Objective To observe the effects ofHuolisu oral liquid combined with sertraline on the serum levels of norepinephrine (NE) and 5-hydroxytryptamine (5-HT) in patients with postpartum depression due to Qi-Blood deficiency, and to explore therapeutic efficiency.Methods A total of 82 patients with postpartum depression due to Qi-Blood deficiency were randomly divided into a treatment group with 43 patients and a control group with 39 patients. The patients in the treatment group were treated withHuolisu oral liquid and sertraline, and those in the control group were treated with sertraline alone for 8 weeks. Severity of depression was evaluated with the Hamilton Depression Scale (HAMD), and the serum levels of NE and 5-HT was detected.Results The HAMD scores after the treatment for 8 weeks were significantly lower than before the treatment in both groups (4.73 ± 3.57vs. 21.49 ± 3.20 in the treatment group, 9.11 ± 3.13vs. 21.11 ± 3.12 in the control group; allP<0.05). The serum levels of NE (137.89 ± 56.81 pg/mlvs. 96.19 ± 36.48 pg/ml in the treatment group, 127.71 ± 54.27 pg/mlvs.96.15 ± 34.33 pg/ml in the control group; allP<0.05) and 5-HT (153.43 ± 24.89 ng/mlvs. 45.03 ± 9.09 ng/ml in the treatment group, 125.31 ± 25.09 ng/mlvs. 44.46 ± 8.17 ng/ml in the control group; allP<0.05) after the treatment for 8 weeks were significantly higher than before the treatment in both groups. After the treatment for 8 weeks, the HAMD score was significantly lower, the serum levels of NE and 5-HT were significantly higher in the treatment group than those in the control group (allP<0.05). ConclusionsHuolisu oral liquid combined with sertraline can significantly decrease HAMD score, and increase serum levels of NE and 5-HT, obviously improve the depression symptoms in patients with postpartum depression due to Qi-Blood Deficiency.

Objective To explore a layered nursing intervention based on risk assessment strategies application effect in elderly patients with hip replacement. Methods Chosen during January 2013 to December 2014 rows of orthopaedic trauma treated 224 patients of senile hip replacement as the research objectto divide into observation group and control group 112 caseseach according to admission time.Observation group applied based on risk assessment strategy stratification nursing intervention, the control group used conventional care. Compared two groups of patients with postoperative pain degree, hip function, complications, and patient satisfaction. Results For 24 h, 48 h, 72 h after the operation, Visual Analog Scale (VAS) scores of observation groupwere 2.25 ± 0.35, 2.01 ± 0.31, 1.92 ± 0.23, which were significantly lower than 3.53 ± 0.51, 2.84±0.45, 2.70±0.35 of the control group (t=21.900, 16.075, 21.900, P<0.01);3 months and 12 months after the surgery, the index of the Harris hip score observation group was 71.32 ± 7.45, 93.44 ± 9.65, which were obviously higher than 60.47 ± 6.65, 83.75 ± 9.21 of control group (t=11.498, 7.688, P<0.01);8.04%(9/112) incidence of complications such as infection of incision, increase of observation group was obviously lower than 20.54%(23/112) of the control group(χ2=7.146, P<0.05);97.32%(109/112) satisfaction was significantly higher than 78.57% (88/112) of the control group (χ2= 18.572, P < 0.05). Conclusions Stratified nursing intervention based on risk assessment strategy can help to alleviate the degreeof pain, improve hip function, reduce postoperative complications and improve patient satisfaction.

