OBJECTIVE: To construct hospital pharmaceutical website and facilitate the evolvement of hospital pharmaceutical care.METHODS: Website management system was developed independently and the system was used to construct hospital pharmaceutical website in our hospital.RESULTS & CONCLUSIONS: Website management system which was designed with B/S three-tier structure which sustains multi-level management authority control and a variety of database and runs stably.The professional website based on website management system is convininent and maintenance,rapid in operation,cheap in cost,low in consumption of human resouces,comprehensive in function,highly professional,and it has greated enhanced the working efficiency in hospital pharmacy.

OBJECTIVE:To study the bioequivalences of both domestic nevirapine capsules and imported nevirapine tablets.METHODS:24healthy male volunteers were administered orally with single dose of either the domestic nevirapine trial capsules or the imported nevirapine reference tablets by randomized crossover way,the blood concentration of nevirapine was determined by HPLC,and the pharmacokinetics parameters and the relative bioavailability of whom were calculated.RE?SULTS:The C max of the trial preparation and the reference preparation were(2.516?0.446)?g/ml and(2.798?0.394)?g/ml,respectively t max were(7.5?10.7)and(3.6?2.1)h,t 1/2 were(51.4?25.3)h and(46.4?9.8)h;AUC 0～168 were(160.540?38.007)(?g?n)/ml and(167.459?30.629)(?g?n)/ml;AUC 0～∞ were(180.064?51.005)(?g?n)/ml and(183.052?36.828)(?g?n)/ml;The relative bioavailability of the trial preparation was(98.368?22.99)%.CONCLUSION:The results shows that the trial preparation and the reference preparation of nevirapine were bioequivalent.

OBJECTIVE:To develop the drug traceability management system in outpatients of medical institutions,and gradu-ally improve its traceability management. METHODS:Based on barcode technology,drug traceability management system in outpa-tients of medical institutions was independently developed,introducing it from system environment,framework design and system function,and the application results were evaluated in terms of differences in dispensing time,deployment error,drug withdrawal treatment and applicability investigation. RESULTS:The system was developed on hospital information system network environ-ment,which was designed by combination of client/server(C/S)network system(B/S)and set function modules as follows as role rights management,data extraction,information control,data acquisition and data query. The system can match with drug electron-ic surveillance code,commodity code and other traceable barcodes,achieve drug tracing from hospitals to users through software interaction,as well as the computer-aided calibration in dispensing to effectively reduce outpatient's dispensing error. The average time for each outpatient prescription prolonged 9 s after using the system;24 dispensing errors and 4 non-normal withdrawals were prevented within 1 month;100% surveyed pharmacists expressed approval for the system's applicability. CONCLUSIONS:The system can achieve the drug suitability management in outpatients,which has shown good applicability and further improved drug safety management and control capabilities in medical institutions.

OBJECTIVE:To determine ampicillin concentration in human plasma by HPLC.METHODS:The chromatographic column was Waters Sunfire C18 with mobile phase consisted of acetonitrile and 0.05 mol?L-1 potassium dihydrogen phosphate(6.5∶93.5,V∶V)at a flow rate of 1.2 mL?min-1.The UV detection wavelength was 210 nm;the column temperature was 35 ℃ and the injection volume was 20 ?L.RESULTS:The linear range of ampicillin was 0.2～16.0 ?g?mL-1(r=0.999 9).The limit of quantization was 0.2 ?g?mL-1.The average methodological recovery was 99.88%～104.45%,and the average extraction recovery was 95.69%～100.56%.Both the inter-day RSD and the intra-day RSD were all less than 10%.CONCLUSION:This method is stable,rapid and sensitive,and suitable for the pharmacokinetic study for ampicillin preparation.

OBJECTIVE:To esbablish pre-dispensing management mode and computer system in outpatient dispensary to facilitate the practice of hospital pharmaceutical care.METHODS:The establisment and application of the pre-dispensing management mode and computer system in outpatient dispensary were analyzed from aspects of hardware preparation,design of system framework and functional modules of the system.RESULTS:The application of pre-dispensing management system brought down the dispensing error rate and reduced patients' waitting time.The stability of the computer system was the key to ensure smooth operation of the pre-dispensing management mode.CONCLUSION:The practice of the pre-dispensing management mode facilitates the formation of good pharmaceutical care system and is a good dispensing managment mode suitable for sound development of hospital pharmacy.

Alcoholic liver disease(ALD)is the most frequent liver disease worldwide,resulting in severe harm to personal health and posing a serious burden to public health.Based on the reported antioxidant and anti-inflammatory capacities of scutellarin(SCU),this study investigated its protective role in male BALB/c mice with acute alcoholic liver injury after oral administration(10,25,and 50 mg/kg).The results indicated that SCU could lessen serum alanine aminotransferase(ALT)and aspartate aminotransferase(AST)levels and improve the histopathological changes in acute alcoholic liver;it reduced alcohol-induced malondialdehyde(MDA)content and increased glutathione peroxidase(GSH-Px),catalase(CAT),and superoxide dismutase(SOD)activity.Furthermore,SCU decreased tumor necrosis factor-α(TNF-α),interleukin-6(IL-6),and IL-1β messenger RNA(mRNA)expression levels,weakened inducible nitric oxide synthase(iNOS)activity,and inhibited nucleotide-binding oligomerization domain(NOD)-like receptor protein 3(NLRP3)inflammasome activation.Mechanistically,SCU suppressed cytochrome P450 family 2 subfamily E member 1(CYP2E1)upregulation triggered by alcohol,increased the expression of oxidative stress-related nuclear factor erythroid 2-related factor 2(Nrf2)and heme oxygenase-1(HO-1)pathways,and suppressed the inflammation-related degradation of inhibitor of nuclear factor-κB(NF-κB)-α(IκBα)as well as activation of NF-κB by mediating the protein kinase B(AKT)and p38 mitogen-activated protein kinase(MAPK)pathways.These findings demonstrate that SCU protects against acute alcoholic liver injury via inhibiting oxidative stress by regulating the Nrf2/HO-1 pathway and suppressing inflammation by regulating the AKT,p38 MAPK/NF-κB pathways.

Objective: To investigate the differential expression of serum-and-glucocorticoid-inducible-kinase-2 (SGK2) in hepatocellular carcinoma (HCC) and normal liver tissues and the related mechanism mediating signal transduction of GSK-3 β / β catenin in HCC cells. Methods: Twenty pairs of matched HCC and normal tissues were collected and the situation of expression of SGK2 mRNA was detected by real-time fluorescence quantitative PCR. Western blot was used to detect the levels of SGK2 protein in human HCC cell lines (Huh-7, SMMC-7721) and normal human liver cell line (L02). SGK2 siRNA was used to transfect human HCC cell lines (SMMC-7721 and Huh-7), and then the protein expression levels of GSK-3 β/ β - catenin was successfully detected with the above-mentioned transfected cell line by western blot. Measurement data were expressed as mean ± standard deviation (±s), and the Student t -test was used as the statistical method. Results: SGK2 mRNA expression was up-regulated in all 20 HCC samples than that of the expression of matched normal liver tissues. SGK2 protein levels were significantly higher in Huh-7 and SMMC-7721 than normal human liver cell lines (P < 0.01). The downregulation of SGK2 expression in human HCC cell lines (SMMC-7721 and Huh-7) had inhibited the expression of unphosphorylated GSK-3 β. In addition, the downregulation of SGK2 expression in HCC cell lines had decreased the dephosphorylation of β - catenin to prevent degradation of the β - catenin proteasome. Conclusion SGK2 is overexpressed in HCC and mediates GSK-3β/β- catenin signaling in HCC cells.