OBJECTIVE:To determine streptomycin sulfate and sulfonamide in compound streptomycin cream by UV spectrophotometry.METHODS:The maltol-ammonium ferric sulfate colourimetry was adopted in which the ammonium ferric sulfate was taken as the developer,the absorbability of streptomycin sulfate was determined with the wavelength at521nm;The absorbability of sulfonamide was determined at a wavelength of251nm with0.1%mol/L NaOH taken as the sol-vent.RESULTS:The linear ranges of streptomycin sulfate and sulfonamide were200～950?g/ml(r=0.9994)and1.464～7.784?g/ml(r=0.9998)respectively;Their respective average recovery were99.1%(RSD=1.90%)and99.7%(RSD=1.60%).CONCLUSION:The method can be used as the quality control for compound streptomycin cream.

Objective To investigate motor patterns in the upper limbs of C_5 to C_6 spinal cord injury (SCI) patients during elbow extension. Methods Fifteen C_5 and C_6 SCI patients and fifteen healthy subjects formed an SCI group and a control group respectively. A three-dimensional kinematic analysis was performed on videos of the subjects performing four elbow extension activities: grasping a cup, reaching for a light switch, propelling a wheelchair, and upper limb weight bearing. A Peak Motus motion analysis system was employed. Results Compared with controls, the movement time of the SCI group was significantly longer during grasping, reaching for a light switch and wheelchair ambulation. Their angular velocity was significantly slower during reaching for a light switch and during wheelchair ambulation. The patients with spinal cord injury performed the motor tasks primarily by relying on increasing or decreasing the angular displacement of the shoulder. But they compensated for their functional deficiency by changing the movement direction of the shoulder and elbow in sagittal projection during upper limb weight bearing activities. Conclusion C_ 5-6 SCI patients use motor patterns and strategies different from those of control subjects in completing elbow extension activities.

Objective To study upper limb motor strategies during elbow extension activities in C5-6 spinal cord injury (SCI) patients and compare them with the strategies of normal individuals. Methods A Peak Motus motion analysis system was used to measure the angular displacement and the angular velocity of the shoulder, elbow and wrist of fifteen C5-6 SCI patients and fifteen healthy subjects during four elbow extension activities including grasping a cup, reaching for a light switch, wheelchair ambulation and upper limb weight-bearing. Results Compared with normal individuals, the movement time of the SCI patients was significantly longer during the grasping, reaching and wheelchair ambulation activities. The angular velocity was significantly slower during reaching for a light switch and wheelchair ambulation. The patients with spinal cord injury performed the motor tasks primarily relying on increasing or decreasing the angular displacement of the shoulder during the former three elbow extension activities. They compensated for any functional deficiency by changing the movement direction of the shoulder and elbow in sagittal projection during upper limb weight-bearing activities. Conclusion C5-6 SCI patients utilize different motor patterns and strategies from healthy subjects to complete elbow extension activities.

Amounts of tanshenoside Ⅰ in cultivated Ludangshen of different years of cultivation history was determined by TLC densitometry in comparison with that in wildly grown Ludangshen and cultivated Taidangshen. Results showed that tanshenoside Ⅰ in cultivated Ludangshen decreases with increased years of cultivation history while that in cultivated Taidangsihen is slightly lower than that of Ludangshen of the same years of cultivation. Tanshenosde Ⅰ in wild Taidangshen is also lower than that in cultivated Taidangshen

Objective To access the effect of intermittent catheterization (IC) on the urinary tract infection in patients with spinal cord injury. Methods Sixty patients of spinal cord injury combined with urinary tract infec-tion were randomly divided into two groups: treatment group (32 patients) treated with sensitive antibiotics and IC;control group (28 patients) treated with sensitive antibiotics only. Both groups were treated for two weeks. Results In the treatment and control group,the cure rate was 56% and 29% ,respectively,the total effective rate was 91% and 64% in the treatment and control group,respectively,the clearance rate of bacteria was 81% and 57% ,respec-tively. All differences were significant (P<0.05). Conclusions IC can improve the effect of antibiotic on urinary tract infection in patients with spinal cord injury, and might be effective on the treatment of urinary tract infection caused by various neurogenic bladders.

