1.Simultaneous Determination of Phenylpropanoids in Drug Pairs of Chuanxiong Rhizoma and Angelicae Sinensis by HPLC
China Pharmacist 2015;18(10):1718-1720
Objective: To determine the content of phenylpropanoids in the drug pairs of Chuanxiong Rhizoma and Angelicae Sinensis. Methods:An ISC-HPLC method was used to determine the content of ferulic acid, protocatechuic acid and chlorogenic acid. Results:The content of ferulic acid, protocatechuic acid and chlorogenic acid in the drug pairs of Chuanxiong Rhizoma and Angelicae Sinensis all had good linear relationship with the peak area within the range of 0. 90-14. 40,1. 25-20. 00, and 1. 00-16. 00μg·ml-1 , respectively, and the average recovery was 97. 16%, 94. 98% and 98. 14%, respectively (n=9). Conclusion:The HPLC method is simple, rapid, accurate and reproducible, which can be used for the quality control of the drug pairs of Chuanxiong Rhizoma and An-gelicae Sinensis.
2.Circulating MicroRNAs and Inflammatory Bowel Disease
Pei DENG ; Min MIN ; Yan LIU ; Guiliang WANG ; Yang XU
Chinese Journal of Gastroenterology 2016;21(2):118-120
MicroRNAs(miRNAs)are a group of non-coding RNA molecules having modulating function,and as a post transcriptional modulating factor is involved in the modulation of expression of eukaryotic genes. Inflammatory bowel disease(IBD)is a chronic non-specific intestinal inflammatory disease and its etiology has not yet been fully clarified. Recent studies have shown that circulating miRNAs were specifically expressed in patients with IBD. This article reviewed the advances in studies on circulating miRNAs and IBD.
3.MiR-630 inhibits MDA-MB-231 cells migration and invasion by targeting Sox4 in triple-negative breast cancer
Yan SHI ; Yan QIN ; Lei SONG ; Yuemian LIANG ; Xiaolei WANG ; Guiliang HAN
Chongqing Medicine 2017;46(6):773-776,779
Objective To verify whether miR-630 could inhibit MDA-MB-231 cells migration and invasion by targeting Sox4 in triple-negative breast cancer(TNBC).Methods Collection normal breast tissue and breast cancer tissue from patients undergoing breast cancer resection.RT-PCR were used to test the expression of miR-630,miR-21,miR-195,miR-134,miR-200a,miR-381 and miR-1228.Western blot were used to test the expression of COL1A1,COL1 A5,MMP-2,MMP-9 and Sox4.In vitro experiment,after miR-630 was transfected into MDA-MB-231 cells,wound healing were employed to test the migratory ability of MDA-MB-231 cells,and transwell were used to test the invasion ability of MDA-MB-231 cells.Western blot were used to investigate the expressions of COL1 Al,COL1 A5,MMP-2,MMP-9 and Sox4 in MDA-MB-231 cell.Luciferase assay was used to confirmed whether Sox43'-UTR the target gene of miR-630.Results Compared with normal breast tissue,the expression of miR-630 was decreased(P<0.01),meanwhile the expression of COL1A1,COL1A5,MMP-2,MMP-9 and Sox4 were significantly increased in the triple-negative breast cancer tissue(P<0.01).In the vitro experiment,compared with the control group,the expression of COL1A1,COL1A5,MMP-2,MMP-9 and Sox4 were decreased in the miR-630 group (P<0.05);The migration activity of MDA-MB-231 cells was decreased in the miR-630 group (P<0.01);The Luciferase activity of the Sox4-3'-UTR plasmid was significantly suppressed by miR630 (P<0.05);Over expression of Sox4 could reverse the effect of miR-630 on MDA-MB-231(P<0.05,P<0.01).Conclusion In triple-negative breast cancer tissue,the expression of miR-630 decreased;miR-630 inhibits triple-negative breast cancer cells migration and invasion by targeting Sox4-3’-UTR.