chinese herbal medicine.

Cell Line ; Chemokine CCL2 ; Gene Expression ; drug effects ; Gene Expression Profiling ; Humans ; Male ; Oligonucleotide Array Sequence Analysis ; Penile Induration ; drug therapy ; genetics ; pathology ; Proteins ; genetics ; Reverse Transcriptase Polymerase Chain Reaction ; Transforming Growth Factor beta ; pharmacology

Objective: To account the direct cost of uterine cervix carcinoma treatment in China and to explore the related factors which influence the direct financial burden of the disease. Methods: Data was collected through the medical record system and telephone interviews in 14 county-level hospitals and 9 provincial and municipal hospitals from 14 provinces/municipalities enrolled in the Chinese National Health Industry Research Project in 2015. The direct financial burden of uterine cervix carcinoma treatment consisted of the direct medical cost and the direct non-medical cost of treatment in different pathological cervical cancer stages and precancerous lesions. Multiple liner regression method was used to analyze the factors affecting the costs. Results: The age of the 3 246 patients was (46.40±10.43) years, including 2 423 patients from provincial and municipal hospitals and 823 patients from county-level hospitals. The direct financial burden for one patient of pathological uterine cervix carcinoma stage or precancerous lesion ranged from 10 156.3 yuan to 75 716.4 yuan in provincial and municipal hospitals, and for patients from county-level hospitals, the cost was between 4 927.9 yuan and 47 524.8 yuan per person. There was a wide gap between the direct financial burden of patients in different disease stages. The direct financial burden of patients with precancerous lesions ranged from 4 927.9 yuan per person to 11 243.0 yuan per person, as for patients of pathological uterine cervix carcinoma stages, the direct financial burden was between 29 274.6 yuan and 75 716.4 yuan per person. The factors which influence direct financial burden would include: the levels of the hospital, pathological period, medicare reimbursement, days of treatment, and the methods of treatment (P<0.001). Conclusion The direct financial burden of diseases in patients with pathological uterine cervix carcinoma stage or precancerous lesion differed in different levels of hospital and pathological periods. In addition, medicare reimbursement, days of treatment, and the methods of treatment all had impact on it.