BACKGROUND: Sliding cupping has been used for treating cervical spondylosis, with the effects of dredging the meridian system, activating qi and removing blood stasis.OBJECTIVE: To probe into symptomatic improvement of cervical spondylosis with sliding cupping and the relationship between curative effect and course of disease as well as age.DESIGN: Self controlled observation.SETTING: Department of Physiotherapy, General Hospital of Air Force of Chinese PLA.PARTICIPANTS: Totally 100 out-patients with cervical spondylosis receiving treatment in the Department of Physiotherapy, General Hospital of Air Force of Chinese PLA were involved from December 2000 to December 2003. Among them, 39 cases were male and 61 cases were female; 9 cases were under 30 years old, 38 cases were 31 to 40 years old, 42 cases were 41 to 50 years old and 11 cases were above 50 years old. There were 36 cases with less-than-one-year course of disease, 49 with 1 to 5-year course and 15 with more-than-5-year course. Fifty-one cases were cervical type; 25 cases vertebral artery type; 24 cases nerve root type. X-ray examination revealed that there were 69 cases whose cervical vertebral physiological curvature became straight or reverse, or with certain degree of degenerative change. Vertebroarterial blood supply insufficiency was detected in 23 cases by rheoencephalogram.INTERVENTIONS: The patients were asked to be in prone sitting position,their nape and shoulder were fully exposed, and the negative pressure cupping was applied to patient' s nape and shoulder until petechiae appeared on the skin. The treatment was given once or twice a week. After one-month treatment, the curative effect was calculated according to subjective symptoms, signs, and the results of X-ray and rheoencephalogram examination before and after treatment. Symptom disappearance: pain was 0 grade; the cervical vertebral physiological curvature restored by 10°- 15° and rheoencephalogram showed that the contraction peak velocity of the vertebroarterial blood supply became normal. Marked improvement: the pain reduced by more than 2 grade, the cervical vertebral physiological curvature restored by 6°9° and rheoencephalogram showed that the vertebroarterial blood supply restored to 80% of the normal range. Improvement: the pain reduced by 1 grade, the cervical vertebral physiological curvature restored by 3°- 5° and rheoencephalogram showed that the vertebroarterial blood supply restored to 40% - 80% of the normal range. Inefficacy: the pain did not reduce, the cervical vertebral physiological curvature restored by less than 3° and rheoencephalogram showed that the vertebroarterial blood supply became less than 40%.RESULTS: According to the intention-to-treat analysis, 100 patients entered the result analysis. For symptom disappearance, marked improvement,improvement, inefficacy there were 27, 17, 5 and 2 cases respectively in cervical type; 8, 10,4 and 3 cases respectively in the vertebral artery type;4,9,7 and 4 cases respectively in the nerve root type. There were 19, 12, 3 and 2 cases respectively with less-than-one-year course of disease; 18, 19, 7 and 5 cases respectively with 1 to 5-year course; 2, 5, 6 and 2 cases respectively with more-than-5-year course. And there were 5, 3 and 1 cases respectively with age ＜ 30 years; 18, 13, 4 and 3 cases respectively with age of 31 to 40 years; 15, 18, 5 and 4 cases respectively with age of 41 to 50years; 1, 2, 6 and 2 cases respectively with age ＞ 50 years.CONCLUSION: The effect of sliding cupping was good for patients with cervical spondylosis of vertical type, next of vertebroarterial type and bad of nerve root type. The shorter the course of disease is, the younger the patient is, and the better the curative effect is.

The natural process of life and death has been profoundly and widely involved and increasingly manipulated by the fast developed technology of life science,which could change the human beings' state of life as well as the state of death.Thus,human beings are motivated to think multidimensionally on life and death from the perspective of science,such as the weakening of sacredness of life,the technology employed in life,the socialization of lifestyles,the exploration of the nature of death,the pluralism of different ways of death and the systematization in the education of death.These considerations will no doubt contribute to the mutual promotion of the development of morality and the advancement of life science technology.

Objective To discuss the change of bone resorption of primary nephrotic syndrome (PNS) patients with prednisone treatment, andα-D3 effects on it. Methods ①30 PNS patients diagnosed and 28 healthy people in our hospital were selected;blood and urine samples before and after enough prednisone (pred) treatment were collect-ed to detect the iPTH, calcium and urinary DPD. ②30 PNS patients after 6 weeks enough prednisone treatment were randomly divided into Pred +α-D3 group and Pred group. Results ① PNS group compared with healthy group, urine DPD excretion rate was significantly increased(P<0.05),and blood iPTH was significantly increased (P<0.01);blood calcium was similar to healthy group. ② PNS patient after treatment with pred compared with that before treatment, urine DPD excretion rate was significantly increased(P<0.01),and blood iPTH was signifi-cantly increased(P<0.01). There was no obvious change in blood calcium.③Pred+α-D3 group compared with Pred group, urine DPD excretion rate was significantly decreased(P<0.01),and blood iPTH was significantly de-creased(P<0.05). There was no obvious change in blood calcium. Conclusion α-D3 can effectively reduce bone resorption in PNS patients.

Objective To study the clinical effect and value of the combination of dexamethasone and calcium agent assisted oxytocin on preventing postpartum hemorrhage.Methods 128 cases of maternal of clinical information were reviewed,and which were divided into control group and observation group randomly.The control group was immediately infused muscle note 20IU of Oxytocin and the observation group(n =64) was given 5％ of glucose and 100ml 10％ of glucose acid 10ml calcium.And then give 20IU oxytocin after pending.Lastly,give the two groups comparison of blood-producing capacity and the number of cases of blood-producing.Results Ater observation groups in fetal foetus blood-producing capacity of 2h and 24h,the number of cases of blood-producing less than the control group,and both have significant statistical differences (all P ＜ 0.01).Conclusion The combination of dexamethasone and calcium agent-assisted application of oxytocin can prevent postpartum hemorrhage,better clinical effect,which should be promot in clinical application.