BACKGROUND:It is vital to choose the appropriate carrier with low toxicity and high gene transfection efficiency in gene therapy, which is harmless to human body and environment. OBJECTIVE: To prepare superparamagnetic Fe3O4/SiO2-polyethyleneimine (PEI) composite particles. METHODS: Fe3O4 nanoparticles were prepared via an emulsion solvent evaporation method and superparamagnetic Fe3O4/SiO2 core shel microspheres were prepared successfuly subsequently via a modified stober method. The microspheres were further modified with PEI to obtain superparamagnetic Fe3O4/SiO2-PEI composite particles. The structures and properties of resultant composite particles microspheres were characterized by transmission electron microscopy, zeta potential and vibrating sample magnetometer. Superparamagnetic Fe3O4/SiO2-PEI composite particles were mixed with plasmid DNA at different mass ratios (29∶1, 39∶1, 49∶1, 59∶1, 68∶1, 78∶1, 88∶1). Thein vitro gene transfection ability was evaluated by Hela cels with the transfection of plasmid DNA encoded with green fluorescent protein and the transfection efficiency was determined by confocal fluorescence microscopy. RESULTS AND CONCLUSION: We successfuly synthesized the Fe3O4/SiO2-PEI composite particles with good dispersibility and even size distribution (about 100 nm). The surface charge was 21.07 mV, and the saturation magnetization was 28.05 emu/g that meant superparamagnetism. When the mass ratio was 59∶1, al the plasmid DNA was adherent to the Fe3O4/SiO2-PEI composite particles; when the mass ratio was > 59∶1, there were excessive Fe3O4/SiO2-PEI composite particles. Therefore, the mass ratio of 59:1 could lead to a better outcome for HeLa celltransfection. These results indicate that the Fe3O4/SiO2-PEI composite particles can dramaticaly improve the transfection efficiency of plasmid DNA compared with PEI.

AIM:To determine gentianaceae and jasminoidin in Longhui Capsules (Radix et Rhizoma Gentia-nae, Aloe, Fructus Gardeniae, etc.) METHODS: The Waters Symmetry C_ 18 column (4.6 mm?150 mm, 5 ?m) was used with mobile phase of methanol-water (17∶83). The flow rate was 1.0 mL/min. The detecting wavelength was at 254 nm, and the column temperature was at 30 ℃. RESULTS: The methodological study showed that a good linear correlation existed in the range of 60.72-454.4 ng for gentianaceae and the range of 96.32 -722.40 ng for jasminoidin. The average recovery of gentianaceae was 99.57%(n=9) and RSD was 1.27% , jasminoidin was 99.10%(n=9) and RSD was 2.30%. CONCLUSION: The method is simple, accurate and can be used for quality control of the preparation.

