Objective To establish an immunohistochemical method for postmortem diagnosis of electrocution. Method 12 rats were divided into two groups,experimental and control. In the experimental group,6 rats were killed by electricity,and in the control group,6 rats were killed by breaking the rats' neck. Myocardium sections were then made and were stained by SABC immunohistochemical method as well as HE.Results The expression of the fibronectins in the experimental rats' myocardia were significantly increased as compared with those in the control rats (P

AIM: To determine if aquaporin-1 (AQP-1) expression and function is influenced after lipopolysaccharide (LPS) stimulation in rat lung microvessel endothelial cells and to investigate the mechanisms of lung fluid abnormal metabolism in acute lung injury. METHODS: LPS at different concentrations (100 ?g/L, 1 mg/L or 10 mg/L) was used to stimulate cultivated rat lung microvessel endothelial cells in vitro at different stimulatory times (4 h, 12 h or 24 h), respectively. Tritium water permeation was conducted for determining the intracellular signal intensity in rat lung microvessel endothelial cells. The RT-PCR technique was applied for the assay of the expression of AQP-1 mRNA. RESULTS: The signal intensity of intracellular tritium water in the LPS stimulation group was less than that in normal control significantly. Compared with the normal control group (P

BACKGROUND: After the treatment of osteoporotic vertebral compression fractures with bone cement implantation,deteriorative osteoporosis and the distance augment between bone cement and bones result in a decrease in bone cement-bone interface compression strength and torsion strength, and then the bone cement tends to displace, which is easy to cause a secondary vertebral fracture.OBJECTIVE: To investigate the clinical efficacy of bone cement implantation via three approaches on osteoporotic vertebral compression fractures.METHODS: Sixty patients with single osteoporotic vertebral compression fracture were treated with vertebroplasty,percutaneous kyphoplasty or fraction cement vertebroplasty. All data were collected to analyze the correlation between the bone cement morphology and treatment method, and to detect the visual analogue scale, Oswestry disability index,Cobb angle and incidence of secondary vertebral fractures.RESULTS AND CONCLUSION: The visual analogue scale scores, Oswestry disability index, and Cobb angle in thethree groups were significantly improved after treatment (P < 0.05). All above indicators showed no significant difference among groups at each time point after treatment, suggesting that these three kinds of treatments exert the same effects on pain relief, functional recovery, preventing the vertebral height loss and maintaining spinal mechanical properties.Bone cement displacement and secondary vertebral fracture occurred after clotted cement implantation. Therefore,mixed and trabecular-shaped grafts are preferred, aiming to achieve good long-term treatment outcomes in osteoporotic vertebral compression fractures.

Objectives: To elucidate the prognostic impact of preoperative control of nutritional status (CONUT) scores on gastric cancer patients undergoing adjuvant chemotherapy after radical gastrectomy. Methods: A retrospective analysis of 536 stage Ⅱ-Ⅲ gastric cancer patients undergoing adjuvant chemotherapy after radical resection from Jul 1998 to Dec 2014 was performed. Patients were divided into high (≥3) and low (≤2) CONUT groups with a CONUT score of 3 divided into critical values. Results: The 5-year survival rate of the high CONUT group was significantly lower than that of the low CONUT group (37.3 % vs. 55.7%, P<0.001). Univariate analysis showed that the high CONUT group was associated with larger tumors, more lymph node metastasis, lower body mass index, higher prognostic nutritional index, and preoperative anemia (all P<0.05). Multivariate analysis showed that the CONUT score was an independent prognostic factor for patients with gastric cancer (HR: 1.564, 95% CI: 1.090-2.321, P=0.016). The 5-year survival rate of the high CONUT group was significantly lower than that of the low CONUT group (P<0.05). Conclusion The CONUT score is an indicator for predicting the prognosis of patients with stage Ⅱ-Ⅲ gastric cancer after adjuvant chemotherapy. The nutritional evaluation is helpful to develop a plan for preoperative nutritional intervention.

Humans ; SARS-CoV-2 ; COVID-19 ; Antibodies ; Antibodies, Viral/therapeutic use* ; Antibodies, Neutralizing/therapeutic use*