1.Anthraquinone contents in five processed products from Rheum palamatum.
Cun ZHANG ; Li LI ; Yongqing XIAO ; Na LIN ; Chunfang LIU ; Guiliu LI ; Zhen PANG ; Dongdong CHEN ; Guofang TIAN
China Journal of Chinese Materia Medica 2009;34(15):1914-1916
OBJECTIVETo compare the contents of five anthraquinone components in five different processed products from Rheum palamatum.
METHODThe contents of aleo-emodin, rhein, emodin, chrysophanol and physcion were determined simultaneously by HPLC on plogilent TC-C18 (2) column at 35 degrees C with the methanol-0.1% phosphoric acid (85: 15). The detection wavelength was set at 254 nm and the flow rate was 1.0 mL x min(-1).
RESULTThe obtained linearity of the five components was better over 0.999 9 and the average recoveries were 96.44%, 98.11%, 99.30%, 98.00% and 97.86%, respectively.
CONCLUSIONThe results showed the remarkable variation regulations show in the five different processed products. Compared to the no-parched pieces, the contents of the five anthraquinone components have evidently increased in the braising with liquor and the charring products, and reduced in the vinegar and the liquor sauted pieces.
Anthraquinones ; analysis ; Pharmaceutical Preparations ; analysis ; Rheum ; chemistry
2.Identification and comparison of constituents in HPLC fingerprint of five Chinese herbal pieces from Rheum palamatum.
Li LI ; Cun ZHANG ; Yongqing XIAO ; Na LIN ; Chunfang LIU ; Zhen PANG ; Guiliu LI ; Dongdong CHEN ; Guofang TIAN
China Journal of Chinese Materia Medica 2009;34(13):1668-1671
OBJECTIVETo identificate and compare constituents in the HPLC fingerprint of five Chinese herbal pieces from Radix et Rhizoma Rhei.
METHODHPLC analysis was carried out with methyl alcohol 1% glacial acetic acid as gradient elution, changes in five Chinese herbal pieces and medicinal material under 280 nm and 430 nm were compared.
RESULTHPLC fingerprints of the no-parched pieces, the liquor and the vinegar roasts pieces were similar, 24 peaks were identified under 280 nm 19 or 22 peaks could be indicated in the braising with liquor and the charring respectively. Under 430 nm, 8 peaks were identified except the braising with liquor.
CONCLUSIONHPLC fingerprints of the no-parched pieces, the liquor and the vinegar roasts pieces are similar while the changes on chemical composition and the content in braising with liquor and the charring are remarkable.
Chromatography, High Pressure Liquid ; Drugs, Chinese Herbal ; analysis ; Rheum ; chemistry
3.Preparation of neutralizing monoclonal antibodies against human papillomavirus type 6 L1 protein using phage display
Weizi QIN ; Guiliu CHEN ; Li ZHANG
Chinese Journal of Experimental and Clinical Virology 2024;38(1):67-71
Objective:To prepare monoclonal antibodies specific to human papillomavirus type 6 L1 (HPV6 L1) protein, investigate the binding characteristics, cross-reactivity, and neutralization efficacy of these antibodies.Methods:Antibody genes were extracted from the spleen cells of mice immunized with HPV6 L1 protein and used to construct an ScFv phage antibody library. The library was enriched and screened using HPV6 L1 protein. Following expression of the antibody genes into murine IgG, the binding properties of the antibodies to the L1 protein and their neutralization effects on pseudoviruses were verified.Results:An ScFv antibody library targeting HPV6 L1 was successfully constructed with a capacity of 6.54×10 7. After screening with HPV6 L1, two antibody strains were obtained, named Q9 and Q11. ELISA testing revealed that Q9-IgG has a high antibody titer (about 10 6) against HPV6 L1, while cross-reactivity titers to HPV18 and 45 L1 were 10 2. Q11-IgG has an antibody titer about 4×10 4 against HPV6 L1, but not combined with the L1 proteins of other HPV types. Pseudovirus neutralization assays indicated that Q9-IgG lacked neutralizing activity, while Q11-IgG showed a neutralizing antibody titer of 10 4.5against HPV6 pseudovirus. Conclusions:Both Q9 and Q11 are monoclonal antibodies specific to HPV6 L1. However, they displayed notable differences in cross-reactivity, antigen recognition sites, and neutralization efficacy. These antibodies provide essential tools for the foundational research of HPV6, immunological diagnosis, and the development of therapeutic formulations.