Objective:To explore the influence of mindfulness meditation intervention on the professional identity and turnover tendency of the second victim of nurses.Methods:The convenience sampling method was used to select the second victim of 46 nurses who experienced medical adverse events within six months from January to June 2019 in Zhongnan Hospital of Wuhan University, and then an 8 weeks of mindfulness meditation intervention was carried out for them. In the entire intervention process, a self-control study was conducted, the Second Victim Experience and Support Scale (SVSET) and the Nurse Occupational Identity Scale were used to assess nurses' turnover intentions, absence and professional identity before the intervention and at 2 hours, 4 weeks, and 8 weeks after the intervention.Results:By analyzing the data, the results showed that after the mindfulness meditation intervention, the second victim of the nurse before intervention, 2h, 4 weeks, and 8 weeks of intervention were 3.28±0.87, 3.20±0.83, 2.85±0.92, 2.71±0.87, the difference was statistically significant ( F time value was 6.609, P<0.05). Before and after the intervention of the sense of grasp in professional identity were 4.91±0.83, 5.47±0.81, 5.83±0.91, 5.94±0.80, the difference was statistically significant ( F time value was 20.973, P<0.01). Consensus before and after intervention were 4.61±0.78, 5.09±0.85, 5.54±1.12, 5.76±0.85, the difference was statistically significant ( F time value was 22.237, P<0.01). The meaningful sense before and after intervention were 4.38±1.03, 5.04±0.75, 5.44±0.86, 5.59±0.76, the difference was statistically significant ( F time value was 23.656, P<0.01). The self-efficacy before and after intervention were 4.71±0.82, 5.33±0.63, 5.83±0.84, 5.90±0.77, the difference was statistically significant ( F time value was 28.866, P<0.01). The self-determination before and after intervention were 4.43±0.97, 5.06±0.81, 5.59±0.91, 5.62±0.87, the difference was statistically significant ( F time value was 25.237, P<0.01). Before and after intervention, the influence of tissue was 4.17±0.96, 4.71±0.85, 4.96±0.99, 5.30±0.88, the difference was statistically significant ( F time value was 16.487, P<0.01). Before and after intervention, the patients' feelings were 4.59±0.96, 5.08±0.84, 5.70±0.83, 5.83±0.68, the difference was statistically significant ( F time value was 25.406, P<0.01). Conclusion:This article provides an effective method to help nurses' second victims out of their predicament which confirms that mindfulness meditation intervention can effectively reduce turnover tendency of nurses 'second victims and improve their professional identity.

Objective To investigate arsenic trioxide (As2O3)-mediated apoptosis of synovlal cells in pa-tients with rheumatoid arthritis (RA) through culturing the synoviocytes in vitro. Methods Primary synovial cells were cultured by means of two-enzymatic digestion and the third cells were adopted in this test. The cultured cells were defined by MTT and flow cytometry (FCM). Results Certain concentration of As2O3 could inhibit the viability of synoviocytes at 48 h by means of MTT, which was dose-dependent. Certain concentration of As2O3 could induce the apoptosis of synoviocytos pro rata at 48h by means of FCM ,which was dose-dependent within range of 10-80 μmol/L concentration. Conclusion Certain concentration of As2O3 following 48 h effect could induce the apoptosis of syno-viocytes of RA,which is dose-dependent.

Objective To evaluate the serum organochlorine pesticides residues levels in breast cancer pa-tients with different estrogen receptor(ER). Methods 93 patients of breast cancer from Fengnan districtand 65 pa-tients from Tanghai county in Tangshan were selected,whose ER were detected by pathology and serum organochlo-fine pesticides (DDTs and HCHs) levels were detected and compared with gas chromatography/electron capture. Results The orders of detection rate of HCH isomer in breast cancer patients in two areas were β-HCH >δ-HCH > α-HCH >γ-HCH. The orders of detection rate of DDT isomer in breast cancer patients in two areas were PP'-DDE > PP'-DDT > OP'-DDT > PP'-DDD. The serum levels of β-HCH, PP'-DDE in breast cancer patients in two are-as were positively correlated with age (r=0.272 ,0.330 , P<0.01) or BMI (r=0.312,0.187, P <0.01, <0.05). There were statistical differences in the serum β-HCH,δ-HCH,PP'-DDE levels between the estrogen receptor posi-tive breast cancer patients and the estrogen receptor negative breast cancer patients in two areas (P<0.05 for each). Conclusion The serum organochlorine pesticides residues levels in breast cancer patients with positive es-trogen receptor are higher than that with negative estrogen receptor, implicating that breast cancer is dependent on es-trogen. The cause of breast cancer is likely due to estrogen-like effect of serum organochlorine pesticides residues.