OBJECTIVETo investigate the chemical constituents of the roasted seeds of Cassia obtusifolia to illuminate the change of its effective components before and after roasted.

METHODCompounds were separated by silica gel chromatography, and their structures were evaluated by spectral analysis and chemical evidence.

RESULTSeven compounds were isolated from the ethanol extract. Their structures were identified as chrysophanol (1), physcion (2), 8-methoxylchrysophanol (3), beta-sitosterol (4), emodin (5), obtusin (6) and obtusifolin-2-O-beta-D-(6'-O-acetyl) glucopyranside (7).

CONCLUSIONCompounds 1-7 were isolated from the roasted seeds of C. obtusifolia for the first time, and compound 7 was a new compound.

Cassia ; chemistry ; Cooking ; Hot Temperature ; Organic Chemicals ; analysis ; isolation & purification ; Seeds ; chemistry

OBJECTIVETo establish the HPLC fingerprint method and compare the changes of chemical compositions of the processed products from Sinapis alba.

METHODThe procedure of HPLC analysis was performed on a agilent TC-C18 (2) column at 35 degrees C with the acetonitrile -0.1% phosphoric acid in gradient elution as the mobile phase. The detection wavelength was set at 254 nm, and the flow rate was 1.0 mL x min(-1). The similarity evaluation system for chromatographic fingerprint of traditional Chinese medicine (Version 2004 A) was applied to analyze the similarity.

RESULTThe average similarities among the processed products were over 0.96, the standard HPLC fingerprint and five main chromatographic peaks with the isolated compounds was obtained and identified.

CONCLUSIONThe established methods with two solvents are suitable for the HPLC fingerprints determination which elementary elucidate the scientific intensions of breaking the enzyme for glycosides.

Chemistry, Pharmaceutical ; Chromatography, High Pressure Liquid ; methods ; Drugs, Chinese Herbal ; analysis ; Sinapis ; chemistry

OBJECTIVETo establish HPLC fingerprint for the identification of Cassia obtusifolia and compare chemical constituents of the raw and roasted seeds of C. obtusifolia.

METHODChromatographic fingerprint of C. obtusifolia was investigated by RP-HPLC and the gradient elution mode was applied to chromatographic separation. Data were analyzed by fingerprint similarity evaluation software to compare the similarity of the samples for identifying the main chromatographic peaks furthermore.

RESULTHaving compared the HPLC fingerprint of the raw and roasted seeds of C. obtusifolia were compared preliminarily, and 12 main chromatographic peaks were identified.

CONCLUSIONHPLC method can be used in quality control and identification of the raw and roasted seeds of C. obtusifolia.

Cassia ; chemistry ; Chromatography, High Pressure Liquid ; Cooking ; Drugs, Chinese Herbal ; chemistry ; Hot Temperature ; Seeds ; chemistry

OBJECTIVETo establish methods of RP-HPLC respectively for content-determination of two types of constituents in the Cassia obtusifolia respectively, and tp determine the constituents between the raw and roasted seeds of C. obtusifolia in order to distinguish the difference of those seeds.

METHODHPLC systems consisted of Alltima C18 (4.6 mm x 150 mm, 5 microm), column temperature at 30 degrees C, flow rate of 1.0 mL x min(-1). Two different mobile phases and detection wavelengths: I , ACN-THF-0.1% H3 PO4 (17 : 3:80), 278 nm; II , MeOH-0.1% H3PO4 (79:21), 254 nm. Analyzed and compared the content of the two types of constituents between the raw and roasted seeds of C. obtusifolia.

RESULTThe calibrate curves of 5 constituents were linear (r > 0.999 7). The precision and repeatability were perfect (RSD < 1.6%, 1.8%). The samples were stabile during 24 h.

CONCLUSIONThe study provide a better universal reference for evaluating and controlling the quality of C. obtusifolia pieces.

Cassia ; chemistry ; Chromatography, High Pressure Liquid ; Drugs, Chinese Herbal ; analysis ; Hot Temperature ; Seeds ; chemistry

OBJECTIVETo identificate and compare constituents in the HPLC fingerprint of five Chinese herbal pieces from Radix et Rhizoma Rhei.

METHODHPLC analysis was carried out with methyl alcohol 1% glacial acetic acid as gradient elution, changes in five Chinese herbal pieces and medicinal material under 280 nm and 430 nm were compared.

RESULTHPLC fingerprints of the no-parched pieces, the liquor and the vinegar roasts pieces were similar, 24 peaks were identified under 280 nm 19 or 22 peaks could be indicated in the braising with liquor and the charring respectively. Under 430 nm, 8 peaks were identified except the braising with liquor.

CONCLUSIONHPLC fingerprints of the no-parched pieces, the liquor and the vinegar roasts pieces are similar while the changes on chemical composition and the content in braising with liquor and the charring are remarkable.

Chromatography, High Pressure Liquid ; Drugs, Chinese Herbal ; analysis ; Rheum ; chemistry

OBJECTIVETo compare the contents of five anthraquinone components in five different processed products from Rheum palamatum.

METHODThe contents of aleo-emodin, rhein, emodin, chrysophanol and physcion were determined simultaneously by HPLC on plogilent TC-C18 (2) column at 35 degrees C with the methanol-0.1% phosphoric acid (85: 15). The detection wavelength was set at 254 nm and the flow rate was 1.0 mL x min(-1).

RESULTThe obtained linearity of the five components was better over 0.999 9 and the average recoveries were 96.44%, 98.11%, 99.30%, 98.00% and 97.86%, respectively.

CONCLUSIONThe results showed the remarkable variation regulations show in the five different processed products. Compared to the no-parched pieces, the contents of the five anthraquinone components have evidently increased in the braising with liquor and the charring products, and reduced in the vinegar and the liquor sauted pieces.

Anthraquinones ; analysis ; Pharmaceutical Preparations ; analysis ; Rheum ; chemistry

Objective:To prepare monoclonal antibodies specific to human papillomavirus type 6 L1 (HPV6 L1) protein, investigate the binding characteristics, cross-reactivity, and neutralization efficacy of these antibodies.Methods:Antibody genes were extracted from the spleen cells of mice immunized with HPV6 L1 protein and used to construct an ScFv phage antibody library. The library was enriched and screened using HPV6 L1 protein. Following expression of the antibody genes into murine IgG, the binding properties of the antibodies to the L1 protein and their neutralization effects on pseudoviruses were verified.Results:An ScFv antibody library targeting HPV6 L1 was successfully constructed with a capacity of 6.54×10 7. After screening with HPV6 L1, two antibody strains were obtained, named Q9 and Q11. ELISA testing revealed that Q9-IgG has a high antibody titer (about 10 6) against HPV6 L1, while cross-reactivity titers to HPV18 and 45 L1 were 10 2. Q11-IgG has an antibody titer about 4×10 4 against HPV6 L1, but not combined with the L1 proteins of other HPV types. Pseudovirus neutralization assays indicated that Q9-IgG lacked neutralizing activity, while Q11-IgG showed a neutralizing antibody titer of 10 4.5against HPV6 pseudovirus. Conclusions:Both Q9 and Q11 are monoclonal antibodies specific to HPV6 L1. However, they displayed notable differences in cross-reactivity, antigen recognition sites, and neutralization efficacy. These antibodies provide essential tools for the foundational research of HPV6, immunological diagnosis, and the development of therapeutic formulations.