Objective To explore the safety and effectiveness of dexamethasone and calcium gluconate combined with oxytocin to prevent postpartum hemorrhage.Methods 128 maternal patients with full-term delivery were divided the patients into two parts randomly:the control group and the observation group,of which contained three groups(A,B,C).The patients were all given 20 u oxytocin.As to observation,group A:before 10-15min of cutting skin,give 10mg of dexamethasone and 10 ml 10％ calcium gluconate rapid intravenous infusion,group B:give 10mg intravenous infusion of dexamethasone after babies were boro,group C:before 10 ～ 15min of cutting skin,give 10mg of dexamethasone and 10ml 10％ calcium gluconate rapid intravenous infusion and then give 10mg dexamethasone after babies were born.The control group wasn't given that medicine,and only give them 20 u oxytocin when babies were born.At last,give contrast of total amount of bleeding,ill reflecting of medicine and the other symptom after 24h.Results The amount of bleeding of observation group A,B,C were (277.3 ± 44.5) ml,(286.8 ± 52.7) ml,(301.8 ±60.9) ml,respectively.The amount of bleeding of the control group was (422.9 ± 78.2) ml.The observation group and control group had significant differences(t =-0.699,0.481,-0.407,all P ＜ 0.01),and there was no significant difference between group A,B,and C (P ＞ 0.05).And this two groups had no bad reflection and the other symptoms.Conclusion Dexamethasone and calcium gluconate combined with oxytocin to prevent postpartum hemorrhage has great efficacy and safety.This method must be extended.

OBJECTIVE To investigate the knowledge about hand hygiene among the healthcare workers in the infectious disease hospital and improve their hand hygiene compliance.METHODS A standardized questionnaire was sent to 364 healthcare workers in the infectious disease hospital.The following data investigated anonymously were collected.RESULTS All the healthcare workers' answers to the choice questions were right(100.0%).However,there were significant differences in the answers to the memorized questions among the healthcare workers with different careers.The correct answers among the nurses were the highest(97.7% and 94.8%,respectively);among the doctors were 71.4% and 34.7%;and among the medical technicians were 21.5%,and 8.6%,respectively.But there were no significant differences between sexes,education degrees and service length in the three groups.CONCLUSIONS The healthcare workers of our hospital are well in the field of basic knowledge about hand hygiene.However,there are differences in the answers to the memorized questions among healthcare workers with different careers.It need to enhance the systems of training and examination for workers,especially for the doctors,technicians and all the new staff.Alcohol-based hand rub(AHR),which is prone to practice,would replace hand-washing.And it should be more supervised.

Objective To explore the roles of miR-10a in the cisplatin resistance in cervical cancer cell lines,and further study the mechanism.Methods The cells were transfected with the mimics of miR10a and its negative control RNA (NC).Methyl thiazolyl tetrazolium (MTF) assay and fluorescence activated cell sorter were used to analyze drug sensitivity and apoptosis after treating with cisplatin,luciferase reporter assay to identify that miR-10a directly targets phosphatase and tensin homologue deleted on chromosome ten (PTEN).The expression of PTEN gene and its protein levels after tansfection was measured,respectively.Results after transfected with miR-10a mimics:(1) The 50％ inhibition concentration of Hela cells (7.2 μg/ml) was more than in the NC group (5.6 μg/ml).The 50％ inhibition concentration of Siha cells (6.4 μg/ml) was significantly more than in the NC group (3.8 μg/ml) (P＜0.05).(2) The apoptosis rates of Hela cells and Siha cells were significantly lower compared to the NC group (P ＜ 0.05).(3)miR-10a might directly target the 3'-untranslated region (3'-UTR) of PTEN,which significantly changed the PTEN protein level (P ＜ 0.05).Conclusions miR-10a may modulate cisplatin-induced apoptosis in cervical cancer by inhibiting PTEN protein level.

Objective To explore the expression of anaplastic lymphoma kinase(ALK) in human ovarian serous adenocarcinoma cell line SKOV3 and to further investigate the effect of crizotinib on SKOV3 cells.Methods The expression of ALK in SKOV3 cells were examined by Western blotting and immunocytochemical methods,and the effect of ALK inhibitor crizotinib on proliferation of SKOV3 cells were evaluated by cell proliferation assay.Results Both Western blotting and immunocytochemical methods showed the expression of ALK in SKOV3 cells.The results of cell proliferation assay suggested that the cell viability of SKOV3 cells was close to 1 when the drug concentration was less than 106 nmol·L-1,and close to 64% when the drug concentration was up to 107nmol·L-1.Conclusion ALK is overexpressed in human ovarian serous adenocarcinoma cell line SKOV3,but SKOV3 cells were insensitive to the therapy of ALK inhibitor crizotinib.