Objective To investigate clinical value of high sensitive-cardiac troponin T(hs-cTnT) combined with creatine kinase isoenzyme MB(CK-MB) in the diagnosis of children with myocarditis.Methods From Nov.2014 to Nov.2015,a total of 102 cases of myocarditis,suspected with myocardial damage and without myocardial damage(pneumonia and capillary bronchitis),and 50 healthy children were enrolled.Plasma levels of hs-cTnT and CK-MB were detected and compared.Results The levels of plasma hs-cTnT and CK-MB in children with myocarditis were significantly higher than those without myocarditis and healthy subjects(P<0.05).Hs-cTnT and CK-MB levels in children with myocarditis,less than one month old,were significantly higher than those with age of 1 month to 3 years old(P<0.05).Conclusion Combined detection of hs-cTnT and CK-MB could be with high sensitive and specificity in diagnosis of children with myocarditis,accurately assess the disease condition and improve the therapeutic effect and prognosis,which might be worthy of clinical application.

Objective To observe the clinical effect of DuoDERM combined with AquacelAg dressing on the wound after the xanthelasma palpebrarum treatment with laser surgery .Methods One hundred and fourteen patients after the xanthelasma palpebrarum treatment with laser surgery were chosen and were randomly divided into three groups .All patients received the clean of wound surface using the same method , and dressing change according to the wound .The observation group ( n =39 ) received the DuoDERM combined with AquacelAg dressing on the wound , and the control group Ⅰ( n=37 ) received the DuoDERM dressing alone , and the control group Ⅱ( n=38 ) received the traditional gauze dressing change that was sterile gauze to cover wound surface ,keep clean and dry .The growth time of granulation on the wound , the healing time , the scar hyperplasia and the cure rate were compared among three groups .Results The growth time of granulation on the wound and the healing time were respectively (5.35 ±2.18) d, (11.15 ±2.24) d in the observation group, and were better than those of the control group , and the differences were statistically significant ( F=9.000, 5.717, respectively;P<0.05).The cases of scar hyperplasia and the cure rate were respectively 0 cases and 100%in the observation group , and were better than those of control group , and the differences were statistically significant (χ2 =13.515,21.351, respectively; P<0.01).Conclusions The clinical effect of DuoDERM combined with AquacelAg dressing on the wound after the xanthelasma palpebrarum treatment with laser surgery is better than the DuoDERM dressing and the traditional gauze dressing change alone , and is worthy of clinical promotion.

OBJECTIVETo evaluation the specificity and sensitivity of 5 kinds of serological detection methods about brucellosis.

METHODSTo investigate in the 4 autonomous banner (Cha You Hou Qi, Right-Wing Central Banner of Kerqin Region, Linxi County and Siziwangqi Banner) of Inner Mongolia autonomous region from January to December, 2013. Accepting criteria: professionals of breeding cattle and sheep, and slaughter,accompanied by Bloom's disease suspected symptoms such as fever, fatigue,arthralgia, ranging in age from 25 to 55 years old. To collect suspected patients venous blood 3-5 ml in the morning, a total of 236 samples were collected. To detect the Brucella antibody by using plate agglutination test (PAT), tiger red plate agglutination test (RBPT), standard test tube agglutination test (SAT), enzyme-linked immunosorbent assay (ELISA) and immune colloidal gold method (GICA), SAT was taken as a golden standard, analyzed the sensitivity and specificity of RBPT and SAT, ELISA and GICA.

RESULTSSAT method of positive patients: 136 cases (57.6%). PAT method positive patients: 150 cases (63.6%). RBPT positive patients: 159 cases (67.4%), and 143 patients with ELISA method: positive (60.6%), 147 patients with positive GICA method (62.3%). The detection rate of Brucella antibody positive was different by different testing methods.There was no significant difference (χ(2)=0.52,P=0.264). To take the SAT method as the gold standard, PAT, RBPT, ELISA and GICA method of the sensitivity were 97.7% (133/136), 98.5% (134/136), 94.8% (129/136) and 94.1% (128/136), respectively. The specificity was lower,the rate were 70.0% (70/100), 75.0% (75/100), 86.0% (86/100) and 81.0% (81/100), respectively. The total coincidence rate were 86.0% (203/236), 88.5% (209/236), 91.1% (215/236) and 88.5% (209/236), respectively.

CONCLUSIONThe specificity and sensitivity of ELISA and GICA method is higher in the diagnosis of disease. The two methods are rapid, GICA method can be used on-site testing, large sample test is suitable for using ELISA.

Adult ; Agglutination Tests ; methods ; Animals ; Antibodies, Bacterial ; blood ; Brucella ; Brucellosis ; diagnosis ; Cattle ; China ; Enzyme-Linked Immunosorbent Assay ; Humans ; Middle Aged ; Sensitivity and Specificity ; Sheep